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“抽取共性–讲透典型–衍生个性”,构建晶体结构知识点图谱 被引量:1
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作者 王渭娜 刘峰毅 王文亮 《大学化学》 CAS 2024年第3期36-42,共7页
在结构化学教学中,晶体结构的多样性、复杂性和抽象性使教学难度增加,本文介绍了笔者在教学实践中根据晶体结构的对称性,以具体–抽象–具体主线,以“抽取共性–讲透典型–衍生个性”为核心,构建晶体结构知识点图谱的拓展教学方法。具... 在结构化学教学中,晶体结构的多样性、复杂性和抽象性使教学难度增加,本文介绍了笔者在教学实践中根据晶体结构的对称性,以具体–抽象–具体主线,以“抽取共性–讲透典型–衍生个性”为核心,构建晶体结构知识点图谱的拓展教学方法。具体做法是以等径圆球密堆积及其空隙类型/分布作为共性的出发点,通过填隙模型得到典型晶体的结构,通过取代/添加/去除/衍生等方式建立具有多样性的具体物质的晶体结构,形成直观和有脉络关系的知识图谱。可以加深学生理解晶体结构教学中的共性与个性,达到知识理解与迁移运用的目的。 展开更多
关键词 晶体结构 知识图谱 共性 个性
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“薛定谔方程–近似模型–核心概念–简单应用”——构建原子/分子结构教学内容的逻辑框架与知识点图谱
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作者 王文亮 王渭娜 +3 位作者 王素凡 盛天 周涛 魏南 《大学化学》 CAS 2024年第8期338-343,共6页
针对结构化学课程知识点繁多和概念碎片化、学生难以形成学科框架和完整知识体系的困境,本文提出以“薛定谔方程–近似模型–核心概念–简单应用”为主线,以一维势箱自由粒子模型、单电子类H体系、多电子原子、H_(2)^(+)分子离子、H_(2... 针对结构化学课程知识点繁多和概念碎片化、学生难以形成学科框架和完整知识体系的困境,本文提出以“薛定谔方程–近似模型–核心概念–简单应用”为主线,以一维势箱自由粒子模型、单电子类H体系、多电子原子、H_(2)^(+)分子离子、H_(2)分子、多原子分子等“六条分支”来构建学科的逻辑框架与知识点图谱。使碎片化的知识重构关联,形成完整的知识链,帮助学生理解重要概念的逻辑关系、产生脉络及相互联系,并且为学生提供知识迁移的情境,达到知识理解与运用的目的。 展开更多
关键词 原子结构 分子结构 概念碎片化 结构化重构 知识点图谱
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AJUBA调控MST1 YAP1和TAZ因子促进食管鳞状细胞癌细胞体外增殖迁移和侵袭 被引量:5
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作者 苏丽萍 季敏 +5 位作者 路子扬 刘丽 王维娜 苗娜 张巍 蒲红伟 《中国肿瘤临床》 CAS CSCD 北大核心 2022年第7期331-337,共7页
目的:探讨AJUBA在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞中表达以及是否通过调控MST1、YAP1和TAZ因子影响细胞增殖、侵袭和迁移。方法:采用Western blot法检测AJUBA蛋白在食管癌细胞系KYSE30、KYSE150与KYSE450... 目的:探讨AJUBA在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞中表达以及是否通过调控MST1、YAP1和TAZ因子影响细胞增殖、侵袭和迁移。方法:采用Western blot法检测AJUBA蛋白在食管癌细胞系KYSE30、KYSE150与KYSE450中的表达水平,构建shRNA干扰载体AJUBA转染至KYSE150食管癌细胞系;通过体外克隆形成实验、流式细胞术、划痕实验和Transwell实验探究AJUBA对KYSE150细胞的增殖、周期、迁移和侵袭能力的影响。采用RT-PCR和Western blot检测MST1、YAP1和TAZ mRNA和蛋白的表达;核质分离实验检测AJUBA、YAP1和TAZ蛋白表达情况。结果:稳定干扰AJUBA基因后,平板克隆实验结果显示,与阴性对照组相比,shAJUBA组的细胞克隆形成数量明显减少,差异具有统计学意义(P<0.001);流式细胞周期实验结果显示,shAJUBA组中的细胞阻滞于G0/G1期,G2/M期与S期细胞显著减少(P<0.05);划痕实验和Transwell侵袭实验结果显示,与AJUBA空染组相比,shAJUBA组细胞迁移能力和侵袭能力均明显减弱(P<0.001);RT-PCR与Western blot实验结果显示,shAJUBA组细胞中的MST1、YAP1、TAZmRNA与蛋白表达水平均显著降低,差异具有统计学意义(P<0.05)。核质分离实验结果提示AJUBA蛋白在正常食管鳞状上皮细胞株(SHEE)细胞核中表达,而在KYSE150细胞胞质与胞核中表达;YAP1和TAZ蛋白在SHEE细胞中不表达,在KYSE150细胞质与细胞核中表达,上述结果差异均具有统计学意义(均P<0.05)。结论:AJUBA促进食管癌细胞的增殖、侵袭与迁移,可能与激活MST1、YAP1、TAZ因子的表达而影响食管鳞状细胞癌进展有关。 展开更多
关键词 食管鳞状细胞癌 AJUBA蛋白 Hippo信号通路 增殖 侵袭
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结构化学课程教学中融入思政元素的探索 被引量:13
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作者 王渭娜 刘峰毅 王文亮 《大学化学》 CAS 2022年第1期83-88,共6页
在高等学校专业课程教学中融入思政教育教学的理念,是以"立德树人"为教育根本任务的基本要求。结合结构化学课程特点和教学内容,以"培养学生的哲学观和科学观、培养学生民族自豪感和自信心"的目标为切入点,提出结... 在高等学校专业课程教学中融入思政教育教学的理念,是以"立德树人"为教育根本任务的基本要求。结合结构化学课程特点和教学内容,以"培养学生的哲学观和科学观、培养学生民族自豪感和自信心"的目标为切入点,提出结构化学教学中思政元素的融入策略,发挥结构化学课程的"育德功能",实现知识传授和价值引领的有机统一。 展开更多
关键词 结构化学 课程思政 策略
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多参考态从头算方法在结构化学教学中的应用探索:以第二周期同核双原子分子为例 被引量:2
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作者 刘峰毅 钟怡 +1 位作者 王渭娜 王文亮 《大学化学》 CAS 2023年第9期308-312,共5页
采用多参考态波函数方法CASPT2计算了第二周期同核双原子分子的基态平衡核间距和波函数,分析了其波函数中的电子组态贡献。通过在键级计算中引入次要组态的贡献,或采用自然轨道的分数电子占据数,我们合理解释了Be_(2)分子的存在和Be―B... 采用多参考态波函数方法CASPT2计算了第二周期同核双原子分子的基态平衡核间距和波函数,分析了其波函数中的电子组态贡献。通过在键级计算中引入次要组态的贡献,或采用自然轨道的分数电子占据数,我们合理解释了Be_(2)分子的存在和Be―Be间的非零键级,以及B_(2)和C_(2)中化学键键级分别为1–2和2–3的事实。上述结果可作为简单分子轨道理论的补充,共同帮助学生理解双原子分子的结构和成键本质。强调了波函数多组态特征在理解分子结构中的必要性,而且也验证了通过相对简单、高效的多参考态计算实验来辅助教学的可行性。 展开更多
关键词 同核双原子分子 分子轨道理论 多参考态计算 多组态波函数 键级
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介绍一种关于四重映轴S_(4)及四重反轴I_(4)独立性的简洁推导
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作者 王渭娜 刘峰毅 王文亮 《大学化学》 CAS 2023年第5期249-253,共5页
分子点群与晶体学点群是结构化学教学重要内容之一,同时也是教学的一个难点。学生经常提出“为何只有S_(4)轴及I_(4)轴是独立的,而其余映轴及反轴均是非独立的;在描述分子对称性时为何选用映轴,而描述晶体对称性时则选用反轴”等问题。... 分子点群与晶体学点群是结构化学教学重要内容之一,同时也是教学的一个难点。学生经常提出“为何只有S_(4)轴及I_(4)轴是独立的,而其余映轴及反轴均是非独立的;在描述分子对称性时为何选用映轴,而描述晶体对称性时则选用反轴”等问题。本文从循环群的生成元出发,得到映轴群S_(n)及反轴群I_(n)的对称操作群元素。根据映轴是否可以用其他对称元素代替,或对称操作群是否可以表示为两个子群的直积的性质,推导出当n≤6时只有S_(4)及I_(4)是独立的结论。这种推导方式避免了图形说明繁琐和抽象,有利于学生从定义推导上理解对称元素的独立性,便于教学中推广。 展开更多
关键词 对称操作群 四重映轴 四重反轴 独立性
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Simple Method for Preparing Glucose Biosensor Based on Glucose Oxidase in Nanocomposite Material of Single-Wall Carbon Nanotubes/Ionic Liquid
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作者 weina wang Guang Yin +1 位作者 Xiuju Ma Jun Wan 《Journal of Analytical Sciences, Methods and Instrumentation》 2012年第2期54-59,共6页
Based on electric conductivity and wide potential window of ionic liquid (IL) and electric property of single-wall car- bon nanotubes (SWCNTs), composite material of IL-SWCNTs was prepared, glucose sensor was built wi... Based on electric conductivity and wide potential window of ionic liquid (IL) and electric property of single-wall car- bon nanotubes (SWCNTs), composite material of IL-SWCNTs was prepared, glucose sensor was built with this mate-rial for immobilizing glucose oxidase (GOx). It showed good response, sensitivity and stability for long time for glu-cose detection. Linear range for the detection of glucose was from 0.5 × 10–6 M to 12 × 10–6 M while detection limit was 6.26 × 10–8 M (S/N = 3). 展开更多
关键词 Biosensor GLUCOSE OXIDASE SINGLE-WALL Carbon NANOTUBES Ionic Liquid GLUCOSE
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Research on value co-creation elements in full-scene intelligent service
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作者 weina wang Hong Zhang Sumeet Gupta 《Data Science and Management》 2022年第2期77-83,共7页
Compared with common intelligent service,full-scene intelligent service has its uniqueness in high integration,synergy,and technological spillover.However,the traditional service or business model theories cannot prec... Compared with common intelligent service,full-scene intelligent service has its uniqueness in high integration,synergy,and technological spillover.However,the traditional service or business model theories cannot precisely elaborate its sociotechnical contextual nature and value creation logic.To fill this knowledge gap,we provide initial insights into the value co-creation logic in full-scene intelligent service by exploring the value co-creation elements using a data-driven text mining approach.We analyzed 171 business reports on the full-scene intelligent service by the topic modeling using the Latent Dirichlet Allocation(LDA).The findings reveal three main clusters:value proposition,participants,and connection platform.This study presents a theoretical framework for a further exploratory case study and quantitative research on full-scene intelligent service.This study also helps small and medium-sized enterprises to explore and exploit value co-creation opportunities. 展开更多
关键词 Full-scene Value co-creation Latent dirichlet allocation(LDA) Text mining Intelligent service
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Effects of irrigation regimes on soil NO_(3)^(-)-N, electrical conductivity and crop yield in plastic greenhouse 被引量:6
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作者 Tingting Chang Yujie Zhang +5 位作者 Zhanyu Zhang Xiaohou Shao weina wang Jie Zhang Xu Yang Huilian Xu 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2019年第1期109-115,共7页
Developing water-saving irrigation regimes has important practical significance not only in alleviating the crucial water shortage,but also in controlling soil salinization for the protected cultivation in eastern Chi... Developing water-saving irrigation regimes has important practical significance not only in alleviating the crucial water shortage,but also in controlling soil salinization for the protected cultivation in eastern China.A field study with six treatments was conducted to evaluate the effects of different irrigation regimes with subdrainage systems on the soil nitrate nitrogen,salinity and moisture,also evaluate the effects on tomato growth,fruit yield and irrigation water use efficiency(IWUE).The treatments were distinguished by three different irrigation amounts of 310 mm,360 mm and 410 mm,and two irrigation frequencies of 7 and 11 times.Results showed that the irrigation amount had significant effects on the soil NO_(3)^(-)-N and electric conductivity(EC).A positive correlation was detected between soil NO_(3)^(-)-N(x)and EC(y)at 0-20 m depth after harvest,with a linear equation of y=0.063x-0.670.Soil volumetric moisture at 0.10 m and 0.20 m depth was increased as the irrigation amount increased.Moreover,a higher amount of irrigation increased the fruit yield but reduced the IWUE of tomato.It was also found that smaller irrigation amounts combined with frequent intervals could increase fruit yield and IWUE.However,the fruit quality of tomato had a significant(p<0.05)negative correlation with irrigation amount.Therefore,the parameters of irrigation regime including the irrigation amount and intervals should be considered comprehensively in order to find a compromise between salinity control and irrigation water use efficiency improvement. 展开更多
关键词 irrigation regimes GREENHOUSE TOMATO soil nitrate nitrogen soil electric conductivity soil salinity water use efficiency
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TWO-PHASE IMAGE SEGMENTATION BY NONCONVEX NONSMOOTH MODELS WITH CONVERGENT ALTERNATING MINIMIZATION ALGORITHMS
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作者 weina wang Nannan Tian Chunlin Wu 《Journal of Computational Mathematics》 SCIE CSCD 2023年第4期588-622,共35页
Two-phase image segmentation is a fundamental task to partition an image into foreground and background.In this paper,two types of nonconvex and nonsmooth regularization models are proposed for basic two-phase segment... Two-phase image segmentation is a fundamental task to partition an image into foreground and background.In this paper,two types of nonconvex and nonsmooth regularization models are proposed for basic two-phase segmentation.They extend the convex regularization on the characteristic function on the image domain to the nonconvex case,which are able to better obtain piecewise constant regions with neat boundaries.By analyzing the proposed non-Lipschitz model,we combine the proximal alternating minimization framework with support shrinkage and linearization strategies to design our algorithm.This leads to two alternating strongly convex subproblems which can be easily solved.Similarly,we present an algorithm without support shrinkage operation for the nonconvex Lipschitz case.Using the Kurdyka-Lojasiewicz property of the objective function,we prove that the limit point of the generated sequence is a critical point of the original nonconvex nonsmooth problem.Numerical experiments and comparisons illustrate the effectiveness of our method in two-phase image segmentation. 展开更多
关键词 Nonconvex nonsmooth regularization Characteristic function Box constraints Support shrinking alternating minimization Kurdyka-Lojasiewicz property Image segmentation
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Extrapolated Smoothing Descent Algorithm for Constrained Nonconvex and Nonsmooth Composite Problems
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作者 Yunmei CHEN Hongcheng LIU weina wang 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2022年第6期1049-1070,共22页
In this paper,the authors propose a novel smoothing descent type algorithm with extrapolation for solving a class of constrained nonsmooth and nonconvex problems,where the nonconvex term is possibly nonsmooth.Their al... In this paper,the authors propose a novel smoothing descent type algorithm with extrapolation for solving a class of constrained nonsmooth and nonconvex problems,where the nonconvex term is possibly nonsmooth.Their algorithm adopts the proximal gradient algorithm with extrapolation and a safe-guarding policy to minimize the smoothed objective function for better practical and theoretical performance.Moreover,the algorithm uses a easily checking rule to update the smoothing parameter to ensure that any accumulation point of the generated sequence is an(afne-scaled)Clarke stationary point of the original nonsmooth and nonconvex problem.Their experimental results indicate the effectiveness of the proposed algorithm. 展开更多
关键词 Constrained nonconvex and nonsmooth optimization Smooth approximation Proximal gradient algorithm with extrapolation Gradient descent algorithm Image reconstruction
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Influence of computation algorithm on the accuracy of rut depth measurement
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作者 Di wang Augusto Cannone Falchetto +3 位作者 Matthias Goeke weina wang Tiantian Li Michael P.Wistuba 《Journal of Traffic and Transportation Engineering(English Edition)》 2017年第2期156-164,共9页
Rutting is one of the dominant pavement distresses, hence, the accuracy of rut depth measurements can have a substantial impact on the maintenance and rehabilitation (M 8: R) strategies and funding allocation. Diff... Rutting is one of the dominant pavement distresses, hence, the accuracy of rut depth measurements can have a substantial impact on the maintenance and rehabilitation (M 8: R) strategies and funding allocation. Different computation algorithms such as straight- edge method and wire line method, which are based on the same raw data, may lead to rut depth estimation which are not always consistent. Therefore, there is an urgent need to assess the impact of algorithm types on the accuracy of rut depth computation. In this paper, a 1B-point-based laser sensor detection technology, commonly accepted in China for rut depth measurements, was used to obtain a database of 85,000 field transverse profiles having three representative rutting shapes with small, medium and high severity rut levels. Based on the reconstruction of real transverse profiles, the consequences from two different algorithms were compared. Results showed that there is a combined effect of rut depth and profile shape on the rut depth computation accuracy. As expected, the dif- ference between the results obtained with the two computation methods increases with deeper rutting sections: when the distress is above 15 mm (severe level), the average dif- ference between the two computation methods is above 1.5 mm, normally, the wire line method provides larger results. The computation suggests that the rutting shapes have a minimal influence on the results. An in-depth analysis showed that the upheaval outside of the wheel path is a dominant shape factor which results in higher computation differences. 展开更多
关键词 Pavement distress Multipoint laser detection Straight-edge rut depth Wire line rut depth Rutting shape Rut depth magnitude
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