Because of the continuous improvement of the quality of life,the population of gout patients is also increasing,which seriously affects the quality of life of patients.Traditional Chinese medicine compound has a long ...Because of the continuous improvement of the quality of life,the population of gout patients is also increasing,which seriously affects the quality of life of patients.Traditional Chinese medicine compound has a long history and remarkable clinical effect in treating gout patients,and has been widely used.Intestinal flora and its metabolites are the focus of current research,which can promote intestinal mucosal barrier,improve immunity and improve metabolism.The regulation of intestinal flora can reduce serum uric acid and inhibit inflammation to fight gouty arthritis.Moreover,the mechanism of intestinal flora is related to the spleen in Chinese medicine theory.Therefore,this study discusses the research mechanism and clinical application of traditional Chinese medicine compound in treating gout by regulating intestinal flora,and provides theoretical basis for the clinical application of traditional Chinese medicine and the expansion of new dosage forms.展开更多
Objective:To explore the potential mechanism of hepatotoxicity induced by Aconitum brachypodum through network toxicology.Methods:The active components and targets of Aconitum brachypodum were identified and screened ...Objective:To explore the potential mechanism of hepatotoxicity induced by Aconitum brachypodum through network toxicology.Methods:The active components and targets of Aconitum brachypodum were identified and screened by CNKI,PubChem database,Swiss Target Prediction database.Genecards,pharmGKB and DisGeNET databases were used to collect hepatotoxicity related targets.The intersection targets were obtained by matching the active component targets with the hepatotoxic targets of Aconitum brachypodum.Cytoscape software was used to construct the"Aconitum brachypodum-potential active components-potential targets-hepatotoxicity"network.The STRING database was used to construct the protein-protein interaction(PPI)network of the targets and to screen out the core targets.In addition,Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted by R software.The toxic components in Aconitum brachypodum were docked with the core targets.Results:In this study,26 chemical components were screened via SwissADME,297 targets for the active components of Aconitum brachypodum were obtained.There were 1,096 hepatotoxicity-related targets,73 potential targets for hepatotoxicity caused by Aconitum brachypodum,and 15 potential active components,among which Penduline,Songoramine,Sitosterol,Daucosterol and Bullatine A were the key active components for hepatotoxicity caused by Aconitum brachypodum,and signal transducer and activator of transcription 3(STAT3),epidermal growth factor receptor(EGFR),mitogen-activated protein kinase 8(MAPK8)and tyrosine-protein kinase JAK2(JAK2)were the potential targets for hepatotoxicity caused by Aconitum brachypodum.There were 1,133 GO entries(P<0.05),including 1,045 entries of biological process(BP),19 entries of cellular component(CC),and 69 entries of molecular function(MF).KEGG enrichment analysis revealed 115 pathways(P<0.05),of which EGFR tyrosine kinase inhibitor resistance,hypoxia-inducible factor 1(HIF-1)signaling pathway,PI3K-Akt signaling pathway,calcium signaling pathway,T helper 17(Th17)cell differentiation was strongly correlated with the hepatotoxicity caused by Aconitum brachypodum.Molecular docking results showed that the binding activity was good.Conclusion:Through network toxicology analysis,it was found that the active ingredients in Aconitum brachypodum may act on multiple targets and signaling pathways,thereby participating in the activation of an excessive inflammatory response,oxidative stress,apoptosis and other pathways on the whole,thus resulting in hepatotoxicity.展开更多
Objective:To explore the effect of moxibustion for preventing post-hemorrhoidectomy urinary retention of mixed hemorrhoids.Design:Two-arm randomized clinical trial with blinded outcome assessment and statistician.Sett...Objective:To explore the effect of moxibustion for preventing post-hemorrhoidectomy urinary retention of mixed hemorrhoids.Design:Two-arm randomized clinical trial with blinded outcome assessment and statistician.Setting:Anorectal Department of Changshu Affiliated Hospital of Nanjing University of Chinese Medicine,from October 2020 to a cutoff day in March 2021.Participants:120 patients with mixed hemorrhoids undertaken hemorrhoidectomy.Interventions:In the moxibustion group,moxibustion was exerted after surgery at Zhongji(CV3),Qihai(CV6),Guanyuan(CV4)and Sanyinjiao(SP6,bilateral).In the medication group,tamsulosin was administered orally after surgery.The cases were all observed for 24 h in two groups.Outcome measures:The primary outcome was incidence of post-hemorrhoidectomy urinary retention and the secondary outcomes included time to the first voiding,the first voiding volume and adverse events were measured and compared between two groups.Results:Sixty patients in each group were included in the data analysis and no change was made to the design of the trial after the trial begun.After treatment,the incidence of the post-hemorrhoidectomy urinary retention was 5%in the moxibustion group,lower than 20%in the medication group(P<0.05).The time to the first voiding after surgery was(4.76±1.69)h in the moxibustion group,shorter than(6.81±1.15)h in the medication group(P<0.05).The first voiding volume in the moxibustion group was(300.67±110.33)mL,higher than(224.67±90.88)mL in the medication group(P<0.05).There was no adverse event during study in the moxibustion group.Dizziness and nausea occurred in the medication group.Conclusion:Moxibustion reduced the incidence of post-hemorrhoidectomy urinary retention,shortened the time to the first voiding and improved the urine volume after hemorrhoidectomy of mixed hemorroids.This therapy could be beneficial and safe,and thus could be recommended to the clinical practice.展开更多
文摘Because of the continuous improvement of the quality of life,the population of gout patients is also increasing,which seriously affects the quality of life of patients.Traditional Chinese medicine compound has a long history and remarkable clinical effect in treating gout patients,and has been widely used.Intestinal flora and its metabolites are the focus of current research,which can promote intestinal mucosal barrier,improve immunity and improve metabolism.The regulation of intestinal flora can reduce serum uric acid and inhibit inflammation to fight gouty arthritis.Moreover,the mechanism of intestinal flora is related to the spleen in Chinese medicine theory.Therefore,this study discusses the research mechanism and clinical application of traditional Chinese medicine compound in treating gout by regulating intestinal flora,and provides theoretical basis for the clinical application of traditional Chinese medicine and the expansion of new dosage forms.
文摘Objective:To explore the potential mechanism of hepatotoxicity induced by Aconitum brachypodum through network toxicology.Methods:The active components and targets of Aconitum brachypodum were identified and screened by CNKI,PubChem database,Swiss Target Prediction database.Genecards,pharmGKB and DisGeNET databases were used to collect hepatotoxicity related targets.The intersection targets were obtained by matching the active component targets with the hepatotoxic targets of Aconitum brachypodum.Cytoscape software was used to construct the"Aconitum brachypodum-potential active components-potential targets-hepatotoxicity"network.The STRING database was used to construct the protein-protein interaction(PPI)network of the targets and to screen out the core targets.In addition,Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted by R software.The toxic components in Aconitum brachypodum were docked with the core targets.Results:In this study,26 chemical components were screened via SwissADME,297 targets for the active components of Aconitum brachypodum were obtained.There were 1,096 hepatotoxicity-related targets,73 potential targets for hepatotoxicity caused by Aconitum brachypodum,and 15 potential active components,among which Penduline,Songoramine,Sitosterol,Daucosterol and Bullatine A were the key active components for hepatotoxicity caused by Aconitum brachypodum,and signal transducer and activator of transcription 3(STAT3),epidermal growth factor receptor(EGFR),mitogen-activated protein kinase 8(MAPK8)and tyrosine-protein kinase JAK2(JAK2)were the potential targets for hepatotoxicity caused by Aconitum brachypodum.There were 1,133 GO entries(P<0.05),including 1,045 entries of biological process(BP),19 entries of cellular component(CC),and 69 entries of molecular function(MF).KEGG enrichment analysis revealed 115 pathways(P<0.05),of which EGFR tyrosine kinase inhibitor resistance,hypoxia-inducible factor 1(HIF-1)signaling pathway,PI3K-Akt signaling pathway,calcium signaling pathway,T helper 17(Th17)cell differentiation was strongly correlated with the hepatotoxicity caused by Aconitum brachypodum.Molecular docking results showed that the binding activity was good.Conclusion:Through network toxicology analysis,it was found that the active ingredients in Aconitum brachypodum may act on multiple targets and signaling pathways,thereby participating in the activation of an excessive inflammatory response,oxidative stress,apoptosis and other pathways on the whole,thus resulting in hepatotoxicity.
基金Supported by Suzhou Special Project of the Clinical Diagnosis and Treatment Technology for Major Diseases:LCZX202022Science and Technology Planning Project of Changshu Municipal Health Commission:CSWSQ202108+1 种基金supported by Changshu Health Commission Science and Technology Plan Project(CSWSQ202108)Suzhou ClinicalKey Disease Diagnosis and Treatment Technology Special Project(LCZX202022)。
文摘Objective:To explore the effect of moxibustion for preventing post-hemorrhoidectomy urinary retention of mixed hemorrhoids.Design:Two-arm randomized clinical trial with blinded outcome assessment and statistician.Setting:Anorectal Department of Changshu Affiliated Hospital of Nanjing University of Chinese Medicine,from October 2020 to a cutoff day in March 2021.Participants:120 patients with mixed hemorrhoids undertaken hemorrhoidectomy.Interventions:In the moxibustion group,moxibustion was exerted after surgery at Zhongji(CV3),Qihai(CV6),Guanyuan(CV4)and Sanyinjiao(SP6,bilateral).In the medication group,tamsulosin was administered orally after surgery.The cases were all observed for 24 h in two groups.Outcome measures:The primary outcome was incidence of post-hemorrhoidectomy urinary retention and the secondary outcomes included time to the first voiding,the first voiding volume and adverse events were measured and compared between two groups.Results:Sixty patients in each group were included in the data analysis and no change was made to the design of the trial after the trial begun.After treatment,the incidence of the post-hemorrhoidectomy urinary retention was 5%in the moxibustion group,lower than 20%in the medication group(P<0.05).The time to the first voiding after surgery was(4.76±1.69)h in the moxibustion group,shorter than(6.81±1.15)h in the medication group(P<0.05).The first voiding volume in the moxibustion group was(300.67±110.33)mL,higher than(224.67±90.88)mL in the medication group(P<0.05).There was no adverse event during study in the moxibustion group.Dizziness and nausea occurred in the medication group.Conclusion:Moxibustion reduced the incidence of post-hemorrhoidectomy urinary retention,shortened the time to the first voiding and improved the urine volume after hemorrhoidectomy of mixed hemorroids.This therapy could be beneficial and safe,and thus could be recommended to the clinical practice.