采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术对杀螺剂胁迫下福寿螺肝脏蛋白质组进行研究,以期从蛋白质组学水平了解福寿螺对杀螺剂四聚乙醛的胁迫应答。结果鉴定到108个具有定量信...采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术对杀螺剂胁迫下福寿螺肝脏蛋白质组进行研究,以期从蛋白质组学水平了解福寿螺对杀螺剂四聚乙醛的胁迫应答。结果鉴定到108个具有定量信息的差异蛋白(P<0.05),其中显著上调的蛋白有55个,下调蛋白53个。理化性质分析和GO(gene ontology)注释分析发现,这些蛋白分别具有抗氧化、催化结合、抗菌等功能,并影响福寿螺的细胞结构组成、肌肉收缩、代谢调节和免疫调节,引起神经毒性。研究测定出57个具有不同活性的细胞色素450酶系蛋白(CYP450),推测CYP450活性是福寿螺对四聚乙醛产生抗药性的重要原因。展开更多
The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and co...The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.展开更多
The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 15...The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.展开更多
文摘采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术对杀螺剂胁迫下福寿螺肝脏蛋白质组进行研究,以期从蛋白质组学水平了解福寿螺对杀螺剂四聚乙醛的胁迫应答。结果鉴定到108个具有定量信息的差异蛋白(P<0.05),其中显著上调的蛋白有55个,下调蛋白53个。理化性质分析和GO(gene ontology)注释分析发现,这些蛋白分别具有抗氧化、催化结合、抗菌等功能,并影响福寿螺的细胞结构组成、肌肉收缩、代谢调节和免疫调节,引起神经毒性。研究测定出57个具有不同活性的细胞色素450酶系蛋白(CYP450),推测CYP450活性是福寿螺对四聚乙醛产生抗药性的重要原因。
基金This study was supported by grants from the International Found for Sciences(No.A/3224-1)Cooperative Project of the Key Lab of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture(No.LFB20040503)+1 种基金the National Natural Science Foundation of China(No.40176028)the National Key R&D Program(‘973'Program)of China(No.G1999012005).
文摘The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.
基金the Key Laboratory of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences (LFB20070601)the Key Laboratory of Mariculture of Chinese Ministry of Education,Ocean University of ChinaNational High Technology Research and Development Program of China (2007AA09Z427)
文摘The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.
