目的:采用双硫死亡相关基因构建的风险评分模型在肿瘤免疫景观和预测预后方面具有出色的能力。但缺乏在肺腺癌中的研究报道。本研究探讨双硫死亡相关基因在肺腺癌中的意义及其对患者预后的影响。方法:从肿瘤与癌症基因组图谱(The Cancer...目的:采用双硫死亡相关基因构建的风险评分模型在肿瘤免疫景观和预测预后方面具有出色的能力。但缺乏在肺腺癌中的研究报道。本研究探讨双硫死亡相关基因在肺腺癌中的意义及其对患者预后的影响。方法:从肿瘤与癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载正常肺和肺腺癌样本的基因表达谱及临床信息。使用Deseq2包分析差异基因,采用Cox回归分析差异基因与相关性。利用Timer数据库进行靶基因泛癌分析,按靶基因表达中位数将患者分为高表达组与低表达组,分析靶基因表达与临床特征及预后的关系。通过癌症影像档案馆(The Cancer Imaging Archive,TCIA)数据库分析靶基因和免疫相关性。最后进行临床样本免疫组织化学表达和治疗效果分析。结果:识别出预后相关的双硫死亡相关基因溶质载体家族成员SLC7A11(solute carrier family 7 member 11)、CD2AP(CD2 associated protein)和辅肌动蛋白α4(actinin alpha 4,ACTN4),其中ACTN4的风险比最高。ACTN4在泛癌种中广泛表达,在肺腺癌中显著降低,可以显著区分不同预后的患者。ACTN4的表达与临床分期和免疫检查点表达水平显著相关。临床样本的免疫组织化学结果表明肺腺癌中ACTN4高表达患者对免疫治疗的反应效果较好。结论:双硫死亡相关基因ACTN4在肺腺癌中发挥重要作用,有望为肺腺癌患者的治疗提供一定的参考。展开更多
Aragonite is a metastable mineral,which is easily transformed into calcite,and generally difficult to preserve in the stratum.However,large amounts of aragonites were found in the Paleogene shale of the Jiyang Depress...Aragonite is a metastable mineral,which is easily transformed into calcite,and generally difficult to preserve in the stratum.However,large amounts of aragonites were found in the Paleogene shale of the Jiyang Depression.The characteristics and preservation mechanisms of these aragonites were analyzed through a series of analytical methods,including cathodoluminescence,field-emission scanning electron microscopy(FESEM),laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS),microarea carbon and oxygen isotopes,Sr isotopes,and dissolution simulation experiments under high temperature and high pressure.The research results show that:(1)Aragonite in the Paleogene shale of the Jiyang Depression is related to algal microbial fossils,primarily composed of coccoliths and characterized by two emission peaks at 420 nm and 480 nm in cathodoluminescence;(2)The primary factor allowing biological aragonite to be preserved is the immaturity of the organic matter and the deficiency of abundant organic acids necessary for its dissolution or transformation,which is confirmed by the evidence of organic matter maturity and simulation experiments of organic acid dissolution on aragonite under high-temperature and high-pressure conditions.Additional factors that may aid in the preservation of aragonite are the ideal sedimentation conditions,the defense of organic coating,and the enclosed environment with tiny pores,low porosity,and low permeability;(3)These aragonite-rich shales,characterized by coccolithophores,provide a solid evidence for seawater intrusion into terrestrial lake basin,and have a significant implication for the source and storage of shale oil.展开更多
文摘目的:采用双硫死亡相关基因构建的风险评分模型在肿瘤免疫景观和预测预后方面具有出色的能力。但缺乏在肺腺癌中的研究报道。本研究探讨双硫死亡相关基因在肺腺癌中的意义及其对患者预后的影响。方法:从肿瘤与癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载正常肺和肺腺癌样本的基因表达谱及临床信息。使用Deseq2包分析差异基因,采用Cox回归分析差异基因与相关性。利用Timer数据库进行靶基因泛癌分析,按靶基因表达中位数将患者分为高表达组与低表达组,分析靶基因表达与临床特征及预后的关系。通过癌症影像档案馆(The Cancer Imaging Archive,TCIA)数据库分析靶基因和免疫相关性。最后进行临床样本免疫组织化学表达和治疗效果分析。结果:识别出预后相关的双硫死亡相关基因溶质载体家族成员SLC7A11(solute carrier family 7 member 11)、CD2AP(CD2 associated protein)和辅肌动蛋白α4(actinin alpha 4,ACTN4),其中ACTN4的风险比最高。ACTN4在泛癌种中广泛表达,在肺腺癌中显著降低,可以显著区分不同预后的患者。ACTN4的表达与临床分期和免疫检查点表达水平显著相关。临床样本的免疫组织化学结果表明肺腺癌中ACTN4高表达患者对免疫治疗的反应效果较好。结论:双硫死亡相关基因ACTN4在肺腺癌中发挥重要作用,有望为肺腺癌患者的治疗提供一定的参考。
基金supported by the National Natural Science Foundation of China(Nos.42302152,42072164,41821002,and 42272119)the Natural Science Foundation of Shandong Province(No.ZR2023QD076)+3 种基金the Shandong Provincial Key research and Development Program(No.2020ZLYS08)the Taishan Scholars Program(No.TSQN201812030)the Fundamental Research Funds for the Central Universities(No.22CX06010A)Qingdao Postdoctoral Application Research Project(No.QDBSH20220202075)。
文摘Aragonite is a metastable mineral,which is easily transformed into calcite,and generally difficult to preserve in the stratum.However,large amounts of aragonites were found in the Paleogene shale of the Jiyang Depression.The characteristics and preservation mechanisms of these aragonites were analyzed through a series of analytical methods,including cathodoluminescence,field-emission scanning electron microscopy(FESEM),laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS),microarea carbon and oxygen isotopes,Sr isotopes,and dissolution simulation experiments under high temperature and high pressure.The research results show that:(1)Aragonite in the Paleogene shale of the Jiyang Depression is related to algal microbial fossils,primarily composed of coccoliths and characterized by two emission peaks at 420 nm and 480 nm in cathodoluminescence;(2)The primary factor allowing biological aragonite to be preserved is the immaturity of the organic matter and the deficiency of abundant organic acids necessary for its dissolution or transformation,which is confirmed by the evidence of organic matter maturity and simulation experiments of organic acid dissolution on aragonite under high-temperature and high-pressure conditions.Additional factors that may aid in the preservation of aragonite are the ideal sedimentation conditions,the defense of organic coating,and the enclosed environment with tiny pores,low porosity,and low permeability;(3)These aragonite-rich shales,characterized by coccolithophores,provide a solid evidence for seawater intrusion into terrestrial lake basin,and have a significant implication for the source and storage of shale oil.
