[ Objective ] This study aimed to verify the feasibility of in silico cloning of functional candidate genes in tea. [ Method ] Theobroma cacao caffeine syn- thase gene BCS1 was used as a probe to search the establishe...[ Objective ] This study aimed to verify the feasibility of in silico cloning of functional candidate genes in tea. [ Method ] Theobroma cacao caffeine syn- thase gene BCS1 was used as a probe to search the established tea EST database using BLAST; 26 tea ESTs highly homologous to BCS1 were obtained, which were assembled using CAP (contig assembly program) of BioEdit software; subsequently, two EST configs harboring ORF were obtained, which were named TCSnewl and TCSnew2, respectively. Nucleotide sequences and deduced amino acid sequences of theses two genes were compared with those of cDNA of tea caffeine synthase gene TCS in the GenBank database that was cloned with experimental biological method. A phylogenetic tree was constructed for homalogous analysis of the deduced amino acid sequences of theses three genes. [ Result] in silico cloning of functional candidate genes in tea using a homologous gene of distantly related species as a probe is a feasible technical means. [ Conclusion] This study provided the basis for in silico cloning of other functional genes in tea.展开更多
In this paper, we consider the norms related to spectral geometric means and geometric means. When A and B are positive and invertible, we have ||A<sup>-1</sup>#B|| ≤ ||A<sup>-1</sup>σ<sub...In this paper, we consider the norms related to spectral geometric means and geometric means. When A and B are positive and invertible, we have ||A<sup>-1</sup>#B|| ≤ ||A<sup>-1</sup>σ<sub>s</sub>B||. Let H be a Hilbert space and B(H) be the set of all bounded linear operators on H. Let A ∈ B(H). If ||A#X|| = ||Aσ<sub>s</sub>X||, ?X ∈ B(H)<sup>++</sup>, then A is a scalar. When is a C*-algebra and for any , we have that ||logA#B|| = ||logAσ<sub>s</sub>B||, then is commutative.展开更多
A problem of video streaming of scalable video coding(SVC)is studied in vehicular networks.To improve the performance of the video streaming services and alleviate the pressure on backhaul links,the small cell base st...A problem of video streaming of scalable video coding(SVC)is studied in vehicular networks.To improve the performance of the video streaming services and alleviate the pressure on backhaul links,the small cell base stations(SBS)is proposed with caching ability to assist the content delivery.In this paper,it introduced the problem of joint optimization of caching strategy and transmission path in SBS cache cluster.An integer programming problem was formulated to maximize the average quality of experience.In order to obtain the globally optimal solution,the primal problem was first relaxed,then an adaptive algorithm was used based on the joint KKT condition,and the branch definition algorithm was applied.Extensive simulations were performed to demonstrate the efficiency of our proposed caching strategy.展开更多
基金Supported by National Science and Technology Support Program of China(2011BAD01B01)Youth Talent Innovation Fund of Fujian Academy of Agricultural Sciences(2011QC-2)Special Fund for"Double Hundred Plan"of Fujian Academy of Agricultural Sciences(sbmx1303-1)
文摘[ Objective ] This study aimed to verify the feasibility of in silico cloning of functional candidate genes in tea. [ Method ] Theobroma cacao caffeine syn- thase gene BCS1 was used as a probe to search the established tea EST database using BLAST; 26 tea ESTs highly homologous to BCS1 were obtained, which were assembled using CAP (contig assembly program) of BioEdit software; subsequently, two EST configs harboring ORF were obtained, which were named TCSnewl and TCSnew2, respectively. Nucleotide sequences and deduced amino acid sequences of theses two genes were compared with those of cDNA of tea caffeine synthase gene TCS in the GenBank database that was cloned with experimental biological method. A phylogenetic tree was constructed for homalogous analysis of the deduced amino acid sequences of theses three genes. [ Result] in silico cloning of functional candidate genes in tea using a homologous gene of distantly related species as a probe is a feasible technical means. [ Conclusion] This study provided the basis for in silico cloning of other functional genes in tea.
文摘In this paper, we consider the norms related to spectral geometric means and geometric means. When A and B are positive and invertible, we have ||A<sup>-1</sup>#B|| ≤ ||A<sup>-1</sup>σ<sub>s</sub>B||. Let H be a Hilbert space and B(H) be the set of all bounded linear operators on H. Let A ∈ B(H). If ||A#X|| = ||Aσ<sub>s</sub>X||, ?X ∈ B(H)<sup>++</sup>, then A is a scalar. When is a C*-algebra and for any , we have that ||logA#B|| = ||logAσ<sub>s</sub>B||, then is commutative.
基金the System Architecture Project(No.614000-40503)the Natural Science Foundation of China(No.61872104)+1 种基金the Natural Science Foundationof Heilongjiang Province in China(No.F2016028)the Fundamental Research Fund forthe Central Universities in China,and Tianjin Key Laboratory of Advanced Networking(TANK)in College of Intelligence and Computing of Tianjin University。
文摘A problem of video streaming of scalable video coding(SVC)is studied in vehicular networks.To improve the performance of the video streaming services and alleviate the pressure on backhaul links,the small cell base stations(SBS)is proposed with caching ability to assist the content delivery.In this paper,it introduced the problem of joint optimization of caching strategy and transmission path in SBS cache cluster.An integer programming problem was formulated to maximize the average quality of experience.In order to obtain the globally optimal solution,the primal problem was first relaxed,then an adaptive algorithm was used based on the joint KKT condition,and the branch definition algorithm was applied.Extensive simulations were performed to demonstrate the efficiency of our proposed caching strategy.