Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system diseases.The neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types involved.Voltage-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related diseases.However,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain unclear.In this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from mice.We found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 expression.Increased VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical neurons.These findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological conditions.Thus,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

Effect of dry-wet cycles on dynamic properties and microstructures of sandstone:Experiments and modelling

Underground pumped storage power plant(UPSP)is an innovative concept for space recycling of abandoned mines.Its realization requires better understanding of the dynamic performance and durability of reservoir rock.This paper conducted ultrasonic detection,split Hopkinson pressure bar(SHPB)impact,mercury intrusion porosimetry(MIP),and backscatter electron observation(BSE)tests to investigate the dynamical behaviour and microstructure of sandstone with cyclical dry-wet damage.A coupling FEM-DEM model was constructed for reappearing mesoscopic structure damage.The results show that dry-wet cycles decrease the dynamic compressive strength(DCS)with a maximum reduction of 39.40%,the elastic limit strength is reduced from 41.75 to 25.62 MPa.The sieved fragments obtain the highest crack growth rate during the 23rd dry-wet cycle with a predictable life of 25 cycles for each rock particle.The pore fractal features of the macropores and micro-meso pores show great differences between the early and late cycles,which verifies the computational statistics analysis of particle deterioration.The numerical results show that the failure patterns are governed by the strain in pre-peak stage and the shear cracks are dominant.The dry-wet cycles reduce the energy transfer efficiency and lead to the discretization of force chain and crack fields.

Non-destructive buffer enabling near-infrared-transparent inverted inorganic perovskite solar cells toward 1400 h light-soaking stable perovskite/Cu(In,Ga)Se_(2) tandem solar cells

Near-infrared(NIR)transparent inverted all-inorganic perovskite solar cells(PSCs)are excellent top cell candidates in tandem applications.An essential challenge is the replacement of metal contacts with transparent conductive oxide(TCO)electrodes,which requires the introduction of a buffer layer to prevent sputtering damage.In this study,we show that the conventional buffers(i.e.,small organic molecules and atomic layer deposited metal oxides)used for organic-inorganic hybrid perovskites are not applicable to all-inorganic perovskites,due to non-uniform coverage of the vulnerable layers underneath,deterioration upon ion bombardment and moisture induced perovskite phase transition,A thin film of metal oxide nanoparticles by the spin-coating method serves as a non-destructive buffer layer for inorganic PSCs.All-inorganic inverted near-infrared-transparent PSCs deliver a PCE of 17.46%and an average transmittance of 73.7%between 780 and 1200 nm.In combination with an 18.56%Cu(In,Ga)Se_(2) bottom cell,we further demonstrate the first all-inorganic perovskite/CIGS 4-T tandem solar cell with a PCE of 24.75%,which exhibits excellent illumination stability by maintaining 86.7%of its initial efficiency after 1400 h.The non-destructive buffer lays the foundation for efficient and stable NIR-transparent inverted inorganic perovskite solar cells and perovskite-based tandems.

Cannabis sativa: origin and history, glandular trichome development, and cannabinoid biosynthesis

Is Cannabis a boon or bane?Cannabis sativa has long been a versatile crop for fiber extraction(industrial hemp),traditional Chinese medicine(hemp seeds),and recreational drugs(marijuana).Cannabis faced global prohibition in the twentieth century because of the psychoactive properties of △^(9)-tetrahydrocannabinol;however,recently,the perspective has changed with the recognition of additional therapeutic values,particularly the pharmacological potential of cannabidiol.A comprehensive understanding of the underlying mechanism of cannabinoid biosynthesis is necessary to cultivate and promote globally the medicinal application of Cannabis resources.Here,we comprehensively review the historical usage of Cannabis,biosynthesis of trichome-specific cannabinoids,regulatory network of trichome development,and synthetic biology of cannabinoids.This review provides valuable insights into the efficient biosynthesis and green production of cannabinoids,and the development and utilization of novel Cannabis varieties.

Molecular mechanism of fly ash affecting the performance of cemented backfill material

The great challenge of cemented tailings backfill(CTB)is difficult simultaneously maintaining its excellent mechanical properties and low cost.Fly ash(FA)can potentially address this problem and further replace cement in favor of low carbon development.However,its mechanism on CTB with low cement dosage and low Ca system remains unclear.Consequently,this study conducted uniaxial compression,Xray diffraction(XRD),and scanning electron microscopy(SEM)-energy dispersive spectrometer(EDS)tests to investigate the effect of FA dosage on the mechanical property and microstructure of CTB.A molecular model of FA-CSH was constructed to reproduce the molecular structure evolution of CTB with FA based on the test results.The influences of FA dosage and calcium/silica molar ratio(Ca/Si ratio)on the matrix strength and failure model were analyzed to reveal the mechanism of FA on calcium silicate hydrated(C-S-H).The results show that the strength of CTB increases initially and then decreases with FA dosage,and the FA supplement leads to a decrease in Ca(OH)_(2) diffraction intensity and Ca/Si ratio around the FA particles.XRD and SEM-EDS findings show that the Ca/Si ratio of C-S-H decreases with the progression of hydration.The FA-CSH model indicates that FA can reinforce the silica chain of C-S-H to increase the matrix strength.However,this enhancement is weakened by supplementing excessive FA dosage.In addition,the hydrogen bonds among water molecules deteriorate,reducing the matrix strength.A low Ca/Si ratio results in an increase in water molecules and a decrease in the ionic bonds combined with Ca^(2+).The hydrogen bonds among water molecules cannot withstand high stresses,resulting in a reduction in strength.The water absorption of the FA-CSH model is negatively correlated with the FA dosage and Ca/Si ratio.The use of optimal FA dosage and Ca/Si ratio leads to suitable water absorption,which further affects the failure mode of FA-CSH.

Video Conference System in Mixed Reality Using a Hololens

The mixed reality conference system proposed in this paper is a robust,real-time video conference application software that makes up for the simple interaction and lack of immersion and realism of traditional video conference,which realizes the entire process of holographic video conference from client to cloud to the client.This paper mainly focuses on designing and implementing a video conference system based on AI segmentation technology and mixed reality.Several mixed reality conference system components are discussed,including data collection,data transmission,processing,and mixed reality presentation.The data layer is mainly used for data collection,integration,and video and audio codecs.The network layer uses Web-RTC to realize peer-to-peer data communication.The data processing layer is the core part of the system,mainly for human video matting and human-computer interaction,which is the key to realizing mixed reality conferences and improving the interactive experience.The presentation layer explicitly includes the login interface of the mixed reality conference system,the presentation of real-time matting of human subjects,and the presentation objects.With the mixed reality conference system,conference participants in different places can see each other in real-time in their mixed reality scene and share presentation content and 3D models based on mixed reality technology to have a more interactive and immersive experience.

High-performance extended short-wavelength infrared PBn photodetectors based on InAs/GaSb/AlSb superlattices

High performance short-wavelength infrared PBn photodetectors based on InAs/GaSb/AlSb superlattices on GaSb substrate have been demonstrated.At 300 K,the device exhibits a 50%cut-off wavelength of~2.1μm as predicted from the band structure calculation;the device responsivity peaks at 0.85 A/W,corresponding to a quantum efficiency(QE)of 56%for 2.0μm-thick absorption region.The dark current density of 1.03×10^(-3)A/cm^(2)is obtained under 50 mV applied bias.The device exhibits a saturated dark current shot noise limited specific detectivity(D*)of 3.29×1010cm·Hz^(1/2)/W(at a peak responsivity of 2.0μm)under-50 mV applied bias.

The measurement of responsivity of infrared photodetectors using a cavity blackbody

For the measurement of responsivity of an infrared photodetector,the most-used radiation source is a blackbody.In such a measurement system,distance between the blackbody,the photodetector and the aperture diameter are two parameters that contribute most measurement errors.In this work,we describe the configuration of our responsivity measurement system in great detail and present a method to calibrate the distance and aperture diameter.The core of this calibration method is to transfer direct measurements of these two parameters into an extraction procedure by fitting the experiment data to the calculated results.The calibration method is proved experimentally with a commercially extended InGaAs detector at a wide range of blackbody temperature,aperture diameter and distance.Then proof procedures are further extended into a detector fabricated in our laboratory and consistent results were obtained.

Prognostic factors of refractory NSCLC patients receiving anlotinib hydrochloride as the third-or further-line treatment

Objective:Anlotinib hydrochloride is a multitarget tyrosine kinase inhibitor that targets vascular endothelial growth factor receptor,fibroblast growth factor receptor,platelet-derived growth factor receptor,c-Kit,and c-MET;therefore,it exhibits both antitumor and anti-angiogenetic activities.A phase III trial has shown that anlotinib improved progression-free survival(PFS)and overall survival(OS)in patients with advanced non-small cell lung cancer(NSCLC),who presented with progressive disease or intolerance after standard chemotherapy.This study aimed to analyze the characteristics of patients receiving anlotinib treatment to determine the dominant populations who are fit for the treatment.Methods:Data were collected from March 2015 to January 2017 from a randomized,double-blind,placebo-controlled,multicenter,phase III trial of anlotinib(ALTER0303).A total of 437 patients were enrolled and randomly allocated(2:1)to the anlotinib and placebo groups.Kaplan–Meier analysis and log-rank test were performed to compare PFS and OS.Cox proportional hazards model was adopted for multivariate prognostic analysis.Results:Multivariate analysis indicated that high post-therapeutic peripheral blood granulocyte/lymphocyte ratio and elevated alkaline phosphatase levels were independent risk factors for PFS.Meanwhile,elevated thyroid-stimulating hormone,blood glucose,and triglyceride levels;hypertension;and hand–foot syndrome were independent protective factors of PFS.High posttherapeutic peripheral blood granulocyte/lymphocyte ratio,an Eastern Cooperative Oncology Group(ECOG)score≥2,and the sum of the maximal target lesion length at baseline were independent risk factors of OS,and hypertriglyceridemia was an independent protective factor of OS.Conclusions:This study preliminarily explored the possible factors that affected PFS and OS after anlotinib treatment in patients with advanced refractory NSCLC,and the baseline characteristics of the therapeutically dominant populations were then identified.

Influence of flavone extract from cultivated saussurea on learning and memory in a mouse model of Alzheimer's disease A comparison with estradiol benzoate

The present study established a mouse model of Alzheimer＇s disease, and investigated the effects of treatment with flavone extract from artificially cultivated saussurea. A positive control group was treated with estradiol benzoate, and learning and memory ability were examined in the 8-arm radial maze. The learning and recognition ability of mice with Alzheimer＇s disease treated with flavone extract was significantly improved and the number of hippocampal neurons was significantly increased in the flavone-treated and positive control groups compared with the model group. The results indicate that flavone extract from artificially cultivated saussurea can improve learning and memory deficits in mice with Alzheimer＇s disease, exerting effects similar to those of estradiol benzoate.

Ultrasound Tweezers Trigger Single-Cell Mechanical Dynamics for Cell Mechanophenotyping

Cells actively modulate mechanobiological circuitry against external perturbations to stabilize whole cell/tissue physiology.The dynamic adaption of cells to mechanical force is critical for cells to perform vital biological functions,from single cell migration to embryonic development.Dysregulation of such dynamics has been associated with pathophysiological conditions in cardiovascular diseases,cancer,aging,and developmental disorders[1].Therefore,a direct understanding of cell’s biomechanical adaptive/maladaptive behaviors and the trigger factors causing the transformation of healthy adaption to maladaptation can help reveal the regulatory role of single cell mechanosensitive dynamics in the progression of various degenerative diseases and aging.However,current efforts for uncovering fundamental associations between disease and cell architecture have been focusing on'static'measurements of biophysical properties,which is limited by the requirement of large sample sizes to obtain statistically significant data.We therefore developed a single and highly integrated platform with mechanical stimulation and fine spatiotemporal sensing functions to probe the single cell mechanical dynamics at subcellular level to determine cell’s mechanophenotypes in healthy and disease conditions.We developed an integrated micromechanical system composed of an’ultrasound tweezer’stimulator[2]and a PDMS micropillar array [3] cellular force sensor to in situ noninvasively probe and monitor single cell mechanical dynamics.Vascular smooth muscle cells(VSMCs)from healthy mouse and mouse with induced abdominal aorta aneurysm(AAA)were used for cell mechanobiological study.An ultrasound transducer(V312-SM,Olympus)was used to generate ultrasound pulses to excite lipid-encapsulated microbubbles(Targeson)binding to cell membrane through an RGD-integrin linkage to apply a transient nanonewton force to VSMCs seeded on the PDMS micropillar array.PDMS micropillar array was fabricated and functionalized as previously described [3] and acts as the mechanical force sensor in our platform.Upon a 1 HZ and 10-second ultrasound stimulation,calcium influx was clearly detected in both healthy and AAA-VSMCs by using the fluo-4 calcium sensor,suggesting the microbubble-integrin-actin cytoskeleton(CSK)linkage can serve as a mechanosensory to sense the ultrasound stimulation.We then examined how healthy and AAA VSMCs would exhibit adaptions to mechanical stimulation at a global cellular scale.After the onset of a 10-second ultrasound stimulation,control and AAA-VSMCs displayed distinct dynamics of CSK tension within 30 mins,in which the CSK tension of healthy VSMCs increased within the reinforcement period(0-5 min)and restored to their ground state with the relaxation period(5-10 min);yet AAA-VSMCs displayed compromised dynamics of such CSK tension upon calcium influx.Quantitative analysis and theoretical modelling revealed the critical roles of myosin motor contraction,F-actin filament polymerization in regulating cell mechanosensitive dynamics in response to a transient mechanical perturbation.The distinct force and CSK dynamics in healthy and AAA conditions indicates that the force-dependent CSK molecular kinetics is a critical factor governing the distinct mechanosensitive dynamics of cells under pathologically dysfunctional conditions.Our results reveal that the mechanical adaptive process of cells to mechanical stimulus can measure the cellular mechanobiological phenotypes featured in both pathologically healthy and diseased context.We demonstrated that an altered mechanobiological phenotype,i.e.AAA-VSMCs with distinct actomyosin-CSK properties potentiates a mechanical maladaptation that reflects progressive accumulation of cellular damage and dysfunction.This may further reveal the pathogenic contexts and their physical mediators featuring biophysical dysregulation in cardiovascular diseases.

Future research needs for environmental science in China

Environmental science is an interdisciplinary science developed in the process of understanding and solving ecolog-ical and environmental problems.In order to tackle these problems,environmental science research is expected to reveal the source,behavior,fate,exposure,and risks of pollutants in the environment and develop potential solutions to control pollution.It provides the scientific basis for decision-makers to establish environmental and economic poli-cies,and promote concerted efforts for the sustainable development of society.Here,we articulate the development patterns,challenges,and future research needs of environmental science in China based on literature review and expert panel discussion.Environmental science research has evolved significantly in the past decade with an increas-ing diversity of environmental pollutants and health impacts,new technologies and methods,deepening fusion of multiple disciplines,and emerging solutions for pollution control.Its future development relies on the advances in our knowledge on the fate and transport of pollutants,regional environmental processes,ecotoxicological effects,environmental exposure and health effects,environmental analysis and monitoring,source control and reduction,environmental remediation,as well as environmental risk management.For each of these fields,we summarize the significant challenges and highlight the research demands for China.Based on the status quo of China’s environmen-tal science research and future needs,we provide recommendations to promote its future development,including encouraging innovation and interdisciplinary research,providing decision support for national needs,encouraging international collaboration,and improving collaboration mechanisms.

A justice and innovative way ahead of consumption-based emission accounting approach

Carbon emission accounting is an important basis for global climate governance.Based on the consumption-based accounting(CBA)method,the characteristics of carbon flow between national,regional,and product processes could be more clearly reflected.Therefore,CBA is more conducive to clarifying the attribution of responsibilities between producers and consumers,with the principles of fairness and justice.By accounting for carbon emissions in typical countries from 1990 to 2019,we found that the CBA emissions are higher than the production-based accounting(PBA)emissions in major developed countries,while the results are reversed for developing countries.In the past 30 years,the CBA emissions in targeted developed countries generally have shown a downward trend,while in developing countries,they have shown an upward trend.CBA emissions in China have shown a continuous growth trend from 1990 to 2019,but the pace has slowed down significantly over the last decade.Meanwhile,the embodied carbon intensity of China’s exports continues to decline,indicating that China is providing more green and low-carbon products to the world.Taking the PV industry as an example,this study further reveals the contribution of specific product industries to the country’s carbon transfer through product carbon footprint analysis.In order to provide a scientific basis for global mitigation and climate governance,it is urgent to innovate a scientific,practical,and standardized CBA technology system.

Erratum to: A justice and innovative way ahead of consumption-based emission accounting approach

The article“A justice and innovative way ahead of consumption-based emission accounting approach”(Sci China Earth Sci,2024,67:2999–3010)contained errors.The corrections in an erratum do not change or affect the result or conclusion of the paper.

Progress in heavy ion cancer therapy at IMP and future development

Basic research on heavy ion cancer therapy such as radiobiology, medicalphysics, and therapeutic technique has been conducted at the Institute ofModern Physics (IMP), Chinese Academy of Sciences since 1995. Based on theachievements acquired in the basic research and the requirements for a heavyion accelerator for radiotherapy purposes, a dedicated heavy ion therapy facilitynamed Heavy Ion Medical Machine (HIMM) was designed at IMP andconstructed in Wuwei, China. The HIMM facility consists of two electroncyclotron resonance ion sources, one cyclotron as the injector and onesynchrotron as the main accelerator, and four different treatment roomsequipped with passive or active beam delivery systems, and accelerates carbonions up to 400 MeV/u. After the performance inspection of HIMM organized bythe National Medical Device Inspection Center, preclinical tests like cell andanimal radiobiological experiments and dosimetric verification using anthropomorphicphantoms for elucidating the biophysical properties of the carbon ionbeams provided by HIMM were carried out. According to the Chinese medicaldevice regulations, a clinical trial in which 46 tumor patients were recruited andtwo hospitals participated was conducted in the HIMM facility, aiming atevaluating the treatment safety and short-term efficacy of the medical device.The success of the clinical trial helped the HIMM facility be authorized by theChinese government as a class III medical device. In this paper, all the aspectsmentioned above are introduced and discussed, and implications for futureimprovements are also given.

Numerical study on the combustion process in a gas turbine combustor with different reference velocities

The mixing and combustion processes under different reference velocities in a gas turbine combustor were numerically investigated using the Flamelet Generated Manifold(FGM)model based on the Reynolds Averaged Navier-Stokes(RANS)method.The flow and combustion fields show strong self-similarity except on the slow auto-ignition in the mixing layer between fuel-rich product and fresh air upstream of the flame stabilization position.The time-scale analysis was carried out to understand the combustion modes inside the combustor.In general,the residence time of the fuel-mixture is much longer than both the chemical time scale and the mixing time scale.Thus,the combustion properties in each sub-zone were dominated by the mean flow structures.Furthermore,the combustion process exhibits a mixing-controlled feature in total.However,partially premixed combustion still appears on the flame base.Most of the fuel was found to be oxidized in the primary zone and the intermediate zone;however,the slow oxidization reactions also play a non-negligible role on the whole combustion process.Finally,a sketch map on the space of mixture fraction and combustion efficiency was proposed to understand the mixing and oxidization experiences of the fuel mixture.

瑞加诺生负荷心肌灌注显像诊断冠心病的初步临床研究

目的探讨瑞加诺生注射液作为负荷药物行核素心肌灌注显像(MPI)诊断冠心病的有效性及安全性。方法选取2017年9月至2018年10月于山西医科大学第一医院住院的疑似冠心病的42名受试者进行回顾性研究。采用完全随机法将受试者分为2组:瑞加诺生组[男性8名、女性9名,年龄(59.83±7.88)岁]和腺苷组[男性12名、女性13名,年龄(56.32± 7.34)岁],分别行瑞加诺生和腺苷注射液的静息MPI后再行负荷MPI,之后2周内所有受试者均行冠状动脉血管造影(CAG)检查。以CAG结果为标准,分析2组MPI诊断冠心病的效能并观察不良反应。计量资料的组间比较采用两独立样本t检验、配对t检验或方差分析、WilcoxonZ秩和检验;计数资料的组间比较采用McNemar检验或Fisher’s确切概率法;瑞加诺生和腺苷的MPI检查方法的一致性比较采用Kappa检验。结果 2组一般资料、CAG阳性率及狭窄程度的比较,差异均无统计学意义(t=-1.503~1.201,Z=-1.346~0.228,Fisher’s确切概率法,均P> 0.05)。瑞加诺生负荷MPI诊断冠心病的灵敏度为70.00%(7/10)、特异度为87.80%(36/41)、阳性预测值为58.33%(7/12)、阴性预测值为92.31%(36/39)、准确率为84.31%(43/51);腺苷负荷MPI诊断冠心病的灵敏度为71.43%(10/14)、特异度为86.89%(53/61)、阳性预测值为55.56%(10/18)、阴性预测值为92.98%(53/57)、准确率为84.00%(63/75),2种MPI方法诊断效能的各项指标的比较差异均无统计学意义(Fisher’s确切概率法,均P=1.00),其分别与CAG结果比较,均存在中度一致性(κ=0.537、0.525,均P<0.001)。药物不良反应为轻微和暂时的,2组间在脸红、胸痛和胸闷、呼吸困难、头晕和头痛、恶心、心悸等不良反应的比较,差异均无统计学意义(Fisher’s确切概率法,均P>0.05),只有腹部不适的差异有统计学意义(Fisher’s确切概率法,P=0.044)。结论瑞加诺生作为心脏负荷试验药物用于MPI诊断冠心病,与腺苷注射液比较具有相似的有效性及安全性。

Overview of current design and analysis of potential theories for automated fibre placement mechanisms

Automated fibre placement(AFP) systems have successfully intensified the demand for high-quality composite component manufacturing in both the military and civilian fields. One of the main elements of these systems is the AFP mechanism for accomplishing individual fibre delivery,clamp/cut/restart(CCR) and the consolidation process, and it consists of several functional submechanisms presenting strong coupling relationships and motion sequences. This review aims to summarize the development of AFP mechanisms and the associated research achievements and provide insight into the research challenges in promoting innovative design in such mechanisms. The systematic development of AFP systems is reviewed in detail, and subsequently, engineering tendency and the general principle of AFP mechanisms are introduced. Focusing on the mechanism design of AFP sub-mechanisms, including the creel assembly CCR and compaction mechanisms,the mechanical schemes as well as the AFP process parameter control are discussed. To improve system reliability and fully optimise AFP mechanisms, the essential theoretical foundation for AFP mechanisms are provided. It is believed that this attempt will help to change the design and optimisation of similar complete mechanisms. Based on the reviewed research, overall remarks and perspectives are presented to serve as a guide for exploring the possibility of novel easy-to-use and cost-effective integrated AFP applications.

AFM-compatible microfluidic platform for affinity- based capture and nanomechanical characterization of circulating tumor cells

Circulating tumor cells(CTCs)carried by the patient’s bloodstream are known to lead to the metastatic spread of cancer.It is becoming increasingly clear that an understanding of the nanomechanical characteristics of CTCs,such as elasticity and adhesiveness,represents advancements in tracking and monitoring cancer progression and metastasis.In the present work,we describe a combined microfluidic–atomic force microscopy(AFM)platform that uses antibody–antigen capture to routinely isolate and nanomechanically characterize CTCs present in blood samples from prostate cancer patients.We introduce the reversible assembly of a microfluidic device and apply refined and robust chemistry to covalently bond antibodies onto its glass substrate with high density and the desired orientation.As a result,we show that the device can efficiently capture CTCs from patients with localized and metastatic prostate cancer through anti-EpCAM,anti-PSA,and anti-PSMA antibodies,and it is suitable for AFM measurements of captured intact CTCs.When nanomechanically characterized,CTCs originating from metastatic cancer demonstrate decreased elasticity and increased deformability compared to those originating from localized cancer.While the average adhesion of CTCs to the AFM tip surface remained the same in both the groups,there were fewer multiple adhesion events in metastatic CTCs than there were in their counterparts.The developed platform is simple,robust,and reliable and can be useful in the diagnosis and prognosis of prostate cancer as well as other forms of cancer.