玉米紫色植株色素预干预对过氧化氢致成骨细胞氧化损伤的影响

目的:研究玉米紫色植株色素(maize purple plant pigment,MPPP)预干预对过氧化氢(H2O2)诱导的MC3T3-E1成骨细胞氧化损伤的影响。方法:采用MTT法观察浓度为10-10～10-3mol/L的H2O2对成骨细胞增殖能力的影响,并选择最佳浓度建立MC3T3-E1成骨细胞氧化损伤模型(H2O2处理组);同时选择MPPP促进成骨细胞增殖的适宜浓度,采用化学比色法观察H2O2分别对低(10-7mol/L)、中(10-6mol/L)、高(10-5mol/L)浓度的MPPP预干预24、48和72 h后的成骨细胞活力及MPPP预干预72 h后各组细胞总抗氧化力(T-AOC)的影响。结果:10-7～10-3mol/L的H2O2均可抑制成骨细胞增殖(P<0.01)。不同浓度MPPP在不同预干预时间条件下,H2O2(5×10-4mol/L)处理后各组细胞OD值均显著低于对照组(P<0.05或0.01),仅预干预72 h的高浓度MPPP组细胞OD值高于H2O2处理组(P<0.05);MPPP预干预72 h后,与对照组比较,H2O2处理组和低、中、高浓度MPPP组成骨细胞T-AOC均显著降低(P<0.01),但高浓度MPPP组成骨细胞T-AOC水平明显高于H2O2处理组(P<0.01)。结论:MPPP预干预可抑制H2O2诱导的MC3T3-E1成骨细胞的氧化损伤并提升成骨细胞T-AOC。

Commercial Test of Two Diesel Demulsifiers

PetroChina Lanzhou Petrochemical Company conducted commercial tests for application of two types of diesel demulsifiers, the HPL-2 and GX-02 demulsifiers, in order to solve the emulsion problem arising from caustic washing of straight-run diesel fraction obtained from atmospheric and vacuum distillation unit at the Lanzhou refinery. After addition of each demulsifier into the diesel fraction the oil content in caustic residue was apparently decreased, and discharge of waste caustic was reduced, resulting in the elimination of emulsification and a significant increase of economic benefits. When 70 ppm of the HPL-2 demulsifier was added to diesel fraction, the oil content in waste caustic exiting the second-stage caustic wash settling tank was reduced to 2.45% from 7.90 %, whereas this value was reduced to 2.81% from 5.96% with addition of the GX-02 demulsifier.