Objective:Tribulus terrestris saponin is a traditional Chinese medicine in China.This experiment was designed to investigate the effects of tribulus terrestris saponin on the proliferation and invasion ability of non-...Objective:Tribulus terrestris saponin is a traditional Chinese medicine in China.This experiment was designed to investigate the effects of tribulus terrestris saponin on the proliferation and invasion ability of non-small cell lung cancer A549 cells.Methods:A549 cells were divided into normal control and experimental groups(Tribulus terrestris saponin 250μg/mL group,Tribulus terrestris saponin 200μg/mL group,Tribulus terrestris saponin 150μg/mL group,Tribulus terrestris saponin 100μg/mL group,Tribulus terrestris saponin 50μg/mL group).The proliferation viability of the cells in each group was detected by CCK8,the invasion of tumor cells was detected by Transwell model.The mRNA expression of MMP9 and caspase-3 in each group of cells was detected by RT-PCR.Immunofluorescence staining was used to observe the fluorescence intensity of caspase-3 in each group of cells.Results:Compared with the normal control group,tribulus terrestris saponin significantly inhibited the proliferation activity and invasion ability of A549 cells,which was statistically significant(P<0.01).In the invasion assay,compared with the control group,MMP9 expression was significantly reduced and caspase-3 expression was significantly increased in the tribulus terrestris saponin group,and both were concentration-dependent,with statistically significant differences(P<0.01).By cellular immunofluorescence staining experiments,it was found that the fluorescence expression of caspase-3 was enhanced in the experimental group compared with the normal control group,in which the high concentration saponin group was significantly higher than the low concentration group.Conclusion:Tribulus terrestris saponin can inhibit the invasive ability of A549 cells by down-regulating the expression of MMP9,and induce irreversible apoptosis by up-regulating the activation of caspase-3 expression to form caspase-3.展开更多
目的研究比较纳米银和纳米锌2种材料的抑菌效果和对虾的保鲜效果。方法以G^+和G^-为受试菌种,采用纸片扩散法研究纳米银和纳米锌的高、中、低浓度对细菌的抑菌效果。在此基础上,以总挥发性盐基氮(total volatile base nitrogen,TVBN)、...目的研究比较纳米银和纳米锌2种材料的抑菌效果和对虾的保鲜效果。方法以G^+和G^-为受试菌种,采用纸片扩散法研究纳米银和纳米锌的高、中、低浓度对细菌的抑菌效果。在此基础上,以总挥发性盐基氮(total volatile base nitrogen,TVBN)、菌落总数、感官、色度等为指标,研究其应用于南美白对虾4℃贮藏保鲜效果。结果纳米银对受试菌种的抑菌效果明显优于纳米锌;纳米银对G^+的抑菌效果优于对G^-的抑菌效果;以TVBN限值为指标,纳米银处理组较对照组在4℃下保存时间可延长48 h。结论纳米银可作为虾类保鲜材料开发的抑菌增效剂。展开更多
基金National Key R&D Plan(2022YFC2305004)Hainan Province Major Science and Technology Special Project(No.ZDKJ2021036)+3 种基金Key R&D projects in Hainan Province(No.ZDYF2020223)Hainan Province Key R&D Plan International Science and Technology Cooperation Project(GHYF2022011)Hainan Provincial Innovation Team Project(No.820CXTD448)National Natural Science Foundation of China(No.82260001,82160012)。
文摘Objective:Tribulus terrestris saponin is a traditional Chinese medicine in China.This experiment was designed to investigate the effects of tribulus terrestris saponin on the proliferation and invasion ability of non-small cell lung cancer A549 cells.Methods:A549 cells were divided into normal control and experimental groups(Tribulus terrestris saponin 250μg/mL group,Tribulus terrestris saponin 200μg/mL group,Tribulus terrestris saponin 150μg/mL group,Tribulus terrestris saponin 100μg/mL group,Tribulus terrestris saponin 50μg/mL group).The proliferation viability of the cells in each group was detected by CCK8,the invasion of tumor cells was detected by Transwell model.The mRNA expression of MMP9 and caspase-3 in each group of cells was detected by RT-PCR.Immunofluorescence staining was used to observe the fluorescence intensity of caspase-3 in each group of cells.Results:Compared with the normal control group,tribulus terrestris saponin significantly inhibited the proliferation activity and invasion ability of A549 cells,which was statistically significant(P<0.01).In the invasion assay,compared with the control group,MMP9 expression was significantly reduced and caspase-3 expression was significantly increased in the tribulus terrestris saponin group,and both were concentration-dependent,with statistically significant differences(P<0.01).By cellular immunofluorescence staining experiments,it was found that the fluorescence expression of caspase-3 was enhanced in the experimental group compared with the normal control group,in which the high concentration saponin group was significantly higher than the low concentration group.Conclusion:Tribulus terrestris saponin can inhibit the invasive ability of A549 cells by down-regulating the expression of MMP9,and induce irreversible apoptosis by up-regulating the activation of caspase-3 expression to form caspase-3.
文摘目的研究比较纳米银和纳米锌2种材料的抑菌效果和对虾的保鲜效果。方法以G^+和G^-为受试菌种,采用纸片扩散法研究纳米银和纳米锌的高、中、低浓度对细菌的抑菌效果。在此基础上,以总挥发性盐基氮(total volatile base nitrogen,TVBN)、菌落总数、感官、色度等为指标,研究其应用于南美白对虾4℃贮藏保鲜效果。结果纳米银对受试菌种的抑菌效果明显优于纳米锌;纳米银对G^+的抑菌效果优于对G^-的抑菌效果;以TVBN限值为指标,纳米银处理组较对照组在4℃下保存时间可延长48 h。结论纳米银可作为虾类保鲜材料开发的抑菌增效剂。
