Effects of different processing methods on the quality of Polygonatum cyrtonema Hua

Background:In this study,we explored the effects of different processing methods on the quality of Polygonatum cyrtonema Hua(PC),and the role of Huangjiu in the processing procedure.Methods:The sensory characteristics of the crude product,steamed product,and wine-processed product of PC were described.The colorimeter was used to analyze the chromatic values of three different processed products on PC.At the same time,the contents of the water extract and alcohol extract were measured separately.The content of three different processing Polygonatum Polysaccharide(PCP)was determined using 0.2%anthrone-sulfuric acid.The correlation difference between the chromatic values and chemical composition of different PC products was analyzed using various analytical methods.Results:The surface colors gradually deepened,the sweetness increased,the viscosity strengthened,and the tongue-numbing sensation disappeared after PC processing.The contents of extract and L^(*) gradually decreased from the crude to the steamed to the wine-processed product,consistent with the pattern of surface color alteration.While,E^(*)ab gradually increased.The content of PCP was crude product>wine-processed product>steamed product.The results of multivariate statistical analysis showed that the samples processed for crude,steamed,and wine-processed product were clustered into three classes.The correlation analysis showed that L^(*)and E^(*)ab were highly significant positively correlated with the content of PCP,and a*was significantly negatively correlated with the content of PCP.Conclusion:The results showed that the wine-processed product had the best quality.The internal quality of the PC was correlated with its characteristics and chromatic value.In this study,we investigated the internal and external quality of three different products of PC in order to provide a reference for further research on the impact of different processing methods on PC quality,the standardization of PC processing,and the role of Huangjiu in the processing of PC.

Study on the regulatory effect of liver X receptor in HEK293 cells by six main diterpene esters in Semen Euphorbiae

Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.

Study on the processing technology of Polygonatum cyrtonema Hua decoction pieces based on softening method and drying method

Background:To optimize the steaming processing technology of Polygonatum cyrtonema Hua(PC)decoction pieces.Methods:The softening method and drying method of PC decoction pieces were studied with Polygonatum Polysaccharide(PCP),diosgenin combined extract as quantitative indexes.The process parameters such as softening time,drying temperature and drying time were determined,and the best processing technology of PC decoction pieces was optimized.Results:Among the three softening methods of PC,the infiltration method had the highest ranking,with an average comprehensive index of 0.9496,and the softening effect was the best.Among the three drying methods,the drying effect of the hot air drying method was the best,and the average comprehensive index was 0.8233.Conclusion:The infiltration method is the best softening method for PC decoction pieces,and the hot air drying method is the best drying method for PC decoction pieces.

Exploring the mechanism of Huangjing pill in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking

Objective To reveal the mechanism of Huangjing pill in treating Alzheimer’s disease(AD)based on network pharmacology and molecular docking technology.Methods We obtained the active ingredients and targets of Huangjing pill through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,and supplemented the effective components by consulting literature and predicted targets through the PharmMapper database.We used DrugBank,the GeneCards,the TTD,and the OMIM database to collect targets of AD.The Venn diagram was drawn and the key targets of Huangjing pill in the treatment of AD were obtained by Venny 2.1 platform.The Cytoscape 3.8.1 software was used to construct a network diagram of“drugs-active ingredients-key targets-disease”.The protein-protein interaction(PPI)network diagram was constructed through the STRING 11.5 database.DAVID database was used for Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis.AutoDock Vina1.1.2 software was used for molecular docking of the active components and core targets,and PyMOL 1.7.2.1 software was used for visual processing.Results After screening,we obtained 13 active ingredients and 116 targets of Huangjing Pill,1438 related targets for AD,and 75 common targets.566 items by GO enrichment analysis and 149 items related to KEGG pathway enrichment were obtained.Molecular docking results showed that there is a strong affinity between the key active ingredients and the core targets.Conclusion This study revealed that Huangjing pill could treat AD through multiple components,multiple targets and multiple pathways.

Autologous scalp skin grafting to treat toxic epidermal necrolysis in a patient with a large skin injury:A case report

BACKGROUND Toxic epidermal necrolysis(TEN)is often associated with skin wounds affecting large areas.Healing of this type of wound is difficult because of pressure,infection and other factors.It can increase the length of hospital stay and result in wound sepsis and even death.CASE SUMMARY A 49-year-old woman developed a skin lesion covering 80%of the total body surface area after using a kind of Chinese medicinal ointment on a burn wound on her back;she developed life-threatening wound sepsis and septic shock.Methicillin-resistant Staphylococcus aureus,carbapenem-resistant Acinetobacter baumannii,carbapenem-resistant Pseudomonas aeruginosa and other bacteria were cultured from wound tissue,deep venous catheter and blood samples.Imipenem cilastatin sodium,tigecycline and teicoplanin were used for anti-infection therapy.Finally,the patient was transferred to the burn department because of severe wound sepsis.In the burn intensive care unit,pain-free dressing changes and autologous scalp skin grafting were performed to heal the wound in addition to reasonable and effective antibacterial treatment according to microbial susceptibility test results.After three operations within 2 wk,the wound healed and sepsis resolved.CONCLUSION TEN patients with large areas of skin injury may develop wound infection and life-threatening wound sepsis.Autologous scalp skin grafting may be beneficial for rapid wound healing and reducing the risk of sepsis in TEN patients,and it leaves no scar at the donor site.

Differential distribution of authenticity results of different varieties of refined honey based on stable isotope ratio method

Objective Using the stable isotope ratio method for the authenticity identification and variety identification of refined honey.Methods In this paper,a total of 17 samples of different varieties of refined honey were used to obtain refined honey proteins by precipitation with sodium tungstate solution and sulphuric acid solution.The isotope mass spectrometer was used to simultaneously detect theδ^(13)C values of refined honey proteins and refined honey as well as theδ^(18)O andδ^(2)H values of refined honey,processed of the results obtained,analysed the authenticity of the samples and conduct a variety identification study.Results Tested of the resulting honey samples,the results showed that four batches of refined honey did not up to standard,two batches of C-4 vegetable syrup were detected as adulterated,and two batches of protein were not detected.Theδ^(18)O andδ^(2)H values of refined honey were also found to be effective in distinguishing the varietal origin of refined honey to a certain extent.Conclusions The stable isotope ratio method is useful in the authenticity identification of refined honey,and provides new ideas to further promote the authenticity of refined honey and variety identification research.

Effect of Toosendan Fructus before and after stir-frying process extract on CYP450 enzyme activities in rats in vivo and vitro by cocktail probe drug method

Background:The traditional Chinese medicine Toosendan Fructus has certain hepatotoxicity,which is used after being processed by stir-frying to attenuate toxicity.However,there are few studies on its attenuating toxicity mechanism.The effects of Toosendan Fructus on the activities of CYP450P1A2,CYP2E1 and CYP3A4 were studied in vivo and in vitro and the dose-toxicity mechanism of hepatotoxicity before and after stir-frying was explored to provide the basis for safe,rational use of Toosendan Fructus.Methods:The rat liver microsomes in vitro incubation method and in vivo pharmacokinetics were used to detect the concentrations of phenacetin,chlorzoxazone and dapsone in the liver microsomes in vitro incubation system and the rat plasma to study the effect of stir-frying of Toosendan Fructus on the activity of CYP450P1A2,CYP2E1,CYP3A4.Results:The results of pharmacokinetics in vivo showed that the AUC of phenacetin and dapsone in different groups was lower,and CL value was higher than those of the normal group.At the same dose,the AUC of stir-fried Toosendan Fructus was higher than that of the raw,while CL value was lower.For the same processed product,AUC value was high-dose>low-dose>middle-dose group,CL value was high-dose<low-dose<middle-dose.AUC and CL values of chlorzoxazone showed no difference from those of the normal group.The results of pharmacokinetics in vivo showed that Toosendan Fructus can induce the activity of CYP3A4 in a dose-dependent manner and the induction effect will decrease after stir-frying in vitro.Conclusion:The toxicity attenuation of Toosendan Fructus may be related to the decrease of induction effect after stir-frying.These results would provide the basis for safe,rational use of Toosendan Fructus.

Readout system for ground-based tests of BGO calorimeter of DAMPE satellite

A readout system for ground-based tests of the bismuth germanium oxide(BGO) calorimeter of the Dark Matter Particle Explorer satellite is described in this paper.The system mainly consists of a data acquisition board with a field-programmable gate array to implement the control logic, and a graphical user interface software based on LabWindows/CVI. The system has been successfully applied in a series of ground-based environmental experiments and almost all the performance tests throughout the entire manufacturing processes. These contribute significantly to the development of the BGO calorimeter before being submitted for satellite-level integration.

Investigation on 1-Heptene/Air Laminar Flame Propagation under Elevated Pressures

The laminar flame propagation of 1-heptene/air mixtures covering equivalence ratios from 0.7 to 1.5 is investigated in a constant-volume cylindrical combustion vessel at 373K and elevated pressures (1, 2, 5, and 10 atm). Laminar flame speed and Markstein length are derived from the recorded schlieren images. A kinetic model of 1-heptene combustion is developed based on our previous kinetic model of 1-hexene. The model is validated against the laminar flame speed data measured in this work and the ignition delay time data in literature. Modeling analyses, such as sensitivity analysis and rate of production analysis, are performed to help understand the high temperature chemistry of 1-heptene under various pressures and its influence on the laminar flame propagation. Furthermore, the laminar flame propagation of 1-heptene/air mixtures is compared with that of n-heptane/air mixtures reported in our previous work. The laminar flame speed values of 1-heptene/air mixtures are observed to be faster than those of n-heptane/air mixtures under most conditions due to the enhanced exothermicity and reactivity.

Secondary injuries caused by ill-suited rehabilitation treatments: Five case reports

BACKGROUND Increasing attention is being given to burn rehabilitation,and an increasing number of burn patients are undergoing rehabilitation.However,little attention has been given to secondary injuries caused by ill-suited rehabilitation practices.Patients,especially those in undeveloped countries,sustain injuries due to inadequate burn rehabilitation practices and the nonstandard implementation of rehabilitation treatments.CASE SUMMARY This study reports 5 cases of secondary injuries caused by ill-suited burn rehabilitation practices in our institute,including first web space contracture caused by a single orthosis treatment,finger deformity caused by improper compression therapy with a self-adhering bandage,developmental impairment of the affected limb caused by continuous improper compression therapy,and fracture caused by overly intensive rehabilitation exercise.CONCLUSION More attention should be given to burn rehabilitation to reduce the incidence of secondary injuries caused by ill-suited rehabilitation practices.Burn rehabilitation system should include specialized hospitals(burn rehabilitation centers),community hospitals,and family rehabilitation clinics.Precise instruments and equipment,accurate measurement methods,objective monitoring indicators and standardized guidelines and recommendations will help improve the quality of burn rehabilitation.Additionally,more attention should be given to burn rehabilitation in children.

Tumor necrosis factor receptor-associated protein 1 regulates hypoxia-induced apoptosis through a mitochondria-dependent pathway mediated by cytochrome c oxidase subunit Ⅱ

Background:Tumor necrosis factor receptor-associated protein 1(TRAP1)plays a protective effect in hypoxic cardiomyocytes,but the precise mechanisms are not well clarified.The study is aimed to identify the mechanism of TRAP1 on hypoxic damage in cardiomyocytes.Methods:In this study,the effects of TRAP1 and cytochrome c oxidase subunit Ⅱ(COXⅡ)on apoptosis in hypoxia-induced cardiomyocytes were explored using overexpression and knockdown methods separately.Results:Hypoxia induced cardiomyocyte apoptosis,and TRAP1 overexpression notably inhibited apoptosis induced by hypoxia.Conversely,TRAP1 silencing promoted apoptosis in hypoxic cardiomyocytes.Further investigation revealed that the proapoptotic effects caused by the silencing of TRAP1 were prevented by COXⅡ overexpression,whereas COXⅡ knockdown reduced the antiapoptotic function induced by TRAP1 overexpression.Additionally,changes in the release of cytochrome c from mitochondria into the cytosol and the caspase-3 activity in the cytoplasm,as well as reactive oxygen species production,were found to be correlated with the changes in apoptosis.Conclusions:The current study uncovered that TRAP1 regulates hypoxia-induced cardiomyocyte apoptosis through a mitochondria-dependent apoptotic pathway mediated by COXⅡ,in which reactive oxygen species presents as an important component.