Tumour response following preoperative chemotherapy is affected by body mass index in patients with colorectal liver metastases

BACKGROUND Colorectal cancer is the third most prevalent malignancy globally and ranks second in cancer-related mortality,with the liver being the primary organ of metastasis.Preoperative chemotherapy is widely recommended for initially or potentially resectable colorectal liver metastases(CRLMs).Tumour pathological response serves as the most important and intuitive indicator for assessing the efficacy of chemotherapy.However,the postoperative pathological results reveal that a considerable number of patients exhibit a poor response to preoperative chemotherapy.Body mass index(BMI)is one of the factors affecting the tumori-genesis and progression of colorectal cancer as well as prognosis after various antitumour therapies.Several studies have indicated that overweight and obese patients with metastatic colorectal cancer experience worse prognoses than those with normal weight,particularly when receiving first-line chemotherapy regimens in combination with bevacizumab.AIM To explore the predictive value of BMI regarding the pathologic response following preoperative chemotherapy for CRLMs.METHODS A retrospective analysis was performed in 126 consecutive patients with CRLM who underwent hepatectomy following preoperative chemotherapy at four different hospitals from October 2019 to July 2023.Univariate and multivariate logistic regression models were applied to analyse potential predictors of tumour pathological response.The Kaplan-Meier method with log rank test was used to compare progression-free survival(PFS)between patients with high and low BMI.BMI<24.0 kg/m^(2) was defined as low BMI,and tumour regression grade 1-2 was defined as complete tumour response.RESULTS Low BMI was observed in 74(58.7%)patients and complete tumour response was found in 27(21.4%)patients.The rate of complete tumour response was significantly higher in patients with low BMI(29.7%vs 9.6%,P=0.007).Multivariate analysis revealed that low BMI[odds ratio(OR)=4.56,95%confidence interval(CI):1.42-14.63,P=0.011],targeted therapy with bevacizumab(OR=3.02,95%CI:1.10-8.33,P=0.033),preoperative carcinoembryonic antigen level<10 ng/mL(OR=3.84,95%CI:1.19-12.44,P=0.025)and severe sinusoidal dilatation(OR=0.17,95%CI:0.03-0.90,P=0.037)were independent predictive factors for complete tumour response.The low BMI group exhibited a significantly longer median PFS than the high BMI group(10.7 mo vs 4.7 mo,P=0.011).CONCLUSION In CRLM patients receiving preoperative chemotherapy,a low BMI may be associated with better tumour response and longer PFS.

妊娠糖尿病患者合并妊娠高血压的风险预测模型构建

目的探讨妊娠糖尿病(GDM)患者合并妊娠高血压的危险因素,并建立列线图预测模型。方法将294例GDM患者按照2∶1比例分为建模组(196例)和验证组(98例)。根据是否合并妊娠高血压将建模组分为合并高血压组(51例)和未合并高血压组(145例)。分析GDM合并妊娠高血压的影响因素,构建列线图模型并验证。结果孕前超重/肥胖、孕期体质量增长过度、口服葡萄糖耐量试验3项异常、同型半胱氨酸(Hcy)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、胰岛素抵抗(HOMA-IR)、丙二醛(MDA)、合并焦虑/抑郁情绪均是GDM合并妊娠高血压的影响因素(OR=2.776、2.149、5.008、4.586、4.208、4.047、3.473、0.503、3.688、3.317、2.305,P<0.05)。将上述影响因素作为预测指标,构建GDM合并妊娠高血压风险预测列线图模型,建模组和验证组一致性指数分别为0.830、0.827,校准曲线和理想曲线拟合度均较好。受试者工作特征曲线评估模型显示,建模组、验证组曲线下面积分别为0.859、0.850;决策曲线显示,建模组、验证组在风险阈值概率分别为0.02~0.89、0.02~0.85时可获得较高的净获益值。结论孕前超重/肥胖、孕期体质量增长过度、口服葡萄糖耐量试验3项异常、Hcy、TG、TC、LDL、HDL、HOMA-IR、MDA、合并焦虑/抑郁情绪均是GDM合并妊娠高血压的影响因素,据此构建的列线图模型预测效能较好,可指导临床筛选高风险人群。

Design and assembly of a nested imaging X-ray telescope for the Hot Universe Baryon Surveyor mission

The Hot Universe Baryon Surveyor (HUBS) mission will carry a nested X-ray telescope capable of observing an energy range from 0.5 keV to 2 keV to study hot baryon evolution. In this paper, we report the latest progress in the design and construction of nested X-ray telescopes which were designed to use a three-stage conic-approximation type assembly to simplify the manufacturing process. The mirror substrate is made using the thermal glass slumping method, with mirrors characterized by a root-mean-square roughness of 0.3 nm, with expected high reflectivity and good thermal stability. We also discuss methods of telescope construction and conduct a deformation analysis of the manufactured mirror. The in situ measurement system program is developed to guide the telescope assembly process.

清远一次极端龙舟水的中尺度系统演变分析

利用多源观测数据和ERA5再分析资料,对2022年6月20到21日发生在清远的一次极端特大暴雨过程进行分析。结果表明:(1)该次强降水是在200 hPa分流辐散的背景下,由低涡切变配合其前侧西南急流缓慢东移经过清远造成的。(2)强降水发生前大气层结已处于极不稳定状态,低层来自孟加拉湾和南海的水汽不断在清远市上空辐合,为暴雨的发生提供充足的水汽条件和不稳定能量。(3)强降水的触发机制主要是前期广西降水造成的冷池与低空急流带来的暖湿气团形成的温度锋区和地面辐合线;地形的抬升作用和狭管效应,对降水起到增幅作用。(4)该次强降水主要有2个低质心的热带海洋型中尺度对流系统先后影响发生。

ITGA3表达与结直肠癌免疫细胞浸润的关系

目的:探讨结直肠癌(colorectal cancer,CRC)中整合素亚蛋白ɑ3(integrin subunitɑ3,ITGA3)的表达与免疫细胞浸润之间的关系。方法:利用生物信息学方法分析ITGA3 mRNA在泛癌及CRC组织中的表达及对预后的影响,同时全面研究ITGA3与肿瘤浸润免疫细胞的相关性。收集2021年1月至2021年12月山西省肿瘤医院确诊为CRC的病例233例,进一步采用免疫组织化学染色(IHC)检测ITGA3、CD8、CD163、FOXP3、PD-L1、CTLA-4、PD-1在CRC组织中的表达,分析ITGA3与免疫浸润细胞、免疫检查点之间的关系。结果:生信分析结果显示ITGA3 mRNA在CRC中表达升高;ITGA3高表达与无进展生存期(progression-free survival,PFS)相关(P<0.05);单因素、多因素分析显示年龄、分期与患者的预后显著相关(P<0.05)。此外,上调的ITGA3表达与CRC中多种免疫细胞浸润水平密切相关。IHC结果表明ITGA3在CRC组织中的高表达率明显高于癌旁正常组织(P<0.05)。ITGA3的表达与淋巴结转移相关(P<0.05),与CD8^(+)T细胞、PD-L1、CTLA-4(均P<0.05)等免疫标志物表达相关。结论:ITGA3在CRC中高表达,与免疫细胞浸润等密切相关,其可能调节肿瘤免疫微环境,为临床治疗及预后判断提供了新的思路。

大数据时代高校新工科人才培养教学改革探析

大数据时代对工科人才的知识结构和能力素质提出了新要求。面对大数据带来的思维方式和教学模式的改变,高校通过学科交叉和实践教学来提升新工科人才的创新能力和工程实践能力,但仍然存在不足。本文梳理了大数据产业对新工科人才的能力要求,深入剖析了大数据时代新工科人才培养中存在的问题,并提出了针对性的改革举措,旨在为相关人员提供经验,帮助提升新工科人才培养质量。

区域整体推进数字教育公共服务实践与思考——以山东省聊城市为例

“十四五”时期,教育信息化进入新发展阶段,要求区域数字教育公共服务体系建设要贯彻新发展理念、构建新发展格局、营造新发展环境。该研究从背景概述、数字教育公共服务体系构建、工作进展与成效、工作问题与反思、未来规划与展望等五个维度,系统阐释了聊城市在区域数字教育公共服务体系建设中的探索与实践:基于区域实际,以“应用为王、服务至上、示范引领、安全运行”为指导原则,强化顶层设计与规划,明确工作目标与定位,创新工作机制与方法,探索区域视野下整体推进数字教育公共服务体系建设的创新实践之路;借助信息技术赋能,提升教育教学环境、优化教育资源供给、加快教学模式变革,构建绿色、开放、互联、共享的教育生态体系,加快推进区域教育高质量均衡发展。

Bioorthogonal Engineered Virus‑Like Nanoparticles for Efficient Gene Therapy

Gene therapy offers potentially transformative strategies for major human diseases.However,one of the key challenges in gene therapy is developing an effective strategy that could deliver genes into the specific tissue.Here,we report a novel virus-like nanoparticle,the bioorthgonal engineered viruslike recombinant biosome(reBiosome),for efficient gene therapies of cancer and inflammatory diseases.The mutant virus-like biosome(mBiosome)is first prepared by site-specific codon mutation for displaying 4-azido-L-phenylalanine on vesicular stomatitis virus glycoprotein of eBiosome at a rational site,and the reBiosome is then prepared by clicking weak acid-responsive hydrophilic polymer onto the mBiosome via bioorthogonal chemistry.The results show that the reBiosome exhibits reduced virus-like immunogenicity,prolonged blood circulation time and enhanced gene delivery efficiency to weakly acidic foci(like tumor and arthritic tissue).Furthermore,reBiosome demonstrates robust therapeutic efficacy in breast cancer and arthritis by delivering gene editing and silencing systems,respectively.In conclusion,this study develops a universal,safe and efficient platform for gene therapies for cancer and inflammatory diseases.

Preliminary results of the global ocean tide derived from HY-2A radar altimeter data

The HY-2A satellite,which is equipped with a radar altimeter and was launched on August 16,2011,is the first Chinese marine dynamic environmental monitoring satellite.Extracting ocean tides is one of the important applications of the radar altimeter data.The radar altimeter data of the HY-2A satellite from November 1,2011 to August 16,2014 are used herein to extract global ocean tides.The constants representing the tidal constituents are extracted by HY-2A RA data with harmonic analysis based on the least squares method.Considering tide aliasing issues,the analysis of the alias periods and alias synodic periods of different tidal constituents shows that only the tidal constituents M_(2),N_(2),and K_(2)are retrieved precisely by the HY-2A RA data.The derived tidal constants of the tidal constituents M_(2),N_(2)and K_(2)are compared to those of tidal gauge data and the TPXO tide model results.The comparison between the derived results and the tidal gauge data shows that the RMSEs of the tidal amplitude and phase lag are 9.6 cm and 13.34°,2.4 cm and 10.47°,and 8.1 cm and 14.19°for tidal constituents M_(2),N_(2),and K_(2),respectively.The comparisons of the semidiurnal tides with the TPXO model results show that tidal constituents have good consistency with the TPXO model results.These findings confirm the good performance of HY-2A RA for retrieving semidiurnal tides in the global ocean.

Dimensionality reduction model based on integer planning for the analysis of key indicators affecting life expectancy

Purpose:Exploring a dimensionality reduction model that can adeptly eliminate outliers and select the appropriate number of clusters is of profound theoretical and practical importance.Additionally,the interpretability of these models presents a persistent challenge.Design/methodology/approach:This paper proposes two innovative dimensionality reduction models based on integer programming(DRMBIP).These models assess compactness through the correlation of each indicator with its class center,while separation is evaluated by the correlation between different class centers.In contrast to DRMBIP-p,the DRMBIP-v considers the threshold parameter as a variable aiming to optimally balances both compactness and separation.Findings:This study,getting data from the Global Health Observatory(GHO),investigates 141 indicators that influence life expectancy.The findings reveal that DRMBIP-p effectively reduces the dimensionality of data,ensuring compactness.It also maintains compatibility with other models.Additionally,DRMBIP-v finds the optimal result,showing exceptional separation.Visualization of the results reveals that all classes have a high compactness.Research limitations:The DRMBIP-p requires the input of the correlation threshold parameter,which plays a pivotal role in the effectiveness of the final dimensionality reduction results.In the DRMBIP-v,modifying the threshold parameter to variable potentially emphasizes either separation or compactness.This necessitates an artificial adjustment to the overflow component within the objective function.Practical implications:The DRMBIP presented in this paper is adept at uncovering the primary geometric structures within high-dimensional indicators.Validated by life expectancy data,this paper demonstrates potential to assist data miners with the reduction of data dimensions.Originality/value:To our knowledge,this is the first time that integer programming has been used to build a dimensionality reduction model with indicator filtering.It not only has applications in life expectancy,but also has obvious advantages in data mining work that requires precise class centers.

Integrin beta 3-overexpressing mesenchymal stromal cells display enhanced homing and can reduce atherosclerotic plaque

BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular disease.Integrin beta 3(ITGB3)promotes cell migration in several cell types.However,whether ITGBmodified MSCs can migrate to plaque sites in vivo and play an anti-atherosclerotic role remains unclear.AIM To investigate whether ITGB3-overexpressing MSCs(MSCs^(ITGB3))would exhibit improved homing efficacy in atherosclerosis.METHODS UC MSCs were isolated and expanded.Lentiviral vectors encoding ITGB3 or green fluorescent protein(GFP)as control were transfected into MSCs.Sixty male apolipoprotein E-/-mice were acquired from Beijing Vital River Lab Animal Technology Co.,Ltd and fed with a high-fat diet(HFD)for 12 wk to induce the formation of atherosclerotic lesions.These HFD-fed mice were randomly separated into three clusters.GFP-labeled MSCs(MSCs^(GFP))or MSCs^(ITGB3)were transplanted into the mice intravenously via the tail vein.Immunofluorescence staining,Oil red O staining,histological analyses,western blotting,enzymelinked immunosorbent assay,and quantitative real-time polymerase chain reaction were used for the analyses.RESULTS ITGB3 modified MSCs successfully differentiated into the“osteocyte”and“adipocyte”phenotypes and were characterized by positive expression(>91.3%)of CD29,CD73,and CD105 and negative expression(<1.35%)of CD34 and Human Leukocyte Antigen-DR.In a transwell assay,MSCs^(ITGB3)showed significantly faster migration than MSCsGFP.ITGB3 overexpression had no effects on MSC viability,differentiation,and secretion.Immunofluorescence staining revealed that ITGB3 overexpression substantially enhanced the homing of MSCs to plaque sites.Oil red O staining and histological analyses further confirmed the therapeutic effects of MSCs^(ITGB3),significantly reducing the plaque area.Enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction revealed that MSC^(ITGB3)transplantation considerably decreased the inflammatory response in pathological tissues by improving the dynamic equilibrium of pro-and anti-inflammatory cytokines.CONCLUSION These results showed that ITGB3 overexpression enhanced the MSC homing ability,providing a potential approach for MSC delivery to plaque sites,thereby optimizing their therapeutic effects.

Experimental Study on the Treatment of Tertiary Oil Recovery Wastewater via a Novel Electro-Coagulation Method

At present,methods for treating tertiary oil recovery wastewater via electro-coagulation are still in their early stage of development.In this study,a device for electro-coagulation wastewater treatment was built and tested in an oil field.The effects that the initial pH value,electrode type,and connection mode have on the coagulation and separation effect were assessed by measuring the mass fraction and turbidity of oil.The results have shown that when the electro-coagulation method is used,the effectiveness of the treatment can be significantly increased in neutral pH conditions(pH=7),in acidic conditions,and in alkaline conditions.Compared to an Al electrode,the floc that is produced by an Fe electrode is smaller;thus,it does not easily coagulate and settle in a short time.Using the oil removal rate,turbidity removal rate and energy consumption as a basis to assess the performances,the results have demonstrated that the combined aluminum alloy iron composite electrode should be used as electrolytic electrode.

人胎盘来源的间充质干细胞移植对递增负荷训练诱导的POI大鼠卵巢功能的影响

随着女性生育年龄的推迟,30~39岁早发性卵巢功能不全(premature ovarian insufficiency,POI)患者的治疗需求增加。先前的研究证实,不同组织来源的干细胞可以恢复POI小鼠的卵巢功能。本研究旨在探讨人胎盘间充质干细胞(human placenta-derived mesenchymal stem cell,hpMSC)移植治疗递增负荷训练诱导的POI的效果和潜在机制。首先,采用为期9周的递增负荷跑台训练建立POI大鼠模型;然后,通过尾静脉注射给予hpMSC移植治疗;最后,通过激素水平和组织学分析评估卵巢功能,利用hpMSC示踪、酶联免疫吸附试验(en-zyme linked immunosorbent assay,ELISA)、免疫印迹分析、免疫组织化学染色和实时荧光定量PCR评估损伤和修复的分子机制。结果显示,移植后的hpMSC定植在卵巢间质;hpMSC移植增加了卵母细胞的端粒长度,减少了氧化应激反应,下调了Bax(Bcl-2-associated X protein)和胱天蛋白酶-3(caspase-3)的蛋白质水平,与此同时,卵泡刺激素(follicle-stimulating hormone,FSH)的水平、FSH与黄体生成素(luteinizing hormone,LH)的比值(FSH/LH)下降,各级卵泡数量增加。实验结果表明,hpMSC移植可以修复递增负荷训练诱导的POI大鼠的卵巢损伤,改善卵巢功能。

集束化护理干预在鼻咽癌患者放射性皮炎防治中的应用

目的探讨集束化护理干预在鼻咽癌患者放射性皮炎防治中的应用。方法选取2019年1月至2021年2月广西壮族自治区梧州市红十字会医院肿瘤科接受首次根治性放疗的鼻咽癌患者210例为研究对象,采用随机数字表分为观察组和对照组,研究过程中依从性差退出2例、病情变化转科2例,最后纳入206例鼻咽癌患者为研究对象,其中观察组105例,对照组101例。对照组实施常规护理,观察组在对照组的基础上实施基于循证的集束化护理干预。观察评估患者放疗后各级皮炎的发生率和发生时间、皮炎的严重程度、皮肤损伤体征出现的时间。结果两组皮炎发生率比较,差异无统计学意义(P>0.05)。观察组皮肤损伤体征出现的时间长于对照组、皮炎总得分低于对照组(P<0.05)。两组Log-rank检验分析显示观察组无2级皮炎的时间长于对照组(P<0.05)。结论与常规护理比较,集束化护理干预可以延缓放射性皮炎的发生,降低皮炎的严重程度。

不同手术时机对胸腰段椎体骨折疗效的影响

目的探讨不同时机手术对胸腰段椎体骨折伴不完全性脊髓损伤患者临床疗效及复位效果的影响。方法回顾性分析2010年1月-2015年1月手术治疗的胸腰段椎体骨折伴脊髓不完全损伤患者的资料,按手术距离受伤的时间将其分为两组:伤后72 h内手术者为A组;≥72 h者为B组。A组40例,年龄21~62岁,T11 3例,T12 10例,L1 15例,L2 12例。B组39例,年龄20~69岁,T11 2例,T12 9例,L1 16例,L2 12例。比较两组术后脊髓功能恢复情况及矫形效果。结果随访24~47个月,平均(31.3±9.8)个月。两组均无术后感染、继发性脊髓神经损伤、内固定松动的发生。A组40例中15例进行椎管内减压(37.5%),B组39例中33例进行椎管内减压(84.6%)(P<0.05)。术前两组美国脊髓损伤协会(ASIA)分级比较,差异无统计学意义(P>0.05);术后两组间ASIA分级比较,差异有统计学意义(P <0.05)。术后两组感觉评分和运动评分比较,A组感觉评分和运动评分均高于B组(P <0.05);术前两组伤椎前缘高度压缩比及伤椎Cobb角比较,差异无统计学意义(P>0.05),术后两组比较,差异有统计学意义(P <0.05),A组椎体高度的恢复、矫形效果高于B组。结论对胸腰段椎体爆裂骨折伴不完全性脊髓损伤的患者,伤后72 h内进行手术治疗可取得较好的临床疗效和矫形效果,72 h内手术利于进入椎管内的骨折块复位可使部分患者避免椎管内减压。

简便艾灸盒在婴幼儿腹泻治疗中的应用

目的探讨简便艾灸盒在治疗婴幼儿腹泻中的应用效果及护理要点。方法 168例腹泻患儿分为观察组85例和对照组83例。对照组予常规西医治疗及护理,观察组在对照组基础上应用简便艾灸盒艾灸相关穴位。观察2组腹泻疗效、呕吐消失时间及住院时间。结果观察组总有效率高于对照组(P<0.05),观察组患儿呕吐消失时间及住院时间均短于对照组(P<0.05)。结论利用简便艾灸盒艾灸治疗结合综合护理干预能有效确保婴幼儿腹泻治疗效果,促进康复。

Research Progress of Chimeric RNA and Health

With the development of deep sequencing and bioinformatics technology, a large number of products produced by abnormal RNA splicing, such as chimeric RNA and chimeric/fusion proteins, have been discovered. Natural chimeric/fusion genes are new genes formed by natural fusion of two or more independent genes. Chimeric RNAs can be transcribed by natural chimeric genes, and can also be formed by cis-splicing or trans-splicing of two or more precursor mRNAs. Unlike fusion genes, the production of chimeric RNAs does not involve changes in the DNA level of chromosomes. At first, chimeric RNAs were found as tumor markers. With the deepening of research, researchers also found a large number of chimeric RNAs in normal tissues. From the perspective of biological function, chimeric RNAs can play a biological role in regulating the expression of corresponding maternal genes, translating into chimeric proteins, and forming long non-coding RNAs. The objective of the present study focused on the frontiers of chimeric RNA and reviewed its role in health and tumor study to reveal research progress of chimeric RNA and health and provide a new sight of relative disease treatment. The main conclusion of this review is that chimeric RNA may serve as a biomarker for specific tumor diagnose and treatment while its role in normal physiology needs to be revealed.

运动血清对海马HT-22细胞突触形成及BDNF、TrkB蛋白表达的影响

目的:探讨运动血清对海马HT-22细胞突触形成和BDNF、TrkB蛋白表达量的影响。方法:测定运动员和无运动习惯人血清睾酮、皮质醇、肌酸激酶和尿素含量,分别采用运动员血清、无运动习惯人血清干预离体培养的海马HT-22细胞,观察不同血清培养下海马HT-22细胞的形态,并测定海马HT-22细胞BDNF和TrkB两种蛋白的表达量。结果:在进行运动训练后,血清中生化指标有了一定的变化;运动血清培养的HT-22细胞间的连接数量多于无运动习惯人;男性运动血清组细胞BDNF和TrkB含量均显著大于无运动习惯男性血清组(P<0.05);女性运动血清组细胞TrkB含量显著大于无运动习惯型女性血清组(P<0.05)。结论:运动血清能够增加HT-22细胞间的连接数量以及BDNF、TrkB的表达,对海马产生有益的影响。

京津冀地区科技金融生态比较研究

通过构建京津冀地区科技金融生态环境评价的指标体系,采用变异系数法对2010~2017年这一京津冀地区13个地级市2010~2017年的数据进行了动态综合评价,结果表明,京津冀该地区科技金融生态水平以京、津两市为核心呈现出“内极化”现象,具有不平衡性,主要原因在于京津两地科技金融生态发展的基础条件显著优于河北省的11个城市,同时京津对区域创新发展的带动和扩散作用不足,加以行政区划的刚性约束,三地未能形成发展合力。于是提出了以下建议:通过发挥北京市产业结构转型升级对津冀的带动作用、,落实京津冀三地的战略定位以及,持续推进京津冀地区科技金融协同的方式,以促进京津冀地区金融发展与科技领城的可持续创新。

代谢相关药物对3T3-L1脂肪细胞促酰化蛋白分泌的影响

目的:建立使用3T3-L1脂肪细胞评价药物影响促酰化蛋白(ASP)分泌的细胞模型和测定ASP分泌的ELISA方法,观察代谢相关药物二甲双胍、罗格列酮钠及立莫那班对ASP分泌的影响及机制,以及与补体C3、非酯化脂肪酸(NE-FA)释放、甘油三酯总量(TG mass)、脂肪酸摄取量的关系。方法:以3T3-L1前脂肪细胞为实验对象,诱导细胞分化,分别加入乳糜微粒、二甲双胍、罗格列酮钠及立莫那班作用48 h。采用ELISA法测定ASP、C3含量,使用酶标比色法测定NEFA含量,脂肪细胞FA摄取速度在荧光酶标仪进行动力学方法测量,采用分光光度法测定TG含量,考马斯亮蓝染料结合法测定细胞总蛋白。结果:乳糜微粒刺激ASP分泌(达411%±133%P<0.05);罗格列酮钠和立莫那班抑制ASP产生(-53%～-85%,P<0.05),同时抑制前体蛋白C3分泌(-37%～-65%,P<0.05);二甲双胍抑制ASP分泌(-54%～-100%,P<0.05),对前体蛋白C3分泌无影响;二甲双胍降低TG含量(达-60%,P<0.05)和FA摄取速度(达-75%,P<0.05)。结论:成功建立了评价药物影响促酰化蛋白(ASP)分泌的3T3-L1前脂肪细胞模型,并建立了测定ASP分泌的ELISA方法。