The specific pathogenesis of steroid-induced osteonecrosis of the femoral head(SONFH)is still not fully understood,and there is currently no effective early cure.Understanding the role and mechanism of long noncoding ...The specific pathogenesis of steroid-induced osteonecrosis of the femoral head(SONFH)is still not fully understood,and there is currently no effective early cure.Understanding the role and mechanism of long noncoding RNAs(lnc RNAs)in the pathogenesis of SONFH will help reveal the pathogenesis of SONFH and provide new targets for its early prevention and treatment.In this study,we first confirmed that glucocorticoid(GC)-induced apoptosis of bone microvascular endothelial cells(BMECs)is a pre-event in the pathogenesis and progression of SONFH.Then,we identified a new lnc RNA in BMECs via lnc RNA/m RNA microarray,termed Fosassociated linc RNA ENSRNOT00000088059.1(FAR591).FAR591 is highly expressed during GC-induced BMEC apoptosis and femoral head necrosis.Knockout of FAR591 effectively blocked the GC-induced apoptosis of BMECs,which then alleviated the damage of GCs to the femoral head microcirculation and inhibited the pathogenesis and progression of SONFH.In contrast,overexpression of FAR591 significantly promoted the GC-induced apoptosis of BMECs,which then aggravated the damage of GCs to the femoral head microcirculation and promoted the pathogenesis and progression of SONFH.Mechanistically,GCs activate the glucocorticoid receptor,which translocates to the nucleus and directly acts on the FAR591 gene promoter to induce FAR591 gene overexpression.Subsequently,FAR591 binds to the Fos gene promoter(–245–51)to form a stable RNA:DNA triplet structure and then recruits TATA-box binding protein associated factor 15 and RNA polymerase II to promote Fos expression through transcriptional activation.Fos activates the mitochondrial apoptotic pathway by regulating the expression of Bcl-2 interacting mediator of cell death(Bim)and P53 upregulated modulator of apoptosis(Puma)to mediate GC-induced apoptosis of BMECs,which leads to femoral head microcirculation dysfunction and femoral head necrosis.In conclusion,these results confirm the mechanistic link between lnc RNAs and the pathogenesis of SONFH,which helps reveal the pathogenesis of SONFH and provides a new target for the early prevention and treatment of SONFH.展开更多
It has remained a hard nut for years to segment sonar images of jacket installation environment,most of which are noisy images with inevitable blur after noise reduction.For the purpose of solutions to this problem,a ...It has remained a hard nut for years to segment sonar images of jacket installation environment,most of which are noisy images with inevitable blur after noise reduction.For the purpose of solutions to this problem,a fast segmen-tation algorithm is proposed on the basis of the gray value characteristics of sonar images.This algorithm is endowed with the advantage in no need of segmentation thresholds.To realize this goal,we follow the undermentioned steps:first,calcu-late the gray matrix of the fuzzy image background.After adjusting the gray value,the image is divided into three regions:background region,buffer region and target regions.Afterfiltering,we reset the pixels with gray value lower than 255 to binarize images and eliminate most artifacts.Finally,the remaining noise is removed by morphological processing.The simulation results of several sonar images show that the algorithm can segment the fuzzy sonar images quickly and effectively.Thus,the stable and feasible method is testified.展开更多
Driven by safety issues,environmental concerns,and high costs,rechargeable aqueous zinc-ion batteries(ZIBs)have received increasing attention in recent years owing to their unique advantages.However,the sluggish kinet...Driven by safety issues,environmental concerns,and high costs,rechargeable aqueous zinc-ion batteries(ZIBs)have received increasing attention in recent years owing to their unique advantages.However,the sluggish kinetics of divalent charge Zn^(2+)in the cathode materials caused by the strong electrostatic interaction and their unsatisfactory cycle life hinder the development of ZIBs.Herein,organic cations and Zn^(2+)ions co-pre-inserted vanadium oxide([N(CH_(3))_(4)]_(0.77),Zn_(0.23))V_(8)O_(20)·3.8H_(2)O are reported as the cathode for ultra-stable aqueous ZIBs,in which the weaker electrostatic interactions between Zn^(2+)and organic ion-pinned vanadium oxide can induce the high reversibility of Zn^(2+)insertion and extraction,thereby improving the cycle life.It is demonstrated that([N(CH_(3))_(4)]_(0.77),Zn_(0.23))V_(8)O_(20)·3.8H_(2)O cathodes deliver a discharge capacity of 181 mA h g^(-1)at8 A g^(-1)and ultra-long life span(99.5%capacity retention after 2000 cycles).A reversible Zn^(2+)/H^(+)ions(de)intercalation storage process and pseudocapacitive charge storage are characterized.The weaker interactions between organic ion and Zn^(2+)open a novel avenue for the design of highly reversible cathode materials with long-term cycling stability.展开更多
Rechargeable aqueous zinc ion batteries (ZIBs),with the easy operation,cost effectiveness,and high safety,are emerging candidates for high-energy wearable/portable energy storage systems.Unfortunately,the unsatisfacto...Rechargeable aqueous zinc ion batteries (ZIBs),with the easy operation,cost effectiveness,and high safety,are emerging candidates for high-energy wearable/portable energy storage systems.Unfortunately,the unsatisfactory energy density and undesired long-term cycling performance of the cathode hinder the development of ZIBs.Here,we report the chemical preintercalation of a small amount of calcium ions into V2O5 as the cathode material.The cathode of Ca0.04V2O5·1.74H2O (CVO)was demonstrated to have a high specific capacity of 400 mA h g^-1at the current density of 0.05 A g^-1and 187 mA h g^-1at 10 A g^-1,along with impressive capacity retention (100%capacity retention at 10 A g^-1 for 3,000 cycles).Meanwhile,the CVO//Zn battery exhibits a high energy density of 308 Wh kg^-1and a power density of 467 W kg^-1at 0.5 A g^-1.The superior performance originates from the pinning effect of the calcium ions and the lubricating effect of the structural water.The energy storage mechanism of the CVO cathode was also investigated in detail.The new phase (Zn3(OH)2V2O7·2H2O) generated upon cycling participates in the electrochemical reaction and thus contributes to the excellent electrochemical performance.The small amount of Ca^2+ pre-inserted into the interlayer of V2O5 sheds light on constructing cathodes with high energy density for ZIBs.展开更多
Pre-intercalation of metal ions into vanadium oxide is an effective strategy for optimizing the performance of rechargeable zinc-ion battery(ZIB)cathodes.However,the battery long-lifespan achievement and high-capacity...Pre-intercalation of metal ions into vanadium oxide is an effective strategy for optimizing the performance of rechargeable zinc-ion battery(ZIB)cathodes.However,the battery long-lifespan achievement and high-capacity retention remain a challenge.Increasing the electronic conductivity while simultaneously prompting the cathode diffusion kinetics can improve ZIB electrochemical performance.Herein,N-doped vanadium oxide(N-(Zn,en)VO)via defect engineering is reported as cathode for aqueous ZIBs.Positron annihilation and electron paramagnetic resonance clearly indicate oxygen vacancies in the material.Density functional theory(DFT)calculations show that N-doping and oxygen vacancies concurrently increase the electronic conductivity and accelerate the diffusion kinetics of zinc ions.Moreover,the presence of oxygen vacancies substantially increases the storage sites of zinc ions.Therefore,N-(Zn,en)VO exhibits excellent electrochemical performance,including a peak capacity of 420.5 mA h g^(-1)at 0.05 A g^(-1),a high power density of more than 10000 W kg^(-1)at 65.3 Wh kg^(-1),and a long cycle life at 5 A g^(-1)(4500 cycles without capacity decay).The methodology adopted in our study can be applied to other cathodic materials to improve their performance and extend their practical applications.展开更多
Current stretchable surface electrodes have attracted increasing attention owing to their potential applications in biological signal monitoring, wearable human-machine interfaces（HMIs） and the Internet of Things. T...Current stretchable surface electrodes have attracted increasing attention owing to their potential applications in biological signal monitoring, wearable human-machine interfaces（HMIs） and the Internet of Things. The paper proposed a stretchable HMI based on a surface electromyography（sEMG） electrode with a self-similar serpentine configuration. The sEMG electrode was transfer-printed onto the skin surface conformally to monitor biological signals, followed by signal classification and controlling of a mobile robot. Such electrodes can bear rather large deformation（such as 〉30%） under an appropriate areal coverage. The sEMG electrodes have been used to record electrophysiological signals from different parts of the body with sharp curvature, such as the index finger,back of the neck and face, and they exhibit great potential for HMI in the fields of robotics and healthcare. The electrodes placed onto the two wrists would generate two different signals with the fist clenched and loosened. It is classified to four kinds of signals with a combination of the gestures from the two wrists, that is, four control modes. Experiments demonstrated that the electrodes were successfully used as an HMI to control the motion of a mobile robot remotely.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:82260429,82060397,82260434)the Guizhou Provincial Natural Science Foundation(Grant Nos.:Qiankehebasis[2020]1Y311,Qiankehebasis-ZK[2022]general 399,Qiankehebasis-ZK[2022]general 447)+3 种基金the Science and Technology Foundation of Guizhou Provincial Health Committee(Grant Nos.:gzwkj2021-232,gzwjkj2020-1-130,and gzwkj2021-234)the Start-up Fund for Doctoral Research at the Affiliated Hospital of Guizhou Medical University(gyfybsky-2022-14)the NSFC Cultivation Project of Guizhou Medical University(21NSFCP08)the Discipline Outstanding Reserve Talent Program of Affiliated Hospital of Guizhou Medical University。
文摘The specific pathogenesis of steroid-induced osteonecrosis of the femoral head(SONFH)is still not fully understood,and there is currently no effective early cure.Understanding the role and mechanism of long noncoding RNAs(lnc RNAs)in the pathogenesis of SONFH will help reveal the pathogenesis of SONFH and provide new targets for its early prevention and treatment.In this study,we first confirmed that glucocorticoid(GC)-induced apoptosis of bone microvascular endothelial cells(BMECs)is a pre-event in the pathogenesis and progression of SONFH.Then,we identified a new lnc RNA in BMECs via lnc RNA/m RNA microarray,termed Fosassociated linc RNA ENSRNOT00000088059.1(FAR591).FAR591 is highly expressed during GC-induced BMEC apoptosis and femoral head necrosis.Knockout of FAR591 effectively blocked the GC-induced apoptosis of BMECs,which then alleviated the damage of GCs to the femoral head microcirculation and inhibited the pathogenesis and progression of SONFH.In contrast,overexpression of FAR591 significantly promoted the GC-induced apoptosis of BMECs,which then aggravated the damage of GCs to the femoral head microcirculation and promoted the pathogenesis and progression of SONFH.Mechanistically,GCs activate the glucocorticoid receptor,which translocates to the nucleus and directly acts on the FAR591 gene promoter to induce FAR591 gene overexpression.Subsequently,FAR591 binds to the Fos gene promoter(–245–51)to form a stable RNA:DNA triplet structure and then recruits TATA-box binding protein associated factor 15 and RNA polymerase II to promote Fos expression through transcriptional activation.Fos activates the mitochondrial apoptotic pathway by regulating the expression of Bcl-2 interacting mediator of cell death(Bim)and P53 upregulated modulator of apoptosis(Puma)to mediate GC-induced apoptosis of BMECs,which leads to femoral head microcirculation dysfunction and femoral head necrosis.In conclusion,these results confirm the mechanistic link between lnc RNAs and the pathogenesis of SONFH,which helps reveal the pathogenesis of SONFH and provides a new target for the early prevention and treatment of SONFH.
基金supported by Open Fund Project of China Key Laboratory of Submarine Geoscience(KLSG1802)Science&Technology Project of China Ocean Mineral Resources Research and Development Association(DY135-N1-1-05)Science&Technology Project of Zhoushan city of Zhejiang Province(2019C42271,2019C33205).
文摘It has remained a hard nut for years to segment sonar images of jacket installation environment,most of which are noisy images with inevitable blur after noise reduction.For the purpose of solutions to this problem,a fast segmen-tation algorithm is proposed on the basis of the gray value characteristics of sonar images.This algorithm is endowed with the advantage in no need of segmentation thresholds.To realize this goal,we follow the undermentioned steps:first,calcu-late the gray matrix of the fuzzy image background.After adjusting the gray value,the image is divided into three regions:background region,buffer region and target regions.Afterfiltering,we reset the pixels with gray value lower than 255 to binarize images and eliminate most artifacts.Finally,the remaining noise is removed by morphological processing.The simulation results of several sonar images show that the algorithm can segment the fuzzy sonar images quickly and effectively.Thus,the stable and feasible method is testified.
基金supported by the funding from the National Natural Science Foundation of China(grant nos.51902187,52072224,and 51732007)the Natural Science Foundation of Shandong Province(ZR2018BEM010)+3 种基金the Science Fund for Distinguished Young Scholars of Shandong Province(ZR2019JQ16)the Fundamental Research Funds of Shandong UniversityYoung Elite Scientist Sponsorship Program by CAST(YESS)the support from Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong
文摘Driven by safety issues,environmental concerns,and high costs,rechargeable aqueous zinc-ion batteries(ZIBs)have received increasing attention in recent years owing to their unique advantages.However,the sluggish kinetics of divalent charge Zn^(2+)in the cathode materials caused by the strong electrostatic interaction and their unsatisfactory cycle life hinder the development of ZIBs.Herein,organic cations and Zn^(2+)ions co-pre-inserted vanadium oxide([N(CH_(3))_(4)]_(0.77),Zn_(0.23))V_(8)O_(20)·3.8H_(2)O are reported as the cathode for ultra-stable aqueous ZIBs,in which the weaker electrostatic interactions between Zn^(2+)and organic ion-pinned vanadium oxide can induce the high reversibility of Zn^(2+)insertion and extraction,thereby improving the cycle life.It is demonstrated that([N(CH_(3))_(4)]_(0.77),Zn_(0.23))V_(8)O_(20)·3.8H_(2)O cathodes deliver a discharge capacity of 181 mA h g^(-1)at8 A g^(-1)and ultra-long life span(99.5%capacity retention after 2000 cycles).A reversible Zn^(2+)/H^(+)ions(de)intercalation storage process and pseudocapacitive charge storage are characterized.The weaker interactions between organic ion and Zn^(2+)open a novel avenue for the design of highly reversible cathode materials with long-term cycling stability.
基金supported by the National Natural Science Foundation of China(32088102,31730103,31825003,32050081,and 31870218)the CAS Project for Young Scientists in Basic Research(YSBR-011)+2 种基金the Strategic Priority Research Program “Molecular Mechanism of Plant Growth and Development”of the Chinese Academy of Sciences(XDB27040207)the National Key R&D Program of China(2019YFA0904703 and 2016YFA0500502)the Young Elite Scientists Sponsorship Program by CAST(20202022QNRC001/2/3)。
基金This work was supported by the National Natural Science Foundation of China(51902187,52072224,51732007)the Natural Science Foundation of Shandong Province(ZR2018BEM010)+2 种基金the Science Fund for Distinguished Young Scholars of Shandong Province(ZR2019JQ16)the Fundamental Research Funds of Shandong University,Young Elite Scientist Sponsorship Program by CAST(YESS)the Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong.
文摘Rechargeable aqueous zinc ion batteries (ZIBs),with the easy operation,cost effectiveness,and high safety,are emerging candidates for high-energy wearable/portable energy storage systems.Unfortunately,the unsatisfactory energy density and undesired long-term cycling performance of the cathode hinder the development of ZIBs.Here,we report the chemical preintercalation of a small amount of calcium ions into V2O5 as the cathode material.The cathode of Ca0.04V2O5·1.74H2O (CVO)was demonstrated to have a high specific capacity of 400 mA h g^-1at the current density of 0.05 A g^-1and 187 mA h g^-1at 10 A g^-1,along with impressive capacity retention (100%capacity retention at 10 A g^-1 for 3,000 cycles).Meanwhile,the CVO//Zn battery exhibits a high energy density of 308 Wh kg^-1and a power density of 467 W kg^-1at 0.5 A g^-1.The superior performance originates from the pinning effect of the calcium ions and the lubricating effect of the structural water.The energy storage mechanism of the CVO cathode was also investigated in detail.The new phase (Zn3(OH)2V2O7·2H2O) generated upon cycling participates in the electrochemical reaction and thus contributes to the excellent electrochemical performance.The small amount of Ca^2+ pre-inserted into the interlayer of V2O5 sheds light on constructing cathodes with high energy density for ZIBs.
基金National Natural Science Foundation of China,Grant/Award Numbers:51732007,51902187,52072224Natural Science Foundation of Shandong Province,Grant/Award Numbers:ZR2018BEM010,ZR2020YQ35+1 种基金Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of ShandongYoung Elite Scientists Sponsorship Program,Grant/Award Number:2019QNRC001。
文摘Pre-intercalation of metal ions into vanadium oxide is an effective strategy for optimizing the performance of rechargeable zinc-ion battery(ZIB)cathodes.However,the battery long-lifespan achievement and high-capacity retention remain a challenge.Increasing the electronic conductivity while simultaneously prompting the cathode diffusion kinetics can improve ZIB electrochemical performance.Herein,N-doped vanadium oxide(N-(Zn,en)VO)via defect engineering is reported as cathode for aqueous ZIBs.Positron annihilation and electron paramagnetic resonance clearly indicate oxygen vacancies in the material.Density functional theory(DFT)calculations show that N-doping and oxygen vacancies concurrently increase the electronic conductivity and accelerate the diffusion kinetics of zinc ions.Moreover,the presence of oxygen vacancies substantially increases the storage sites of zinc ions.Therefore,N-(Zn,en)VO exhibits excellent electrochemical performance,including a peak capacity of 420.5 mA h g^(-1)at 0.05 A g^(-1),a high power density of more than 10000 W kg^(-1)at 65.3 Wh kg^(-1),and a long cycle life at 5 A g^(-1)(4500 cycles without capacity decay).The methodology adopted in our study can be applied to other cathodic materials to improve their performance and extend their practical applications.
基金supported by the National Natural Science Foundation of China(Nos.51635007,91323303)
文摘Current stretchable surface electrodes have attracted increasing attention owing to their potential applications in biological signal monitoring, wearable human-machine interfaces（HMIs） and the Internet of Things. The paper proposed a stretchable HMI based on a surface electromyography（sEMG） electrode with a self-similar serpentine configuration. The sEMG electrode was transfer-printed onto the skin surface conformally to monitor biological signals, followed by signal classification and controlling of a mobile robot. Such electrodes can bear rather large deformation（such as 〉30%） under an appropriate areal coverage. The sEMG electrodes have been used to record electrophysiological signals from different parts of the body with sharp curvature, such as the index finger,back of the neck and face, and they exhibit great potential for HMI in the fields of robotics and healthcare. The electrodes placed onto the two wrists would generate two different signals with the fist clenched and loosened. It is classified to four kinds of signals with a combination of the gestures from the two wrists, that is, four control modes. Experiments demonstrated that the electrodes were successfully used as an HMI to control the motion of a mobile robot remotely.
