期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
C-kit gene mutation in human gastrointestinal stromal tumors 被引量:3
1
作者 Ying-YongHou Yun-ShanTan +12 位作者 Meng-HongSun Yong-KunWei Jian-FangXu Shao-HuaLu Su-JieA-Ke-Su Yan-NanZhou FengGao Ai-HuaZheng tai-mingzhang Wen-ZhongHou JianWang XiangDu Xiong-ZengZhu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第9期1310-1314,共5页
AIM: TO investigate the significance of c-kit gene mutation in gastrointestinal stromal tumors (GIST).METHODS: Fifty two cases of GIST and 28 cases of other tumors were examined. DNA samples were extracted from paraff... AIM: TO investigate the significance of c-kit gene mutation in gastrointestinal stromal tumors (GIST).METHODS: Fifty two cases of GIST and 28 cases of other tumors were examined. DNA samples were extracted from paraffin sections and fresh blocks. Exons 11, 9 and 13 of the c-kit gene were amplified by PCR and sequenced.RESULTS: Mutations of exon 11 were found in 14 of 25 malignant GISTs (56%), mutations of exon 11 of the c-kit gene were revealed in 2 of 19 borderline GISTs (10.5%),and no mutation was found in benign tumors. The mutation rate showed significant difference (x^2=14.39, P<0.01) between malignant and benign GISTs. Most of mutations consisted of the in-frame deletion or replication from 3 to 48 bp in heterozygous and homozygous fashions, None of the mutations disrupted the downstream reading frame of the gene. Point mutations and frame deletions were most frequently observed at codons 550-560, but duplications were most concentrated at codons 570-585. No mutations of exons 9 and 13 were revealed in GISTs, Neither c-kit gene expression nor gene mutations were found in 3 leiomyomas, 8 leiomyosarcomas, 2 schwannomas, 2 malignant peripheral nerve sheath tumors, 2 intra-abdominal fibromatoses, 2 malignant fibrous histiocytomas and 9 adenocarcinomas.CONCLUSION: C-kit gene mutations occur preferentially in malignant GISTs and might be a clinically useful adjunct marker in the evaluation of GISTs and can help to differentiate GISTs from other mesenchymal tumors of gastrointestinal tract, such as smooth muscle tumors,schwannomas, etc. 展开更多
关键词 C-KIT基因 胃肠基质癌 基因突变 肿瘤病理学 DNA扩增
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部