Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who atte...Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who attended Hospital 960 from January 2021 to January 2022 were retrospectively analyzed. Clinical data of the patients were collected, including gender, age, and histologic type. Immunohistochemical indexes such as Th17 cell level and monocyte/ lymphocyte ratio in the peripheral blood of patients were also collected. The prognosis of patients after treatment, as well as peripheral blood Th17 and MLR levels, were observed and analyzed. Results: After follow-up after treatment, in the final 74 patients, the prognosis was good in 32 patients, accounting for 43.24%, and the prognosis was bad in 42 patients, accounting for 56.76%. There were no significant differences between the average age and tumor diameters of the good prognosis and poor prognosis groups (P > 0.05). However, the TNM staging, intervention taken, differentiation degree, presence of distant metastasis, presence of lymph node metastasis, Th17 level, and MLR level are significantly different between the two groups (P < 0.05). Conclusion: Peripheral blood Th17 and MLR have predictive value for the prognosis of colorectal cancer patients, and high levels of peripheral blood Th17 and MLR imply poor prognosis. The detection of peripheral blood Th17 and MLR levels is simple and convenient and can be used as indicators to provide a reference for the prognostic assessment of colorectal cancer patients.展开更多
Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root o...Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.展开更多
To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene express...To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene expression data. Supervised learning approaches predicted that the Ras pathway is active in -70% of lung adenocarcinomas but inactive in most squamous cell carcinomas, pulmonary carcinoids, and small cell lung carcinomas. In contrast, the TGF-β, TNF-α, Src, Myc, E2F3, and β-catenin pathways are inactive in lung adenocarcinomas. We predicted an active Ras, Myc, Src, and/or E2F3 pathway in significant percentages of breast cancer, colorectal carcinoma, and gliomas. Our results also suggest that Ras may be the most prevailing oncogenic pathway. Additionally, many NCI60 cell lines exhibited a gene signature indicative of an active Ras, Myc, and/or Src, but not E2F3, β-catenin, TNF-α, or TGF-β pathway. To our knowledge, this is the first comprehensive survey of cancer pathway activities in nine major tumor types and the most widely used NCI60 cell lines. The "gene expression pathway signatures" we have defined could facilitate the understanding of molecular mechanisms in cancer development and provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screening.展开更多
文摘Objective: To investigate the value of peripheral blood helper T cell 17 cell level and monocyte/lymphocyte ratio to predict the prognosis of colorectal cancer patients. Methods: 74 colorectal cancer patients who attended Hospital 960 from January 2021 to January 2022 were retrospectively analyzed. Clinical data of the patients were collected, including gender, age, and histologic type. Immunohistochemical indexes such as Th17 cell level and monocyte/ lymphocyte ratio in the peripheral blood of patients were also collected. The prognosis of patients after treatment, as well as peripheral blood Th17 and MLR levels, were observed and analyzed. Results: After follow-up after treatment, in the final 74 patients, the prognosis was good in 32 patients, accounting for 43.24%, and the prognosis was bad in 42 patients, accounting for 56.76%. There were no significant differences between the average age and tumor diameters of the good prognosis and poor prognosis groups (P > 0.05). However, the TNM staging, intervention taken, differentiation degree, presence of distant metastasis, presence of lymph node metastasis, Th17 level, and MLR level are significantly different between the two groups (P < 0.05). Conclusion: Peripheral blood Th17 and MLR have predictive value for the prognosis of colorectal cancer patients, and high levels of peripheral blood Th17 and MLR imply poor prognosis. The detection of peripheral blood Th17 and MLR levels is simple and convenient and can be used as indicators to provide a reference for the prognostic assessment of colorectal cancer patients.
基金This research was based on the‘Functional food research and development of Tetrastigma hemsleyanum’funded by the Shanghai Zhengyue Enterprise Management Co.,Ltd.and the Science and Technology Department of Zhejiang Province(No.2018C02045),China.
文摘Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.
文摘To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene expression data. Supervised learning approaches predicted that the Ras pathway is active in -70% of lung adenocarcinomas but inactive in most squamous cell carcinomas, pulmonary carcinoids, and small cell lung carcinomas. In contrast, the TGF-β, TNF-α, Src, Myc, E2F3, and β-catenin pathways are inactive in lung adenocarcinomas. We predicted an active Ras, Myc, Src, and/or E2F3 pathway in significant percentages of breast cancer, colorectal carcinoma, and gliomas. Our results also suggest that Ras may be the most prevailing oncogenic pathway. Additionally, many NCI60 cell lines exhibited a gene signature indicative of an active Ras, Myc, and/or Src, but not E2F3, β-catenin, TNF-α, or TGF-β pathway. To our knowledge, this is the first comprehensive survey of cancer pathway activities in nine major tumor types and the most widely used NCI60 cell lines. The "gene expression pathway signatures" we have defined could facilitate the understanding of molecular mechanisms in cancer development and provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screening.