The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigati...The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River(Hangzhou section).Water samples were collected quarterly from seven sites over a one-year period.Physical and chemical parameters,including dissolved oxygen(DO),water temperature(WT),chemical oxygen demand(COD),nitrite(NO_(2)^(-)),active phosphate(PO_(4)^(3-))and other indices were determined.In this study,theαdiversity,βdiversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing analysis.The spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were discussed.The results showed that a total of 57 phyla were detected in the bacterioplankton community,among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative abundance.The results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang River.Mantel and partial Mantel test showed that environmental variables(Mantel r=0.6739,P<0.0001;partial Mantel r=0.507,P=0.0001)were more important than geographical distance(Mantel r=0.5322,P<0.001;partial Mantel r=0.1563,P=0.001).The distance attenuation model showed that there was significant distance attenuation in all four seasons,and the maximum limit of bacterial community diffusion was found in spring.RDA analysis showed that nine environmental factors,including COD,WT and DO,significantly affected community distribution(P<0.05).This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,shedding light on their ecological roles in the Qiantang River.The information obtained can guide future research on the interactions between bacterioplankton and their environment,enabling the development of effective conservation strategies and sustainable management practices for aquatic ecosystems.展开更多
2023年美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)年会于当地时间2023年6月2日在美国芝加哥举行。本次大会报道了多项乳腺癌领域的重要研究进展,涵盖了早期辅助治疗、晚期解救治疗与转化性研究等,这些研究结果将对...2023年美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)年会于当地时间2023年6月2日在美国芝加哥举行。本次大会报道了多项乳腺癌领域的重要研究进展,涵盖了早期辅助治疗、晚期解救治疗与转化性研究等,这些研究结果将对现今乳腺癌诊疗产生实质性的影响,在一些领域将改变临床指南和实践。本文将介绍大会中乳腺癌相关的研究结果,并对部分研究热点展开讨论。展开更多
目的:动态监测2018—2021年中江县人民医院临床分离的肺炎克雷伯菌(KP)株数及耐药性变迁情况,分析其流行病学情况,评估其耐药性的变化。方法:收集中江县人民医院2018年1月—2021年12月临床分离的非重复KP,采用Micro Scan Walk Away 40P...目的:动态监测2018—2021年中江县人民医院临床分离的肺炎克雷伯菌(KP)株数及耐药性变迁情况,分析其流行病学情况,评估其耐药性的变化。方法:收集中江县人民医院2018年1月—2021年12月临床分离的非重复KP,采用Micro Scan Walk Away 40Plus全自动微生物鉴定及药敏仪进行菌种鉴定和药敏试验,使用WHONET5.6软件对药敏结果进行统计分析。结果:2018年1月—2021年12月,共分离出KP 1848株,其中2018年417株,2019年445株,2020年479株,2021年507株,菌株数呈逐年增加趋势。检出菌株数较多的科室为重症监护室(占13.4%)、胸外神经外科(占11.3%)、呼吸内科(占10.1%)。标本类型分布前3位分别为痰液、尿液和分泌物。从年龄分布来看,>65岁的患者人数最多,7~18岁的患者人数最少。在耐药性方面,KP对头孢类药物的耐药率>24.0%,对厄他培南的耐药率<1.0%,对大部分抗菌药物的耐药率<20.0%。结论:2018—2021年KP对大多数常见抗菌药物的耐药率呈现稳中有降的变化趋势,但仍需持续监测分离株的药物敏感性,以指导临床用药。展开更多
建立了在线液相色谱-二极管阵列检测器-串联质谱技术(LC-DAD-MS/MS)同时测定野生菌中多肽类蘑菇毒素(鹅膏毒肽和鬼笔毒肽)的分析方法。样品经甲醇提取,水稀释后,在碱性条件下用XBridge^(TM) BEH C_(18)柱(150m m×3.0 m m,2.5μm)分...建立了在线液相色谱-二极管阵列检测器-串联质谱技术(LC-DAD-MS/MS)同时测定野生菌中多肽类蘑菇毒素(鹅膏毒肽和鬼笔毒肽)的分析方法。样品经甲醇提取,水稀释后,在碱性条件下用XBridge^(TM) BEH C_(18)柱(150m m×3.0 m m,2.5μm)分离,并先后用DAD和MS/MS检测。使用碱性流动相能够实现5种多肽类蘑菇毒素在15 m in内达到基线分离,同时又能有效抑制质谱加钠峰[M+Na]^+的响应信号,保证检测的稳定性和灵敏度。5种蘑菇毒素在0.05~500 mg/kg范围内线性关系良好,相关系数(r)>0.99;检出限为0.005~0.02 mg/kg。通过实际样品检测,证明该法操作简单、快速,灵敏度高,宽的定量检测线性范围能满足实际样品中蘑菇毒素含量变化大的特点。展开更多
基金financially supported by the Fisheries Species Conservation Program of the Agricultural Department of China (Nos.171821303154051044,17190236)the Natural Science Foundation of Zhejiang Province (No.LQ20C190003)+1 种基金the Natural Science Foundation of Ningbo Municipality (Nos.2019A610421,2019A 610443)the K.C.Wong Magna Fund in Ningbo University。
文摘The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River(Hangzhou section).Water samples were collected quarterly from seven sites over a one-year period.Physical and chemical parameters,including dissolved oxygen(DO),water temperature(WT),chemical oxygen demand(COD),nitrite(NO_(2)^(-)),active phosphate(PO_(4)^(3-))and other indices were determined.In this study,theαdiversity,βdiversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing analysis.The spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were discussed.The results showed that a total of 57 phyla were detected in the bacterioplankton community,among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative abundance.The results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang River.Mantel and partial Mantel test showed that environmental variables(Mantel r=0.6739,P<0.0001;partial Mantel r=0.507,P=0.0001)were more important than geographical distance(Mantel r=0.5322,P<0.001;partial Mantel r=0.1563,P=0.001).The distance attenuation model showed that there was significant distance attenuation in all four seasons,and the maximum limit of bacterial community diffusion was found in spring.RDA analysis showed that nine environmental factors,including COD,WT and DO,significantly affected community distribution(P<0.05).This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,shedding light on their ecological roles in the Qiantang River.The information obtained can guide future research on the interactions between bacterioplankton and their environment,enabling the development of effective conservation strategies and sustainable management practices for aquatic ecosystems.
文摘2023年美国临床肿瘤学会(American Society of Clinical Oncology,ASCO)年会于当地时间2023年6月2日在美国芝加哥举行。本次大会报道了多项乳腺癌领域的重要研究进展,涵盖了早期辅助治疗、晚期解救治疗与转化性研究等,这些研究结果将对现今乳腺癌诊疗产生实质性的影响,在一些领域将改变临床指南和实践。本文将介绍大会中乳腺癌相关的研究结果,并对部分研究热点展开讨论。
文摘建立了在线液相色谱-二极管阵列检测器-串联质谱技术(LC-DAD-MS/MS)同时测定野生菌中多肽类蘑菇毒素(鹅膏毒肽和鬼笔毒肽)的分析方法。样品经甲醇提取,水稀释后,在碱性条件下用XBridge^(TM) BEH C_(18)柱(150m m×3.0 m m,2.5μm)分离,并先后用DAD和MS/MS检测。使用碱性流动相能够实现5种多肽类蘑菇毒素在15 m in内达到基线分离,同时又能有效抑制质谱加钠峰[M+Na]^+的响应信号,保证检测的稳定性和灵敏度。5种蘑菇毒素在0.05~500 mg/kg范围内线性关系良好,相关系数(r)>0.99;检出限为0.005~0.02 mg/kg。通过实际样品检测,证明该法操作简单、快速,灵敏度高,宽的定量检测线性范围能满足实际样品中蘑菇毒素含量变化大的特点。
