Differential expression of serum miR-126,miR-141 and miR-21 as novel biomarkers for early detection of liver metastasis in colorectal cancer

Objective：MicroRNAs （miRNAs） have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer （CRC） has not been reported.Methods：We investigated the expression of metastasis-associated miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b and Let7c in localized,liver-metastatic and other organ-metastatic CRC （OM-CRC）.Expressions of target miRNAs in serum were evaluated in 116 consecutive localized CRC （L-CRC）,72 synchronous liver-metastatic CRC （SLM-CRC） and 36 other OM-CRC by quantitative real-time PCR.Results：Seven of 11 tested miRNAs could be detected from serum.Four miRNAs,miR-126,Let-7a,miR141 and miR-21 were identified as metastasis-associated miRNAs.Compared with L-CRC,significant upregulated expression was observed for miR-141 and miR-21 in SLM-CRC and OM-CRC,down-regulated expression was observed for miR-126 in SLM-CRC and OM-CRC,and up-regulated expression of Let-7a in OM-CRC.The receiver operating characteristic （ROC） curve showed serum miR-126 had a cut-off [log10 relative quantity （log10RQ）=--0.2005] with 77.78％ sensitivity and 68.97％ specificity with an area under curve （AUC） of 0.7564,miR-141 had a cut-off （1og10RQ=-0.2285） with 86.11％ sensitivity and 76.11％ specificity with an AUC of 0.8279,and miR-21 had a cut-off （log10RQ=-0.1310） with 73.61％ sensitivity and 66.38％ specificity with an AUC of 0.7479.Conclusions：We identified liver metastasis-associated miRNAs,suggesting serum miR-126,miR-141 and miR-21 may be novel biomarkers for clinical diagnosis of early stage liver-metastatic CRC.

Application of Multi-team Blood Glucose Management Model in Perioperative Period of General Surgery Patients

Objective:In order to explore the application effect of blood glucose management mode of multiteam cooperation in perioperative period of general surgery patients.Methods:The perioperative blood glucose control of 94 patients undergoing surgical treatment in general surgery from January 2016 to March 2019 was reviewed by Non-synchronous.According to the perioperative blood glucose management model of multi-team cooperation,the patients were divided into intervention group and control group.From January 2016 to January 2018,64 patients who did not implemented the multi-team blood glucose management model were the control group and from February 2018 to March 2019,30 patients who implemented the multi-team blood glucose management model were the intervention group,compared with two group about the differences in perioperative blood glucose.Results:It is no statistically significant about two group in highest and lowest blood sugar levels under fasting stats;during the fluid diet the blood sugar level of the intervention group was lower than control group(P<0.05),and when the patients was in the semifluid or food-feeding period,the highest and lowest blood sugar level is that the intervention group was lower than control group(P<0.05).The time of the blood sugar reaching the standard,the coincidence of complications rate and average hospitalization days in the intervention group were particularly lower than control group(P<0.05).Conclusion:If we adopt multiteam blood glucose management model can better control the perioperative blood glucose of patients undergoing general sugar.

Improved Corrosion Behavior of Biodegradable Mg-4Zn-1Mn Alloy Modified by Sr/F co-doped CaP Micro-arc Oxidation Coatings

The Sr/F co-doped CaP(Sr/F-CaP)coatings were prepared by micro-arc oxidation(MAO)under different voltages to modify the microstructure and corrosion behavior of Mg-4Zn-1Mn alloy.The surface and interface characteristics investigated using scanning electron microscopy(SEM)and energy dispersive X-ray spectrometer(EDS)showed that the MAO coatings displayed uneven crater-like holes and tiny cracks under lower voltage,while they exhibited relatively homogeneous crater-like holes without cracks under higher voltage.The thickness of MAO coatings increased with increasing voltage.The corrosion behavior of Mg-4Zn-1Mn alloy was improved by the MAO coatings.The MAO coatings prepared under 450 V and 500 V voltages possessed the best corrosion resistance with regard to the electrochemical corrosion tests and immersion corrosion tests,respectively.The MAO coatings fabricated under 450-500 V could provide a better corrosion protection effect for the substrate.

惠州地区孕期瑜伽练习与产后抑郁的关系的研究

目的探讨产前瑜伽干预对产后抑郁症发生情况的影响。方法选取2013年6月-2017年6月在本院待产的惠州地区孕妇作为试验研究样本,梳理分析病例资料,挑选出200例符合试验要求的孕妇进行入组试验,瑜伽组95例进行瑜伽运动干预,瑜伽练习自孕期16周开始至分娩结束,对照组105例则仅作常规护理。产后分三个阶段(产后3天、42天、3个月)分别对受试个体的抑郁情况进行量化评分,以爱丁堡产后抑郁量表(EPDS)为参考标准进行抑郁评估。结果瑜伽组在产后三个评价阶段,评价得分情况均低于对照组(P<0.05);瑜伽组的抑郁发生率明显低于对照组(P<0.05)。结论对待产孕妇产前行瑜伽干预,可有效将降低产后抑郁症发生率,临床应用前景好。

补气化瘀合剂配合热敷治疗产后出血的临床体会

目的探讨补气化瘀合剂配合热敷治疗产后出血的临床疗效。方法选取我院2016年2月-2017年7月间收治的产后出血患者90例作为研究对象,按照治疗方法分为两组,观察组45例采用补气化瘀合剂配合热敷治疗,对照组45例单用热敷治疗,比较两组患者的临床治疗效果。结果观察组患者治疗总有效率为97.8%,对照组为80.0%,两组患者临床疗效比较差异明显(P<0.01)。结论对产后出血患者给予补气化瘀合剂配合热敷治疗能有效快速止血,效果明显,值得临床推广使用。

Optimization of shale gas fracturing parameters based on artificial intelligence algorithm

Resource-rich shale gas plays a pivotal role in new energy types.The key to scientifically and efficiently developing shale gas fields is to clarify the main factors that affect the production of shale gas wells.In this paper,according to the shale gas reservoir characteristic of the Fuling marine Longmaxi Formation,a single-well geological model was established using the reservoir numerical simulation software CMG.Then,10,000 different reservoir models were randomly generated for different formation physical parameters,completion parameters,and fracturing parameters using the Monte Carlo method,and these 10,000 models were simulated numerically.The machine learning model uses a dataset of 10,000 different geological,completion,and fracturing parameters as input and 10,000 production curves as output.Multiple machine learning regression methods were used to train and test the dataset,and the optimal method(GBDT algorithm)was selected,and the accuracy R2 of the test set of the GBDT prediction model is 0.96.A fracturing parameter optimization workflow was constructed by combining a production prediction model with a particle swarm optimizer(PSO).The process can quickly optimize the fracturing parameters and predict the production for each time by targeting the cumulative gas production under different geological conditions.The optimized parameters are Fracture Spacing,Fracture Width,Intrinsic Permeability,Fracture Half-length,Langmuir Pressure,and Langmuir Volume.The initial predicted cumulative gas production was 4.59×10^(8)m^(3),which was optimized to 4.90×10^(8) m^(3).The proposed PSO-GBDT proxy model can instantly predict the production of shale gas wells with considerable accuracy,reliability,and efficiency,which is a vital tool for optimizing fracture design.This investigation provides a solid foundation for predicting the production of unconventional gas reservoirs and for parameter optimization.

Effect of Ag on High-Temperature Oxidation Behavior of Mg-6.5Gd-5.6Y-0.1Nd-0.01Ce-0.4Zr Alloy

In this paper,the isothermal oxidation experiments were used to study the effect of Ag on the high-temperature oxidation behavior of Mg-6.5Gd-5.6Y-0.1Nd-0.01Ce-0.4Zr(wt%)alloy oxidized at 350℃,400℃ and 450℃ for 120 h.The results show that the oxidation weight gain of the alloy mainly occurs in the early oxidation stage(0-20 h).This reason attributes to the lack of protective oxide film and the rapid inward diffusion of oxygen through the macroscopic defects of the incomplete oxide film.When dense oxide films such as Y_(2)O_(3),Gd_(2)O_(3),and ZrO2 form,they hinder the inward transport of oxygen ions and improve the high-temperature oxidation resistance of the alloy.In addition,the role of the Ag element at three temperatures is different.The addition of Ag mainly promotes the formation of eutectic phases such as Mg3Gd,Mg24Y5,and Ag2Gd,which reduces the content of Gd and Y elements in the alloy matrix,resulting in a decrease in the diffusion rate of Gd and Y elements during the oxidation process at 350℃ and 400℃,and weakens the oxidation resistance of Ag-containing alloys.However,in the oxidation experiment at 450℃,a large amount of eutectic phase is solid dissolved into the matrix,reducing the difference in element content.At this time,it is detected that the Ag element promoted the outward diffusion of Gd and Y elements,accelerating the formation of the oxide film.The oxidation resistance of Ag-containing alloys is improved.

论作品独创性的判断标准

我国《著作权法》一直未明确“独创性”的概念。在司法实践中,独创性判断标准呈现多样化,同案不同判的现象时有发生。界定独创性并优化其判断标准刻不容缓。认定独创性,应考虑著作权法的目的:明确“独”的规范意义;对“创”的认定,不能单纯考察作品的创新部分在整部作品中的占比,而应对作品类别进行细致区分,考虑涉案表达是否与公有领域的表达有差异。优化独创性判断标准有助于统一司法判决的尺度,确保公正与效率。

Degradation and biological properties of Ca-P contained micro-arc oxidation self-sealing coating on pure magnesium for bone fixation

Poor corrosion resistance is one of the main disadvantages for biodegradable magnesium-based metals,especially applied for bone fixation,where there is a high demand of bio-mechanical strength and stability.Surface coating has been proved as an effective method to control the in vivo degradation.In this study a Ca-P self-sealing micro-arc oxidation(MAO)coating was studied to verify its efficacy and biological properties by in vitro and in vivo tests.It was found that the MAO coating could effectively retard the degradation according to immersion and electrochemical tests as well as 3D reconstruction by X-ray tomography after implantation.The MAO coating exhibited no toxicity and could stimulate the new bone formation.Therefore,the Ca-P self-sealing MAO coating could be a potential candidate for application of biodegradable Mg-based implant in bone fixations.

A unified framework of temporal information expression in geosciences knowledge system

Time is an essential reference system for recording objects,events,and processes in the field of geosciences.There are currently various time references,such as solar calendar,geological time,and regional calendar,to represent the knowledge in different domains and regions,which subsequently entails a time conversion process required to interpret temporal information under different time references.However,the current time conversion method is limited by the application scope of existing time ontologies(e.g.,“Jurassic”is a period in geological ontology,but a point value in calendar ontology)and the reliance on experience in conversion processes.These issues restrict accurate and efficient calculation of temporal information across different time references.To address these issues,this paper proposes a Unified Time Framework(UTF)in the geosciences knowledge system.According to a systematic time element parsing from massive time references,the proposed UTF designs an independent time root node to get rid of irrelevant nodes when accessing different time types and to adapt to the time expression of different geoscience disciplines.Furthermore,this UTF carries out several designs:to ensure the accuracy of time expressions by designing quantitative relationship definitions;to enable time calculations across different time elements by designing unified time nodes and structures,and to link to the required external ontologies by designing adequate interfaces.By comparing the time conversion methods,the experiment proves the UTF greatly supports accurate and efficient calculation of temporal information across different time references in SPARQL queries.Moreover,it shows a higher and more stable performance of temporal information queries than the time conversion method.With the advent of the Big Data era in the geosciences,the UTF can be used more widely to discover new geosciences knowledge across different time references.

Geospatial data ontology:the semantic foundation of geospatial data integration and sharing

Effective integration and wide sharing of geospatial data is an important and basic premise to facilitate the research and applications of geographic information science.However,the semantic heterogeneity of geospatial data is a major problem that significantly hinders geospatial data integration and sharing.Ontologies are regarded as a promising way to solve semantic problems by providing a formalized representation of geographic entities and relationships between them in a manner understandable to machines.Thus,many efforts have been made to explore ontology-based geospatial data integration and sharing.However,there is a lack of a specialized ontology that would provide a unified description for geospatial data.In this paper,with a focus on the characteristics of geospatial data,we propose a unified framework for geospatial data ontology,denoted GeoDataOnt,to establish a semantic foundation for geospatial data integration and sharing.First,we provide a characteristics hierarchy of geospatial data.Next,we analyze the semantic problems for each characteristic of geospatial data.Subsequently,we propose the general framework of GeoDataOnt,targeting these problems according to the characteristics of geospatial data.GeoDataOnt is then divided into multiple modules,and we show a detailed design and implementation for each module.Key limitations and challenges of GeoDataOnt are identified,and broad applications of GeoDataOnt are discussed.

Evaluation of the performance of Ca-deficient hydroxyapatite(CDHA)/MgF_(2)bilayer coating on biodegradable high-purity magnesium in a femoral condyle defect model in rabbits

The two most critical factors in promoting the clinical translation of magnesium(Mg)are reducing its degradation rate and improving its osteogenesis.In this study,a Ca-deficient hydroxyapatite(CDHA)/MgF_(2)bilayer coating was prepared on high-purity magnesium(HP Mg)rods by fluorination and hydrothermal treatment.Scanning electron microscope showed that the thickness of the bilayer coating was 3.78 lm and that the surface morphology was nanoscale.In an in vivo experiment on femoral condyle defects in rabbits,the serum magnesium ion levels of rabbits were always in the normal range after surgery,and the liver and kidney functions were not abnormal,which indicated that the CDHA/MgF_(2)bilayer coating has good biosafety.Micro-CT showed that the CDHA/MgF_(2)bilayer coating significantly reduced the degradation rate of the HP Mg rods and enhanced the promotion of bone formation.Hard tissue sections showed that the CDHA/MgF_(2)bilayer coating gave the bone tissue a tight contact interface with the HP Mg rod and improved the bone mass.Immunohistochemistry showed that the expression of vascular endothelial growth factor and BMP-2 was more obvious.These results confirm that the CDHA/MgF_(2)bilayer coating can improve the properties of HP Mg and provide a basis for the further transformation of HP Mg in the future.It also provides a new reference for the surface modification of magnesiummetal.

翻译与对外传播关系小议

翻译与对外传播之间的关系类似于一种共生关系,翻译的终极目标就是对外传播。而对外传播是否有效,翻译起着非常重要的作用。为了让二者达到理想的效果,我们既要从对外传播的视角来审视翻译并由此提升翻译水平,更要尊重传播规律,让翻译与对外传播协同发展,使世界更好地了解和理解中国和中国人。

Biofunctional magnesium coating of implant materials by physical vapour deposition

The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are required.However,the functions of conventional bioactive coatings are limited.Thus novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue repair.By physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and silicon.It was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy implants.In addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are promising.Mg coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial effects.Meanwhile,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating ability.Mg coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in vivo.Inhibitory effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic patients.In addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg coatings.Thus,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties.These novel multi-functional Mg coatings are expected to significantly enhance the long-term safety of bone implants for the benefit of patients.This paper gives a brief review of studies of the microstructure,degradation behaviours and biofunctions of Mg coatings,and directions for future research are also proposed.