AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 ...AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 classes in 4 primary schools in Foshan participated in this study.The whole randomization method was adopted to include classes as a group according to 1:1 randomized control.Classrooms in the control group were illuminated by usual light,and classrooms in the intervention group were illuminated by artificial natural light.All students received uncorrected visual acuity and best-corrected visual acuity measurement,non-cycloplegic autorefraction,ocular biometric examination,slit lamp and strabismus examination.Three-year follow-up,the students underwent same procedures.Myopia was defined as spherical equivalent refraction≤-0.50 D and uncorrected visual acuity<20/20.RESULTS:There were 894 students in the control group and 946 students in the intervention group with a mean±SD age of 7.50±0.53y.The three-year cumulative incidence rate of myopia was 26.4%(207 incident cases among 784 eligible participants at baseline)in the control group and 21.2%(164 incident cases among 774 eligible participants at baseline)in the intervention group[difference of 5.2%(95%CI,3.7%to 10.1%);P=0.035].There was also a significant difference in the three-year change in spherical equivalent refraction for the control group(-0.81 D)compared with the intervention group[-0.63 D;difference of 0.18 D(95%CI,0.08 to 0.28 D);P<0.001].Elongation of axial length was significantly different between in the control group(0.77 mm)and the intervention group[0.72 mm;difference of 0.05 mm(95%CI,0.01 to 0.09 mm);P=0.003].CONCLUSION:Artificial natural light in the classroom of primary schools can result in reducing incidence rate of myopia during a period of three years.展开更多
Recent evidence suggests that low-intensity extracorporeal shock wave therapy(Li-ESWT)is a promising treatment for chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS);however,its safety in pelvic organs,particul...Recent evidence suggests that low-intensity extracorporeal shock wave therapy(Li-ESWT)is a promising treatment for chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS);however,its safety in pelvic organs,particularly prostate tissues and cells,remains unclear.The current study evaluates the risks of prostate cell damage or oncogenesis following the administration of LiESWT for prostatitis.To this end,a robust in vitro model(Cell Counting Kit-8[CCK-8]assay,clone formation assay,cell scratch assay,lactate dehydrogenase[LDH]release assay,flow cytometry,and immunoblotting assay)was designed to examine the effects of Li-ESWT on cell proliferation,clonogenicity,migration,membrane integrity,and DNA damage.Exome sequencing of Li-ESWTtreated cells was performed to determine the risk of carcinogenesis.Furthermore,an in vivo rat model(n=20)was employed to assess the effects of Li-ESWT on cancer biomarkers(carcinoembryonic antigen[CEA],Ki67,proliferating cell nuclear antigen[PCNA],and gamma-H2A histone family member X,phosphorylation of the H2AX Ser-139[γ-H2AX])in prostate tissue.Based on our findings,Li-ESWT promotes cellular growth and motility without inducing significant cell membrane or DNA damage or alterations.Genetic analyses did not demonstrate an increase in mutations,and no damage to prostate tissue or upregulation of cancer biomarkers was detected in vivo.This comprehensive in vitro and in vivo assessment confirms the safety of Li-ESWT in managing prostate disorders.展开更多
基金Guangdong Basic and Applied Basic Research Foundation(No.2019B1515120011)Medical Research,Foshan Health and Wellness Department(No.20220374).
文摘AIM:To assess the efficacy of artificial natural light in preventing incident myopia in primary school-age children.METHODS:This is a prospective,randomized control,intervention study.A total of 1840 students from 39 classes in 4 primary schools in Foshan participated in this study.The whole randomization method was adopted to include classes as a group according to 1:1 randomized control.Classrooms in the control group were illuminated by usual light,and classrooms in the intervention group were illuminated by artificial natural light.All students received uncorrected visual acuity and best-corrected visual acuity measurement,non-cycloplegic autorefraction,ocular biometric examination,slit lamp and strabismus examination.Three-year follow-up,the students underwent same procedures.Myopia was defined as spherical equivalent refraction≤-0.50 D and uncorrected visual acuity<20/20.RESULTS:There were 894 students in the control group and 946 students in the intervention group with a mean±SD age of 7.50±0.53y.The three-year cumulative incidence rate of myopia was 26.4%(207 incident cases among 784 eligible participants at baseline)in the control group and 21.2%(164 incident cases among 774 eligible participants at baseline)in the intervention group[difference of 5.2%(95%CI,3.7%to 10.1%);P=0.035].There was also a significant difference in the three-year change in spherical equivalent refraction for the control group(-0.81 D)compared with the intervention group[-0.63 D;difference of 0.18 D(95%CI,0.08 to 0.28 D);P<0.001].Elongation of axial length was significantly different between in the control group(0.77 mm)and the intervention group[0.72 mm;difference of 0.05 mm(95%CI,0.01 to 0.09 mm);P=0.003].CONCLUSION:Artificial natural light in the classroom of primary schools can result in reducing incidence rate of myopia during a period of three years.
基金supported by grants from the National Natural Science Foundation of China(No.PR0121015)the National Natural Science Foundation of China(No.82160148)the Natural Science Foundation Project of Gansu Province(No.2106RJZA110).
文摘Recent evidence suggests that low-intensity extracorporeal shock wave therapy(Li-ESWT)is a promising treatment for chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS);however,its safety in pelvic organs,particularly prostate tissues and cells,remains unclear.The current study evaluates the risks of prostate cell damage or oncogenesis following the administration of LiESWT for prostatitis.To this end,a robust in vitro model(Cell Counting Kit-8[CCK-8]assay,clone formation assay,cell scratch assay,lactate dehydrogenase[LDH]release assay,flow cytometry,and immunoblotting assay)was designed to examine the effects of Li-ESWT on cell proliferation,clonogenicity,migration,membrane integrity,and DNA damage.Exome sequencing of Li-ESWTtreated cells was performed to determine the risk of carcinogenesis.Furthermore,an in vivo rat model(n=20)was employed to assess the effects of Li-ESWT on cancer biomarkers(carcinoembryonic antigen[CEA],Ki67,proliferating cell nuclear antigen[PCNA],and gamma-H2A histone family member X,phosphorylation of the H2AX Ser-139[γ-H2AX])in prostate tissue.Based on our findings,Li-ESWT promotes cellular growth and motility without inducing significant cell membrane or DNA damage or alterations.Genetic analyses did not demonstrate an increase in mutations,and no damage to prostate tissue or upregulation of cancer biomarkers was detected in vivo.This comprehensive in vitro and in vivo assessment confirms the safety of Li-ESWT in managing prostate disorders.
