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Exploring the Mechanism of Action of Glyasperin A in Intervening Menopause Based on Network Pharmacology and Molecular Docking Technology
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作者 Na LI Shunhuan CHEN +3 位作者 xiang pu Yihui CHAI Yuqi YANG Lailai LI 《Medicinal Plant》 2024年第3期4-8,共5页
[Objectives]To investigate the mechanism of action of glyasperin A(GAA)in intervening menopause using network pharmacology and molecular docking technology.[Methods]All target names of the active ingredients were scre... [Objectives]To investigate the mechanism of action of glyasperin A(GAA)in intervening menopause using network pharmacology and molecular docking technology.[Methods]All target names of the active ingredients were screened using TCMSP,3D model molecules converted into SMILES online tool,Swiss target prediction and literature search.The relevant target genes corresponding to menopause were identified using the Genecards database.Venn 2.1.0 was then used to generate the corresponding Venn diagram.Finally,the protein-protein interaction(PPI)network was constructed using Cytoscape 3.9.1 software.The core targets that were screened underwent enrichment and analysis using the Gene Ontology(GO)biological process and KEGG pathways with the assistance of the DAVID database and bioinformatics.The molecular docking was then verified using AutoDock and Pymol software on the core targets.[Results]This study screened 100 target genes of active ingredients.In the PPI network,ESR1 and AKT1 were found to have a higher degree.The GO and KEGG enrichment analyses revealed that the biological processes primarily involved platelet activation,regulation of circadian rhythms,and regulation of mRNA stability.The signalling pathways included hepatitis B,cytotoxicity,and gastric cancer.The molecular docking results indicated that the key active ingredients and proteins bound well,as evidenced by their small binding energies.[Conclusions]Using a systematic network pharmacology approach,this study predicts the basic pharmacological effects and potential mechanisms of GAA in intervening menopause,which provides a foundation for further research on its pharmacological mechanisms. 展开更多
关键词 NETWORK PHARMACOLOGY MOLECULAR DOCKING MENOPAUSE Glyasperin A
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Study on the Mechanism of Action of Glyasperin A in the Treatment of Atherosclerosis Based on Network Pharmacology and Molecular Docking
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作者 Na LI xiang pu +2 位作者 Yihui CHAI Yuqi YANG Lailai LI 《Agricultural Biotechnology》 2024年第2期53-57,共5页
[Objectives] This study was conducted to investigate the mechanism of action of glyasperin A in the treatment of atherosclerosis using a network pharmacology approach. [Methods] Targets related to atherosclerosis were... [Objectives] This study was conducted to investigate the mechanism of action of glyasperin A in the treatment of atherosclerosis using a network pharmacology approach. [Methods] Targets related to atherosclerosis were searched in GeneCards database. An active ingredient-disease-target network was constructed by Cytoscape 3.7.1. A target protein interaction network was constructed by String database. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the DAVID database. [Results] Glyasperin A acted on 36 atherosclerosis-related targets, and the biofunctional and pathway enrichment analyses showed that it was mainly involved in response to xenobiotic stimulus, drug transport across blood-brain barrier, lipid oxidation, barrier, and lipid oxidation, etc. The results showed that glyasperin A acted on 36 atherosclerosis-related targets. The biofunctional and pathway enrichment analyses showed that it was mainly involved in response to xenobiotic stimulus, drug transport across blood-brain barrier, lipid oxidation, positive regulation of protein localization to nucleus, and hepoxilin biosynthetic process, and it played an anti-fatigue role through signal pathways such as serotonergic synapse, efferocytosis, arachidonic acid metabolism, chemical carcinogenesis-receptor activation and platelet activation. [Conclusions] Glyasperin A has multi-target and multi-pathway effects in the treatment of atherosclerosis. This study provides reference for further research on glyasperin A in the treatment of atherosclerosis. 展开更多
关键词 Glyasperin A ATHEROSCLEROSIS Network pharmacology Mechanism of action
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Therapeutic Effects and Potential Mechanisms of Glyasperin A against Myocardial Ischemia Based on Network Pharmacology and Molecular Docking
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作者 Na LI Shunhuan CHEN +3 位作者 xiang pu Yihui CHAI Yuqi YANG Lailai LI 《Asian Agricultural Research》 2024年第5期25-28,共4页
[Objectives]To explore the therapeutic effects and potential mechanisms of Glyasperin A(GAA)on myocardial ischemia(MI)based on network pharmacology and molecular docking.[Methods]The molecular structure of GAA was dow... [Objectives]To explore the therapeutic effects and potential mechanisms of Glyasperin A(GAA)on myocardial ischemia(MI)based on network pharmacology and molecular docking.[Methods]The molecular structure of GAA was downloaded from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and all targets of GAA were predicted by converting 3D model molecules into SMILES online tool and Swiss target prediction.Genecards database and DisGeNET database were used to find the targets related to MI,and then Venny 2.1.0 was used to generate the corresponding Wayne diagram,and then Cytoscape 3.9.1 software was used to construct the protein-protein interaction(PPI)network.With the help of DAVID database and Microbiology,the selected core targets were enriched and analyzed by gene ontology(GO),biological process(BP),and Kyoto Encyclopedia of Genes and Genomes(KEGG),and then the molecular docking between GAA and core targets was verified by AutoDock and Pymol software.[Results]A total of 1883 MI targets were screened,and in the protein-protein interaction network,AKT1,PTGS2,PPARG,ESR1,GSK3B were the proteins with higher values.Gene ontology and KEEG enrichment analysis showed that the biological processes involved mainly included inflammatory response,negative regulation of gene expression,and response to exogenous stimuli.Signaling pathways mainly include IL-17 signaling pathway,HIF-1 signaling pathway,and so on.The results of molecular docking showed that the binding energy of GAA and core protein was less than-5 Kcal/mol in four groups.These indicated that GAA with good binding had a certain therapeutic effect on myocardial ischemia.[Conclusions]Based on the systematic network pharmacology method,this study predicts the basic pharmacological effects and potential mechanisms of GAA in the treatment of MI,and reveals that GAA may treat MI through multiple targets and signaling pathways.It is expected to provide a basis for further study of its pharmacological mechanisms. 展开更多
关键词 Network pharmacology Molecular docking Glyasperin A(GAA) Myocardial ischemia(MI)
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Therapeutic Effect and Mechanism of Jipei Dilong Ointment on Acute Soft Tissue Injury in Rats
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作者 Yihui CHAI Haotian WANG +6 位作者 Shiyun YE Lailai LI Baoying HUA Jinghua RUAN xiang pu Liyan ZHANG Sibu MA 《Agricultural Biotechnology》 CAS 2023年第5期35-42,共8页
[Objectives]This study was conducted to observe the therapeutic effect of Jipei Dilong Ointment on rats with acute soft tissue injury caused by heavy objects and to explore its action mechanism.[Methods]Thirty six rat... [Objectives]This study was conducted to observe the therapeutic effect of Jipei Dilong Ointment on rats with acute soft tissue injury caused by heavy objects and to explore its action mechanism.[Methods]Thirty six rats were randomly divided into six groups(control group,model group,high-dose Jipei Dilong Ointment group(JP-H),medium-dose Jipei Dilong Ointment group(JP-M),low-dose Jipei Dilong Ointment group(JP-L)and diclofenac group).Except for the Control group,other groups were subjected to modeling of acute soft tissue injury by the weight impact method.All administration was performed once a day for nine consecutive days.The local appearance score and activity disorder score were determined after soft tissue injury in rats.HE staining was used to detect the pathological changes of injured soft tissues in rats.RT-PCR was used to detect the relative mRNA expressions of Bax,Bcl-2,MMP-9 and TIMP-1 in injured soft tissues of rats.Western Blot was used to detect the protein expressions of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB p65 in injured soft tissues of rats.Results were statistically analyzed.[Results]Compared with the model group,Jipei Dilong Ointment could significantly improve the appearance symptoms such as swelling and ecchymosis in the injured area and the movement function of the affected limb(P<0.05).It could also improve the infiltration of inflammatory cells and widening of the intermuscular space caused by injury.Among them,the JP-H group and the diclofenac group had more significant curative effects.After 9 d of administration,each administration group could significantly up-regulate the ratio of Bcl-2/Bax mRNA expression level(P<0.05 or P<0.01),and the ratio of MMP-9/TIMP-1 mRNA expression level showed a downward trend(P>0.05).The expression level of NF-κB p65 protein in each administration group was significantly decreased(P<0.01).The protein expression levels of TLR4 and MyD88 and the ratio of MMP-9/TIMP-1 protein expression level in each administration group decreased to varying degrees.Among them,the JP-H group and diclofenac group significantly decreased(P<0.05).[Conclusions]Jipei Dilong Ointment has the functions of relieving pain,swelling and inflammation.It could improve the local appearance,functional activity and tissue morphology of affected limbs in rats,and has a therapeutic effect on acute soft tissue injury in rats.Its mechanism of action might be related to the inhibition of TLR4/MyD88/NF-κB p65 signaling pathway and the regulation of Bcl-2/Bax and MMP-9/TIMP-1 balance. 展开更多
关键词 Jipei Dilong Ointment Acute soft tissue injury NF-κB p65 TLR4 MYD88
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Study on the Mechanism of Pseudostellariae Radix in Regulating Angiogenesis Based on Network Pharmacology and a Dual-screening System
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作者 Na LI Yihui CHAI +4 位作者 Yuqi YANG xiang pu Guo FENG Sibu MA Lailai LI 《Agricultural Biotechnology》 CAS 2023年第5期98-103,113,共7页
[Objectives]This study was conducted to clarify the action mechanism of Pseudostellariae Radix in regulating angiogenesis by using network pharmacology and a dual-screening system,and to provide a basis for its clinic... [Objectives]This study was conducted to clarify the action mechanism of Pseudostellariae Radix in regulating angiogenesis by using network pharmacology and a dual-screening system,and to provide a basis for its clinical treatment of cardiovascular diseases.[Methods]The TCMSP database was used for preliminary screening to obtain the active compounds of Pseudostellariae Radix and the protein targets of its action.GeneCards and OMIM databases were used to search for targets related to angiogenesis.Cytoscape 3.9.1 was used to construct a drug-target network and protein interaction network of Pseudostellariae Radix in angiogenesis.The GO enrichment analysis and KEGG pathway analysis of the targets of Pseudostellariae Radix in angiogenesis were carried out on Metascape platform.The effects of the screened active compounds were verified using a dual-screening system.[Results]Six active components of Pseudostellariae Radix,luteolin,acetin,beta-sitosterol,linarin,schottenol and 1-monolinolein,were screened by TCMSP database;and the six active components were predicted with 78 common target proteins related to angiogenesis,of which 19 were core targets.Pseudostellariae Radix mainly intervened in angiogenesis through domain specific binding,ubiquitin-like protein ligase binding,kinase binding and other molecular functions to regulate biological processes such as membrane microdomain,plasma membrane raft and caveola.The results of KEGG enrichment indicated that pathways in cancer,lipid and atherosclerosis,hepatitis B,apoptosis,toxoplasmosis and other key pathways might be the mechanism for the intervention of angiogenesis.The results of the dual-screening system showed that luteolin,acacetin,beta-sitosterol and linarin protected HUVECs and promoted zebrafish angiogenesis.[Conclusions]This study preliminarily demonstrated that luteolin,acacetin,beta-sitosterol and linarin could intervene in angiogenesis through multiple targets and multiple pathways,providing ideas and a scientific basis for the treatment of cardiovascular diseases. 展开更多
关键词 Pseudostellariae Radix ANGIOGENESIS Network pharmacology Dual-screening system
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Protective Effect and Molecular Mechanism of Shui People's Classic Prescription Jipei Dilong Ointment on Osteoarthritis in Rats
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作者 Lailai LI Baoying HUA +6 位作者 Shiyun YE Yihui CHAI Haotian WANG Jinghua RUAN xiang pu Liyan ZHANG Sibu MA 《Medicinal Plant》 CAS 2023年第5期57-64,共8页
[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control gr... [Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1. 展开更多
关键词 Jipei Dilong Ointment Osteoarthritis(OA) Traditional Chinese medicine application
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Study on the Molecular Mechanism of Danggui Buxue Decoction in Intervention of Perimenopausal Syndrome Based on Network Pharmacology and Molecular Docking
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作者 Na LI Shunhuan CHEN +3 位作者 xiang pu Yihui CHAI Yuqi YANG Lailai LI 《Agricultural Biotechnology》 2023年第6期1-5,共5页
[Objectives]This study was conducted to explore the intervention mechanism of Danggui Buxue Decoction in perimenopausal syndrome based on network pharmacology and molecular docking.[Methods]The chemical components and... [Objectives]This study was conducted to explore the intervention mechanism of Danggui Buxue Decoction in perimenopausal syndrome based on network pharmacology and molecular docking.[Methods]The chemical components and targets of Danggui Buxue Decoction were acquired through the TCMSP database,and the main targets of perimenopausal syndrome were obtained through the GeneCards database.The component targets and disease targets were intersected,and combining with active components and Chinese herbs in the decoction,a traditional Chinese medicine-component-target network was constructed using Cytoscape 3.7.1 software.The STRING platform was employed for protein-protein interaction analysis.The DAVID analysis platform was used to conduct target GO and KEGG enrichment analysis,so as to predict the action mechanism Danggui Buxue Decoction.Finally,an active component-disease target-signal pathway network diagram was constructed.[Results]Twenty two components in Danggui Buxue Decoction related to perimenopausal syndrome and 120 corresponding targets were obtained,including active components such as 1,7-dihydroxy-3,9-dimquercetin and kaempferol,and key targets such as TNF,ESR1 and PPARG.The results of GO analysis and KEEG analysis indicated that Danggui Buxue Decoction might regulate the transcription of RNA polymerase II promoter,DNA templating,gene expression,signal transduction,hypoxia response and other biological processes by regulating multiple signal pathways such as chemical carcinogenesis-receptor activation,cancer pathways,lipid and atherosclerosis,tryptophan metabolism,malaria,steroid hormone biosynthesis and chemical carcinogenesis-DNA adduct.[Conclusions]Danggui Buxue Decoction intervenes in perimenopausal syndrome through multiple components,targets and pathways,providing a basis for elucidating the intervention mechanism of Danggui Buxue Decoction and expanding its clinical application. 展开更多
关键词 Danggui Buxue Decoction Perimenopausal syndrome Network pharmacology Molecular docking
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ZNRF2抑制自噬保护氧糖剥夺/复糖复氧致PC12细胞损伤 被引量:3
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作者 顾超 谭晓丹 +3 位作者 向葡 罗映 杨俊卿 王红 《中国药理学通报》 CAS CSCD 北大核心 2021年第6期768-774,共7页
目的观察膜相关E3泛素连接酶ZNRF2对OGD/R致PC12细胞损伤的保护作用,并从自噬初步探究其机制。方法体外培养PC12细胞,将PC12细胞分为正常组、模型组,采用qRT-PCR、Western blot检测细胞中ZNRF2 mRNA和蛋白的表达。为了进一步探讨ZNRF2对... 目的观察膜相关E3泛素连接酶ZNRF2对OGD/R致PC12细胞损伤的保护作用,并从自噬初步探究其机制。方法体外培养PC12细胞,将PC12细胞分为正常组、模型组,采用qRT-PCR、Western blot检测细胞中ZNRF2 mRNA和蛋白的表达。为了进一步探讨ZNRF2对OGD/R致PC12细胞损伤的作用,将细胞分为正常对照组、模型组、ZNRF2过表达慢病毒组、空载病毒组、ZNRF2小干扰RNA组、阴性对照组,采用MTT实验检测细胞活力、流式细胞术检测细胞凋亡率、Western blot检测各组细胞自噬相关蛋白(LC3、p62、Beclin-1)表达改变。结果与正常组相比,模型组中细胞ZNRF2 mRNA和蛋白表达明显降低,细胞活力明显降低,细胞凋亡率明显升高,LC3II、Beclin-1蛋白水平升高,p62蛋白表达明显下降;与模型组相比,LV-ZNRF2组细胞活力增加,细胞凋亡率和自噬水平减少,siR-ZNRF2组细胞活力明显下降,细胞凋亡率和自噬水平明显上升。结论ZNRF2对OGD/R致PC12细胞损伤有明显保护作用,其机制可能与抑制自噬相关。 展开更多
关键词 氧糖剥夺/复糖复氧 PC12细胞 ZNRF2 自噬 MTOR 凋亡
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下调lncRNA MALAT1表达保护PC12细胞免于氧糖剥夺/复糖复氧所致的损伤 被引量:1
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作者 谭晓丹 顾超 +3 位作者 彭哲 向葡 涂钰均 杨俊卿 《中国病理生理杂志》 CAS CSCD 北大核心 2021年第6期998-1003,共6页
目的:探讨长链非编码RNA(lncRNA)肺腺癌转移相关转录本1(MALAT1)对氧糖剥夺/复糖复氧(OGD/R)诱导PC12细胞损伤的作用。方法:体外培养PC12细胞,分为4组(n=3):正常(normal)组、模型(OGD/R)组、OGD/R+si-NC组(si-NC组)和OGD/R+MALAT1 siRNA... 目的:探讨长链非编码RNA(lncRNA)肺腺癌转移相关转录本1(MALAT1)对氧糖剥夺/复糖复氧(OGD/R)诱导PC12细胞损伤的作用。方法:体外培养PC12细胞,分为4组(n=3):正常(normal)组、模型(OGD/R)组、OGD/R+si-NC组(si-NC组)和OGD/R+MALAT1 siRNA组(siRNA组)。RT-qPCR检测MALAT1的表达,MTT法检测PC12细胞活力,流式细胞术检测PC12细胞凋亡率,Western blot分析环加氧酶2(COX-2)、Bax及Bcl-2蛋白的表达,ELISA试剂盒检测炎症因子的含量。结果:同normal组相比,OGD/R组MALAT1表达升高(P<0.01),细胞活力显著降低(P<0.01),细胞凋亡率显著升高(P<0.01),炎症因子前列腺素E2(PGE2)和肿瘤坏死因子α(TNF-α)的表达显著增加(P<0.01),COX-2和Bax表达显著上调,Bcl-2和IL-10含量显著降低(P<0.01)。同si-NC组相比,siRNA干扰MALAT1的表达后,PC12细胞活力显著提高(P<0.01),细胞凋亡率显著降低(P<0.05),炎症因子PGE2和TNF-α表达显著减少(P<0.05或P<0.01),IL-10表达明显增加(P<0.05),COX-2和Bax表达显著下调(P<0.05),Bcl-2明显上调(P<0.05)。结论:下调MALAT1表达可减轻OGD/R诱导的PC12细胞损伤,其机制可能涉及减轻炎症反应和抑制细胞凋亡。 展开更多
关键词 氧糖剥夺 长链非编码RNA 肺腺癌转移相关转录本1 细胞凋亡 环加氧酶2 炎症
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“双一流”背景下高等农业院校有机化学基础课程教学新模式的构建及实践 被引量:9
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作者 蒲祥 王晗光 +8 位作者 邹平 王广途 乐贵洲 刘宽 程琍 冯鞠花 张慧贤 黄乾明 饶含兵 《大学化学》 CAS 2021年第1期29-38,共10页
四川农业大学作物学入选一流学科建设,有机化学作为农林专业创新型人才培养的战略支撑,如何把握机遇,找准定位,深化有机化学基础课教学改革,为农林类学科培养基础扎实、创新意识强的优质生源是我们长期面临的挑战。以一流学科建设为导向... 四川农业大学作物学入选一流学科建设,有机化学作为农林专业创新型人才培养的战略支撑,如何把握机遇,找准定位,深化有机化学基础课教学改革,为农林类学科培养基础扎实、创新意识强的优质生源是我们长期面临的挑战。以一流学科建设为导向,通过调研确立双一流建设期有机化学基础课实施性教学方案,构建有机化学立体化教学资源库,实践“兴趣培养–思维开发–自主协作学习”课堂教学模式与“基础技能–综合应用–探索创新”梯度式实践教学模式,在农学、林学、动物医学等共35个专业推广,提升创新型人才培养质量,有效支撑我校一流学科建设,为同类农林高校有机化学基础课教学提供参考。 展开更多
关键词 双一流 农林院校 有机化学 教学模式 构建与实践
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“新农科”背景下四川农业大学一流化学专业建设 被引量:4
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作者 蒲祥 李云春 +3 位作者 王广途 饶含兵 黄乾明 吴贺君 《大学化学》 CAS 2022年第8期10-16,共7页
自教育部启动“双万计划”以来,四川农业大学积极响应号召,对化学生物学与应用化学本科专业进行升级改造。在新农科背景下,紧密围绕化学专业定位,依托生物资源化学、生物与医药“本硕博”培养体系以及“化学”和“生物与生物化学”双学... 自教育部启动“双万计划”以来,四川农业大学积极响应号召,对化学生物学与应用化学本科专业进行升级改造。在新农科背景下,紧密围绕化学专业定位,依托生物资源化学、生物与医药“本硕博”培养体系以及“化学”和“生物与生物化学”双学科优势,从师资队伍与教学团队建设、特色课程体系与一流课程建设、实验平台与科研团队建设、学生培养体系建设等方面进行了积极探索。我校化学生物学已获批四川省本科一流专业建设点,“化学”学科于2022年跻身ESI全球前1%。为同类农业院校本科化学专业建设提供参考。 展开更多
关键词 农业院校 新农科 一流专业 化学生物学 应用化学
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泰利霉素关键中间体3-OH-6-O-甲基红霉素的合成研究 被引量:1
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作者 王金龙 向普 +1 位作者 王宗利 侯仲轲 《精细化工中间体》 CAS 2018年第2期50-54,共5页
对泰利霉素关键中间体3-羟基-6-O-甲基红霉素(4)的合成进行了研究,以克拉霉素前体2′,4″-O-二(三甲基硅基)-6-O-甲基红霉素A9-O-(1-乙氧基异丙叉基)肟(1)为原料,通过对一步法工艺优化得到产品4,通过使用缓冲体系和两边同时滴加盐酸和Na... 对泰利霉素关键中间体3-羟基-6-O-甲基红霉素(4)的合成进行了研究,以克拉霉素前体2′,4″-O-二(三甲基硅基)-6-O-甲基红霉素A9-O-(1-乙氧基异丙叉基)肟(1)为原料,通过对一步法工艺优化得到产品4,通过使用缓冲体系和两边同时滴加盐酸和NaNO_2的方式来控制脱肟过程的pH,该方法显著提高了产品纯度,缩短了反应时间,可有效抑制半缩酮副产物的生成,制得高纯度目标产物4,可与克拉霉素联产,大大简化了合成工艺,具有工业化前景。 展开更多
关键词 2′ 4″-O-二(三甲基硅基)-6-O-甲基红霉素A9-O-(1-乙氧基异丙叉基)肟 3-OH-6-O-甲基红霉素 一步法合成 克拉霉素
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塞利尼索联合维奈克拉、阿扎胞苷方案诱导治疗复发难治急性髓系白血病的疗效及安全性观察
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作者 刘丽娜 崔玉山 +5 位作者 刘玉章 王耀美 向谱 梁利杰 李以冉 房佰俊 《中华血液学杂志》 CAS CSCD 北大核心 2024年第8期772-775,共4页
为探讨塞利尼索联合维奈克拉(VEN)、阿扎胞苷(AZA)在复发难治急性髓系白血病(R/R AML)患者中的疗效及安全性,纳入2022年5月至2023年5月在郑州大学附属肿瘤医院接受塞利尼索联合VEN、AZA方案的12例R/R AML患者,对其临床资料进行回顾性分... 为探讨塞利尼索联合维奈克拉(VEN)、阿扎胞苷(AZA)在复发难治急性髓系白血病(R/R AML)患者中的疗效及安全性,纳入2022年5月至2023年5月在郑州大学附属肿瘤医院接受塞利尼索联合VEN、AZA方案的12例R/R AML患者,对其临床资料进行回顾性分析。12例患者中,完全缓解(CR)5例(41.7%),CR伴血液学不完全恢复1例(8.3%),部分缓解5例(41.7%)。达CR中位时间28(16~59)d。中位无病生存期为61(15~300)d。该方案的主要不良反应为血液学不良反应,无化疗相关死亡。塞利尼索联合VEN、AZA方案是治疗R/R AML的有效治疗手段。 展开更多
关键词 疗效及安全性 阿扎胞苷 诱导治疗 复发难治急性髓系白血病 不良反应 部分缓解 AML 无病生存期
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miR-204-5p在氧糖剥夺/复糖复氧致SH-SY5Y细胞损伤中的作用研究 被引量:1
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作者 涂钰均 向葡 +3 位作者 郭文佳 顾超 谭晓丹 杨俊卿 《中国药学杂志》 CAS CSCD 北大核心 2022年第2期116-123,共8页
目的观察miR-204-5p在氧糖剥夺/复糖复氧(oxygen-glucose deprivation/reoxygenation, OGD/R)致人神经母细胞瘤细胞(SH-SY5Y)损伤中的作用并从炎症/凋亡途径探讨其机制。方法 SH-SY5Y细胞分为control组、OGD/R组、OGD/R+miR-204-5p mimi... 目的观察miR-204-5p在氧糖剥夺/复糖复氧(oxygen-glucose deprivation/reoxygenation, OGD/R)致人神经母细胞瘤细胞(SH-SY5Y)损伤中的作用并从炎症/凋亡途径探讨其机制。方法 SH-SY5Y细胞分为control组、OGD/R组、OGD/R+miR-204-5p mimic组、OGD/R+miR-204-5p inhibitor组、OGD/R+miR-204-5p mimic negative control组、OGD/R+miR-204-5p inhibitor negative control组。采用MTT法测定细胞增殖、流式细胞术、TUNEL染色法检测细胞凋亡、酶联免疫法检测炎症因子(IL-10、IL-1β、TNF-α、PGE2)含量、qRT-PCR检测miR-204-5p的表达、western blot检测炎症及凋亡相关蛋白(COX-2、Bcl-2、Bax)的表达。结果与对照组相比,OGD/R组细胞miR-204-5p表达显著降低,IL-1β、TNF-α、PGE2含量增多,IL-10含量减少,COX-2、Bax蛋白水平上升,Bcl-2蛋白水平下降,细胞损伤明显加重;与OGD/R组比较,miR-204-5p mimic下调COX-2、Bax蛋白表达,上调Bcl-2蛋白表达,IL-10含量增加,IL-1β、TNF-α、PGE2含量减少,细胞活力明显增加、凋亡率显著降低,细胞损伤减轻。结论 miR-204-5p对OGD/R致SH-SY5Y细胞损伤具有明显保护作用,其机制可能与减轻细胞炎症和凋亡有关。 展开更多
关键词 氧糖剥夺/复糖复氧 人神经母细胞瘤细胞 miR-204-5p 炎症 细胞凋亡
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多发性骨髓瘤患者血浆微小RNA-17-5p的表达及其对肿瘤发生发展的作用 被引量:1
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作者 向谱 刘丽娜 +2 位作者 刘玉章 崔玉山 房佰俊 《中华医学杂志》 CAS CSCD 北大核心 2022年第30期2357-2362,共6页
目的探讨多发性骨髓瘤(MM)患者血浆微小RNA(miR)-17-5p的表达及其对肿瘤发生发展的作用。方法纳入2013年4月至2018年4月在河南省肿瘤医院血液科就诊的意义未明单克隆丙种球蛋白血症(MGUS)患者、MM患者及20名健康志愿者,采用逆转录聚合... 目的探讨多发性骨髓瘤(MM)患者血浆微小RNA(miR)-17-5p的表达及其对肿瘤发生发展的作用。方法纳入2013年4月至2018年4月在河南省肿瘤医院血液科就诊的意义未明单克隆丙种球蛋白血症(MGUS)患者、MM患者及20名健康志愿者,采用逆转录聚合酶链反应(qRT-PCR)法检测血浆循环miR-17-5p和骨髓单个核细胞中miR-17-5p的表达量。其中MM组患者分为初诊未治MM(NDMM)组、完全缓解MM(CRMM)组以及复发难治MM(RRMM)组。分析各组miR-17-5p的表达差异;用相关性分析评估NDMM患者的血浆miR-17-5p与血清M蛋白和骨髓浆细胞比例之间的关系;用受试者工作特征(ROC)曲线评估血浆miR-17-5p作为MM诊断相关的分子标志物的可能性;过表达或敲低miR-17-5p的表达后,细胞计数试剂盒(CCK-8)法检测miR-17-5P对MM细胞系增殖的影响,裸鼠皮下成瘤实验体内检测miR-17-5p对MM细胞增殖的影响。结果纳入MGUS患者10例,其中男6例、女4例。MM患者92例,其中男54例、女38例;MM患者中,NDMM组22例、CRMM组11例、RRMM组59例。NDMM组和RRMM组骨髓单个核细胞中miR-17-5p的表达量高于健康对照组[1.37(0.47,4.87)、2.68(1.02,5.02)比1.00(1.00,1.00),均P<0.05],且血浆miR-17-5p的表达水平也高于健康对照组[1.85(0.92,3.51)、2.79(1.22,5.04)比1.00(1.00,1.00),均P<0.05];miR-17-5p在KMS-11、RPMI-8226、H929、MM-1R、U266B1等MM细胞系中的表达量均高于健康对照组的骨髓单个核细胞(3.96±0.68、1.58±0.32、3.51±0.55、5.08±0.76、3.22±0.75比1.00±0,均P<0.05);血浆miR-17-5p表达与血清M蛋白和骨髓浆细胞比例均呈正相关(r=0.50,P<0.05;r=0.60,P<0.01);ROC曲线显示血浆miR-17-5p作为诊断MM的相关分子标志物的特异度为0.591,灵敏度为0.900(ROC曲线下面积=0.74,cut-off值为0.491);CCK-8结果提示miR-17-5p过表达使RPMI-8226和NCI-H929等MM细胞系72 h的增殖较对照组增高(1.37±0.11比1.07±0.09,2.14±0.09比1.82±0.11,均P<0.05),miR-17-5p低表达使NCI-H929和MM-1R等MM细胞系72 h的增殖较对照组降低(1.38±0.09比1.83±0.11,1.45±0.10比1.73±0.09,均P<0.05)。裸鼠皮下成瘤实验提示,miR-17-5p过表达组肿瘤体积大于对照组[(1865±181)比(1389±227)mm^(3),P<0.05],miR-17-5p低表达组肿瘤体积小于对照组[(1006±171)比(1389±227)mm^(3),P<0.05]。结论miR-17-5p可能作为MM疾病发展状态相关的血浆分子标志物,在MM细胞系中起癌基因作用。 展开更多
关键词 多发性骨髓瘤 微小RNA-17-5p 血浆分子标志物 癌基因 横断面研究
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Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate 被引量:1
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作者 xiang pu Jia Li +7 位作者 Ziang Guo Minji Wang Ming Lei Shengnan Yang Jun Yang Hanguang Wang Li Zhang Qianming Huang 《Synthetic and Systems Biotechnology》 SCIE 2022年第2期824-836,共13页
Flavonoid metabolism in Camptotheca acuminate remained an untapped area for years.A tandem MS approach was used and focused on the mining and characterizing of flavonoids in mature C.acuminate.Fifteen new flavonoids a... Flavonoid metabolism in Camptotheca acuminate remained an untapped area for years.A tandem MS approach was used and focused on the mining and characterizing of flavonoids in mature C.acuminate.Fifteen new flavonoids and forty-three known flavonoids,including fifteen flavone analogs,sixteen flavonol analogs,seven flavanone analogs,six chalcone analogs,four xanthone analogs,ten flavane analogs were mined and identified based on their MS/MS fragments.Fifty-three of them were firstly characterized in C.acuminate.Eight biosynthetic precursors for these flavonoids were also identified.We constructed a specific metabolic map for flavonoids according to their relative contents in the flowers,fruits,stems,and leaves of C.acuminate.Furthermore,the most probable genes involved in chalcone biosynthesis,flavonoid hydroxylation,methylation,and glycosylation were further mined and fished in the gene reservoir of C.acuminate according to their conserved domains and co-expression analysis.These findings enable us to acquire a better understanding of versatile flavonoid metabolism in C.acuminate. 展开更多
关键词 Camptotheca acuminate FLAVONOIDS Fragmentation pathway Flavonoid metabolism
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伴复杂核型的原发性浆细胞白血病一例并文献复习
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作者 王娟 梁利杰 +5 位作者 刘玉章 刘丽娜 王耀美 向谱 房佰俊 宋永平 《白血病.淋巴瘤》 CAS 2020年第7期419-422,共4页
目的观察以硼替佐米、地塞米松为基础的三联化疗序贯自体造血干细胞移植后应用来那度胺维持并间断行强化治疗的系列方案治疗原发性浆细胞白血病的效果。方法回顾性分析河南省肿瘤医院2018年4月收治的1例伴复杂核型的原发性浆细胞白血病... 目的观察以硼替佐米、地塞米松为基础的三联化疗序贯自体造血干细胞移植后应用来那度胺维持并间断行强化治疗的系列方案治疗原发性浆细胞白血病的效果。方法回顾性分析河南省肿瘤医院2018年4月收治的1例伴复杂核型的原发性浆细胞白血病患者的临床资料,并对相关文献进行复习。结果该患者接受多个周期以硼替佐米、地塞米松为基础的三联化疗方案序贯自体造血干细胞移植,然后应用来那度胺维持并间断行强化治疗,获得完全缓解,至截稿前无进展生存达18个月。结论以硼替佐米、地塞米松为基础的三联化疗方案序贯自体造血干细胞移植后应用来那度胺维持并间断行强化治疗的系列方案可能会改善原发性浆细胞白血病患者的预后,延长生存时间。 展开更多
关键词 白血病 浆细胞 造血干细胞移植 药物疗法 联合 来那度胺
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