Background:Calcific aortic valve stenosis(CAVS)is one of the most challenging heart diseases in clinical with rapidly increasing prevalence.However,study of the mecha-nism and treatment of CAVS is hampered by the lack...Background:Calcific aortic valve stenosis(CAVS)is one of the most challenging heart diseases in clinical with rapidly increasing prevalence.However,study of the mecha-nism and treatment of CAVS is hampered by the lack of suitable,robust and efficient models that develop hemodynamically significant stenosis and typical calcium deposi-tion.Here,we aim to establish a mouse model to mimic the development and features of CAVS.Methods:The model was established via aortic valve wire injury(AVWI)combined with vitamin D subcutaneous injected in wild type C57/BL6 mice.Serial transthoracic echocardiography was applied to evaluate aortic jet peak velocity and mean gradi-ent.Histopathological specimens were collected and examined in respect of valve thickening,calcium deposition,collagen accumulation,osteogenic differentiation and inflammation.Results:Serial transthoracic echocardiography revealed that aortic jet peak velocity and mean gradient increased from 7 days post model establishment in a time depend-ent manner and tended to be stable at 28 days.Compared with the sham group,sim-ple AVWI or the vitamin D group,the hybrid model group showed typical pathological features of CAVS,including hemodynamic alterations,increased aortic valve thicken-ing,calcium deposition,collagen accumulation at 28 days.In addition,osteogenic dif-ferentiation,fibrosis and inflammation,which play critical roles in the development of CAVS,were observed in the hybrid model.Conclusions:We established a novel mouse model of CAVS that could be induced efficiently,robustly and economically,and without genetic intervention.It provides a fast track to explore the underlying mechanisms of CAVS and to identify more effec-tive pharmacological targets.展开更多
Since the beginning of grape cultivation in the 1980 s in Wuhan City,the grape industry has shown a steady growth trend. Through the survey of the local grape industry,it found out the regional distribution,scale and ...Since the beginning of grape cultivation in the 1980 s in Wuhan City,the grape industry has shown a steady growth trend. Through the survey of the local grape industry,it found out the regional distribution,scale and benefits of the grape,as well as the variety structure and cultivation management mode. It analyzed main problems in the grape production,including low degree of organization,concentrated maturity,low rate of good fruit,small cultivation scale,and low labor-saving and high-efficiency cultivation technologies. On the basis of the development status,it came up with pertinent recommendations,including optimizing the variety structure,implementing standardized management,strengthening construction of sightseeing parks,promoting integration of primary and tertiary industries,and developing the gardening experience,to promote healthy and sustainable development of the grape industry.展开更多
Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bear...Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bearing period. It is propagated mainly through grafting,and is suitable for popularization and application in the landscape of the Yangtze River basin and north of the Yangtze River.展开更多
Considering the surgical risk stratification for patients with severe calcific aortic stenosis(AS),transcatheter aortic valve replacement(TAVR)is a reliable alternative to surgical aortic valve replacement(SAVR)(Fan e...Considering the surgical risk stratification for patients with severe calcific aortic stenosis(AS),transcatheter aortic valve replacement(TAVR)is a reliable alternative to surgical aortic valve replacement(SAVR)(Fan et al.,2020,2021;Lee et al.,2021).Despite the favorable clinical benefits of TAVR,stroke remains a dreaded perioperative complication(Auffret et al.,2016;Kapadia et al.,2016;Kleiman et al.,2016;Huded et al.,2019).Ischemic overt stroke,identified in 1.4%to 4.3%of patients in TAVR clinical practice,has been associated with prolonged disability and increased mortality(Auffret et al.,2016;Kapadia et al.,2016;Levi et al.,2022).The prevalence of hyperintensity cerebral ischemic lesions detected by diffusion-weighted magnetic resonance imaging(DW-MRI)was reported to be about 80%,which is associated with impaired neurocognitive function and vascular dementia(Vermeer et al.,2003;Barber et al.,2008;Kahlert et al.,2010).展开更多
Aortic stenosis(AS)is a progressive heart valve disease occurring predominantly in older patients.According to a survey in a western country,the prevalence of AS is nearly 6.4%in patients over 75 years old(Carabello a...Aortic stenosis(AS)is a progressive heart valve disease occurring predominantly in older patients.According to a survey in a western country,the prevalence of AS is nearly 6.4%in patients over 75 years old(Carabello and Paulus,2009).Transcatheter aortic valve replacement(TAVR)is an alternative method for AS patients.Previous studies have described how up to 66%of TAVR patients have concomitant baseline kidney dysfunction(Ferro et al.,2015;Gargiulo et al.,2015).The majority of patients can benefit from the TAVR procedure with the recovery of kidney function.展开更多
Transcatheter aortic valve replacement(TAVR)has emerged as a viable treatment option for patients with severe aortic stenosis regardless of its surgical risk stratification(Otto et al.,2021).Aortic angulation is usual...Transcatheter aortic valve replacement(TAVR)has emerged as a viable treatment option for patients with severe aortic stenosis regardless of its surgical risk stratification(Otto et al.,2021).Aortic angulation is usually measured as the angle between the horizon-tai and the aortic annulus planes based on preprocedural multidetector computed tomography(MDCT)(Al-Lamee et al.,2011).Extremely horizontal aorta,defined as an aortic angulation greater than 70°.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:81770252,82030014,82271606 and U22A20267Binjiang Institute of Zhejiang University,Grant/Award Number:ZY202205SMKY001Key Program of Major Science and Technology Projects in Zhejiang Province,Grant/Award Number:2021C03097 and 2022C03063。
文摘Background:Calcific aortic valve stenosis(CAVS)is one of the most challenging heart diseases in clinical with rapidly increasing prevalence.However,study of the mecha-nism and treatment of CAVS is hampered by the lack of suitable,robust and efficient models that develop hemodynamically significant stenosis and typical calcium deposi-tion.Here,we aim to establish a mouse model to mimic the development and features of CAVS.Methods:The model was established via aortic valve wire injury(AVWI)combined with vitamin D subcutaneous injected in wild type C57/BL6 mice.Serial transthoracic echocardiography was applied to evaluate aortic jet peak velocity and mean gradi-ent.Histopathological specimens were collected and examined in respect of valve thickening,calcium deposition,collagen accumulation,osteogenic differentiation and inflammation.Results:Serial transthoracic echocardiography revealed that aortic jet peak velocity and mean gradient increased from 7 days post model establishment in a time depend-ent manner and tended to be stable at 28 days.Compared with the sham group,sim-ple AVWI or the vitamin D group,the hybrid model group showed typical pathological features of CAVS,including hemodynamic alterations,increased aortic valve thicken-ing,calcium deposition,collagen accumulation at 28 days.In addition,osteogenic dif-ferentiation,fibrosis and inflammation,which play critical roles in the development of CAVS,were observed in the hybrid model.Conclusions:We established a novel mouse model of CAVS that could be induced efficiently,robustly and economically,and without genetic intervention.It provides a fast track to explore the underlying mechanisms of CAVS and to identify more effec-tive pharmacological targets.
基金Agricultural Science Innovation Project of Wuhan City"Demonstration and Extension of Grape,Citrus and Other Fruit Tree Facilities Cultivation Technologies and Green Prevention and Control"(CXQYDJ201804)
文摘Since the beginning of grape cultivation in the 1980 s in Wuhan City,the grape industry has shown a steady growth trend. Through the survey of the local grape industry,it found out the regional distribution,scale and benefits of the grape,as well as the variety structure and cultivation management mode. It analyzed main problems in the grape production,including low degree of organization,concentrated maturity,low rate of good fruit,small cultivation scale,and low labor-saving and high-efficiency cultivation technologies. On the basis of the development status,it came up with pertinent recommendations,including optimizing the variety structure,implementing standardized management,strengthening construction of sightseeing parks,promoting integration of primary and tertiary industries,and developing the gardening experience,to promote healthy and sustainable development of the grape industry.
基金Supported by Innovation Projects of Wuhan Academy of Agricultural Sciences(Cx201317Cxfzx201506Cxtd201507)
文摘Fly fire is an elite variety of North American Begonia（ Malus micromalus）,which is characterized with dark red flowers,red young leaves,red peel,red bark,large flower amount,long flowering period and long fruit bearing period. It is propagated mainly through grafting,and is suitable for popularization and application in the landscape of the Yangtze River basin and north of the Yangtze River.
基金supported by the National Key R&D Program of China(2020YFC2008100)the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2017-12M-3002)+2 种基金the Zhejiang Province Science and Technology Department Key R&D Program(2021C03097 and 2022C03063)the Binjiang Institute of Zhejiang University(ZY202205SMKY001)the Natural Science Foundation of Zhejiang Province(LQ22H020008)。
基金supported by the Zhejiang Province Science and Technology Department Key R&D Program(No.2021C03097),China.
文摘Considering the surgical risk stratification for patients with severe calcific aortic stenosis(AS),transcatheter aortic valve replacement(TAVR)is a reliable alternative to surgical aortic valve replacement(SAVR)(Fan et al.,2020,2021;Lee et al.,2021).Despite the favorable clinical benefits of TAVR,stroke remains a dreaded perioperative complication(Auffret et al.,2016;Kapadia et al.,2016;Kleiman et al.,2016;Huded et al.,2019).Ischemic overt stroke,identified in 1.4%to 4.3%of patients in TAVR clinical practice,has been associated with prolonged disability and increased mortality(Auffret et al.,2016;Kapadia et al.,2016;Levi et al.,2022).The prevalence of hyperintensity cerebral ischemic lesions detected by diffusion-weighted magnetic resonance imaging(DW-MRI)was reported to be about 80%,which is associated with impaired neurocognitive function and vascular dementia(Vermeer et al.,2003;Barber et al.,2008;Kahlert et al.,2010).
基金supported by the National Key R&D Program of China(Nos.2019YFA0110400 and 2016YFC13010204)the National Natural Science Foundation of China(Nos.81870292,81570233,81770252)+1 种基金the Key Social Development Program of Major Science and Technology Projects in Zhejiang Province(No.2015C03028)the Fundamental Research Funds for the Central Universities(No.2019XZZX005-4-03),China。
文摘Aortic stenosis(AS)is a progressive heart valve disease occurring predominantly in older patients.According to a survey in a western country,the prevalence of AS is nearly 6.4%in patients over 75 years old(Carabello and Paulus,2009).Transcatheter aortic valve replacement(TAVR)is an alternative method for AS patients.Previous studies have described how up to 66%of TAVR patients have concomitant baseline kidney dysfunction(Ferro et al.,2015;Gargiulo et al.,2015).The majority of patients can benefit from the TAVR procedure with the recovery of kidney function.
基金This work was supported by the Zhejiang Province Science and Technology Department Key R&D Program(Nos.2021C03097 and 2018C03084),China.
文摘Transcatheter aortic valve replacement(TAVR)has emerged as a viable treatment option for patients with severe aortic stenosis regardless of its surgical risk stratification(Otto et al.,2021).Aortic angulation is usually measured as the angle between the horizon-tai and the aortic annulus planes based on preprocedural multidetector computed tomography(MDCT)(Al-Lamee et al.,2011).Extremely horizontal aorta,defined as an aortic angulation greater than 70°.
