Objective: To observe the analgesic effect of acupuncture and moxibustion on chronic constriction injury (CCI) pain model rats, and to investigate the mechanism of constriction constriction injury. Methods: 32 SPF SD ...Objective: To observe the analgesic effect of acupuncture and moxibustion on chronic constriction injury (CCI) pain model rats, and to investigate the mechanism of constriction constriction injury. Methods: 32 SPF SD rats were divided into Control Group, Sham Surgery Group, Model Group and Acupuncture and moxibustion Group, with 8 rats in each group. The Model Group, Acupuncture and moxibustion Group and sciatic nerve ligation were used to establish the CCI pain rat model. The sham operation group only separated the nerve, and the control group did not do any treatment. After modeling, the Acupuncture and moxibustion Group was given acupuncture treatment, while the control group and the Model Group did not do any intervention. 7 days for 1 course of treatment, continuous treatment for 3 courses of sampling and detection. Paw withdrawal mechanical threshold (PWMT) and Paw withdrawal thermal latency (PWTL) were observed. Biochemical detection of reactive oxygen species (ROS), malondialdehyde (MDA), Super Oxide Dismutase (SOD), glutathione (GSH);Serum, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels were detected by ELISA. Results: Compared with the control group, PWMT and PWTL in the Model Group were decreased (P α, IL-1β and IL-6 levels or contents were increased (P α, IL-1β and IL-6 levels or contents were decreased (P Conclusion: Acupuncture and moxibustion can reduce the ROS level, enhance the antioxidant capacity of the body, reduce the damage of sciatic nerve caused by inflammation, and relieve sciatic pain.展开更多
Hydrogen peroxide(H_(2)O_(2))has gained widespread attention as a versatile oxidant and a mild disin-fectant.Here,an electrostatic self-assembly method is applied to couple ZnSe quantum dots(QDs)with a flower-like cov...Hydrogen peroxide(H_(2)O_(2))has gained widespread attention as a versatile oxidant and a mild disin-fectant.Here,an electrostatic self-assembly method is applied to couple ZnSe quantum dots(QDs)with a flower-like covalent organic framework(COF)to form a step-scheme(S-scheme)photocata-lyst for H_(2)O_(2)production.The as-prepared S-scheme photocatalyst exhibits a broad light absorption range with an edge at 810 nm owing to the synergistic effect between the ZnSe QDs and COF.The S-scheme charge-carrier transfer mechanism is validated by performing Fermi level calculations and in-situ X-ray photoelectron and femtosecond transient absorption spectroscopies.Photolumi-nescence,time-resolved photoluminescence,photocurrent response,electrochemical impedance spectroscopy,and electron paramagnetic resonance results show that the S-scheme heterojunction not only promotes charge carrier separation but also boosts the redox ability,resulting in enhanced photocatalytic performance.Remarkably,a 10%-ZnSe QD/COF has excellent photocatalytic H_(2)O_(2)-production activity,and the optimal S-scheme composite with ethanol as the hole scavenger yields a H_(2)O_(2)-production rate of 1895 mol g^(-1)h^(-1).This study presents an example of a high-performance organic/inorganic S-scheme photocatalyst for H_(2)O_(2)production.展开更多
Advancements in the vehicular network technology enable real-time interconnection,data sharing,and intelligent cooperative driving among vehicles.However,malicious vehicles providing illegal and incorrect information ...Advancements in the vehicular network technology enable real-time interconnection,data sharing,and intelligent cooperative driving among vehicles.However,malicious vehicles providing illegal and incorrect information can compromise the interests of vehicle users.Trust mechanisms serve as an effective solution to this issue.In recent years,many researchers have incorporated blockchain technology to manage and incentivize vehicle nodes,incurring significant overhead and storage requirements due to the frequent ingress and egress of vehicles within the area.In this paper,we propose a distributed vehicular network scheme based on trust scores.Specifically,the designed architecture partitions multiple vehicle regions into clusters.Then,cloud supervision systems(CSSs)verify the accuracy of the information transmitted by vehicles.Additionally,the trust scores for vehicles are calculated to reward or penalize them based on the trust evaluation model.Our proposed scheme demonstrates good scalability and effectively addresses the main cause of malicious information distribution among vehicles.Both theoretical and experimental analysis show that our scheme outperforms the compared schemes.展开更多
Lithium sulfur batteries are regarded as a promising candidate for high-energy-density energy storage devices.However,the lithium metal anode in lithium-sulfur batteries encounters the problem of lithium dendrites and...Lithium sulfur batteries are regarded as a promising candidate for high-energy-density energy storage devices.However,the lithium metal anode in lithium-sulfur batteries encounters the problem of lithium dendrites and lithium metal consumption caused by polysulfide corrosion.Herein we design a dualfunction PMMA/PPC/LiNO3composite as an artificial solid electrolyte interphase(PMCN-SEI)to protect Li metal anode.This SEI offers multiple sites of C=O for polysulfide anchoring to constrain corrosion of Li metal anode.The lithiated polymer group and Li3N in PMCN-SEI can homogenize lithium-ion deposition behavior to achieve a dendrite-free anode.As a result,the PMCN-SEI protected Li metal anode enables the Li||Li symmetric batteries to maintain over 300 cycles(1300 h)at a capacity of 5 m Ah cm^(-2),corresponding to a cumulative capacity of 3.25 Ah cm^(-2).Moreover,Li-S batteries assembled with 20μm of Li metal anode(N/P=1.67)still deliver an initial capacity of 1166 m A h g-1at 0.5C.Hence,introducing polycarbonate polymer/inorganic composite SEI on Li provides a new solution for achieving the high energy density of Li-S batteries.展开更多
Wetland is one of the most important ecosystems with varied functions and structures,and its loss has been a major issue.Wetland loss in Modem Yellow River Delta(MYRD) becomes a serious environmental problem,so its re...Wetland is one of the most important ecosystems with varied functions and structures,and its loss has been a major issue.Wetland loss in Modem Yellow River Delta(MYRD) becomes a serious environmental problem,so its restoration attracts a great deal of attention from academia and governments.This article proposes a GIS-based multi-criteria comprehensive evaluation methodology for potential estimation of wetland restoration,using MYRD as an example.The model uses four kinds of data(hydrology,terrain,soil,and land use) and could be adapted by planners for use in identifying the suitability of locations as wetland mitigation sites at any site or region.In the application of the model in the MYRD,the research developed a lost wetland distributed map taking the better wetland situation of 1995 as the reference,and elevated the overall distribution trends of wetland restoration potential based on wetland polygon.The results indicated that the total area of wetland loss from 1995 to 2014 was 568.12 km^2,which includes 188.83 km^2 natural wetland and 21.80 km^2 artificial wetland,respectively.The areas of lost wetland with low,middle,and high resilience ability are 126.82 km^2,259.92 km^2,and 119.59 km^2,occupying 25.05%,51.33%,and 23.62%,respectively.The high-restoration-potential wetland included98.47 km^2 of natural wetland and 21.12 km^2 of artificial wetland,which are mainly bush,reed,and ponds.The highrestoration-potential wetland is mainly distributed in the vicinity of Gudong oil field,the Yellow River Delta protected areas,and the eastern sides of Kenli county and Dongying city.展开更多
Prostate mucinous adenocarcinoma with signet ring cells (MC-SRC) is a rare morphologic variant of prostate cancer, with only 12 cases reported to date. Diagnosis of this carcinoma requires that at least 25% of the tum...Prostate mucinous adenocarcinoma with signet ring cells (MC-SRC) is a rare morphologic variant of prostate cancer, with only 12 cases reported to date. Diagnosis of this carcinoma requires that at least 25% of the tumor tissue should consist of an extracellular mucin pool. In this report, we present a case of prostate prostate mucinous adenocarcinoma with signet ring cells.展开更多
Background: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damag...Background: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damage. At the same time, some studies have proved that Zhuangtongy in (ZTY) has a certain protective effect on the myocardium of rats with coronary heart disease. Therefore, the aim of this study is to observe and explore the effects of ZTY on cardiomyocytes and its mechanism of action in rats with coronary heart disease based on the Caspase-1 mediated cellular pyroptosis pathway. Methods: Fifty SD rats were randomly divided into blank group, sham-operation group, model group, ZTY group and cysteinyl aspartate specific protease (Caspase-1) inhibitor group. The blank group did not receive any treatment, and the other groups established a rat model of coronary artery disease by ligating the left anterior descending branch of the coronary artery, but the sham-operation group was only threaded without ligation. After that, the sham-operated and model groups were gavaged with 0.1 ml/kg distilled water, the ZTY group was gavaged with 13.6 g/kg ZTY decoction based on the previous study, and the Caspase-1 inhibitor group was injected intraperitoneally with 3 mg/kg Ac-YVAD-cmk solvent once a day for four consecutive weeks. Transmission electron microscopy was used to observe the ultrastructure of cardiomyocytes, HE staining was used to observe the morphology of cardiac tissue, and enzyme-linked immunosorbent assay (Elisa) was used to detect serum interleukin-1β (IL-1β), interleukin-18 (IL-18) and C-reactive protein (CRP) in the rats. In addition, fluorescent probe was used to detect serum Reactive oxygen species (ROS). Real-time fluorescence quantitative PCR was used to detect the expression of mRNAs of ASC, Nlrp3, Caspase-1 and gasdermin-D (GSDMD), and the protein expression of Caspase-1 and GSDMD was detected by immunohistochemistry. Results: There was no significant difference between the results of the blank and sham-operated groups. Compared with the blank group, the transmission electron microscopy results showed swollen and ruptured cardiomyocyte membranes in the model group, with pore formation, severe mitochondrial swelling, membrane lysis, and cristae breakage. The staining results showed myofibril breakage, severe intercellular oedema and vacuolation in the model group. The inflammatory factors IL-1β, IL-18, CRP and ROS were significantly elevated (P β, IL-18, CRP, ROS and the expression of ASC, Nlrp3, Caspase-1, and GSDMD mRNA were significantly elevated (P Conclusion: ZTY can reduce myocardial injury by lowering the expression of ROS and other inflammatory factors, inhibiting Caspase-1 mediated cellular pyroptosis, thus reducing myocardial inflammation and protecting cardiomyocytes.展开更多
Achievement of steady and reliable super-low friction at the steel/steel contact interface,one of the most tribological systems applied for mechanical moving parts,is of importance for prolonging machine lifetime and ...Achievement of steady and reliable super-low friction at the steel/steel contact interface,one of the most tribological systems applied for mechanical moving parts,is of importance for prolonging machine lifetime and reducing energy consumption.Here we reported that the superlubricity performance of the steel/steel sliding interface lubricated with tiny amounts of diketone solution strongly depends on the oxygen content in surrounding environment.The increase of oxygen not only significantly shortens the initial running-in time but also further reduces the stable coefficient of friction in superlubricity stage due to the enhancement of tribochemical reactions.On the one hand,more severe oxidation wear occurring at higher oxygen content facilitates material removal of the contact interface,lowering the contact pressure and the corresponding initial friction.On the other hand,the growth of iron ions during the shear process in high oxygen environment promotes the formation of chelate which acted as an effective lubricated film chemisorbed at the steel/steel friction interface to further lower the interfacial friction.The results provide a new opportunity to further optimize the tribological performance of diketone superlubricity system,especially towards the lubrication of mechanical engineering materials.展开更多
The offspring of super-multiparous sows face problems such as decreased growth performance,poor meat quality and even diseases in animal husbandry.Gama-aminobutyric acid(GABA)has long been known to promote growth and ...The offspring of super-multiparous sows face problems such as decreased growth performance,poor meat quality and even diseases in animal husbandry.Gama-aminobutyric acid(GABA)has long been known to promote growth and suppress inflammation,but little is known about the mechanisms.A total of 72 growing-finishing pigs from the 8th generation were randomly allotted to 2 groups with 6 replicates per treatment to receive a cornesoybean basal diet or the basal diet supplemented 20 mg/kg GABA for 60 d.After the animal-trial period,samples of serum and liver were collected for further analysis.Additionally,a lipopolysaccharide(LPS)-induced inflammatory model using HepG2 cells was established to explore the role of GABA on regulating hepatic inflammation.The results indicated that inflammatory cell infiltration occurs in the liver of progeny of super-multiparous sows,and dietary supplementation with GABA influenced liver morphology,increased activities of antioxidant enzymes and decreased the expression abundance of pro-inflammatory cytokines,including tumor necrosis factor-α(TNFα)and interleukin(IL)-1β,in the liver of growing-finishing pigs(P<0.05).In addition,GABA supplementation increased mRNA expressions of peroxisome proliferator-activated receptor g(PPARg)and GABA receptors(GABARs),and reduced the expression of toll-like receptor 4(TLR4)/nuclear factor-kB(NF-kB)signaling(P<0.05).Additionally,an in vitro experiment demonstrated that GABA decreased the expressions of hepatic TLR4/NF-kB signaling via activating GABARs under LPS-stress(P<0.05).In summary,liver injury may affect the growth performance of growing-finishing pigs by changing hepatic mitochondrial metabolism,the expression of pro-inflammatory cytokines and TLR4/NF-kB pathway and that GABA supplementation has a restorative effect by acting on GABARs.展开更多
Dear Editor,Taisui(太岁) has attracted great attention in China because it has been described in ancient Chinese texts as an entity possessing mysterious properties. In Chinese mythology and folklore, Jupiter is the G...Dear Editor,Taisui(太岁) has attracted great attention in China because it has been described in ancient Chinese texts as an entity possessing mysterious properties. In Chinese mythology and folklore, Jupiter is the God of the "Sui"(year) and is worshiped in Daoism. Jupiter appears at the same location in the sky approximately every 12 years(Jupiter’s orbit is11.86 years). However, since each cycle of Jupiter lasts for exactly 11.86 years and one Chinese year cycle comprises exactly 12 years, after approximately 84.7 years, Jupiter travels one cycle more than the number of cycles assumed by the ancient Chinese, which is called Taisui. According to the belief of the ancient Chinese, people are alerted when Taisui is digging in the ground, foretelling the destruction of houses or occurrence of natural disasters, such as floods and earthquakes(Zhao et al., 2018).展开更多
RNA interference(RNAi)is an intrinsic antiviral immune mechanism conserved in diverse eukaryotic organisms.However,the mechanism by which antiviral RNAi in mammals is regulated is poorly understood.In this study,we un...RNA interference(RNAi)is an intrinsic antiviral immune mechanism conserved in diverse eukaryotic organisms.However,the mechanism by which antiviral RNAi in mammals is regulated is poorly understood.In this study,we uncovered that the E3 ubiquitin ligase STIP1 homology and U-box-containing protein 1(STUB1)was a new regulator of the RNAi machinery in mammals.We found that STUB1 interacted with and ubiquitinated AGO2,and targeted it for degradation in a chaperon-dependent manner.STUB1 promoted the formation of Lys48(K48)-linked polyubiquitin chains on AGO2,and facilitated AGO2 degradation through ubiquitin-proteasome system.In addition to AGO2,STUB1 also induced the protein degradation of AGO1,AGO3 and AGO4.Further investigation revealed that STUB1 also regulated Dicer's ubiquitination via K48-linked polyubiquitin and induced the degradation of Dicer as well as its specialized form,termed antiviral Dicer(avi Dicer)that expresses in mammalian stem cells.Moreover,we found that STUB1 deficiency up-regulated Dicer and AGO2,thereby enhancing the RNAi response and efficiently inhibiting viral replication in mammalian cells.Using the newborn mouse model of Enterovirus A71(EV-A71),we confirmed that STUB1 deficiency enhanced the virus-derived si RNAs production and antiviral RNAi,which elicited a potent antiviral effect against EV-A71 infection in vivo.In summary,our findings uncovered that the E3 ubiquitin ligase STUB1 was a general regulator of the RNAi machinery by targeting Dicer,avi Dicer and AGO1–4.Moreover,STUB1 regulated the RNAi response through mediating the abundance of Dicer and AGO2 during viral infection,thereby providing novel insights into the regulation of antiviral RNAi in mammals.展开更多
Constructing noble-metal-free loaded catalyst with high-efficiency photocatalytic activity by a simple and scalable method is of profound significance for fundamental research and practical application.Herein,a simple...Constructing noble-metal-free loaded catalyst with high-efficiency photocatalytic activity by a simple and scalable method is of profound significance for fundamental research and practical application.Herein,a simple one-pot method was used to synthesize novel samples of array-like sulfur-doped graphitic carbon nitride(SCN)nanosheets with ultrathin MoS2 loading(MS/SCN-x%).The ultrathin MoS2 cocatalyst was evenly distributed on the surface of SCN and was linked to the main catalyst by covalent chemical bonds.Benefited from the multiple advantages of the array-like porous nanosheets structure with rich exposed surface,covalent cross-linking structure,and enhanced visible light absorption,the MS/SCN-2.5%composites drastically improve hydrogen evolution performance,which is superior to original MoS2 nanosheet modified by two-step mixing method,and also rivals with Pt/SCN.The designing strategy of photocatalyst modified by noble-metal-free cocatalyst with covalent bond structure provides fascinating insights into enhanced photocatalytic hydrogen evolution.展开更多
文摘Objective: To observe the analgesic effect of acupuncture and moxibustion on chronic constriction injury (CCI) pain model rats, and to investigate the mechanism of constriction constriction injury. Methods: 32 SPF SD rats were divided into Control Group, Sham Surgery Group, Model Group and Acupuncture and moxibustion Group, with 8 rats in each group. The Model Group, Acupuncture and moxibustion Group and sciatic nerve ligation were used to establish the CCI pain rat model. The sham operation group only separated the nerve, and the control group did not do any treatment. After modeling, the Acupuncture and moxibustion Group was given acupuncture treatment, while the control group and the Model Group did not do any intervention. 7 days for 1 course of treatment, continuous treatment for 3 courses of sampling and detection. Paw withdrawal mechanical threshold (PWMT) and Paw withdrawal thermal latency (PWTL) were observed. Biochemical detection of reactive oxygen species (ROS), malondialdehyde (MDA), Super Oxide Dismutase (SOD), glutathione (GSH);Serum, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels were detected by ELISA. Results: Compared with the control group, PWMT and PWTL in the Model Group were decreased (P α, IL-1β and IL-6 levels or contents were increased (P α, IL-1β and IL-6 levels or contents were decreased (P Conclusion: Acupuncture and moxibustion can reduce the ROS level, enhance the antioxidant capacity of the body, reduce the damage of sciatic nerve caused by inflammation, and relieve sciatic pain.
文摘Hydrogen peroxide(H_(2)O_(2))has gained widespread attention as a versatile oxidant and a mild disin-fectant.Here,an electrostatic self-assembly method is applied to couple ZnSe quantum dots(QDs)with a flower-like covalent organic framework(COF)to form a step-scheme(S-scheme)photocata-lyst for H_(2)O_(2)production.The as-prepared S-scheme photocatalyst exhibits a broad light absorption range with an edge at 810 nm owing to the synergistic effect between the ZnSe QDs and COF.The S-scheme charge-carrier transfer mechanism is validated by performing Fermi level calculations and in-situ X-ray photoelectron and femtosecond transient absorption spectroscopies.Photolumi-nescence,time-resolved photoluminescence,photocurrent response,electrochemical impedance spectroscopy,and electron paramagnetic resonance results show that the S-scheme heterojunction not only promotes charge carrier separation but also boosts the redox ability,resulting in enhanced photocatalytic performance.Remarkably,a 10%-ZnSe QD/COF has excellent photocatalytic H_(2)O_(2)-production activity,and the optimal S-scheme composite with ethanol as the hole scavenger yields a H_(2)O_(2)-production rate of 1895 mol g^(-1)h^(-1).This study presents an example of a high-performance organic/inorganic S-scheme photocatalyst for H_(2)O_(2)production.
基金supported the by Anhui Provincial Natural Science Foundation under Grant 2308085MF223in part by the Open Fund of State Key Laboratory for Novel Software Technology under Grant KFKT2022B33+1 种基金in part by the by the Foundation of Yunnan Key Laboratory of Service Computing under Grant YNSC23106in part by the Key Project on Anhui Provincial Natural Science Study by Colleges and Universities under Grant 2023AH050495,2024AH051078 and Grant KJ2020A0513.
文摘Advancements in the vehicular network technology enable real-time interconnection,data sharing,and intelligent cooperative driving among vehicles.However,malicious vehicles providing illegal and incorrect information can compromise the interests of vehicle users.Trust mechanisms serve as an effective solution to this issue.In recent years,many researchers have incorporated blockchain technology to manage and incentivize vehicle nodes,incurring significant overhead and storage requirements due to the frequent ingress and egress of vehicles within the area.In this paper,we propose a distributed vehicular network scheme based on trust scores.Specifically,the designed architecture partitions multiple vehicle regions into clusters.Then,cloud supervision systems(CSSs)verify the accuracy of the information transmitted by vehicles.Additionally,the trust scores for vehicles are calculated to reward or penalize them based on the trust evaluation model.Our proposed scheme demonstrates good scalability and effectively addresses the main cause of malicious information distribution among vehicles.Both theoretical and experimental analysis show that our scheme outperforms the compared schemes.
基金supported by the Jilin Province Science and Technology Department Program(YDZJ202201ZYTS304)the Science and Technology Project of Jilin Provincial Education Department(JJKH20220428KJ)+3 种基金the Jilin Province Science and Technology Department Program(YDZJ202101ZYTS047)the National Natural Science Foundation of China(21905110,21905041,22279045,22102020)the Special foundation of Jilin Province Industrial Technology Research and Development(2019C042)the Fundamental Research Funds for the Central Universities(2412020FZ008)。
文摘Lithium sulfur batteries are regarded as a promising candidate for high-energy-density energy storage devices.However,the lithium metal anode in lithium-sulfur batteries encounters the problem of lithium dendrites and lithium metal consumption caused by polysulfide corrosion.Herein we design a dualfunction PMMA/PPC/LiNO3composite as an artificial solid electrolyte interphase(PMCN-SEI)to protect Li metal anode.This SEI offers multiple sites of C=O for polysulfide anchoring to constrain corrosion of Li metal anode.The lithiated polymer group and Li3N in PMCN-SEI can homogenize lithium-ion deposition behavior to achieve a dendrite-free anode.As a result,the PMCN-SEI protected Li metal anode enables the Li||Li symmetric batteries to maintain over 300 cycles(1300 h)at a capacity of 5 m Ah cm^(-2),corresponding to a cumulative capacity of 3.25 Ah cm^(-2).Moreover,Li-S batteries assembled with 20μm of Li metal anode(N/P=1.67)still deliver an initial capacity of 1166 m A h g-1at 0.5C.Hence,introducing polycarbonate polymer/inorganic composite SEI on Li provides a new solution for achieving the high energy density of Li-S batteries.
基金supported by National Natural Science Foundation of China[No.41401663]Natural Science Foundation of Shandong Province[No.2010ZRE14029]+1 种基金China Social Science University Humanity Foundation[No.11YJC790283 and No.12YJC790254]Excellent Young Scholars Research Fund of Shandong Normal University
文摘Wetland is one of the most important ecosystems with varied functions and structures,and its loss has been a major issue.Wetland loss in Modem Yellow River Delta(MYRD) becomes a serious environmental problem,so its restoration attracts a great deal of attention from academia and governments.This article proposes a GIS-based multi-criteria comprehensive evaluation methodology for potential estimation of wetland restoration,using MYRD as an example.The model uses four kinds of data(hydrology,terrain,soil,and land use) and could be adapted by planners for use in identifying the suitability of locations as wetland mitigation sites at any site or region.In the application of the model in the MYRD,the research developed a lost wetland distributed map taking the better wetland situation of 1995 as the reference,and elevated the overall distribution trends of wetland restoration potential based on wetland polygon.The results indicated that the total area of wetland loss from 1995 to 2014 was 568.12 km^2,which includes 188.83 km^2 natural wetland and 21.80 km^2 artificial wetland,respectively.The areas of lost wetland with low,middle,and high resilience ability are 126.82 km^2,259.92 km^2,and 119.59 km^2,occupying 25.05%,51.33%,and 23.62%,respectively.The high-restoration-potential wetland included98.47 km^2 of natural wetland and 21.12 km^2 of artificial wetland,which are mainly bush,reed,and ponds.The highrestoration-potential wetland is mainly distributed in the vicinity of Gudong oil field,the Yellow River Delta protected areas,and the eastern sides of Kenli county and Dongying city.
文摘Prostate mucinous adenocarcinoma with signet ring cells (MC-SRC) is a rare morphologic variant of prostate cancer, with only 12 cases reported to date. Diagnosis of this carcinoma requires that at least 25% of the tumor tissue should consist of an extracellular mucin pool. In this report, we present a case of prostate prostate mucinous adenocarcinoma with signet ring cells.
文摘Background: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damage. At the same time, some studies have proved that Zhuangtongy in (ZTY) has a certain protective effect on the myocardium of rats with coronary heart disease. Therefore, the aim of this study is to observe and explore the effects of ZTY on cardiomyocytes and its mechanism of action in rats with coronary heart disease based on the Caspase-1 mediated cellular pyroptosis pathway. Methods: Fifty SD rats were randomly divided into blank group, sham-operation group, model group, ZTY group and cysteinyl aspartate specific protease (Caspase-1) inhibitor group. The blank group did not receive any treatment, and the other groups established a rat model of coronary artery disease by ligating the left anterior descending branch of the coronary artery, but the sham-operation group was only threaded without ligation. After that, the sham-operated and model groups were gavaged with 0.1 ml/kg distilled water, the ZTY group was gavaged with 13.6 g/kg ZTY decoction based on the previous study, and the Caspase-1 inhibitor group was injected intraperitoneally with 3 mg/kg Ac-YVAD-cmk solvent once a day for four consecutive weeks. Transmission electron microscopy was used to observe the ultrastructure of cardiomyocytes, HE staining was used to observe the morphology of cardiac tissue, and enzyme-linked immunosorbent assay (Elisa) was used to detect serum interleukin-1β (IL-1β), interleukin-18 (IL-18) and C-reactive protein (CRP) in the rats. In addition, fluorescent probe was used to detect serum Reactive oxygen species (ROS). Real-time fluorescence quantitative PCR was used to detect the expression of mRNAs of ASC, Nlrp3, Caspase-1 and gasdermin-D (GSDMD), and the protein expression of Caspase-1 and GSDMD was detected by immunohistochemistry. Results: There was no significant difference between the results of the blank and sham-operated groups. Compared with the blank group, the transmission electron microscopy results showed swollen and ruptured cardiomyocyte membranes in the model group, with pore formation, severe mitochondrial swelling, membrane lysis, and cristae breakage. The staining results showed myofibril breakage, severe intercellular oedema and vacuolation in the model group. The inflammatory factors IL-1β, IL-18, CRP and ROS were significantly elevated (P β, IL-18, CRP, ROS and the expression of ASC, Nlrp3, Caspase-1, and GSDMD mRNA were significantly elevated (P Conclusion: ZTY can reduce myocardial injury by lowering the expression of ROS and other inflammatory factors, inhibiting Caspase-1 mediated cellular pyroptosis, thus reducing myocardial inflammation and protecting cardiomyocytes.
基金grateful for the financial support from the National Natural Science Foundation of China(51875486 and 52122507)Sichuan Science and Technology Program(2021YFSY0017)the Fundamental Research Funds for the Central Universities(2682021ZTPY095).
文摘Achievement of steady and reliable super-low friction at the steel/steel contact interface,one of the most tribological systems applied for mechanical moving parts,is of importance for prolonging machine lifetime and reducing energy consumption.Here we reported that the superlubricity performance of the steel/steel sliding interface lubricated with tiny amounts of diketone solution strongly depends on the oxygen content in surrounding environment.The increase of oxygen not only significantly shortens the initial running-in time but also further reduces the stable coefficient of friction in superlubricity stage due to the enhancement of tribochemical reactions.On the one hand,more severe oxidation wear occurring at higher oxygen content facilitates material removal of the contact interface,lowering the contact pressure and the corresponding initial friction.On the other hand,the growth of iron ions during the shear process in high oxygen environment promotes the formation of chelate which acted as an effective lubricated film chemisorbed at the steel/steel friction interface to further lower the interfacial friction.The results provide a new opportunity to further optimize the tribological performance of diketone superlubricity system,especially towards the lubrication of mechanical engineering materials.
基金the National Natural Science Foundation of China(31930106,31829004 and 31722054)the 2115 Talent Development Program of China Agricultural University(1041-00109019)+3 种基金the China National Postdoctoral Program for Innovation Talents(BX20200365)China Postdoctoral Science Foundation(2020M680771)the National Ten-thousand Talents Program of China(23070201)the project of Academician Workstation in Chengdejiuyun Agricultural and livestock Co.,Ltd(199A7310H).
文摘The offspring of super-multiparous sows face problems such as decreased growth performance,poor meat quality and even diseases in animal husbandry.Gama-aminobutyric acid(GABA)has long been known to promote growth and suppress inflammation,but little is known about the mechanisms.A total of 72 growing-finishing pigs from the 8th generation were randomly allotted to 2 groups with 6 replicates per treatment to receive a cornesoybean basal diet or the basal diet supplemented 20 mg/kg GABA for 60 d.After the animal-trial period,samples of serum and liver were collected for further analysis.Additionally,a lipopolysaccharide(LPS)-induced inflammatory model using HepG2 cells was established to explore the role of GABA on regulating hepatic inflammation.The results indicated that inflammatory cell infiltration occurs in the liver of progeny of super-multiparous sows,and dietary supplementation with GABA influenced liver morphology,increased activities of antioxidant enzymes and decreased the expression abundance of pro-inflammatory cytokines,including tumor necrosis factor-α(TNFα)and interleukin(IL)-1β,in the liver of growing-finishing pigs(P<0.05).In addition,GABA supplementation increased mRNA expressions of peroxisome proliferator-activated receptor g(PPARg)and GABA receptors(GABARs),and reduced the expression of toll-like receptor 4(TLR4)/nuclear factor-kB(NF-kB)signaling(P<0.05).Additionally,an in vitro experiment demonstrated that GABA decreased the expressions of hepatic TLR4/NF-kB signaling via activating GABARs under LPS-stress(P<0.05).In summary,liver injury may affect the growth performance of growing-finishing pigs by changing hepatic mitochondrial metabolism,the expression of pro-inflammatory cytokines and TLR4/NF-kB pathway and that GABA supplementation has a restorative effect by acting on GABARs.
文摘Dear Editor,Taisui(太岁) has attracted great attention in China because it has been described in ancient Chinese texts as an entity possessing mysterious properties. In Chinese mythology and folklore, Jupiter is the God of the "Sui"(year) and is worshiped in Daoism. Jupiter appears at the same location in the sky approximately every 12 years(Jupiter’s orbit is11.86 years). However, since each cycle of Jupiter lasts for exactly 11.86 years and one Chinese year cycle comprises exactly 12 years, after approximately 84.7 years, Jupiter travels one cycle more than the number of cycles assumed by the ancient Chinese, which is called Taisui. According to the belief of the ancient Chinese, people are alerted when Taisui is digging in the ground, foretelling the destruction of houses or occurrence of natural disasters, such as floods and earthquakes(Zhao et al., 2018).
基金the National Natural Science Foundation of China(31970169 to X.Z.and 82172269 and 81873964 to Y.Q.)the International Partnership Program of Chinese Academy of Sciences(153B42KYSB20200004 to X.Z.)+3 种基金the Young Top-notch Talent Cultivation Program of Hubei Province(Y.Q.)the Grant from the CAS Youth Innovation Promotion Association(2020332 to Y.Q.)the Hubei Province Natural Science Funds for Distinguished Young Scholar(2021CFA047 to Y.Q.)the Young Top-notch Talent Cultivation Program of Hubei Province(Y.Q.)。
文摘RNA interference(RNAi)is an intrinsic antiviral immune mechanism conserved in diverse eukaryotic organisms.However,the mechanism by which antiviral RNAi in mammals is regulated is poorly understood.In this study,we uncovered that the E3 ubiquitin ligase STIP1 homology and U-box-containing protein 1(STUB1)was a new regulator of the RNAi machinery in mammals.We found that STUB1 interacted with and ubiquitinated AGO2,and targeted it for degradation in a chaperon-dependent manner.STUB1 promoted the formation of Lys48(K48)-linked polyubiquitin chains on AGO2,and facilitated AGO2 degradation through ubiquitin-proteasome system.In addition to AGO2,STUB1 also induced the protein degradation of AGO1,AGO3 and AGO4.Further investigation revealed that STUB1 also regulated Dicer's ubiquitination via K48-linked polyubiquitin and induced the degradation of Dicer as well as its specialized form,termed antiviral Dicer(avi Dicer)that expresses in mammalian stem cells.Moreover,we found that STUB1 deficiency up-regulated Dicer and AGO2,thereby enhancing the RNAi response and efficiently inhibiting viral replication in mammalian cells.Using the newborn mouse model of Enterovirus A71(EV-A71),we confirmed that STUB1 deficiency enhanced the virus-derived si RNAs production and antiviral RNAi,which elicited a potent antiviral effect against EV-A71 infection in vivo.In summary,our findings uncovered that the E3 ubiquitin ligase STUB1 was a general regulator of the RNAi machinery by targeting Dicer,avi Dicer and AGO1–4.Moreover,STUB1 regulated the RNAi response through mediating the abundance of Dicer and AGO2 during viral infection,thereby providing novel insights into the regulation of antiviral RNAi in mammals.
基金supported by the National Natural Science Foundation of China(51872087)the National Natural Science Foundation of China(21871030)+2 种基金the Research Foundation of Education Bureau of Hunan Provincial(19A053,19B062)the Hunan Provincial Natural Science Foundation of China(2018JJ2456,2020JJ4644)the Hunan Provincial Natural Science Foundation of China(14JJ5010)。
文摘Constructing noble-metal-free loaded catalyst with high-efficiency photocatalytic activity by a simple and scalable method is of profound significance for fundamental research and practical application.Herein,a simple one-pot method was used to synthesize novel samples of array-like sulfur-doped graphitic carbon nitride(SCN)nanosheets with ultrathin MoS2 loading(MS/SCN-x%).The ultrathin MoS2 cocatalyst was evenly distributed on the surface of SCN and was linked to the main catalyst by covalent chemical bonds.Benefited from the multiple advantages of the array-like porous nanosheets structure with rich exposed surface,covalent cross-linking structure,and enhanced visible light absorption,the MS/SCN-2.5%composites drastically improve hydrogen evolution performance,which is superior to original MoS2 nanosheet modified by two-step mixing method,and also rivals with Pt/SCN.The designing strategy of photocatalyst modified by noble-metal-free cocatalyst with covalent bond structure provides fascinating insights into enhanced photocatalytic hydrogen evolution.
