Genetic abnormalities assist in pathological diagnosis and EBVpositive cell density impact survival in Chinese angioimmunoblastic T-cell lymphoma patients

Objective:To explore the application of genetic abnormalities in the diagnosis of angioimmunoblastic T-cell lymphoma(AITL)and the reliable pathological prognostic factors.Methods:This study included 53 AITL cases,which were reviewed for morphological patterns,immunophenotypes,presence of Hodgkin and Reed-Sternberg(HRS)-like cells,and co-occurrence of B cell proliferation.The Epstein-Barr virus(EBV)-positive cells in tissues were counted,and cases were classified into“EBV encoded RNA(EBER)high-density”group if>50/HPF.Targeted exome sequencing was performed.Results:Mutation data can assist AITL diagnosis:1)with considerable HRS-like cells(20 cases):RHOA mutated in 14 cases(IDH2 co-mutated in 3 cases,4 cases with rare RHOA mutation),TET2 was mutated in 5 cases(1 case comutated with DNMT3A),and DNMT3A mutated in 1 case;2)accompanied with B cell lymphoma(7 cases):RHOA mutated in 4 cases(1 case had IDH2 mutation),TET2 mutated in 2 cases and DNMT3A mutated in 1 case;3)mimic peripheral T cell lymphoma,not otherwise specified(5 cases):RHOA mutated in 2 cases(IDH2 co-mutated in 1 case),TET2 mutated in 3 cases,and DNMT3A mutated in 1 case;4)pattern 1(1 case),RHOA and TET2 co-mutated.Besides RHOAG17V(30/35),rare variant included RHOAK18N,RHOAR68H,RHOAC83Y,RHOAD120G and RHOAG17del,IDH2R172 co-mutated with IDH2M397V in one case.There were recurrent mutations of FAT3,PCLO and PIEZO1 and genes of epigenetic remodeling,T-cell activation,APC and PI3K/AKT pathway.EBER high-density independently indicated adverse overall survival and progression-free survival(P=0.046 and P=0.008,KaplanMeier/log-rank).Conclusions:Over half AITL cases might be confused in diagnosis for certain conditions without mutation data.Targeted exome sequencing with a comprehensive panel is crucial to detect both hot-spot and rare mutation variants for RHOA and IDH2 and other recurrent mutated genes in addition to TET2 and DNMT3A.EBER highdensity independently indicated adverse survival.

Study on vibration reduction of two-scale system coupled with dynamic vibration absorber

The dynamic vibration absorber with inerter and grounded stiffness(IGDVA)is used to control a two-scale system subject to a weak periodic perturbation.The vibration suppression effect is remarkable.The amplitude of the main system coupled with absorber is significantly reduced,and the high frequency vibration completely disappears.First,through the slow-fast analysis and stability theory,it is found that the stability of the autonomous system exerts a notable regulating effect on the vibration response of the non-autonomous system.After adding the dynamic vibrator absorber,the center in the autonomous system changes to an asymptotically stable focus,consequently suppressing the vibration in the non-autonomous system.Further research reveals that the parameters of the absorber affect the real parts of the eigenvalues of the autonomous system,thereby regulating the stability of the system.Transitioning from a qualitative standpoint to a quantitative approach,a comparison of the solutions before and after the introduction of the dynamic absorber reveals that,when the grounded stiffness ratio and the mass ratio of the dynamic absorber are not equal,the high-frequency part in the analytical solution disappears.As a result,this leads to a reduction in the amplitude of the trajectory,achieving a vibration reduction effect.

“新农科”背景下林业高校化学类专业“干燥花制作原理与技术”特色课程建设

“新农科”背景下,林业高校对化学类专业人才培养提出了更高要求。“干燥花制作原理与技术”课程根据花卉事业发展需要,着重阐述干燥花的制作技艺和研究开发,并从教材、师资、考核、双创等方面强化课程特色建设,以便更好地提升教学效果,为创新型农林人才的培养打下基础。

Red tetrazolium as an effective inhibitor for the corrosion of cold rolled steel in 7.0 mol·L^(-1) H_2SO_4 solution

The corrosion inhibition of cold rolled steel(CRS) in 7.0 mol·L^(-1) H_2 SO_4 solution by red tetrazolium(RTZ) was carefully investigated using both experimental procedures and theoretical techniques. The results show that RTZ acts as an effective inhibitor for the corrosion of CRS in 7.0 mol·L^(-1) H_2SO_4, and the maximum inhibition efficiency is higher than 95% with a RTZ concentration of 2.0 mmol·L^(-1). The adsorption of RTZ on CRS surface follows Langmuir isotherm. RTZ effectively retards both cathodic and anodic reactions, and acts as a mixed-type inhibitor. EIS exhibits two capacitive loops, and their resistances increase drastically in the presence of RTZ. SEM and AFM confirm that the addition of RTZ could significantly retard the corrosion of CRS surface. A series of characterizations like FTIR, RS, XRD and XPS reveal that the corrosion CRS surface is composed of the corrosion products of iron sulfates, iron oxides and iron hydroxide, as well as inhibitor. Theoretical results of quantum chemical calculation and molecular dynamics(MD) indicate that the adsorption center of RTZ+(organic cationic part of RTZ) mainly relies on its tetrazole ring,and the adsorption of RTZ+on Fe(001) surface is in a nearly flat orientation mode.

比色法测碘酸铜溶度积常数实验的改进

现行教材中以氨水做显色剂,比色法测定碘酸铜溶度积常数,有一定的环境污染。本实验以甘氨酸替代氨水作为显色剂,通过紫外-可见吸收光谱考察甘氨酸与铜离子反应的时间以及甘氨酸的最佳用量,从而获得配制甘氨酸合铜(Ⅱ)溶液的最佳条件,进而测定该条件下配制的各溶液的吸光度,最终获得碘酸铜饱和溶液中铜离子的浓度并计算得到与文献值接近的溶度积常数。该实验可避免实验过程中挥发性腐蚀性气体的释放,且溶液可浓缩回收甘氨酸合铜(Ⅱ),避免了环境污染,是一种绿色实验方法,在人数众多的基础实验教学中具有适用性。

Approximate analytical solution in slow-fast system based on modified multi-scale method

A simple,yet accurate modi?ed multi-scale method(MMSM)for an approximately analytical solution in nonlinear oscillators with two time scales under forced harmonic excitation is proposed.This method depends on the classical multi-scale method(MSM)and the method of variation of parameters.Assuming that the forced excitation is a constant,one could easily obtain the approximate analytical solution of the simpli?ed system based on the traditional MSM.Then,this solution for the oscillator under forced harmonic excitation could be established after replacing the harmonic excitation by the constant excitation.To certify the correctness and precision of the proposed analytical method,the van der Pol system with two scales subject to slowly periodic excitation is investigated;this system presents rich dynamical phenomena such as spiking(SP),spiking-quiescence(SP-QS),and quiescence(QS)responses.The approximate analytical expressions of the three types of responses are given by the MMSM,and it can be found that the precision of the new analytical method is higher than that of the classical MSM and better than that of the harmonic balance method(HBM).The results obtained by the present method are considerably better than those obtained by traditional methods,quantitatively and qualitatively,particularly when the excitation frequency is far less than the natural frequency of the system.

An efficient corrosion inhibitor of cassava starch graft copolymer for aluminum in phosphoric acid

Starch is one of the richest natural polymers with low-cost,non-toxic and biodegradable,but is seldom directly used as corrosion inhibitor due to its poor inhibitive ability and low water solubility.To solve this problem,cassava starch-acryl amide graft copolymer(CS-AAGC)was prepared through grafting acryl amide(AA)with cassava starch(CS),and it was firstly examined as an efficient inhibitor for 1060 aluminum in 1.0 mol·L^(-1)H_(3)PO_(4) media.The adsorption behavior of CS-AAGC and its electrochemical mechanism were investigated by weight loss and electrochemical methods.Additionally,the inhibited aluminum surface was fully characterized by a series of SEM,AFM,contact angle measurements and XPS.Results confirm that CS-AAGC performs better inhibitive ability than CS,AA or CS/AA mixture,and the maximum inhibition efficiency of 1.0 g·L^(-1)CS-AAGC is 90.6%at 20℃.CS-AAGC acts as a mixed-type inhibitor while mainly retards the anodic reaction.EIS has three time constants,and the polarization resistance is significantly increased in the presence of CS-AAGC.The micrograph of inhibited aluminum surface is of hydrophobic nature with low surface roughness and little corrosion degree.

Primary resonance of fractional-order Duffing–van der Pol oscillator by harmonic balance method

The dynamical properties of fractional-order Duffing–van der Pol oscillator are studied, and the amplitude–frequency response equation of primary resonance is obtained by the harmonic balance method. The stability condition for steady-state solution is obtained based on Lyapunov theory. The comparison of the approximate analytical results with the numerical results is fulfilled, and the approximations obtained are in good agreement with the numerical solutions. The bifurcations of primary resonance for system parameters are analyzed. The results show that the harmonic balance method is effective and convenient for solving this problem, and it provides a reference for the dynamical analysis of similar nonlinear systems.

The role of CO_(2)dissociation in CO_(2)hydrogenation to ethanol on CoCu/silica catalysts

CoCu-based catalysts are widely used in CO_(x) hydrogenation reactions to produce higher alcohols due to the C–C coupling ability of Co and the ability of Cu to produce alcohols.This work describes the role of easily happened CO_(2)dissociation on the CoCu surface during the reaction,using different silica support to tune the metal–support interaction,reaches different selectivity to ethanol.CoCu supported on mesoporous silica MCM-41 shows ethanol selectivity as high as 85.3%,the ethanol space-time yield(STY)is 0.229 mmol/(gmetal∙h),however,poor selectivity to ethanol as low as 28.8%is observed on CoCu supported on amorphous silica.The different selectivity is due to the different intensities of CO_(2)dissociation on the catalysts.The adsorbed O*produced via CO_(2)dissociation can occupy the cobalt hollow sites on CoCu surfaces,which are also the adsorption sites of C1 intermediates for further C–C coupling.

Analysis on a Severe Convective Weather Process of Guangxi in 2018

Based on conventional meteorological observation data and Doppler radar data,the occurrence and development mechanism of mixed severe convective weather and evolution of convective storm in Guangxi on March 4,2018 were analyzed. The results showed that the dry line was the main trigger mechanism of this severe convective weather. Instable convection stratification of cold advection at middle layer and warm advection at low layer and abundant water vapor from low-level jet provided favorable stratification and water vapor conditions for the occurrence and development of severe convection. Cold trough at middle layer,low pressure and strong vertical wind shear at middle and lower layers may be main factors for the development and maintenance of strong storm system. Squall line developed along ground convergence line,and there was bow echo on reflectivity factor chart. Moving velocity of convective system was quick,and there was gale core and velocity ambiguity on velocity map.

Prognostic significance of clinicopathologic parameters in gastrointestinal stromal tumors:a study of 156 cases

The biological behavior of gastrointestinal stromal tumors(GISTs)are highly variable.To invest-igate the biological behavior of GIST,we collected 83 cases of gastric and 62 cases of small intestinal GIST from the Department of Pathology of the Chinese PLA General Hospital.The parameters include age,primary tumor location,tumor diameter,mitotic index,tumor necrosis,risk assessment,clinical stage and the c-kit exon 11 muta-tion.All these were analyzed in 105 cases along with the follow-up data and tested by log rank and COX hazard proportional model.We find that the average age of gast-ric GIST was 55.4 years.Of the 62 cases that were fol-lowed up,17 cases had metastasis or recurrence and the 5-year survival rate was(66.51±17.06)%.For the small intestinal GIST,the average age was 50.6 years and 43 cases were followed up.Of these,22 cases had meta-stasis or recurrence and the 5-year survival rate was(61.76±18.30)%.Small intestinal GIST was more fre-quently associated with metastasis and tumor relapse than gastric GIST(x^(2)=56.131,P=0.013).For gastric GIST,patients younger than 50 years(P=0.046),the advanced clinical stage(P=0.0001),the large tumor diameter(P=0.0001),a high mitotic index(P=0.0001),necrosis(P=0.0001)and a high risk grade(P=0.004)were all correlated with a lower survival rate.The COX hazard proportional model revealed that advanced clinical stage(P=0.001),large tumor size(P=0.001),a high mitotic index(P=0.002)and the high risk grade(P=0.018)indi-cated a poorer prognosis in gastric GIST.For small intest-inal GIST,necrosis(P=0.036)and advanced clinical stage(P=0.010)were associated with lower survival rates and the clinical stage was shown to be an independent prognostic indicator.A total of 25 cases harbored muta-tions in c-kit exon 11.The frequency of c-kit mutation was 32%and 22.5%for gastric and small intestinal GIST,respectively.In gastric GIST,the mutated c-kit was pre-dominant in patients older than 50 years of age.But in the small intestinal GIST,the mutated c-kit was predominant in the age group of 40-49 years.In conclusion,for gastric GIST,clinical stage,tumor size,mitotic index,and risk grade are the prognostic indicators.For small intestinal GIST,necrosis and clinical stage are the prognostic indi-cators.Small intestinal GIST are more aggressive than gastric GIST.The occurrence of c-kit mutation may cor-relate with the age of patients.

Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis

Gastric cancer（GC） is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development,in this study, we used 22 K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles of GC in 45 tissue samples, including 20 intestinal GC tissue samples,20 normal appearing tissues（NATs） adjacent to tumors and 5 noncancerous gastric mucosa tissue samples. These profiles allowed us to explore the transcriptional characteristics of GC and determine the change patterns in gene expression that may be of clinical significance. 1519 and 1255 differentially-expressed genes（DEGs） were identified in intestinal GC tissues and NATs, respectively,as determined by Bayesian analysis（P 〈 0.001）. These genes were associated with diverse functions such as mucosa secretion, metabolism, proliferation, signaling and development, which occur at different stages of GC development.

Cluster oscillation and bifurcation of fractional-order Duffing system with two time scales

The dynamical behavior on fractional-order Duffing system with two time scales is investigated,and the point-cycle coupling type cluster oscillation is firstly observed herein.When taking the fractional order as bifurcation parameter,the dynamics of the autonomous Duffing system will become more complex than the corresponding integer-order one,and some typical phenomenon exist only in the fractional-order one.Different attractors exist in various parameter space,and Hopf bifurcation only happens while fractional order is bigger than 1 under certain parameter condition.Moreover,the bifurcation behavior of the autonomous system may regulate dynamical phenomenon of the periodic excited system.It results into the point-cycle coupling type cluster oscillation when the fractional order is bigger than 1.The related generation mechanism based on slow-fast analysis method is that the slow variation of periodic excitation makes the system periodically visit different attractors and critical points of different bifurcations of the autonomous system.

A High-Altitude Platform Air-Ground Wireless Communication System Based on Beidou

Aerostat platforms could serve as potential aerial platforms because of their low cost,long flight times,and easy load recovery.They are suitable for emergency communication,early warnings,Earth observations,and other fields.The air-ground communication link between an aerostat platform and the ground is the basic guarantee for aerostat measurement control and service transmission.In traditional aerostat applications,platform measurement control and service transmission usually use different links.The load and ground equipment are complex,and the energy,load,and size of the platform are greatly occupied.In this paper,we propose a Beidou-assisted air-ground communication ground for a near-space platform that uses Beidou's positioning and communication functions to provide tracking and guidance information for the ground station.An air-ground wireless communication mode integrating measurement control and service transmission is achieved.The flight test shows that the system can effectively meet the practical application requirements.