The single cell gel electrophoresis （SCGE） technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepa...The single cell gel electrophoresis （SCGE） technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to rn-dinitrobenzene （ m-DNB）, 2,4-dinitrotoluene （ 2,4-DNT）, 2,6-dinitroto-luene（2,6-DNT）, 4-nitrotoluene（4-NT） and 2,4-dinitroaniline（2,4-DNAn） were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different （ P 〈 0. 01 ）. All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration（m-DNB ： 0. 04-25μmol/L; 2,4-DNT, 2,6-DNT and 4-NT： 0. 032-500μmol/L; 2,4-DNAn ：0. 8-20μmol/L）. When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship.展开更多
The IGC 50 (50% inhibitory growth concentration) values of 27 nitrobenzenes were determined for the population growth endpoint of Tetrahymena pyriformis . The toxicity order of the observed compounds was found to be a...The IGC 50 (50% inhibitory growth concentration) values of 27 nitrobenzenes were determined for the population growth endpoint of Tetrahymena pyriformis . The toxicity order of the observed compounds was found to be as follows: dinitro compounds>mononitro compounds, dichloronitrobenzenes>monochloronitrobenzenes, meta substituted nitrobenzenes>ortho /para substituted nitrobenzenes(NT, NPh, NAnis) except for the dinitrobenzenes and nitroanilines(DNB, NAn). Quantitative structure activity relationships(QSARs) were developed with the logarithm of the reciprocal of IGC 50 [lg(IGC 50 ) -1 ] in mole liter as dependent variable and six molecular descriptors lg P , 1 X Ⅴ, I, 1K a, ∑σ - and E LUMO as independent variables. Via multiple regression analysis, one best equation was obtained: lg(IGC 50 ) -1 =3.029+0.860∑ σ -+0.341I n=27, r=0.924, r 2=0.854, s=0.265, f=70.44 , Pr> f =0.000 1 The equation was used to estimate IGC 50 for five analogues.展开更多
Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular...Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.展开更多
We investigate the quantum discord dynamics in a cavity quantum electrodynamics system,which consists of two noninteracting two-level atoms driven by independent optical fields and classical fields,and find that the q...We investigate the quantum discord dynamics in a cavity quantum electrodynamics system,which consists of two noninteracting two-level atoms driven by independent optical fields and classical fields,and find that the quantum discord vanishes only asymptotically although entanglement disappears suddenly during the time evolution in the absence of classical fields.It is shown that the amount of quantum discord can be increased by adjusting the classical driving fields because the increasing degree of the amount of quantum mutual information is greater than classical correlation by applying the classical driving fields.Finally,the influence of the classical driving field on the fidelity of the system is also examined.展开更多
文摘The single cell gel electrophoresis （SCGE） technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to rn-dinitrobenzene （ m-DNB）, 2,4-dinitrotoluene （ 2,4-DNT）, 2,6-dinitroto-luene（2,6-DNT）, 4-nitrotoluene（4-NT） and 2,4-dinitroaniline（2,4-DNAn） were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different （ P 〈 0. 01 ）. All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration（m-DNB ： 0. 04-25μmol/L; 2,4-DNT, 2,6-DNT and 4-NT： 0. 032-500μmol/L; 2,4-DNAn ：0. 8-20μmol/L）. When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship.
基金Supported by the Educational Ministry Foundation of China(No. 0 10 6 3) .Tetrahymena pyriformis B was kindlysupplied by Dr.TiedkeMünster
文摘The IGC 50 (50% inhibitory growth concentration) values of 27 nitrobenzenes were determined for the population growth endpoint of Tetrahymena pyriformis . The toxicity order of the observed compounds was found to be as follows: dinitro compounds>mononitro compounds, dichloronitrobenzenes>monochloronitrobenzenes, meta substituted nitrobenzenes>ortho /para substituted nitrobenzenes(NT, NPh, NAnis) except for the dinitrobenzenes and nitroanilines(DNB, NAn). Quantitative structure activity relationships(QSARs) were developed with the logarithm of the reciprocal of IGC 50 [lg(IGC 50 ) -1 ] in mole liter as dependent variable and six molecular descriptors lg P , 1 X Ⅴ, I, 1K a, ∑σ - and E LUMO as independent variables. Via multiple regression analysis, one best equation was obtained: lg(IGC 50 ) -1 =3.029+0.860∑ σ -+0.341I n=27, r=0.924, r 2=0.854, s=0.265, f=70.44 , Pr> f =0.000 1 The equation was used to estimate IGC 50 for five analogues.
基金Supported by the National Natural Science Foundation of China(No.20877017)the National High Technology Research and Development Program of China (No.2007AA03Z314)
文摘Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.
基金Supported by the National Natural Science Foundation of China under Grant No 10774143.
文摘We investigate the quantum discord dynamics in a cavity quantum electrodynamics system,which consists of two noninteracting two-level atoms driven by independent optical fields and classical fields,and find that the quantum discord vanishes only asymptotically although entanglement disappears suddenly during the time evolution in the absence of classical fields.It is shown that the amount of quantum discord can be increased by adjusting the classical driving fields because the increasing degree of the amount of quantum mutual information is greater than classical correlation by applying the classical driving fields.Finally,the influence of the classical driving field on the fidelity of the system is also examined.
