A review of clinical and histological parameters associated with contralateral neck metastases in oral squamous cell carcinoma

Oral squamous cell carcinoma （OSCC） has a high incidence of cervical micrometastases and sometimes metastasizes contralaterally because of the rich lymphatic intercommunications relative to submucosal plexus of oral cavity that freely communicate across the midline, and it can facilitate the spread of neoplastic cells to any area of the neck consequently. Clinical and histopathologic factors continue to provide predictive information to contralateral neck metastases （CLNM） in OSCC, which determine prophylactic and adjuvant treatments for an individual patient. This review describes the predictive value of clinical-histopathologic factors, which relate to primary tumor and cervical lymph nodes, and surgical dissection and adjuvant treatments. In addition, the indications for elective contralateral neck dissection and adjuvant radiotherapy （aRT） and strategies for follow-up are offered, which is strongly focused by clinicians to prevent later CLNM and poor prognosis subsequently.

Impacted Lower Third Molar Fused with a Supernumerary Tooth—Diagnosis and Treatment Planning Using Cone-Beam Computed Tomography

This paper reported a case of fusion between an impacted third molar and a supernumerary tooth, in which a surgical intervention was carried out, with the objective of removing the dental elements. The panoramic radiography was complemented by the Donovan＇s radiographic technique; but because of the proximity of the dental element to the mandibular ramus, it was not possible to have a final fusion diagnosis. Hence, the Cone-Beam Computed Tomography--which provides precise three- dimensional information--was used to determinate the fusion diagnosis and also to help in the surgical planning. In this case report we observed that the periapical, occlusal and panoramic were not able to show details which could only be examined through the cone-beam computed tomo- graphy.

NMR fluid analyzer applying to petroleum industry

Tremendous progress of developing nuclear magnetic resonance(NMR)fluid analyzer has been witnessed in the oil industry for last two decades.This device allows extensive and accurate exploration of fluid properties,such as its hydrogen content,composition,viscosity,hydrogen index(HI),mud filtrate invasion,gas to oil ratio,average velocity,velocity distribution etc.,in the situations of in situ downhole or surface Petro-pipelines.In this review article,we focus on the design principle,manufacturing,implementation,methodology and applications of NMR fluid analyzer to oil and gas industry.A detailed description of the state-of-art NMR fluid analyzers was firstly given to exhibit their respective characteristics.With these experiences on hand,we introduced a series of NMR fluid analyzers designed by us at China University of Petroleum-Beijing with continuous optimizations,in terms of magnet construction,antenna layout,circuit design and operating surroundings.These systems discussed in this article have been demonstrated to achieve multiple NMR parameter acquisition when the fluid is in stationary or flowing state.In the end,a prototype was fabricated and validated considering a vast of engineering influences,such as variable temperatures in a large range,high pressure,limited volume,detection efficiency,etc.A particular emphasis of this paper is to expedite the measurement efficiency of the NMR fluid analyzer to reduce the operation costs.This dilemma can be Figured out by upgrading both pulse sequence and observational mode.For different fluid states,two rapid pulse sequences were proposed to sufficiently obtain the multi-dimensional NMR correlation map.Meanwhile,two observational modes were developed to take full advantage of the polarization time,during which the individual antenna was systematically switched.Another domain of interest in this review concerns the applications of this new tool.For stationary fluids case,accurate identification of fluid properties is of great value for scheme building in oil and gas exploration process.Particularly,it can acquire the fluid content by different NMR responses of different components.In addition,with Bloembergen theory and Stokes-Einstein equation,not only molecular dynamics and composition,but also oil viscosity can be readily evaluated.Moreover,HI information of crude oils will be speculated through partial least square regression.As for flowing fluids case,velocity is a significant parameter to understand the in situ fluid exploitation and therefore evaluate the productivity of certain oil wells or pipelines.Regarding to the unique magnet and antenna designs in our NMR fluid analyzer;this review adopts two distinct methods to obtain flow velocity at a wide rating scale.The first one is a time-of-flight method adaptive in a homogeneous magnetic field,which is suitable in the case of fluid at high flow velocity.The other one relies on the adjacent echo phase difference at a magnetic field with constant gradient,which is preferred for relatively low flow velocity.In the near future,this tool will be tested underground to offer individual fluid velocities by combining both the stationary and flowing analysis methods.

Cold atom clocks and their applications in precision measurements

Cold atom clocks have made remarkable progresses in the last two decades and played critical roles in precision measurements. Primary Cs fountain frequency standards have achieved a total uncertainty of a few parts in 1016, and the best optical clock has reached a type B uncertainty below 10-18. Besides applications in the metrology, navigation, etc.,ultra-stable and ultra-accurate atomic clocks have also become powerful tools in the basic scientific investigations. In this paper, we focus on the recent developments in the high-performance cold atomic clocks which can be used as frequency standards to calibrate atomic time scales. The basic principles, performances, and limitations of fountain clocks and optical clocks based on signal trapped ion or neutral atoms are summarized. Their applications in metrology and other areas are briefly introduced.

Identification of anrF gene, a homology of admM of andrimid biosynthetic gene cluster related to the antagonistic activity of Enterobacter cloacae B8

AIM： To identify the gene （s） related to the antagonistic activity of Enterobacter cloacae B8 and to elucidate its antagonistic mechanism. METHODS： Transposon-mediated mutagenesis and tagging method and cassette PCR-based chromosomal walking method were adopted to isolate the mutant strain （s） of B8 that lost the antagonistic activity and to clone DNA fragments around Tn5 insertion site. Sequence compiling and open reading frame （ORF） finding were done with DNAStar program and homologous sequence and conserved domain searches were performed with BlastN or BlastP programs at www.ncbi.nlm.nih.gov. To verify the gene involved in the antagonistic activity, complementation of a full-length clone of the anrFgene to the mutant B8F strain was used. RESULTS： A 3 321 bp contig around the Tn5 insertion site was obtained and an ORF of 2 634 bp in length designated as anrFgene encoding for a 877 aa polyketide synthase-like protein was identified. It had a homology of 83% at the nucleotide level and 79% ID/87% SIM at the protein level, to the admM gene of Pantoea agglomerans andrimid biosynthetic gene cluster （AY192157）. The Tn5 was inserted at 2 420 bp of the gene corresponding to the COG3319 （the thioesterase domain of type I polyketide synthase） coding region on BSF. The antagonistic activity against Xanthomonas oryzae pv. oryzae was resumed with complementation of the full-length anrFgene to the mutant B8F. CONCLUSION： The anrFgene obtained is related to the antagonistic activity of BS, and the antagonistic substances produced by B8 are andrimid and/or its analogs.

Improvement of the short-term stability of atomic fountain clock with state preparation by two-laser optical pumping

To improve the signal to noise ratio(SNR)and the short-term stability of cesium atomic fountain clocks,the work of two-laser optical pumping is presented theoretically and experimentally.The short-term stability of the NIM6 fountain clock has been improved by preparing more cold atoms in the|F=4,m_(F)=0>clock state with a shortened cycle time.Two π-polarized laser beams overlapped in the horizontal plane have been applied after launching,one is resonant with|F=4>→|F′=4>transition and the other is resonant with|F=3>→|F′=4>transition.With optical pumping,the population accumulated in the|m_(F)=0>clock state is improved from 11%to 63%,and the detection signal is increased by a factor of 4.2,the SNR of the clock transition probability and the short-term stability are also improved accordingly.

Optical state selection process with optical pumping in a cesium atomic fountain clock

We propose and realize a new optical state selection method on a cesium atomic fountain clock by applying a two-laser 3-3'optical pumping configuration to spin polarize atoms.The atoms are prepared in|F=3,mF=0>clock state with optical pumping directly after being launched up,followed by a pushing beam to push away the atoms remaining in the|F=4>state.With a state selection efficiency exceeding 92%,this optical method can substitute the traditional microwave state selection,and helps to develop a more compact physical package.A Ramsey fringe has been achieved with this optical state selection method,and a contrast of 90%is obtained with a full width half maximum of 0.92 Hz.The short-term frequency stability of 6.8×10^(-14)(τ/s)^(-1/2) is acquired.In addition,the number of detected atoms is increased by a factor of 1.7 with the optical state selection.

Rapid progressive vaccine-induced immune thrombotic thrombocytopenia with cerebral venous thrombosis after ChAdOx1 nCoV-19(AZD1222)vaccination:A case report

BACKGROUND Vaccine-induced immune thrombotic thrombocytopenia(VITT)is a rare and potentially life-threatening condition after receiving coronavirus disease vaccines.It is characterized by symptom onset at 5 to 30 d postvaccination,thrombocytopenia,thrombosis,high D-dimer level,and antiplatelet factor 4(anti-PF4)antibody positivity.VITT can progress very fast,requiring urgent management.Only few studies have described its detailed clinical course and imaging changes.We report a typical VITT case in a patient who underwent regular repeated brain imaging examinations.CASE SUMMARY A young woman presented with headaches at 7 d after the ChAdOx1 nCoV-19vaccine(AZD1222)injection.She then showed progressive symptoms of left upper limb clumsiness.Brain computed tomography revealed venous infarction at the right parietal lobe with a hyperacute thrombus in the cortical vein.Two hours later,brain magnetic resonance imaging revealed hemorrhage at the same area.Magnetic resonance venography showed an irregular contour of the right transverse sinus.Laboratory examination revealed a high D-dimer level,thrombocytopenia,and a high titer for anti-PF4 antibodies.She was treated with anticoagulants,intravenous immunoglobulin,and steroids and analgesic agents were administered for pain control.She had a marked improvement on headaches and clumsiness after treatment along with radiological thrombus resolution.During follow-up at the outpatient department,her modified Rankin scale at 90 d was 1.CONCLUSION Clinicians should be alerted whenever patients present with persistent and progressive headaches or focal motor/sensory deficits postvaccination.

Predictors of unfavorable outcome at 90 days in basilar artery occlusion patients

BACKGROUND In a previous study,basilar artery occlusion(BAO)was shown to lead to death or disability in 80%of the patients.The treatment for BAO patients in the acute stage includes thrombolysis and intra-arterial thrombectomy,but not all patients benefit from these treatments.Thus,understanding the predictors of outcome before initiating these treatments is of special interest.AIM To determine the predictors related to the 90-d clinical outcome in patients with BAO in an Asian population.METHODS We performed a retrospective case review of patients admitted to a tertiary stroke center between 2015 and 2019.We used the international classification of diseases-10 criteria to identify cases of posterior circulation stroke.A neurologist reviewed every case,and patients fulfilling the criteria defined in the Basilar Artery International Cooperation Study were included.We then analyzed the patients’characteristics and factors related to the 90-d outcome.RESULTS We identified a total of 99 patients as real BAO cases.Of these patients,33(33.3%)had a favorable outcome at 90 d(modified Rankin Scale:0–3).Moreover,72 patients received intra-arterial thrombectomy,while 13 patients received intravenous tissue-type plasminogen activator treatment.We observed a favorable outcome in 33.3%of the cases and an unfavorable outcome in 66.7%of the cases.We found that the initial National Institutes of Health Stroke Scale(NIHSS)score and several BAO symptoms,including impaired consciousness,tetraparesis,and pupillary abnormalities,were significantly associated with an unfavorable outcome(P<0.05),while cerebellar symptoms were associated with a favorable outcome(P<0.05).In the receiver operating characteristic(ROC)analysis,the areas under the ROC curve of initial NIHSS score,impaired consciousness,tetraparesis,cerebellar symptoms,and pupillary abnormalities were 0.836,0.644,0.727,0.614,and 0.614,respectively.Initial NIHSS score showed a higher AUROC(0.836)compared to BAO symptoms.CONCLUSION The most important predictor of an unfavorable outcome was the initial NIHSS score.BAO symptoms,including tetraparesis,impaired consciousness,and pupillary abnormality were also related to an unfavorable outcome.