A Study of Health Prescribing for Children with Tourette’s Disorders in Children’s Health Education Specialty Care Clinics

Objective:This study designs and implements the health prescription of children’s health education specialized nursing clinic based on the physical and mental characteristics of children with Tourette’s syndrome.Methods:Applying the evidence-based nursing model and nursing intervention theory based on the survey research by searching China Knowledge Network,Wanfang Database and Wipro Chinese Science and Technology Journal Database,the study collected the literature on the health prescription intervention research in children’s Tourette’s disorders health education in children’s health education of specialized nursing outpatient clinic for review and combined with the specialty characteristics to formulate“Children’s Tourette’s Disorder Health Education Prescription.”Results:Tourette’s syndrome in children is a common pediatric neurological disorder,mainly manifested as involuntary,rapid and repeated muscle twitching and vocal tics,which brings serious psychological pressure and life disturbance to the affected children.Conclusion:For children with Tourette’s syndrome,healthcare professionals should give enough care and understanding to the children and their parents,provide timely and effective health education to them,help them improve their bad behavioral habits,establish correct cognitive attitudes,enhance self-confidence,improve patient compliance,reduce the frequency of recurrence,reduce the incidence of complications and promote children’s physical and mental health development.

Research Progress on Combined Chinese and Western Medicine Treatment of Mycoplasma pneumoniae Pneumonia in Children

With the continuous development of medical technology,combined treatment of Chinese and Western medicine has gradually become a research hotspot.As a common disease in pediatrics,the treatment of Mycoplasma pneumoniae pneumonia(MPP)in children is also being explored and improved.This article summarizes the research progress of combined Chinese and Western medicine treatment of MPP in children in recent years,aiming to provide a useful reference for the combined treatment of MPP in children.The article firstly introduces the etiology and pathogenesis of MPP in children,thereafter briefly introduces the Western anti-infective treatment and traditional Chinese medicine(TCM)diagnosis and treatment of MPP in children,and lastly introduces the methods of combined treatment of TCM and Western medicine in detail.The article points out that the combination of Chinese and Western medicine can give full play to the overall regulation of Chinese medicine and the precise treatment advantages of Western medicine,improve the therapeutic effect,reduce the use of antibiotics,and lower the recurrence rate of the disease,which is worthy of further research and promotion.

饲料油菜压青还田对后作小麦土壤真菌群落的影响

【目的】从真菌群落角度揭示麦后复种饲料油菜作绿肥对土壤的培肥效果。【方法】以常规冬小麦-夏玉米一年两作为对照(CK),采用Illumina Miseq高通量测序技术,探讨麦后复种饲料油菜不同播量(S:小播量;M:中播量;L:大播量)与还田时期(D1:9月10日;D2:9月20日;D3:9月30日)下土壤真菌丰度和群落结构。【结果】与对照相比,随油菜播量增加,还田生物量显著增加,小麦产量先增后减,中播量中期还田产量最高;此外,播量增加,还田时间应提前。饲料油菜还田显著降低了土壤真菌OTU数量和丰富度指数(ACE和Chao1)。真菌群落组成方面,门水平下优势菌群主要有子囊菌门、担子菌门、接合菌门和壶菌门,纲水平下油菜还田相比对照优势真菌所占比例增加。功能预测结果表明,腐生营养型是土壤真菌主要营养类型,占比41.32%-50.72%;且还田后兼性功能营养类群增加至30.05%-40.10%;同时显著提高被孢霉属、枝顶孢属、毛壳菌属、青霉菌属等具有生防功能有益菌的丰度。【结论】在晋南旱地麦区推广饲料油菜还田有利于改善土壤真菌群落结构;本试验条件下,油菜中播量种植,9月20日还田为最优措施。

Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus

Streptomyces produces many valuable and important biomolecules with clinical and pharmaceutical applications.The development of simple and highly efficient gene editing tools for genetic modification of Streptomyces is highly desirable.In this study,we developed a screening system for targeted gene knockout using a uracil auxotrophic host(ΔpyrF)resistant to the highly toxic uracil analog of 5-fluoroorotic acid(5-FOA)converted by PyrF,and a non-replicative vector pKC1132-pyrF carrying the complemented pyrF gene coding for orotidine-5'-phosphate decarboxylase.The pyrF gene acts as a positive selection and counterselection marker for recombinants during genetic modifications.Single-crossover homologous integration mutants were selected on minimal medium without uracil by reintroducing pyrF along with pKC1132-pyrF into the genome of the mutantΔpyrF at the targeted locus.Double-crossover recombinants were generated,from which the pyrF gene,plasmid backbone,and targeted gene were excised through homologous recombination exchange.These recombinants were rapidly screened by the counterselection agent,5-FOA.We demonstrated the feasibility and advantage of using this pyrF-based screening system through deleting the otcR gene,which encodes the cluster-situated regulator that directly activates oxytetracycline biosynthesis in Streptomyces rimosus M4018.This system provides a new genetic tool for investigating the genetic characteristics of Streptomyces species.

Solvent regulation strategy of Co-MOF-74 microflower for supercapacitors

Metal-organic frameworks(MOFs)with porous crystal structures have attracted extensive attention in application of energy storage and conversion,owing to their regularity,porosity,large specific surface area,etc.In this work,Co-MOF-74 microflower has been successfully prepared via a controllable solvent regulation strategy.Through modulating the polarity of the solvent,crystals grow in certain preferred orientation and Co-MOF-74 with various morphologies were obtained.Thereinto,the energy storage performance of Co-MOF-74 microflower was measured in both three-electrode system and asymmetric supercapacitor device(specific capacitance of 164.2 F/g at 0.5 A/g in the three-electrode system and 62.5 F/g at 1 A/g in the asymmetric supercapacitor device).This can be attributed to the preferred crystal orientation resulting in a regular and uniform microflower,which is of great significance to electronic interfacial exchange and ion transfer during electrochemical reactions.