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SIRT3-FOXO1介导自噬在H9C2心肌细胞缺血再灌注损伤中的保护作用 被引量:2
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作者 王尚徐 林先和 《天津医药》 CAS 北大核心 2021年第11期1133-1137,共5页
目的探究沉默信息调节因子2相关酶类3(SIRT3)-叉头盒转录因子O1(FOXO1)通路介导自噬在心肌细胞缺血再灌注损伤中的作用。方法慢病毒感染H9C2心肌细胞构建SIRT3过表达稳定株。实验分4组:正常对照(NC)组、缺血再灌注(IR)组、SIRT3过表达+... 目的探究沉默信息调节因子2相关酶类3(SIRT3)-叉头盒转录因子O1(FOXO1)通路介导自噬在心肌细胞缺血再灌注损伤中的作用。方法慢病毒感染H9C2心肌细胞构建SIRT3过表达稳定株。实验分4组:正常对照(NC)组、缺血再灌注(IR)组、SIRT3过表达+缺血再灌注(S+IR)组、SIRT3过表达+IR+SIRT3抑制剂(S+IR+3-TYP)组。Western blot检测SIRT3、乙酰化FOXO1(Ac-FOXO1)、自噬标记蛋白LC3B、Beclin1及凋亡相关蛋白Bcl-2、Bax的表达;流式细胞分析仪检测细胞凋亡率;全自动生化仪检测细胞培养上清液中乳酸脱氢酶(LDH)水平。结果与NC组相比,IR组SIRT3表达降低,Ac-FOXO1表达升高,自噬标记蛋白LC3B、Beclin1表达升高,抗凋亡蛋白Bcl-2表达降低,促凋亡蛋白Bax表达升高,LDH水平和细胞凋亡率升高(P<0.05)。与IR组相比较,S+IR组SIRT3表达升高,Ac-FOXO1表达降低,LC3B、Beclin1表达升高,Bcl-2表达升高,Bax表达降低,LDH水平和细胞凋亡率降低(P<0.05);加入SIRT3抑制剂3-TYP后,与S+IR组相比,SIRT3表达水平无明显变化,但Ac-FOXO1表达升高,LC3B、Beclin1表达降低,Bcl-2表达降低,Bax表达升高,LDH水平和细胞凋亡率升高(P<0.05)。结论SIRT3-FOXO1通路激活可提高H9C2心肌细胞自噬水平,对心肌缺血再灌注损伤有保护作用。 展开更多
关键词 肌细胞 心脏 再灌注损伤 自噬 细胞凋亡 叉头框蛋白O1 沉默信息调节因子2相关酶类3 H9C2细胞
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Influence of inaccurate wavelet phase estimation on seismic inversion 被引量:18
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作者 Yuan San-Yi wang shang-xu 《Applied Geophysics》 SCIE CSCD 2011年第1期48-59,95,共13页
On the assumption that the seismic wavelet amplitude spectrum is estimated accurately, a group of wavelets with different phase spectra, regarded as estimated wavelets, are used to implement linear least-squares inver... On the assumption that the seismic wavelet amplitude spectrum is estimated accurately, a group of wavelets with different phase spectra, regarded as estimated wavelets, are used to implement linear least-squares inversion. During inversion, except for the wavelet phase, all other factors affecting inversion results are not taken into account. The inversion results of a sparse reflectivity model (or blocky impedance model) show that: (1) although the synthetic data using inversion results matches well with the original seismic data, the inverted reflectivity and acoustic impedance are different from that of the real model. (2) the inversion result reliability is dependent on the estimated wavelet Z transform root distribution. When the estimated wavelet Z transform roots only differ from that of the real wavelet near the unit circle, the inverted reflectivity and impedance are usually consistent with the real model; (3) although the synthetic data matches well with the original data and the Cauchy norm (or modified Cauchy norm) with a constant damping parameter has been optimized, the inverted results are still greatly different from the real model. Finally, we suggest using the L1 norm, Kurtosis, variation, Cauchy norm with adaptive damping parameter or/and modified Cauchy norm with adaptive damping parameter as evaluation criteria to reduce the bad influence of inaccurate wavelet phase estimation and obtain good results in theory. 展开更多
关键词 PHASE seismic wavelet INVERSION evaluation criterion ROOT
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Phase estimation in bispectral domain based on conformal mapping and applications in seismic wavelet estimation 被引量:8
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作者 Yu Yong-Cai wang shang-xu +1 位作者 Yuan San-Yi Qi Peng-Fei 《Applied Geophysics》 SCIE CSCD 2011年第1期36-47,95,共13页
Seismic wavelet estimation is an important part of seismic data processing and interpretation, whose preciseness is directly related to the results of deconvolution and inversion. Wavelet estimation based on higher-or... Seismic wavelet estimation is an important part of seismic data processing and interpretation, whose preciseness is directly related to the results of deconvolution and inversion. Wavelet estimation based on higher-order spectra is an important new method. However, the higher-order spectra often have phase wrapping problems, which lead to wavelet phase spectrum deviations and thereby affect mixed-phase wavelet estimation. To solve this problem, we propose a new phase spectral method based on conformal mapping in the bispectral domain. The method avoids the phase wrapping problems by narrowing the scope of the Fourier phase spectrum to eliminate the bispectral phase wrapping influence in the original phase spectral estimation. The method constitutes least-squares wavelet phase spectrum estimation based on conformal mapping which is applied to mixed-phase wavelet estimation with the least-squares wavelet amplitude spectrum estimation. Theoretical model and actual seismic data verify the validity of this method. We also extend the idea of conformal mapping in the bispectral wavelet phase spectrum estimation to trispectral wavelet phase spectrum estimation. 展开更多
关键词 conformal mapping higher-order spectra phase wrapping wavelet estimation
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Velocity dispersion and fluid substitution in sandstone under partially saturated conditions 被引量:5
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作者 Ma Xiao-Yi wang shang-xu +2 位作者 Zhao Jian-Guo Yin Han-Jun Zhao Li-Ming 《Applied Geophysics》 SCIE CSCD 2018年第2期188-196,361,362,共11页
The elastic moduli of four sandstone samples are measured at seismic (2-2000 Hz) and ultrasonic (1 MHz) frequencies and water- and glycerin-saturated conditions. We observe that the high-permeability samples under... The elastic moduli of four sandstone samples are measured at seismic (2-2000 Hz) and ultrasonic (1 MHz) frequencies and water- and glycerin-saturated conditions. We observe that the high-permeability samples under partially water-saturated conditions and the low-permeability samples under partially glycerin-saturated conditions show little dispersion at low frequencies (2-2000 Hz). However, the high-permeability samples under partially glycerin-saturated conditions and the low-permeability samples under partially water-saturated conditions produce strong dispersion in the same frequency range (2-2000 Hz). This suggests that fluid mobility largely controls the pore-fluid movement and pore pressure in a porous medium. High fluid mobility facilitates pore-pressure equilibration either between pores or between heterogeneous regions, resulting in a low-frequency domain where the Gassmann equations are valid. In contrast, low fluid mobility produces pressure gradients even at seismic frequencies, and thus dispersion. The latter shows a systematic shift to lower frequencies with decreasing mobility. Sandstone samples showed variations in Vp as a function of fluid saturation. We explore the applicability of the Gassmann model on sandstone rocks. Two theoretical bounds for the P-velocity are known, the Gassmann-Wood and Gassmann-Hill limits. The observations confirm the effect of wave-induced flow on the transition from the Gassmann-Wood to the Gassmann-Hill limit. With decreasing fluid mobility, the P-velocity at 2-2000 Hz moves from the Gassmann-Wood boundary to the Gassmann-Hill boundary. In addition,, we investigate the mechanisms responsible for this transition. 展开更多
关键词 SANDSTONE SATURATION P-WAVE DISPERSION Gassmann fluid substitution
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Incremental semi-supervised learning for intelligent seismic facies identification 被引量:1
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作者 He Su-Mei Song Zhao-Hui +2 位作者 Zhang Meng-Ke Yuan San-Yi wang shang-xu 《Applied Geophysics》 SCIE CSCD 2022年第1期41-52,144,共13页
Intelligent seismic facies identification based on deep learning can alleviate the time-consuming and labor-intensive problem of manual interpretation,which has been widely applied.Supervised learning can realize faci... Intelligent seismic facies identification based on deep learning can alleviate the time-consuming and labor-intensive problem of manual interpretation,which has been widely applied.Supervised learning can realize facies identification with high efficiency and accuracy;however,it depends on the usage of a large amount of well-labeled data.To solve this issue,we propose herein an incremental semi-supervised method for intelligent facies identification.Our method considers the continuity of the lateral variation of strata and uses cosine similarity to quantify the similarity of the seismic data feature domain.The maximum-diff erence sample in the neighborhood of the currently used training data is then found to reasonably expand the training sets.This process continuously increases the amount of training data and learns its distribution.We integrate old knowledge while absorbing new ones to realize incremental semi-supervised learning and achieve the purpose of evolving the network models.In this work,accuracy and confusion matrix are employed to jointly control the predicted results of the model from both overall and partial aspects.The obtained values are then applied to a three-dimensional(3D)real dataset and used to quantitatively evaluate the results.Using unlabeled data,our proposed method acquires more accurate and stable testing results compared to conventional supervised learning algorithms that only use well-labeled data.A considerable improvement for small-sample categories is also observed.Using less than 1%of the training data,the proposed method can achieve an average accuracy of over 95%on the 3D dataset.In contrast,the conventional supervised learning algorithm achieved only approximately 85%. 展开更多
关键词 seismic facies identification semi-supervised learning incremental learning cosine similarity
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Suppression of Mono-Frequency Interference on Seismic Record by Chirp-Z Transform Spectrum Analysis
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作者 Luo Guo-an Gao Shao-wu +1 位作者 Wei Geng-yu wang shang-xu 《石油地球物理勘探》 EI CSCD 北大核心 2009年第A02期37-44,共8页
关键词 线性调频Z变换 频谱分析技术 单频干扰 地震记录 CHIRP 扫描频率 拟合方法 地震数据处理
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岩石跨频段实验研究 被引量:4
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作者 马霄一 王尚旭 +2 位作者 赵建国 赵立明 龙腾 《地球物理学进展》 CSCD 北大核心 2018年第5期1943-1950,共8页
为了在实验室获得直接的频散测量,我们已经开发了系统的测量技术来研究岩石的弹性性质,它涵盖了从地震频率范围到超声波频带(也称为跨频带测量系统).该系统包括应力-应变测量法和传统超声法,我们利用这两种方法得到了4块岩样(2块砂岩,2... 为了在实验室获得直接的频散测量,我们已经开发了系统的测量技术来研究岩石的弹性性质,它涵盖了从地震频率范围到超声波频带(也称为跨频带测量系统).该系统包括应力-应变测量法和传统超声法,我们利用这两种方法得到了4块岩样(2块砂岩,2块碳酸盐岩)的纵、横波速度.实验结果表明:砂岩在水或者甘油饱和条件下,速度均会出现频散,水饱和时频散幅度更大;碳酸盐岩在水或者甘油饱和条件下,没有明显的频散现象.同时,计算了地震频率和超声频率下的流体因子敏感系数,发现流体识别因子中泊松阻抗最为敏感. 展开更多
关键词 频率 流体 流体因子 砂岩 碳酸盐岩
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