目的探讨过敏性鼻炎患者血清辅助性T细胞17(Th17)/调节性T细胞(Treg)值与血清甲壳质酶蛋白-40(YKL-40)水平及维生素D水平的相关性。方法选择过敏性鼻炎患者99例(观察组)及同期体检健康者99例(对照组),采集空腹外周静脉血9 m L,采用酶联...目的探讨过敏性鼻炎患者血清辅助性T细胞17(Th17)/调节性T细胞(Treg)值与血清甲壳质酶蛋白-40(YKL-40)水平及维生素D水平的相关性。方法选择过敏性鼻炎患者99例(观察组)及同期体检健康者99例(对照组),采集空腹外周静脉血9 m L,采用酶联免疫吸附试验检测外周血25(OH)D_(3),采用流式细胞术检测外周血CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比,采用双抗体夹心酶联免疫吸附法测定血清YKL-40,比较两组CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比、YKL-40及维生素D水平。结果观察组血清25(OH)D_(3)水平和CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比低于对照组(P均<0.05),CD4^(+)IL-17+Th17细胞占CD4^(+)T细胞的百分比、Th17/Treg值及血清YKL-40水平高于对照组(P均<0.05)。观察组血清Th17/Treg值与YKL-40水平呈正相关(r=0.429,P<0.05),与血清25(OH)D_(3)水平呈负相关(r=-0.735,P<0.05);血清YKL-40与25(OH)D_(3)水平呈负相关(r=-0.450,P<0.05)。结论过敏性鼻炎患者血清25(OH)D_(3)水平降低,YKL-40水平升高,二者异常表达可能与Th17/Treg值失调有关,共同参与过敏性鼻炎的发生发展。展开更多
A new continuous catalytic reforming model was configured by using a molecule-based reactor module. Themodel was based on the Sinopec Research Institute of Petroleum Processing Co., Ltd. continuous catalytic reformer ...A new continuous catalytic reforming model was configured by using a molecule-based reactor module. Themodel was based on the Sinopec Research Institute of Petroleum Processing Co., Ltd. continuous catalytic reformer fullmodel, and was reduced to a size of 157 naphtha molecules (C1−C12) that underwent 764 reactions. The new model inheritedthe advantages of the original model, and had better solving performance and flexibility owing to support by the AspenHYSYS environment. Typical commercial plant data were selected for model validation, which showed advantages in theaccuracy of detailed predictions and the range of its application. In addition, the solving time was reduced from minutes toseconds. Therefore, the simplified model proved to be feasible for industrial application.展开更多
目的分析甲状腺癌组织GATA结合蛋白3(GATA binding protein 3,GATA3)蛋白表达情况及其与临床病理特征和手术预后的关系。方法选取内蒙古锡林郭勒盟中心医院2016年8月~2017年12月收治的150例采用手术治疗的甲状腺癌患者,取癌组织与切缘...目的分析甲状腺癌组织GATA结合蛋白3(GATA binding protein 3,GATA3)蛋白表达情况及其与临床病理特征和手术预后的关系。方法选取内蒙古锡林郭勒盟中心医院2016年8月~2017年12月收治的150例采用手术治疗的甲状腺癌患者,取癌组织与切缘正常组织采用免疫组化法检测GATA3蛋白表达,比较癌组织和切缘正常组织的GATA3蛋白表达量。比较不同临床病理特征患者甲状腺癌组织GATA3蛋白表达量;随访5年分析癌组织GATA3蛋白表达与手术预后的关系。结果甲状腺癌组织GATA3蛋白表达量低于切缘正常组织(P<0.05);未分化癌和髓样癌、TNM分期Ⅲ~Ⅳ期、多发病灶、最大肿瘤直径≥2 cm、有淋巴结转移患者癌组织GATA3蛋白表达量分别低于乳头状腺癌和滤泡状腺癌、TNM分期Ⅰ~Ⅱ期、单发病灶、最大肿瘤直径<2 cm、无淋巴结转移患者(P<0.05);随访期间甲状腺癌患者生存率为83.45%、死亡率为16.55%;未分化癌(RR=1.772,95%CI:1.221~2.571)、髓样癌(RR=2.423,95%CI:1.609~3.650)、TNM分期Ⅲ~Ⅳ期(RR=2.020,95%CI:1.447~2.818)、多发病灶(RR=1.914,95%CI:1.421~2.578)、最大肿瘤直径≥2 cm(RR=1.818,95%CI:1.129~2.928)、有淋巴结转移(RR=1.937,95%CI:1.241~3.022)、GATA3蛋白表达量(RR=0.488,95%CI:0.333~0.715)均为甲状腺癌患者死亡的影响因素(P<0.05);甲状腺癌组织GATA3蛋白高表达患者生存率高于GATA3蛋白低表达患者(P<0.05)。结论GATA3蛋白在甲状腺癌组织中呈低表达,且GATA3蛋白表达与病理类型、TNM分期、病灶数目、肿瘤大小及淋巴结转移情况有关,上述指标均是患者手术预后的影响因素。展开更多
文摘目的探讨过敏性鼻炎患者血清辅助性T细胞17(Th17)/调节性T细胞(Treg)值与血清甲壳质酶蛋白-40(YKL-40)水平及维生素D水平的相关性。方法选择过敏性鼻炎患者99例(观察组)及同期体检健康者99例(对照组),采集空腹外周静脉血9 m L,采用酶联免疫吸附试验检测外周血25(OH)D_(3),采用流式细胞术检测外周血CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比,采用双抗体夹心酶联免疫吸附法测定血清YKL-40,比较两组CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比、YKL-40及维生素D水平。结果观察组血清25(OH)D_(3)水平和CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比低于对照组(P均<0.05),CD4^(+)IL-17+Th17细胞占CD4^(+)T细胞的百分比、Th17/Treg值及血清YKL-40水平高于对照组(P均<0.05)。观察组血清Th17/Treg值与YKL-40水平呈正相关(r=0.429,P<0.05),与血清25(OH)D_(3)水平呈负相关(r=-0.735,P<0.05);血清YKL-40与25(OH)D_(3)水平呈负相关(r=-0.450,P<0.05)。结论过敏性鼻炎患者血清25(OH)D_(3)水平降低,YKL-40水平升高,二者异常表达可能与Th17/Treg值失调有关,共同参与过敏性鼻炎的发生发展。
基金The authors acknowledge collaboration with and support from AspenTech via the National Key R&D Program of China(2021YFA1501201).
文摘A new continuous catalytic reforming model was configured by using a molecule-based reactor module. Themodel was based on the Sinopec Research Institute of Petroleum Processing Co., Ltd. continuous catalytic reformer fullmodel, and was reduced to a size of 157 naphtha molecules (C1−C12) that underwent 764 reactions. The new model inheritedthe advantages of the original model, and had better solving performance and flexibility owing to support by the AspenHYSYS environment. Typical commercial plant data were selected for model validation, which showed advantages in theaccuracy of detailed predictions and the range of its application. In addition, the solving time was reduced from minutes toseconds. Therefore, the simplified model proved to be feasible for industrial application.
