Fast-neutron (FN) irradiation has been used to create mutagen- ized collections of many plant species (Bolon et al., 2014), FN- induced mutagenesis has clear advantages: it is an efficient means of saturating the...Fast-neutron (FN) irradiation has been used to create mutagen- ized collections of many plant species (Bolon et al., 2014), FN- induced mutagenesis has clear advantages: it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis (Wang et al., 2013). Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.展开更多
文摘Fast-neutron (FN) irradiation has been used to create mutagen- ized collections of many plant species (Bolon et al., 2014), FN- induced mutagenesis has clear advantages: it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis (Wang et al., 2013). Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.