Erratum to:Bioinformatic analysis for potential biological processes and key targets of heart failure-related stroke

Erratum to:J Zhejiang Univ-Sci B(Biomed&Biotechnol)202122(9):718-732 https:/doi.org/10.1631/jzus.B2000544.The original version of this article(Liu et al.,2021)unfortunately contained a mistake:statement of equal contribution is missing.This correction article shows that Chiyu LIU and Sixu CHEN contributed equally to this work.The original article has been corrected.

Bioinformatic analysis for potential biological processes and key targets of heart failure-related stroke

This study aimed to uncover underlying mechanisms and promising intervention targets of heart failure(HF)-related stroke.HF-related dataset GSE42955 and stroke-related dataset GSE58294 were obtained from the Gene Expression Omnibus(GEO)database.Weighted gene co-expression network analysis(WGCNA)was conducted to identify key modules and hub genes.Gene Ontology(GO)and pathway enrichment analyses were performed on genes in the key modules.Genes in HF-and stroke-related key modules were intersected to obtain common genes for HF-related stroke,which were further intersected with hub genes of stroke-related key modules to obtain key genes in HF-related stroke.Key genes were functionally annotated through GO in the Reactome and Cytoscape databases.Finally,key genes were validated in these two datasets and other datasets.HF-and stroke-related datasets each identified two key modules.Functional enrichment analysis indicated that protein ubiquitination,Wnt signaling,and exosomes were involved in both HF-and stroke-related key modules.Additionally,ten hub genes were identified in stroke-related key modules and 155 genes were identified as common genes in HF-related stroke.OTU deubiquitinase with linear linkage specificity(OTULIN)and nuclear factor interleukin 3-regulated(NFIL3)were determined to be the key genes in HF-related stroke.Through functional annotation,OTULIN was involved in protein ubiquitination and Wnt signaling,and NFIL3 was involved in DNA binding and transcription.Importantly,OTULIN and NFIL3 were also validated to be differentially expressed in all HF and stroke groups.Protein ubiquitination,Wnt signaling,and exosomes were involved in HF-related stroke.OTULIN and NFIL3 may play a key role in HF-related stroke through regulating these processes,and thus serve as promising intervention targets.

平均夜间基线阻抗对食管外症状胃食管反流病的临床诊断价值

目的探讨食管平均夜间基线阻抗(MNBI)对表现为食管外症状的胃食管反流病(GERD)的临床诊断价值。方法回顾性分析2018年1月至2019年12月期间以烧心、反流、咳嗽或声音嘶哑等为主要症状就诊于首都医科大学附属北京朝阳医院,并行24 h多通路腔内pH-阻抗监测的210例患者。按照诊断及分组标准将纳入患者分为四组,GERD食管外症状组39例、GERD典型症状组64例、GERD混合症状组52例及对照组55例。分析并比较各组间食管远端及近端平均夜间基线阻抗及其它反流参数。结果GERD食管外症状组的近端食管MNBI值、食管下段平均pH值低于典型症状组(P<0.05)。相关性分析显示近端及远端食管MNBI值与DeMeester积分、酸暴露时间和酸反流事件次数呈显著负相关(P<0.05)。食管外症状的GERD患者,近端食管MNBI值的诊断曲线下面积显著大于远端食管MNBI值的诊断曲线下面积(P<0.05),最佳诊断临界值为2484.38Ω,诊断敏感性及准确性分别达92.3%和84%,高于远端食管MNBI值的诊断敏感性及准确性。结论近端及远端食管MNBI值均与食管酸暴露相关。近端食管MNBI值可提高多通路pH-阻抗监测对食管外症状的GERD患者的检出率,具有重要的临床诊断价值。