Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detectio...Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared （NIR） regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region （termed ＂NIR-IIb＂） under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region.展开更多
Glioblastoma(GBM)is the most common malignant primary tumor in the central nervous system.Despite advances in neurosurgery,radiation therapy and chemotherapy,the median survival time of GBM patients is only 9 to 16 mo...Glioblastoma(GBM)is the most common malignant primary tumor in the central nervous system.Despite advances in neurosurgery,radiation therapy and chemotherapy,the median survival time of GBM patients is only 9 to 16 months.1 Therefore,GBM is considered one of the deadliest human cancers.In the past two decades,many efforts have been made by scientists and clinicians to develop new drugs to improve current therapies.Unfortunately,most efforts have not achieved long-term remissions in clinical trials,even though some of them are promising in animal models,making treatment options still limited.展开更多
文摘Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared （NIR） regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region （termed ＂NIR-IIb＂） under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region.
基金supported by NSFC81302187,CWS14C063 and SIMM1705KF-10(State Key Laboratory of Drug Research).
文摘Glioblastoma(GBM)is the most common malignant primary tumor in the central nervous system.Despite advances in neurosurgery,radiation therapy and chemotherapy,the median survival time of GBM patients is only 9 to 16 months.1 Therefore,GBM is considered one of the deadliest human cancers.In the past two decades,many efforts have been made by scientists and clinicians to develop new drugs to improve current therapies.Unfortunately,most efforts have not achieved long-term remissions in clinical trials,even though some of them are promising in animal models,making treatment options still limited.
