This study introduces a pre-orthogonal adaptive Fourier decomposition(POAFD)to obtain approximations and numerical solutions to the fractional Laplacian initial value problem and the extension problem of Caffarelli an...This study introduces a pre-orthogonal adaptive Fourier decomposition(POAFD)to obtain approximations and numerical solutions to the fractional Laplacian initial value problem and the extension problem of Caffarelli and Silvestre(generalized Poisson equation).As a first step,the method expands the initial data function into a sparse series of the fundamental solutions with fast convergence,and,as a second step,makes use of the semigroup or the reproducing kernel property of each of the expanding entries.Experiments show the effectiveness and efficiency of the proposed series solutions.展开更多
The 2009 M W 7.8 Fiordland （New Zealand） earthquake is the largest to have occurred in New Zealand since the 1931 M W 7.8 Hawke’s Bay earthquake, 1 000 km to the northwest. In this paper two tracks of ALOS PALSAR i...The 2009 M W 7.8 Fiordland （New Zealand） earthquake is the largest to have occurred in New Zealand since the 1931 M W 7.8 Hawke’s Bay earthquake, 1 000 km to the northwest. In this paper two tracks of ALOS PALSAR interferograms （one ascending and one descending） are used to determine fault geometry and slip distribution of this large earthquake. Modeling the event as dislocation in an elastic half-space suggests that the earthquake resulted from slip on a SSW-NNE orientated thrust fault that is associated with the subduction between the Pacific and Australian Plates, with oblique displacement of up to 6.3 m. This finding is consistent with the preliminary studies undertaken by the USGS using seismic data.展开更多
Hadoop framework emerged at the right moment when traditional tools were powerless in terms of handling big data. Hadoop Distributed File System (HDFS) which serves as a highly fault-tolerance distributed file system ...Hadoop framework emerged at the right moment when traditional tools were powerless in terms of handling big data. Hadoop Distributed File System (HDFS) which serves as a highly fault-tolerance distributed file system in Hadoop, can improve the throughput of data access effectively. It is very suitable for the application of handling large amounts of datasets. However, Hadoop has the disadvantage that the memory usage rate in NameNode is so high when processing large amounts of small files that it has become the limit of the whole system. In this paper, we propose an approach to optimize the performance of HDFS with small files. The basic idea is to merge small files into a large one whose size is suitable for a block. Furthermore, indexes are built to meet the requirements for fast access to all files in HDFS. Preliminary experiment results show that our approach achieves better performance.展开更多
Rapid detection of target foodborne pathogens plays more and more significant roles in food safety,which requires the efficiency,sensitivity,and accuracy.In this research,we proposed a new st rategy of isothermal-mole...Rapid detection of target foodborne pathogens plays more and more significant roles in food safety,which requires the efficiency,sensitivity,and accuracy.In this research,we proposed a new st rategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture.Th e designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min,resolving the drawbacks of traditional random sampling protocols.After simple and convenient ultrasonication,samples can be directly amplified at 39℃ in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min.Finally,satisfied results have been achieved within 40 min,which greatly improve the efficiency while the accuracy is also guaranteed.Furthermore,all detection steps can be completed under instrument-free conditions.This method will hold great promise for target pathogen detection in the resource-limited district,or for emergency on-site identification.展开更多
Doxycycline hydrochloride and florfenicol combination(DoxHcl&FF)is an effective treatment for respiratory diseases.In the study,our objective Was to evaluate the activity of DoxHcl&FF against Actinobacillus pl...Doxycycline hydrochloride and florfenicol combination(DoxHcl&FF)is an effective treatment for respiratory diseases.In the study,our objective Was to evaluate the activity of DoxHcl&FF against Actinobacillus pleuropneumoniae(APP)in porcine pulmonary epithelial lining fluid(PELF)and the optimal dosage scheme to avoid the development of resistance.The DoxHcl&FF Was administered intramuscularly(IM)at 20mg/kg,and the PELF was collected at differ-ent time points.The minimum inhibitory concentration(MIC)and time-mortality curves were also included in the study.Based on the sigmoid Emax equation and dose equations,the study integrated the in vivo pharmacokinetic data of infected pigs and ex vivo pharmacodynamic data to obtain the area under concentration time curve(AUCo-24h)MIC values in PELF and achieve bacteriostatic activity,bactericidal activity and the virtual eradication of bacteria.The study showed that the combination of DoxHcl and FF caused no significant changes in PK parameters.The peak concentration(Cmax)of FF in healthy and diseased pigs was 8.87±0.08 and 8.67±0.07μg/mL,the_AUCo-24h were.172.75±2.52 and 18022±3.13 h-μg/mL,the Cmax of DoxHcl was 7.91±0.09 and 7.99±0.05μg/mL,and the AUCo-24h was 129.96±3.70 h-μg/mL and 169.82±4.38 h-μg/mL.DoxHcl&FF showed strong concentra-tion-dependent tendencies.The bacteriostatic,bactericidal,and elimination activity were calculated as 5.61,18.83 and 32.68 h,and the doses were 1.37(bacteriostatic),4.59(bactericidal)and 7.99(elimination)mg/kg.These findings indicated that the calculated recommended dose could assist in achieving more precise administration,increasing the effectiveness of DoxHcl&FF treatment for APP infections.展开更多
BACKGROUND Primary hyperoxaluria type 1(PH1)is a rare autosomal recessive disease stemming from a deficiency in liver-specific alanine-glyoxylate aminotransferase,resulting in increased endogenous oxalate deposition a...BACKGROUND Primary hyperoxaluria type 1(PH1)is a rare autosomal recessive disease stemming from a deficiency in liver-specific alanine-glyoxylate aminotransferase,resulting in increased endogenous oxalate deposition and end-stage renal disease.Organ transplantation is the only effective treatment.However,its approach and timing remain controversial.CASE SUMMARY We retrospectively analyzed 5 patients diagnosed with PH1 from the Liver Transplant Center of the Beijing Friendship Hospital from March 2017 to December 2020.Our cohort included 4 males and 1 female.The median age at onset was 4.0 years(range:1.0-5.0),age at diagnosis was 12.2 years(range:6.7-23.5),age at liver transplantation(LT)was 12.2 years(range:7.0-25.1),and the follow-up time was 26.3 mo(range:12.8-40.1).All patients had delayed diagnosis,and 3patients had progressed to end-stage renal disease by the time they were diagnosed.Two patients received preemptive LT;their estimated glomerular filtration rate was maintained at>120 mL/min/1.73 m2,indicating a better prognosis.Three patients received sequential liver and kidney transplantation.After transplantation,serum and urinary oxalate decreased,and liver function recovered.At the last follow-up,the estimated glomerular filtration rates of the latter 3 patients were 179,52 and 21 mL/min/1.73 m2.CONCLUSION Different transplantation strategies should be adopted for patients based on their renal function stage.Preemptive-LT offers a good therapeutic approach for PH1.展开更多
BACKGROUND Anticoagulation treatment after lower limb surgery is one of the key methods to avoid thrombosis,and low-molecular-weight heparin is the treatment that is most frequently used in clinical practice.But one u...BACKGROUND Anticoagulation treatment after lower limb surgery is one of the key methods to avoid thrombosis,and low-molecular-weight heparin is the treatment that is most frequently used in clinical practice.But one uncommon side effect of lowmolecular-weight heparin is heparin-induced thrombocytopenia(HIT),which can develop into thrombosis if not caught early or managed incorrectly.CASE SUMMARY We present a case of a patient who underwent hip arthroplasty and experienced thrombocytopenia due to HIT on the 9th d following the application of lowmolecular-weight heparin anticoagulation.We did not diagnose HIT in time and applied 1 unit of platelets to the patient,which led to thrombosis.Luckily,the patient recovered following effective and timely surgery and treatment with rivaroxaban.CONCLUSION Patients using low-molecular-weight heparin after lower limb surgery need to have their platelet counts regularly checked.If HIT develops,platelet treatment should be given with caution.展开更多
Purpose: To investigate whether long-term noise exposure affects the cognitive ability of grinders. Methods: Cumulative noise exposure (CNE) and LAeq.8h determination were used to characterize the level of individual ...Purpose: To investigate whether long-term noise exposure affects the cognitive ability of grinders. Methods: Cumulative noise exposure (CNE) and LAeq.8h determination were used to characterize the level of individual noise exposure;the Montreal Cognitive Assessment (MoCA) test (Beijing version) was used to evaluate cognitive function. Results: We compared the basic situation of workers in different groups and individual noise exposure intensity of grinders was monitored. Multiple linear-regression analysis was made and score of MoCA in different group was finally drawn. Conclusion: CNE and total score of MoCA have the relationship of negative correlation (r = -0.303, p < 0.05) which means long-term occupational noise exposure can affect the cognitive ability of grinders.展开更多
The objective of this study was to retrieve daily composite soil moisture by jointly using brightness temperature observations from multiple operating satellites for near real-time application with better coverage and...The objective of this study was to retrieve daily composite soil moisture by jointly using brightness temperature observations from multiple operating satellites for near real-time application with better coverage and higher accuracy.Our approach was to first apply the single-channel brightness radiometric algorithm to estimate soil moisture from the respective brightness temperature observations of the SMAP,SMOS,AMSR2,FY3B,and FY3C satellites on the same day and then produce a daily composite dataset by averaging the individual satellite-retrieved soil moisture.We further evaluated our product,the official soil moisture products of the five satellites,and the ensemble mean (i.e.,arithmetic mean) of the five official satellite soil moisture products against ground observations from two networks in Central Tibet and Anhui Province,China.The results show that our product outperforms the individual released products of the five satellites and their ensemble means in the two validation areas.The root mean square error (RMSE ) values of our product were 0.06 and 0.09 m3/m3 in Central Tibet and Anhui Province,respectively.Relative to the ensemble mean of the five satellite products,our product improves the accuracy by 9.1% and 57.7% in Central Tibet and Anhui Province,respectively.This demonstrates that jointly using brightness temperature observations from multiple satellites to retrieve soil moisture not only improves the spatial coverage of daily observations but also produces better daily composite products.展开更多
We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino ...We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino graft was implanted during each stage. During the firststage, an auxiliary domino liver transplantation was conducted using a domino graft from a 4-year-old female child with Wilson's disease. After removing the right lobe of the FAP patient's liver, the graft was rotated 90 degrees counterclockwise and placed along the right side of the inferior vena cava(IVC). The orifices of the left, middle, and right hepatic veins were reconstructed using an iliac vein patch and then anastomosed to the right side of the IVC. Thirty days later, a second domino liver graft was implanted. The second domino graft was from a 3-yearold female child with an ornithine carbamyl enzyme defect, and it replaced the residual native liver(left lobe). To balance the function and blood flow between the two grafts, a percutaneous transcatheter selective portal vein embolization was performed, and "the left portal vein" of the first graft was blocked 9 mo after the second transplantation. The liver function indices, blood ammonia, and 24-h urinary copper levels were normal at the end of a 3-year follow-up. These two domino donor grafts from donors with different metabolic disorders restored normal liver function. Our experience demonstrated a new approach for resolving metabolic disorders with domino grafts and utilizing explanted livers from children.展开更多
Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-...Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-5,7,8-trihydroxy-3-(4- hydroxybenzyl)-6-methylchroman-4-one(2),on the basis of spectral analysis.展开更多
BACKGROUND: Congenital biliary atresia is a rare condition characterized by idiopathic dysgenesis of the bile ducts. If untreated, congenital biliary atresia leads to liver cirrhosis, liver failure and premature death...BACKGROUND: Congenital biliary atresia is a rare condition characterized by idiopathic dysgenesis of the bile ducts. If untreated, congenital biliary atresia leads to liver cirrhosis, liver failure and premature death. The present study aimed to evaluate the outcomes of orthotopic liver transplantation in children with biliary atresia. METHOD: We retrospectively analyzed 45 patients with biliary atresia who had undergone orthotopic liver transplantation from September 2006 to August 2012. RESULTS: The median age of the patients was 11.0 months (5-102). Of the 45 patients, 41 were younger than 3 years old. Their median weight was 9.0 kg (4.5-29.0), 34 of the 45 patients were less than 10 kg. Thirty-one patients had undergone Kasai portoenterostomy prior to orthotopic liver transplantation. We performed 30 living donor liver transplants and 15 split liver transplants. Six patients died during a follow-up. The median follow-up time of surviving patients was 11.4 months (1.4-73.7). The overall 1-, 2- and 3-year survival rates were 88.9%, 84.4% and 84.4%, respectively. CONCLUSION: With advances in surgical techniques and management, children with biliary atresia after liver transplantation can achieve satisfactory survival in China, although there remains a high risk of complications in the early postoperative period.展开更多
Both lyophilization and electrospinning are commonly used to make chitosan scaffolds.However,it remains unknown which method is better for cell growth.In this study,we established the following groups:(1)lyophilizatio...Both lyophilization and electrospinning are commonly used to make chitosan scaffolds.However,it remains unknown which method is better for cell growth.In this study,we established the following groups:(1)lyophilization group-chitosan scaffolds were prepared by lyophilization method and seeded with Schwann cells from Sprague-Dawley rats aged 3-5 days;(2)electrospinning group-chitosan scaffolds were prepared by electrospinning method and seeded with Schwann cells;(3)control group-Schwann cells were cultured on culture dishes.The growth of Schwann cells was evaluated by immunofluorescence and scanning electron microscopy.Western blot assay was performed to explore the mechanism of Schwann cell growth.Both materials were non-toxic and suitable for the growth of Schwann cells.The pores produced by electrospinning were much smaller than those produced by lyophilization.The proliferation rate and adhesion rate of Schwann cells in the electrospinning group were higher than those in the lyophilization group.Schwann cells cultured on electrospinning scaffolds formed a Bungner band-like structure,and a much greater amount of brain-derived neurotrophic factor was secreted,which can promote the growth of neurons.Our findings show that the chitosan scaffold prepared by the electrospinning method has a nanofiber structure that provides an extracellular matrix that is more favorable for cell-cell interactions.The electrospinning method is more suitable for nerve regeneration than the lyophilization method.This research was approved by the Medical Ethical Committee of Dalian Medical University(approval No.AEE1-2016-045)on March 3,2016.展开更多
Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and puriifcation of Schwann cells are compl...Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and puriifcation of Schwann cells are complicated by contamination with ifbroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. In this study, we collected dorsal root ganglia from neonatal rats from which we obtained highly puriifed Schwann cells using serum-free melanocyte culture medium. The purity of Schwann cells （〉95%） using our method was higher than that using standard medium containing fetal bovine serum. The obtained Schwann cells were implanted into poly（lactic-co-glycolic acid）/chi-tosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were signiifcantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental ifndings suggest that serum-free melanocyte culture medium is conducive to purify Schwann cells and poly（lactic-co-glycolic acid）/chitosan nerve conduits combined with Schwann cells contribute to restore sciatic nerve defects.展开更多
Slurry-phase hydrogenation technology is the frontier topic in the efficient conversion of heavy oils into light fractions around the world.Developing highly active dispersed MoS_(2) catalysts is the major obstacle to...Slurry-phase hydrogenation technology is the frontier topic in the efficient conversion of heavy oils into light fractions around the world.Developing highly active dispersed MoS_(2) catalysts is the major obstacle to realize the industrial application of upgrading heavy oils.In this work,both top-down ball-milling method and bottom-up hydrothermal method were designed to synthesize MoS_(2) catalysts with controllable layer structures.The stacking layers and lateral sizes for micro-scaled MoS_(2) catalysts by ball-milling method can be reduced to their limits and stabilize at 6~8 layers and lateral size of ca.30 nm.The more flexible bottom-up hydrothermal method can construct MoS_(2) catalysts with much smaller lateral sizes and fewer stacking layers,especially,MoS_(2) catalyst fabricated with ammonium tetrathiomolybdate as Mo and S precursor possesses average stacking layers of 2 and lateral size of 5 ~ 10 nm.Polycyclic aromatic hydrocarbons anthracene,phenanthrene and naphthalene were used as model compounds of heavy oils to investigate the catalytic hydrogenation performance of designed MoS_(2) catalysts.The catalytic activities of MoS_(2) catalysts can be well correlated with their stacking layers and lateral size.The edges of top and bottom S-Mo-S atomic layers for MoS_(2) sheets,named rim sites,are positively correlated with the exposure of active sites for catalytic hydrogenation of PAHs.The highest catalytic activity of MoS_(2) catalyst results from its layer structures of 100% rim sites and the smallest lateral size of5 ~ 10 nm,which is beneficial to expose maximum active sites for catalytic hydrogenation reactions.This work can guide us to design the highly active hydrogenation catalysts,and promote the industrial application of upgrading heavy oils.展开更多
Objective:To study the therapeutic mechanisms of pseudolaric acid on allergic contact dermatitis in mice.Methods:A total of 50 BALB/C mice were selected and randomly divided into control group,model group,and treatmen...Objective:To study the therapeutic mechanisms of pseudolaric acid on allergic contact dermatitis in mice.Methods:A total of 50 BALB/C mice were selected and randomly divided into control group,model group,and treatment A,B,C groups with 10 rats in each group.ACD model was established in model group,and treatment A,B,C groups but not in control group.Model group received no treatment,but treatment A,B,C groups were treated with external application of the concentration of 0.1%,0.2% and 0.4% of the pseudolaric acid for the lesions of ear skin.And the weight gain and the swelling degree of the mice' ear were recorded,weight of thymus and spleen were measured.Spleen suspension was prepared to test T lymphocyte and B lymphocyte levels of mice in five groups.Changes in serum IFN-ed through the enzyme linked immunosorbent assay(ELISAγ,IL-4 and IL-10 levels were test).Results:The weight gain of mice in model group were significant lower than those of mice in the control group and the treatment A,B,C groups(P<0.05).Weight gain of mice in treatment A,B groups were significant lower than that of control group(P<0.05),but the difference in weight gain between treatment C group and control group showed no significant difference(P>0.05).The swelling degree and the weight of mice ears in model group were significant higher than those of mice in control group and treatment A,B,C groups(P<0.05).Swelling degree and the weight of mice ears of treatment A,B,C groups were obviously higher than that of control group(P<0.05).The swelling degree and weight of mice' ears in treatment A,B,C groups were decreased with the increase of the drug dosage,but comparison between A,B and C group showed statistically differences(P<0.05).The thymus and spleen index of mice in model group were significant higher than those of the other four groups(P<0.05),among the four groups,thymus and spleen index of treatment A and B group were higher than control group and treatment C group(P<0.05).The stimulation index of T and B cells of mice in model group was significantly higher than the rest four groups(P<0.05).The serum IFN-γ level of mice in control group and treatment A,B and C group was obviously lower than that of mice in model group(P<0.05).The serum IFN-γ level of mice in treatment A,B and C group were decreased with the increasing of the drug dosage,and the level of C group was obviously lower than that of A and B group(P<0.05).Conclusion:The pseudolaric acid has anti-inflammation and immune adjustment the effects showing a remarkable therapeutic effects for the ACD mice.展开更多
基金supported by the Science and Technology Development Fund of Macao SAR(FDCT0128/2022/A,0020/2023/RIB1,0111/2023/AFJ,005/2022/ALC)the Shandong Natural Science Foundation of China(ZR2020MA004)+2 种基金the National Natural Science Foundation of China(12071272)the MYRG 2018-00168-FSTZhejiang Provincial Natural Science Foundation of China(LQ23A010014).
文摘This study introduces a pre-orthogonal adaptive Fourier decomposition(POAFD)to obtain approximations and numerical solutions to the fractional Laplacian initial value problem and the extension problem of Caffarelli and Silvestre(generalized Poisson equation).As a first step,the method expands the initial data function into a sparse series of the fundamental solutions with fast convergence,and,as a second step,makes use of the semigroup or the reproducing kernel property of each of the expanding entries.Experiments show the effectiveness and efficiency of the proposed series solutions.
基金supported jointly by the GAS project (Ref: NE/H001085/1)a China 863 Project (No.2009AA12Z317)+2 种基金supported by the Natural Environmental Research Council (NERC) through the National Center of Earth Observation (NCEO) of which the Center for the Observation and Modelling of Earthquakesfunded by a general project of National Natural Science Foundation of China (NS- FC) (No. 40902081)a key project of the Ministry of Land & Resources, China (No. 1212010914015)
文摘The 2009 M W 7.8 Fiordland （New Zealand） earthquake is the largest to have occurred in New Zealand since the 1931 M W 7.8 Hawke’s Bay earthquake, 1 000 km to the northwest. In this paper two tracks of ALOS PALSAR interferograms （one ascending and one descending） are used to determine fault geometry and slip distribution of this large earthquake. Modeling the event as dislocation in an elastic half-space suggests that the earthquake resulted from slip on a SSW-NNE orientated thrust fault that is associated with the subduction between the Pacific and Australian Plates, with oblique displacement of up to 6.3 m. This finding is consistent with the preliminary studies undertaken by the USGS using seismic data.
文摘Hadoop framework emerged at the right moment when traditional tools were powerless in terms of handling big data. Hadoop Distributed File System (HDFS) which serves as a highly fault-tolerance distributed file system in Hadoop, can improve the throughput of data access effectively. It is very suitable for the application of handling large amounts of datasets. However, Hadoop has the disadvantage that the memory usage rate in NameNode is so high when processing large amounts of small files that it has become the limit of the whole system. In this paper, we propose an approach to optimize the performance of HDFS with small files. The basic idea is to merge small files into a large one whose size is suitable for a block. Furthermore, indexes are built to meet the requirements for fast access to all files in HDFS. Preliminary experiment results show that our approach achieves better performance.
基金financially supported by the grants of the NSFC(32172295,21804028)the key R&D program of Anhui(201904d07020016)+5 种基金the Anhui Provincial NSF(1908085QC121)the Fundamental Research Fund for central university(JZ2019HGTB0068)the China Postdoctoral Science Foundation(2019M652167)the Fund of State Key Lab of Chemo/Biosensing and Chemometrics(Hunan University),the postdoc grant of Anhui(2020B412)Young and Middle-aged Leading Scientists,Engineers and Innovators of the XPCC(2019CB017)China Agriculture Research System-48(CARS-48).
文摘Rapid detection of target foodborne pathogens plays more and more significant roles in food safety,which requires the efficiency,sensitivity,and accuracy.In this research,we proposed a new st rategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture.Th e designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min,resolving the drawbacks of traditional random sampling protocols.After simple and convenient ultrasonication,samples can be directly amplified at 39℃ in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min.Finally,satisfied results have been achieved within 40 min,which greatly improve the efficiency while the accuracy is also guaranteed.Furthermore,all detection steps can be completed under instrument-free conditions.This method will hold great promise for target pathogen detection in the resource-limited district,or for emergency on-site identification.
基金supported by the National Natural Science Foundation of China(32072920)the National Key Research and Development Program of China(2017YFD0501402)+1 种基金the Fundamental Research Funds for the Central Universities(2662022DKPY007)the HZAU-AGIS Cooperation Fund(SZYJY2022024).
文摘Doxycycline hydrochloride and florfenicol combination(DoxHcl&FF)is an effective treatment for respiratory diseases.In the study,our objective Was to evaluate the activity of DoxHcl&FF against Actinobacillus pleuropneumoniae(APP)in porcine pulmonary epithelial lining fluid(PELF)and the optimal dosage scheme to avoid the development of resistance.The DoxHcl&FF Was administered intramuscularly(IM)at 20mg/kg,and the PELF was collected at differ-ent time points.The minimum inhibitory concentration(MIC)and time-mortality curves were also included in the study.Based on the sigmoid Emax equation and dose equations,the study integrated the in vivo pharmacokinetic data of infected pigs and ex vivo pharmacodynamic data to obtain the area under concentration time curve(AUCo-24h)MIC values in PELF and achieve bacteriostatic activity,bactericidal activity and the virtual eradication of bacteria.The study showed that the combination of DoxHcl and FF caused no significant changes in PK parameters.The peak concentration(Cmax)of FF in healthy and diseased pigs was 8.87±0.08 and 8.67±0.07μg/mL,the_AUCo-24h were.172.75±2.52 and 18022±3.13 h-μg/mL,the Cmax of DoxHcl was 7.91±0.09 and 7.99±0.05μg/mL,and the AUCo-24h was 129.96±3.70 h-μg/mL and 169.82±4.38 h-μg/mL.DoxHcl&FF showed strong concentra-tion-dependent tendencies.The bacteriostatic,bactericidal,and elimination activity were calculated as 5.61,18.83 and 32.68 h,and the doses were 1.37(bacteriostatic),4.59(bactericidal)and 7.99(elimination)mg/kg.These findings indicated that the calculated recommended dose could assist in achieving more precise administration,increasing the effectiveness of DoxHcl&FF treatment for APP infections.
文摘BACKGROUND Primary hyperoxaluria type 1(PH1)is a rare autosomal recessive disease stemming from a deficiency in liver-specific alanine-glyoxylate aminotransferase,resulting in increased endogenous oxalate deposition and end-stage renal disease.Organ transplantation is the only effective treatment.However,its approach and timing remain controversial.CASE SUMMARY We retrospectively analyzed 5 patients diagnosed with PH1 from the Liver Transplant Center of the Beijing Friendship Hospital from March 2017 to December 2020.Our cohort included 4 males and 1 female.The median age at onset was 4.0 years(range:1.0-5.0),age at diagnosis was 12.2 years(range:6.7-23.5),age at liver transplantation(LT)was 12.2 years(range:7.0-25.1),and the follow-up time was 26.3 mo(range:12.8-40.1).All patients had delayed diagnosis,and 3patients had progressed to end-stage renal disease by the time they were diagnosed.Two patients received preemptive LT;their estimated glomerular filtration rate was maintained at>120 mL/min/1.73 m2,indicating a better prognosis.Three patients received sequential liver and kidney transplantation.After transplantation,serum and urinary oxalate decreased,and liver function recovered.At the last follow-up,the estimated glomerular filtration rates of the latter 3 patients were 179,52 and 21 mL/min/1.73 m2.CONCLUSION Different transplantation strategies should be adopted for patients based on their renal function stage.Preemptive-LT offers a good therapeutic approach for PH1.
文摘BACKGROUND Anticoagulation treatment after lower limb surgery is one of the key methods to avoid thrombosis,and low-molecular-weight heparin is the treatment that is most frequently used in clinical practice.But one uncommon side effect of lowmolecular-weight heparin is heparin-induced thrombocytopenia(HIT),which can develop into thrombosis if not caught early or managed incorrectly.CASE SUMMARY We present a case of a patient who underwent hip arthroplasty and experienced thrombocytopenia due to HIT on the 9th d following the application of lowmolecular-weight heparin anticoagulation.We did not diagnose HIT in time and applied 1 unit of platelets to the patient,which led to thrombosis.Luckily,the patient recovered following effective and timely surgery and treatment with rivaroxaban.CONCLUSION Patients using low-molecular-weight heparin after lower limb surgery need to have their platelet counts regularly checked.If HIT develops,platelet treatment should be given with caution.
文摘Purpose: To investigate whether long-term noise exposure affects the cognitive ability of grinders. Methods: Cumulative noise exposure (CNE) and LAeq.8h determination were used to characterize the level of individual noise exposure;the Montreal Cognitive Assessment (MoCA) test (Beijing version) was used to evaluate cognitive function. Results: We compared the basic situation of workers in different groups and individual noise exposure intensity of grinders was monitored. Multiple linear-regression analysis was made and score of MoCA in different group was finally drawn. Conclusion: CNE and total score of MoCA have the relationship of negative correlation (r = -0.303, p < 0.05) which means long-term occupational noise exposure can affect the cognitive ability of grinders.
基金supported by the National Key Research and Development Program of China(Grant No.2016YFC0402701)the National Natural Science Foundation of China(Grants No.51879067 and 51579131)+4 种基金the Natural Science Foundation of Jiangsu Province(Grant No.BK20180022)the Six Talent Peaks Project in Jiangsu Province(Grant No.NY-004)the Fundamental Research Funds for the Central Universities of China(Grants No.2018842914 and 2018B04714)the China National Flash Flood Disaster Prevention and Control Project(Grant No.126301001000150068)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX18_0572)
文摘The objective of this study was to retrieve daily composite soil moisture by jointly using brightness temperature observations from multiple operating satellites for near real-time application with better coverage and higher accuracy.Our approach was to first apply the single-channel brightness radiometric algorithm to estimate soil moisture from the respective brightness temperature observations of the SMAP,SMOS,AMSR2,FY3B,and FY3C satellites on the same day and then produce a daily composite dataset by averaging the individual satellite-retrieved soil moisture.We further evaluated our product,the official soil moisture products of the five satellites,and the ensemble mean (i.e.,arithmetic mean) of the five official satellite soil moisture products against ground observations from two networks in Central Tibet and Anhui Province,China.The results show that our product outperforms the individual released products of the five satellites and their ensemble means in the two validation areas.The root mean square error (RMSE ) values of our product were 0.06 and 0.09 m3/m3 in Central Tibet and Anhui Province,respectively.Relative to the ensemble mean of the five satellite products,our product improves the accuracy by 9.1% and 57.7% in Central Tibet and Anhui Province,respectively.This demonstrates that jointly using brightness temperature observations from multiple satellites to retrieve soil moisture not only improves the spatial coverage of daily observations but also produces better daily composite products.
基金Supported by Capital Special Program for Health Research and Development,No.2016-1-2021National Key Technologies R&D Program,No.2015BAI13B09+1 种基金The Training Program of Academic Leaders in Beijing Health System,No.2014-2-002Beijing Municipal Administration of Hospitals Ascent Plan,No.DFL20150101
文摘We report a case of double domino liver transplantation in a 32-year-old woman who was diagnosed with familial amyloid polyneuropathy(FAP) and liver dysfunction. A two-stage surgical plan was designed, and one domino graft was implanted during each stage. During the firststage, an auxiliary domino liver transplantation was conducted using a domino graft from a 4-year-old female child with Wilson's disease. After removing the right lobe of the FAP patient's liver, the graft was rotated 90 degrees counterclockwise and placed along the right side of the inferior vena cava(IVC). The orifices of the left, middle, and right hepatic veins were reconstructed using an iliac vein patch and then anastomosed to the right side of the IVC. Thirty days later, a second domino liver graft was implanted. The second domino graft was from a 3-yearold female child with an ornithine carbamyl enzyme defect, and it replaced the residual native liver(left lobe). To balance the function and blood flow between the two grafts, a percutaneous transcatheter selective portal vein embolization was performed, and "the left portal vein" of the first graft was blocked 9 mo after the second transplantation. The liver function indices, blood ammonia, and 24-h urinary copper levels were normal at the end of a 3-year follow-up. These two domino donor grafts from donors with different metabolic disorders restored normal liver function. Our experience demonstrated a new approach for resolving metabolic disorders with domino grafts and utilizing explanted livers from children.
文摘Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-5,7,8-trihydroxy-3-(4- hydroxybenzyl)-6-methylchroman-4-one(2),on the basis of spectral analysis.
基金supported by a grant from the Tianjin Bureau of Public Health Project (11KG103)
文摘BACKGROUND: Congenital biliary atresia is a rare condition characterized by idiopathic dysgenesis of the bile ducts. If untreated, congenital biliary atresia leads to liver cirrhosis, liver failure and premature death. The present study aimed to evaluate the outcomes of orthotopic liver transplantation in children with biliary atresia. METHOD: We retrospectively analyzed 45 patients with biliary atresia who had undergone orthotopic liver transplantation from September 2006 to August 2012. RESULTS: The median age of the patients was 11.0 months (5-102). Of the 45 patients, 41 were younger than 3 years old. Their median weight was 9.0 kg (4.5-29.0), 34 of the 45 patients were less than 10 kg. Thirty-one patients had undergone Kasai portoenterostomy prior to orthotopic liver transplantation. We performed 30 living donor liver transplants and 15 split liver transplants. Six patients died during a follow-up. The median follow-up time of surviving patients was 11.4 months (1.4-73.7). The overall 1-, 2- and 3-year survival rates were 88.9%, 84.4% and 84.4%, respectively. CONCLUSION: With advances in surgical techniques and management, children with biliary atresia after liver transplantation can achieve satisfactory survival in China, although there remains a high risk of complications in the early postoperative period.
基金supported by the National Natural Science Foundation of China,No.30973060(to WQ).
文摘Both lyophilization and electrospinning are commonly used to make chitosan scaffolds.However,it remains unknown which method is better for cell growth.In this study,we established the following groups:(1)lyophilization group-chitosan scaffolds were prepared by lyophilization method and seeded with Schwann cells from Sprague-Dawley rats aged 3-5 days;(2)electrospinning group-chitosan scaffolds were prepared by electrospinning method and seeded with Schwann cells;(3)control group-Schwann cells were cultured on culture dishes.The growth of Schwann cells was evaluated by immunofluorescence and scanning electron microscopy.Western blot assay was performed to explore the mechanism of Schwann cell growth.Both materials were non-toxic and suitable for the growth of Schwann cells.The pores produced by electrospinning were much smaller than those produced by lyophilization.The proliferation rate and adhesion rate of Schwann cells in the electrospinning group were higher than those in the lyophilization group.Schwann cells cultured on electrospinning scaffolds formed a Bungner band-like structure,and a much greater amount of brain-derived neurotrophic factor was secreted,which can promote the growth of neurons.Our findings show that the chitosan scaffold prepared by the electrospinning method has a nanofiber structure that provides an extracellular matrix that is more favorable for cell-cell interactions.The electrospinning method is more suitable for nerve regeneration than the lyophilization method.This research was approved by the Medical Ethical Committee of Dalian Medical University(approval No.AEE1-2016-045)on March 3,2016.
基金supported by the National Natural Science Foundation of China,No.30973060
文摘Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and puriifcation of Schwann cells are complicated by contamination with ifbroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. In this study, we collected dorsal root ganglia from neonatal rats from which we obtained highly puriifed Schwann cells using serum-free melanocyte culture medium. The purity of Schwann cells （〉95%） using our method was higher than that using standard medium containing fetal bovine serum. The obtained Schwann cells were implanted into poly（lactic-co-glycolic acid）/chi-tosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were signiifcantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental ifndings suggest that serum-free melanocyte culture medium is conducive to purify Schwann cells and poly（lactic-co-glycolic acid）/chitosan nerve conduits combined with Schwann cells contribute to restore sciatic nerve defects.
基金financially supported by the Autonomous Region Key R&D Program of Xin Jiang (2017B02007-1, 2017B02007-2)the High Level Talent Innovation and Entrepreneurship Project of Dalian (2019CT04)。
文摘Slurry-phase hydrogenation technology is the frontier topic in the efficient conversion of heavy oils into light fractions around the world.Developing highly active dispersed MoS_(2) catalysts is the major obstacle to realize the industrial application of upgrading heavy oils.In this work,both top-down ball-milling method and bottom-up hydrothermal method were designed to synthesize MoS_(2) catalysts with controllable layer structures.The stacking layers and lateral sizes for micro-scaled MoS_(2) catalysts by ball-milling method can be reduced to their limits and stabilize at 6~8 layers and lateral size of ca.30 nm.The more flexible bottom-up hydrothermal method can construct MoS_(2) catalysts with much smaller lateral sizes and fewer stacking layers,especially,MoS_(2) catalyst fabricated with ammonium tetrathiomolybdate as Mo and S precursor possesses average stacking layers of 2 and lateral size of 5 ~ 10 nm.Polycyclic aromatic hydrocarbons anthracene,phenanthrene and naphthalene were used as model compounds of heavy oils to investigate the catalytic hydrogenation performance of designed MoS_(2) catalysts.The catalytic activities of MoS_(2) catalysts can be well correlated with their stacking layers and lateral size.The edges of top and bottom S-Mo-S atomic layers for MoS_(2) sheets,named rim sites,are positively correlated with the exposure of active sites for catalytic hydrogenation of PAHs.The highest catalytic activity of MoS_(2) catalyst results from its layer structures of 100% rim sites and the smallest lateral size of5 ~ 10 nm,which is beneficial to expose maximum active sites for catalytic hydrogenation reactions.This work can guide us to design the highly active hydrogenation catalysts,and promote the industrial application of upgrading heavy oils.
基金Supported by the Science and Technology Key Project of Education Department of Henan province (grant No.:15A320046)
文摘Objective:To study the therapeutic mechanisms of pseudolaric acid on allergic contact dermatitis in mice.Methods:A total of 50 BALB/C mice were selected and randomly divided into control group,model group,and treatment A,B,C groups with 10 rats in each group.ACD model was established in model group,and treatment A,B,C groups but not in control group.Model group received no treatment,but treatment A,B,C groups were treated with external application of the concentration of 0.1%,0.2% and 0.4% of the pseudolaric acid for the lesions of ear skin.And the weight gain and the swelling degree of the mice' ear were recorded,weight of thymus and spleen were measured.Spleen suspension was prepared to test T lymphocyte and B lymphocyte levels of mice in five groups.Changes in serum IFN-ed through the enzyme linked immunosorbent assay(ELISAγ,IL-4 and IL-10 levels were test).Results:The weight gain of mice in model group were significant lower than those of mice in the control group and the treatment A,B,C groups(P<0.05).Weight gain of mice in treatment A,B groups were significant lower than that of control group(P<0.05),but the difference in weight gain between treatment C group and control group showed no significant difference(P>0.05).The swelling degree and the weight of mice ears in model group were significant higher than those of mice in control group and treatment A,B,C groups(P<0.05).Swelling degree and the weight of mice ears of treatment A,B,C groups were obviously higher than that of control group(P<0.05).The swelling degree and weight of mice' ears in treatment A,B,C groups were decreased with the increase of the drug dosage,but comparison between A,B and C group showed statistically differences(P<0.05).The thymus and spleen index of mice in model group were significant higher than those of the other four groups(P<0.05),among the four groups,thymus and spleen index of treatment A and B group were higher than control group and treatment C group(P<0.05).The stimulation index of T and B cells of mice in model group was significantly higher than the rest four groups(P<0.05).The serum IFN-γ level of mice in control group and treatment A,B and C group was obviously lower than that of mice in model group(P<0.05).The serum IFN-γ level of mice in treatment A,B and C group were decreased with the increasing of the drug dosage,and the level of C group was obviously lower than that of A and B group(P<0.05).Conclusion:The pseudolaric acid has anti-inflammation and immune adjustment the effects showing a remarkable therapeutic effects for the ACD mice.
