In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 T...In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 Trichoderma strains on preventing cucumber fusarium wilt through pot culture and greenhouse culture experiments. All 3 Trichoderma strains demonstrated higher control effects toward cucumber fusarium wilt than previous studies, with efficacies over 78%. Additionally, inoculation with the 3 Trichoderma strains significantly promoted the quality and yield of cucumbers. Among the 3 strains, Trichoderma 866 was the most effective, with disease control efficacy of 78.64% and a cucumber yield increase of 33%. Furthermore, seedlings inoculated with Trichoderma exhibited significantly increased measures of plant height, stem diameter, leaf area, aboveground fresh weight, underground fresh weight, chlorophyll content, and nitric nitrogen content, as well as the activities of several stress-resistance enzymes, including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO), and ascorbate oxidase(AAO). In addition, the plants inoculated with Trichoderma showed decreased cell membrane permeability and malondialdehyde(MDA) content in the leaves. Together, our results suggest that T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886 inoculations inhibit F. oxysporum infection, stimulate the metabolism in cucumbers, and enhance the activities of stress-resistance enzymes, which consequently promote the growth of cucumber plants, prevent cucumber fusarium wilt, and improve the yield and quality of cucumbers. T. harzianum is a commonly used biocontrol fungus, while few studies have focused on T. asperellum or T. koningense. In this study, strains of T. asperellum and T. pseudokoningii showed excellent plant disease prevention and growth promoting effects on cucumber, indicating that they also have great potential as biocontrol fungi.展开更多
目的将躯体症状、情感症状及负性事件三者结合,编制一份适合在综合医院实施的躯体形式障碍筛查量表并进一步验证其信度、效度。方法通过查阅文献、参考中国精神疾病分类中躯体形式障碍的诊断标准(第3版)(Chinese Classification of Ment...目的将躯体症状、情感症状及负性事件三者结合,编制一份适合在综合医院实施的躯体形式障碍筛查量表并进一步验证其信度、效度。方法通过查阅文献、参考中国精神疾病分类中躯体形式障碍的诊断标准(第3版)(Chinese Classification of Mental Disorders,third edition,CCMD-3)、国际疾病分类(International Classification of Diseases,ICD)-10躯体形式障碍诊断标准,结合临床经验构建躯体形式障碍三维度量表模型,包括躯体症状、负性情感、负性事件3项条目,并进行预调查条目分析和验证预问卷模型,形成躯体形式障碍筛查量表(简称Neuro-11)。对2014年6月-2016年6月就诊于深圳市人民医院神经内科的461例患者进行基本资料、躯体形式障碍筛查量表、广泛性焦虑量表(theseven-item generalized anxiety disorder scale,GAD-7)、9项患者健康问卷(patient health questionnaire-9,PHQ-9)的调查及测评,精神科医师根据ICD-10躯体形式障碍诊断标准进行电话访谈诊断,诊断结果为躯体形式障碍诊断的金标准。分析各人口学特点的得分情况,通过ROC曲线分析该量表的临界值,分析量表的内部信度及效标效度,分析量表3个维度之间的关系。结果 Neuro-11包含3个维度及11个条目,3个维度分别为躯体症状、负性情感及负性事件。当量表得分≥10分时,可认为患者有躯体形式障碍倾向,所对应的敏感度为0.88,特异度为0.72。Neuro-11的内部一致性Cronbach α系数为0.73,分半信度系数为0.68;并且随着Neuro-11的得分升高,GAD-7、PHQ-9的得分也升高。结论综合医院躯体形式障碍筛查量表的三维理论合理,对躯体形式障碍的诊断价值良好,具有良好的信度、效度,并且可作为躯体形式障碍严重程度的衡量工具。展开更多
目的探讨脑小血管病(cerebral small vessel disease,CSVD)患者运动功能及影像特征与经颅磁刺激运动诱发电位(transcranial magnetic stimulation induced motor evoked potentials,TMS-MEP)的相关性。方法回顾性分析深圳市人民医院神...目的探讨脑小血管病(cerebral small vessel disease,CSVD)患者运动功能及影像特征与经颅磁刺激运动诱发电位(transcranial magnetic stimulation induced motor evoked potentials,TMS-MEP)的相关性。方法回顾性分析深圳市人民医院神经内科2018年1月-2019年8月住院的符合CSVD诊断标准且已行TMS-MEP检查的患者。记录患者的性别、年龄和血管危险因素等临床资料及MEP相关电生理数据,包括中枢运动传导时间(central motor conduction time,CMCT)和静息运动阈值(resting motor threshold,RMT)。评估并量化CSVD的影像学特征,包括近期皮质下小梗死(recent small subcortical infarct,RSSI)、腔隙、扩大的血管周围间隙、脑白质高信号、脑微出血和脑萎缩。根据是否有肌力减退分为症状半球组和非症状半球组。比较两组间的影像学特征及MEP特征,分析CSVD影像特征与MEP的相关性。结果研究共纳入202例CSVD患者,共404侧大脑半球。平均年龄64.0±10.6岁,男性135例(66.8%)。症状半球组77侧(19.1%),非症状半球组327侧(80.9%)。症状半球组中CMCT(P=0.005)和RMT(P<0.001)高于非症状半球组。症状半球组RSSI数目多于非症状半球组(P<0.001)。RSSI与CMCT(r=0.512,P=0.043)及RMT(r=0.730,P<0.001)正相关。脑白质高信号、腔隙、扩大的血管周围间隙、微出血及脑萎缩与CMCT、RMT均无相关性。结论 RSSI与CMCT、RMT正相关,RSSI可能是导致CSVD相关运动障碍的主要影像学特征。展开更多
In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave p...In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave power, initial moisture content, puffing time and mass were employed as experimental factors. The pressure, temperature, amount of water vapor and puffing properties in terms of the volume, hardness and brittleness were employed as target parameters. The higher microwave intensity and initial moisture content resulted in the more amount of water vapor in the snacks and more holes in the appropriate puffing time. The shape and structure of the snacks tended to be stable with the removal of moisture.展开更多
基金support from the National Key R&D Program of China (2018YFD0201202)the National Science and Technology Basic Work, China (2014FY120900)the 948 Program of China (2011-G4)
文摘In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 Trichoderma strains on preventing cucumber fusarium wilt through pot culture and greenhouse culture experiments. All 3 Trichoderma strains demonstrated higher control effects toward cucumber fusarium wilt than previous studies, with efficacies over 78%. Additionally, inoculation with the 3 Trichoderma strains significantly promoted the quality and yield of cucumbers. Among the 3 strains, Trichoderma 866 was the most effective, with disease control efficacy of 78.64% and a cucumber yield increase of 33%. Furthermore, seedlings inoculated with Trichoderma exhibited significantly increased measures of plant height, stem diameter, leaf area, aboveground fresh weight, underground fresh weight, chlorophyll content, and nitric nitrogen content, as well as the activities of several stress-resistance enzymes, including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO), and ascorbate oxidase(AAO). In addition, the plants inoculated with Trichoderma showed decreased cell membrane permeability and malondialdehyde(MDA) content in the leaves. Together, our results suggest that T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886 inoculations inhibit F. oxysporum infection, stimulate the metabolism in cucumbers, and enhance the activities of stress-resistance enzymes, which consequently promote the growth of cucumber plants, prevent cucumber fusarium wilt, and improve the yield and quality of cucumbers. T. harzianum is a commonly used biocontrol fungus, while few studies have focused on T. asperellum or T. koningense. In this study, strains of T. asperellum and T. pseudokoningii showed excellent plant disease prevention and growth promoting effects on cucumber, indicating that they also have great potential as biocontrol fungi.
文摘目的探讨脑小血管病(cerebral small vessel disease,CSVD)患者运动功能及影像特征与经颅磁刺激运动诱发电位(transcranial magnetic stimulation induced motor evoked potentials,TMS-MEP)的相关性。方法回顾性分析深圳市人民医院神经内科2018年1月-2019年8月住院的符合CSVD诊断标准且已行TMS-MEP检查的患者。记录患者的性别、年龄和血管危险因素等临床资料及MEP相关电生理数据,包括中枢运动传导时间(central motor conduction time,CMCT)和静息运动阈值(resting motor threshold,RMT)。评估并量化CSVD的影像学特征,包括近期皮质下小梗死(recent small subcortical infarct,RSSI)、腔隙、扩大的血管周围间隙、脑白质高信号、脑微出血和脑萎缩。根据是否有肌力减退分为症状半球组和非症状半球组。比较两组间的影像学特征及MEP特征,分析CSVD影像特征与MEP的相关性。结果研究共纳入202例CSVD患者,共404侧大脑半球。平均年龄64.0±10.6岁,男性135例(66.8%)。症状半球组77侧(19.1%),非症状半球组327侧(80.9%)。症状半球组中CMCT(P=0.005)和RMT(P<0.001)高于非症状半球组。症状半球组RSSI数目多于非症状半球组(P<0.001)。RSSI与CMCT(r=0.512,P=0.043)及RMT(r=0.730,P<0.001)正相关。脑白质高信号、腔隙、扩大的血管周围间隙、微出血及脑萎缩与CMCT、RMT均无相关性。结论 RSSI与CMCT、RMT正相关,RSSI可能是导致CSVD相关运动障碍的主要影像学特征。
基金Supported by National Natural Science Foundation of China(31571848)
文摘In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave power, initial moisture content, puffing time and mass were employed as experimental factors. The pressure, temperature, amount of water vapor and puffing properties in terms of the volume, hardness and brittleness were employed as target parameters. The higher microwave intensity and initial moisture content resulted in the more amount of water vapor in the snacks and more holes in the appropriate puffing time. The shape and structure of the snacks tended to be stable with the removal of moisture.
