Time-gated(TG)fluorescence imaging(TGFI)has attracted increasing attention within the biological imaging community,especially during the past decade.With rapid development of light sources,image devices,and a variety ...Time-gated(TG)fluorescence imaging(TGFI)has attracted increasing attention within the biological imaging community,especially during the past decade.With rapid development of light sources,image devices,and a variety of approaches for TG implementation,TGFI has demonstrated numerous biological applications ranging from molecules to tissues.The paper presents inclusive TG implementation mainly based on optical choppers and electronic units for synchronization of fluorescence excitation and emission,which also serves as guidelines for researchers to build suited TGFI systems for selected applications.Note that a special focus will be put on TG implementation based on optical choppers for TGFI of long-lived probes(lifetime range from microseconds to milliseconds).Biological applications by TG imaging of recently developed luminescent probes are described.展开更多
Photoacoustic(PA)imaging has been widely used in biomedical research and preclinical studies during the past two decades.It has also been explored for nondestructive testing and evaluation(NDT/E)and for industrial app...Photoacoustic(PA)imaging has been widely used in biomedical research and preclinical studies during the past two decades.It has also been explored for nondestructive testing and evaluation(NDT/E)and for industrial applications.This paper describes the basic principles of PA technology for NDT/E and its applications in recent years.PA technology for NDT/E includes the use of a modulated continuous-wave laser and a pulsed laser for PA wave excitation,PA-generated ultrasonic waves,and all-optical PA wave excitation and detection.PA technology for NDT/E has demonstrated broad applications,including the imaging of railway cracks and defects,the imaging of Li metal batteries,the measurements of the porosity and Young’s modulus,the detection of defects and damage in silicon wafers,and a visualization of underdrawings in paintings.展开更多
Following publication of the original article[1],the authors identified an error in the article title.The first word ‘Review’ is added mistakenly by the typesetter.
Photoacoustic imaging(PAI) is an emerging technology that has been dramatically developed in the last decade. PAI, a combination of optical illumination and ultrasound detection, allows us to achieve fine resolution a...Photoacoustic imaging(PAI) is an emerging technology that has been dramatically developed in the last decade. PAI, a combination of optical illumination and ultrasound detection, allows us to achieve fine resolution and obtain fruitful information of endogenous and exogenous chromophores. Among PAI imaging techniques, photoacoustic computed tomography(PACT)has been extensively used in human studies due to its deep tissue penetration(several centimeters). Alternatively, photoacoustic microscopy(PAM) offers higher resolution at the expense of penetration depth, which can also be advantageous in clinics.Recently, there has been increasing attention and studies on PAM of human tissues. In this paper, we will review principles of PAM and its applications to human tissues, including the breast tissue, carotid atheroma tissue, eye, gastrointestinal tissue,ovarian tissue and tooth ex vivo as well as the oral cavity and dermatological tissue in vivo. The paper is closed with the outlook regarding the potential applications of PAM in clinics.展开更多
基金supported by National Natural Science Foundation(NSFC)(Grant No.61775134)
文摘Time-gated(TG)fluorescence imaging(TGFI)has attracted increasing attention within the biological imaging community,especially during the past decade.With rapid development of light sources,image devices,and a variety of approaches for TG implementation,TGFI has demonstrated numerous biological applications ranging from molecules to tissues.The paper presents inclusive TG implementation mainly based on optical choppers and electronic units for synchronization of fluorescence excitation and emission,which also serves as guidelines for researchers to build suited TGFI systems for selected applications.Note that a special focus will be put on TG implementation based on optical choppers for TGFI of long-lived probes(lifetime range from microseconds to milliseconds).Biological applications by TG imaging of recently developed luminescent probes are described.
基金S.-L.Chen acknowledges funding from the National Natural Science Foundation of China,No.61775134C.Tian acknowledges funding from the National Natural Science Foundation of China,No.61705216the Anhui Science and Technology Department,No.18030801138.
文摘Photoacoustic(PA)imaging has been widely used in biomedical research and preclinical studies during the past two decades.It has also been explored for nondestructive testing and evaluation(NDT/E)and for industrial applications.This paper describes the basic principles of PA technology for NDT/E and its applications in recent years.PA technology for NDT/E includes the use of a modulated continuous-wave laser and a pulsed laser for PA wave excitation,PA-generated ultrasonic waves,and all-optical PA wave excitation and detection.PA technology for NDT/E has demonstrated broad applications,including the imaging of railway cracks and defects,the imaging of Li metal batteries,the measurements of the porosity and Young’s modulus,the detection of defects and damage in silicon wafers,and a visualization of underdrawings in paintings.
文摘Following publication of the original article[1],the authors identified an error in the article title.The first word ‘Review’ is added mistakenly by the typesetter.
基金supported by the National Natural Science Foundation of China(61775134)。
文摘Photoacoustic imaging(PAI) is an emerging technology that has been dramatically developed in the last decade. PAI, a combination of optical illumination and ultrasound detection, allows us to achieve fine resolution and obtain fruitful information of endogenous and exogenous chromophores. Among PAI imaging techniques, photoacoustic computed tomography(PACT)has been extensively used in human studies due to its deep tissue penetration(several centimeters). Alternatively, photoacoustic microscopy(PAM) offers higher resolution at the expense of penetration depth, which can also be advantageous in clinics.Recently, there has been increasing attention and studies on PAM of human tissues. In this paper, we will review principles of PAM and its applications to human tissues, including the breast tissue, carotid atheroma tissue, eye, gastrointestinal tissue,ovarian tissue and tooth ex vivo as well as the oral cavity and dermatological tissue in vivo. The paper is closed with the outlook regarding the potential applications of PAM in clinics.
