China's crop structure has undergone significant changes in the last two decades since 2000,with an increase in the share of cereals,vegetables,and fruit,squeezing out other crops.As a result,land productivity,nut...China's crop structure has undergone significant changes in the last two decades since 2000,with an increase in the share of cereals,vegetables,and fruit,squeezing out other crops.As a result,land productivity,nutrient supply,and carbon emissions have changed.How to reallocate limited farmland among crops to achieve the multiple goals of agrifood systems becomes an important issue.This study explores the sources of land productivity and nutrition supply growth and carbon emissions reduction,and identifies the multiple roles of crop structural change from 2003 to 2020 based on a decomposition analysis.The results reveal that the growth within crops is still the primary driver in land productivity and nutrition supply and the reduction in carbon emissions.However,structural change also plays various roles at different periods.From 2003 to 2010,crop structural change increased the total calorie supply but lowered land productivity and contributed at least 70%of the total growth of carbon emissions.The crop structure was relatively stable,and their effects were modest from 2010 to 2015.From 2015 to 2020,the crop structural change began to play a greater role and generate synergistic effects in improving land productivity,micronutrient supply,and reducing carbon emissions,contributing to approximately a quarter of the growth of land productivity and 30%of total carbon emissions reduction.These results suggest that strategies for crop structural change should comprehensively consider its multiple impacts,aiming to achieve co-benefits while minimizing trade-offs.展开更多
Rechargeable Zn-air batteries(ZAB)have drawn extensive attention due to their eco-friendliness and safety.However,the lack of high-performance and low-cost oxygen redox reactions(OER and ORR)catalysts has become one o...Rechargeable Zn-air batteries(ZAB)have drawn extensive attention due to their eco-friendliness and safety.However,the lack of high-performance and low-cost oxygen redox reactions(OER and ORR)catalysts has become one of the main stumbling blocks in their development.Herein,we successfully fabricate a CoFe nanobubble encapsulated in nitrogen-doped carbon nanocage on wood carbon support(CoFe@NC/WC)via pyrolysis of a novel Prussian blue analog(PBA)/spruce precursor.The hierarchical CoFe@NC/WC catalyst exhibits an excellent potential difference of 0.74 V between the OER potential at 10 mA cm^(-2)and half-wave potential of ORR in 0.1 M KOH,comparable to recently reported preeminent electrocatalysts.Further,CoFe@NC/WC shows outstanding electrochemical performance in liquid ZAB,with a peak power density of 138.9 mW cm^(-2)and a specific capacity of 763.5 mAh g^(-1).More importantly,a bacterial cellulose nanofiber reinforced polyacrylic acid(BC-PAA)hydrogel electrolyte shows ultrahigh tensile-breaking stress of 1.58 MPa.In conjunction with the as-prepared CoFe@NC/WC catalyst,BC-PAA-based wearable ZAB displays impressive rechargeability and foldability,and can power portable electronics,such as electronic timer and mobile phone,in bent states.This work provides a new approach toward high-activity and low-cost catalysts for ZAB.展开更多
To the editor:Mood disorders(MD)are serious mental illnesses that commonly affect adolescents,leading to a high incidence of suicidal behaviour.1 In China,the suicide attempt(SA)rate for adolescents with MD is 51.96%,...To the editor:Mood disorders(MD)are serious mental illnesses that commonly affect adolescents,leading to a high incidence of suicidal behaviour.1 In China,the suicide attempt(SA)rate for adolescents with MD is 51.96%,2 and over 500000 adolescent SA are reported annually in the USA due to depression.3 Risk factors for SA include gender,hormone levels,family conflict and,particularly,negative cognitive styles such as rumination.展开更多
Totheeditor:Major depressive disorder(MDD)is a principal cause of disability worldwide and is often associated with high morbidity and mortality rates.Although there are several therapies available for the treatment o...Totheeditor:Major depressive disorder(MDD)is a principal cause of disability worldwide and is often associated with high morbidity and mortality rates.Although there are several therapies available for the treatment of depression,about one-third of patients with MMD will not respond to two or more antidepressant drugs with different mechanisms;the patients are then referred to as having treatment-resistant depression(TRD).Patients with TRD have a poorer quality of life,greater economic burden and increased suicidal behaviours.Therefore,new antidepressant treatments that are effective,safe,long-lasting and tolerable are needed.Ketamine infusion,intranasal esketamine and transcranial magnetic stimulation(TMS)have been used to treat early stage TRD.’A recent review suggests that electroconvulsive therapy(ECT)may be superior to ketamine for reducing depression severity in the acute treatment of TRD.展开更多
A comprehensive understanding of the failure behavior and mechanism of coal is a prerequisite for dealing with dynamic problems in mining space.In this study,the failure behavior and mechanism of coal under uniaxial d...A comprehensive understanding of the failure behavior and mechanism of coal is a prerequisite for dealing with dynamic problems in mining space.In this study,the failure behavior and mechanism of coal under uniaxial dynamic compressive loads were experimentally and numerically investigated.The experiments were conducted using a split Hopkinson pressure bar(SHPB)system.The results indicated that the typical failure of coal is lateral and axial at lower loading rates and totally smashed at higher loading rates.The further fractography analysis of lateral and axial fracture fragments indicated that the coal failure under dynamic compressive load is caused by tensile brittle fracture.In addition,the typical failure modes of coal under dynamic load were numerically reproduced.The numerical results indicated that the axial fracture is caused directly by the incident compressive stress wave and the lateral fracture is caused by the tensile stress wave reflected from the interface between coal specimen and transmitted bar.Potential application was further conducted to interpret dynamic problems in underground coal mine and it manifested that the lateral and axial fractures of coal constitute the parallel cracks in the coal mass under roof fall and blasting in mining space.展开更多
A mixture of fault gouge and rubble taken out from a fault zone is used to prepare a S-RM(Soil-Rock Mixture)sample with rock block proportions of 20%,30%,40%,50%,60%and 70%,respectively.A GDS triaxial test system is u...A mixture of fault gouge and rubble taken out from a fault zone is used to prepare a S-RM(Soil-Rock Mixture)sample with rock block proportions of 20%,30%,40%,50%,60%and 70%,respectively.A GDS triaxial test system is used accordingly to measure the seepage characteristics of such samples under different loading and unloading confining pressures in order to determine the variation law of the permeability coefficient.The test results show that:(1)The permeability coefficient of the S-RM samples decreases as the pressure increases,and the decrease rate of this coefficient in the initial stage of confining pressure loading is obviously higher than in the semi-late period;(2)The permeability coefficient at different confining pressure levels presents a common trend as the rock block proportion is increased,i.e.,it decreases first then it increases(the permeability coefficient of the sample with rock block proportion 40%being the smallest,70%the largest);(3)In the stage of confining pressure unloading,the recovery degree of the permeability coefficient grows with the increase of rock block proportion(the recovery rate of S-RM sample with rock block proportion 70%reaches 50.2%);(4)In the stage of confining pressure loading and unloading,the sensitivity of the permeability coefficient to the rock block proportion displays the inverse“Z”variation rule(when rock block proportion reaches 60%,the sensitivity is highest);(5)In the stage of confining pressure loading,the relationship between the permeability coefficient and confining pressure can be described by an exponential relationship.展开更多
For the car sequencing（CS） problem, the draw-backs of the ＂sliding windows＂ technique used in the objective function have not been rectified, and no high quality initial solution has been acquired to accelerate th...For the car sequencing（CS） problem, the draw-backs of the ＂sliding windows＂ technique used in the objective function have not been rectified, and no high quality initial solution has been acquired to accelerate the improvement of the solution quality. Firstly, the objective function is improved to solve the double and bias counting of violations broadly discussed. Then, a new method combining heuristic with constraint propagation is proposed which constructs initial solutions under a parallel framework. Based on constraint propagation, three filtering rules are designed to intersecting with three greedy functions, so the variable domain is narrowed in the process of the construction. The parallel framework is served to show its robustness in terms of the quality of the solution since it greatly increases the performance of obtaining the best solution. In the computational experiments, 109 instances of 3 sets from the CSPLib＇ s benchmarks are used to test the performance of the proposed method. Experiment results show that the proposed method outperforms others in acquiring the best-known results for 85 best-known results of 109 are obtained with only one construction. The proposed research provides an avenue to remedy the deficiencies of ＂sliding windows＂ technique and construct high quality initial solutions.展开更多
Target modeling and scattering function calculating are important prerequisites and groundwork for the synthetic aperture radar（SAR） imaging simulation.According to the difficult problems that normal methods cannot ...Target modeling and scattering function calculating are important prerequisites and groundwork for the synthetic aperture radar（SAR） imaging simulation.According to the difficult problems that normal methods cannot calculate the scattering function of electrically large object under the condition to wideband,an effective method of improved equivalent edge currents is presented and applied to SAR imaging simulation for the first time.This method improves calculating velocity and has relatively high precision.The concrete steps of applying the method are given.By way of the simulation experiment,the effectiveness of the method is verified.展开更多
Metal oxide and carbide strengthening molybdenum(Mo)alloys have been designed as promising ad-vanced materials in refractory metals to solve some of the core engineering problems in superalloy ap-plications.Hence,ther...Metal oxide and carbide strengthening molybdenum(Mo)alloys have been designed as promising ad-vanced materials in refractory metals to solve some of the core engineering problems in superalloy ap-plications.Hence,there is a need to summarize the results obtained and evaluate the opportunities for preparing high-performance Mo alloys by strengthening metal oxides and carbides to improve the per-formance characteristics of Mo metal materials.This paper reviews the results of the reported work con-cerning the structure and properties of Mo alloys with different metal oxide and carbide strengthening methods added to Mo matrix.The influence of the doping of La 2 O 3 and Y 2 O 3 particles,ceramic Al 2 O 3 and ZrO 2 particles,and refractory TiC and ZrC carbides particles of Mo alloys are discussed.The impacts of particle morphology,size,distribution and volume fractions of oxide and carbide are analyzed,as well as the specific features of different doping techniques for obtaining high-performance Mo alloys mate-rials.This work will guide future research on the design of high-performance refractory Mo alloys by adding oxides and carbide particles,helping to solve the core issues in the field of superalloy application research.展开更多
OBJECTIVE： To ascertain if it is effective to use extradural injections and manual repositioning of the spine to treat megalgia caused by cervical spondy- Iotic radiculopathy （CSR）. METHODS： Patients with megalgia...OBJECTIVE： To ascertain if it is effective to use extradural injections and manual repositioning of the spine to treat megalgia caused by cervical spondy- Iotic radiculopathy （CSR）. METHODS： Patients with megalgia caused by CSR were divided into a treatment group （n=46） treated by extradural injection and manual repositioning of the spine and a control group （n=46） treated by a conventional method. RESULTS： The prevalence of cure was 58.69% and the total prevalence of effective cure in the treatment group was 97.83% and was 23.91% and 78.26%, respectively, in the control group： this difference between the two groups was significant （P〈 0.05）. CONCLUSION： Treatment of megalgia caused by CSR by extradural injections and manual repositioning of the spine has a good curative effect with rapid analgesia and short therapeutic course.展开更多
Osteogenesis imperfecta（OI,also known as brittle bone disease）is caused mostly by mutations in two type I collagen genes,COL1A1 and COLIA2 encoding the pro-α1（I）and pro-α2（I）chains of type I collagen,respectiv...Osteogenesis imperfecta（OI,also known as brittle bone disease）is caused mostly by mutations in two type I collagen genes,COL1A1 and COLIA2 encoding the pro-α1（I）and pro-α2（I）chains of type I collagen,respectively.Two Chinese families with autosomal dominant OI were identified and characterized.Linkage analysis revealed linkage of both families to COL1A2 on chromosome 7q21.3-q22.1.Mutational analysis was carried out using direct DNA sequence analysis.Two novel missense mutations,c.3350AG and c.3305GC,were identified in exon 49 of COL1A2 in the two families,respectively.The c.3305GC mutation resulted in substitution of a glycine residue（G）by an alanine residue（A）at codon 1102（p.G1102A）,which was found to be mutated into serine（S）,argine（R）,aspartic acid（D）,or valine（V）in other families.The c.3350AG variant may be a de novo mutation resulting in p.Y1117C.Both mutations co-segregated with OI in respective families,and were not found in 100 normal controls.The G1102 and Y1117 residues were evolutionarily highly conserved from zebrafish to humans.Mutational analysis did not identify any mutation in the COX-2 gene（a modifier gene of OI）.This study identifies two novel mutations p.G1102A and p.Y1117C that cause OI,significantly expands the spectrum of COL1A2 mutations causing OI,and has a significant implication in prenatal diagnosis of OI.展开更多
基金This work was supported by the National Natural Science Foundation of China(72061147002 and 72373143)the National Social Science Fund of China(22&ZD085).
文摘China's crop structure has undergone significant changes in the last two decades since 2000,with an increase in the share of cereals,vegetables,and fruit,squeezing out other crops.As a result,land productivity,nutrient supply,and carbon emissions have changed.How to reallocate limited farmland among crops to achieve the multiple goals of agrifood systems becomes an important issue.This study explores the sources of land productivity and nutrition supply growth and carbon emissions reduction,and identifies the multiple roles of crop structural change from 2003 to 2020 based on a decomposition analysis.The results reveal that the growth within crops is still the primary driver in land productivity and nutrition supply and the reduction in carbon emissions.However,structural change also plays various roles at different periods.From 2003 to 2010,crop structural change increased the total calorie supply but lowered land productivity and contributed at least 70%of the total growth of carbon emissions.The crop structure was relatively stable,and their effects were modest from 2010 to 2015.From 2015 to 2020,the crop structural change began to play a greater role and generate synergistic effects in improving land productivity,micronutrient supply,and reducing carbon emissions,contributing to approximately a quarter of the growth of land productivity and 30%of total carbon emissions reduction.These results suggest that strategies for crop structural change should comprehensively consider its multiple impacts,aiming to achieve co-benefits while minimizing trade-offs.
基金supported by the Innovation and Technology Commission(Grant no.PRP/032/20FX)the RFBR(Grant no.20-03-00772)
文摘Rechargeable Zn-air batteries(ZAB)have drawn extensive attention due to their eco-friendliness and safety.However,the lack of high-performance and low-cost oxygen redox reactions(OER and ORR)catalysts has become one of the main stumbling blocks in their development.Herein,we successfully fabricate a CoFe nanobubble encapsulated in nitrogen-doped carbon nanocage on wood carbon support(CoFe@NC/WC)via pyrolysis of a novel Prussian blue analog(PBA)/spruce precursor.The hierarchical CoFe@NC/WC catalyst exhibits an excellent potential difference of 0.74 V between the OER potential at 10 mA cm^(-2)and half-wave potential of ORR in 0.1 M KOH,comparable to recently reported preeminent electrocatalysts.Further,CoFe@NC/WC shows outstanding electrochemical performance in liquid ZAB,with a peak power density of 138.9 mW cm^(-2)and a specific capacity of 763.5 mAh g^(-1).More importantly,a bacterial cellulose nanofiber reinforced polyacrylic acid(BC-PAA)hydrogel electrolyte shows ultrahigh tensile-breaking stress of 1.58 MPa.In conjunction with the as-prepared CoFe@NC/WC catalyst,BC-PAA-based wearable ZAB displays impressive rechargeability and foldability,and can power portable electronics,such as electronic timer and mobile phone,in bent states.This work provides a new approach toward high-activity and low-cost catalysts for ZAB.
基金This study was supported by the Planning Program of the Health Committee of Ganzhou(2022-1-5)the Key R&D Program of Jiangxi Province(20202BBGL73106)grants from the National Science Foundation of China(81771435).
文摘To the editor:Mood disorders(MD)are serious mental illnesses that commonly affect adolescents,leading to a high incidence of suicidal behaviour.1 In China,the suicide attempt(SA)rate for adolescents with MD is 51.96%,2 and over 500000 adolescent SA are reported annually in the USA due to depression.3 Risk factors for SA include gender,hormone levels,family conflict and,particularly,negative cognitive styles such as rumination.
基金supported by the National Natural Science Foundation of China(82371490)the National Key R&D Program of China(2022YFC2503901,2022YFC2503900)+1 种基金the Beijing Hundred,Thousand,and Ten Thousand Talents Project(2017-CXYF-09)Beijing Health System Leading Talent Grant(2022-02-10).
文摘Totheeditor:Major depressive disorder(MDD)is a principal cause of disability worldwide and is often associated with high morbidity and mortality rates.Although there are several therapies available for the treatment of depression,about one-third of patients with MMD will not respond to two or more antidepressant drugs with different mechanisms;the patients are then referred to as having treatment-resistant depression(TRD).Patients with TRD have a poorer quality of life,greater economic burden and increased suicidal behaviours.Therefore,new antidepressant treatments that are effective,safe,long-lasting and tolerable are needed.Ketamine infusion,intranasal esketamine and transcranial magnetic stimulation(TMS)have been used to treat early stage TRD.’A recent review suggests that electroconvulsive therapy(ECT)may be superior to ketamine for reducing depression severity in the acute treatment of TRD.
基金supports for this work,provided by the Natural Science Foundation of Anhui Province(No.1908085QE187,1808085ME161)the Open Research Program of Key Laboratory of Safety and High-efficiency Coal Mining(No.JYBSYS2019202)the Open Research Program of State Key Laboratory Cultivation Base for Gas Geology and Gas Control(No.WS2019B09)are gratefully acknowledged.
文摘A comprehensive understanding of the failure behavior and mechanism of coal is a prerequisite for dealing with dynamic problems in mining space.In this study,the failure behavior and mechanism of coal under uniaxial dynamic compressive loads were experimentally and numerically investigated.The experiments were conducted using a split Hopkinson pressure bar(SHPB)system.The results indicated that the typical failure of coal is lateral and axial at lower loading rates and totally smashed at higher loading rates.The further fractography analysis of lateral and axial fracture fragments indicated that the coal failure under dynamic compressive load is caused by tensile brittle fracture.In addition,the typical failure modes of coal under dynamic load were numerically reproduced.The numerical results indicated that the axial fracture is caused directly by the incident compressive stress wave and the lateral fracture is caused by the tensile stress wave reflected from the interface between coal specimen and transmitted bar.Potential application was further conducted to interpret dynamic problems in underground coal mine and it manifested that the lateral and axial fractures of coal constitute the parallel cracks in the coal mass under roof fall and blasting in mining space.
基金This work was supported by the Key Laboratory of Safety and High-Efficiency Coal Mining,Ministry of Education,Anhui University of Science and Technology(JYBSYS2020209)the Natural Science Research Project of Anhui Provincial Department of Education(KJHS2020B13)the Huangshan University School Level Talent Launch Project(No.2020XKJQ001).
文摘A mixture of fault gouge and rubble taken out from a fault zone is used to prepare a S-RM(Soil-Rock Mixture)sample with rock block proportions of 20%,30%,40%,50%,60%and 70%,respectively.A GDS triaxial test system is used accordingly to measure the seepage characteristics of such samples under different loading and unloading confining pressures in order to determine the variation law of the permeability coefficient.The test results show that:(1)The permeability coefficient of the S-RM samples decreases as the pressure increases,and the decrease rate of this coefficient in the initial stage of confining pressure loading is obviously higher than in the semi-late period;(2)The permeability coefficient at different confining pressure levels presents a common trend as the rock block proportion is increased,i.e.,it decreases first then it increases(the permeability coefficient of the sample with rock block proportion 40%being the smallest,70%the largest);(3)In the stage of confining pressure unloading,the recovery degree of the permeability coefficient grows with the increase of rock block proportion(the recovery rate of S-RM sample with rock block proportion 70%reaches 50.2%);(4)In the stage of confining pressure loading and unloading,the sensitivity of the permeability coefficient to the rock block proportion displays the inverse“Z”variation rule(when rock block proportion reaches 60%,the sensitivity is highest);(5)In the stage of confining pressure loading,the relationship between the permeability coefficient and confining pressure can be described by an exponential relationship.
基金Supported by National Natural Science Foundation of China(Grant Nos.51435009,71302085)Zhejiang Provincial Natural Science Foundation of China(Grant No.LQ14E080002)K.C.Wong Magna Fund in Ningbo University
文摘For the car sequencing（CS） problem, the draw-backs of the ＂sliding windows＂ technique used in the objective function have not been rectified, and no high quality initial solution has been acquired to accelerate the improvement of the solution quality. Firstly, the objective function is improved to solve the double and bias counting of violations broadly discussed. Then, a new method combining heuristic with constraint propagation is proposed which constructs initial solutions under a parallel framework. Based on constraint propagation, three filtering rules are designed to intersecting with three greedy functions, so the variable domain is narrowed in the process of the construction. The parallel framework is served to show its robustness in terms of the quality of the solution since it greatly increases the performance of obtaining the best solution. In the computational experiments, 109 instances of 3 sets from the CSPLib＇ s benchmarks are used to test the performance of the proposed method. Experiment results show that the proposed method outperforms others in acquiring the best-known results for 85 best-known results of 109 are obtained with only one construction. The proposed research provides an avenue to remedy the deficiencies of ＂sliding windows＂ technique and construct high quality initial solutions.
基金supported by the National Natural Science Foundation of China(60871070)
文摘Target modeling and scattering function calculating are important prerequisites and groundwork for the synthetic aperture radar（SAR） imaging simulation.According to the difficult problems that normal methods cannot calculate the scattering function of electrically large object under the condition to wideband,an effective method of improved equivalent edge currents is presented and applied to SAR imaging simulation for the first time.This method improves calculating velocity and has relatively high precision.The concrete steps of applying the method are given.By way of the simulation experiment,the effectiveness of the method is verified.
基金the Outstanding Doctorate Dis-sertation Cultivation Fund of Xi’an University of Architecture and Technology(No.160842012)ScientificandTechnologicalInnova-tion Team Project of the Shaanxi Innovation Capability Support Plan,China(No.2022TD-30)+8 种基金the Fok Ying Tung Education Foun-dation(No.171101)Youth Innovation Team of Shaanxi Universi-ties(No.2019-2022)Top young talents project of“Special support program for high-level talents”in the Shaanxi Province(No.2018-2023)Major scientific and technological projects in the Shaanxi Province of China(No.2020ZDZX04-02-01)Service local spe-cial program of education department of Shaanxi province,China(No.21JC016)General Special Scientific Research Program of the Shaanxi Provincial Department of Education(No.21JK0722)the General Projects of Key R&D Program of the Shaanxi Province,China(No.2021GY-209)China Postdoctoral Science Foundation(No.2021M693878)China Postdoctoral Science Foundation(No.2021MD703866).
文摘Metal oxide and carbide strengthening molybdenum(Mo)alloys have been designed as promising ad-vanced materials in refractory metals to solve some of the core engineering problems in superalloy ap-plications.Hence,there is a need to summarize the results obtained and evaluate the opportunities for preparing high-performance Mo alloys by strengthening metal oxides and carbides to improve the per-formance characteristics of Mo metal materials.This paper reviews the results of the reported work con-cerning the structure and properties of Mo alloys with different metal oxide and carbide strengthening methods added to Mo matrix.The influence of the doping of La 2 O 3 and Y 2 O 3 particles,ceramic Al 2 O 3 and ZrO 2 particles,and refractory TiC and ZrC carbides particles of Mo alloys are discussed.The impacts of particle morphology,size,distribution and volume fractions of oxide and carbide are analyzed,as well as the specific features of different doping techniques for obtaining high-performance Mo alloys mate-rials.This work will guide future research on the design of high-performance refractory Mo alloys by adding oxides and carbide particles,helping to solve the core issues in the field of superalloy application research.
文摘OBJECTIVE： To ascertain if it is effective to use extradural injections and manual repositioning of the spine to treat megalgia caused by cervical spondy- Iotic radiculopathy （CSR）. METHODS： Patients with megalgia caused by CSR were divided into a treatment group （n=46） treated by extradural injection and manual repositioning of the spine and a control group （n=46） treated by a conventional method. RESULTS： The prevalence of cure was 58.69% and the total prevalence of effective cure in the treatment group was 97.83% and was 23.91% and 78.26%, respectively, in the control group： this difference between the two groups was significant （P〈 0.05）. CONCLUSION： Treatment of megalgia caused by CSR by extradural injections and manual repositioning of the spine has a good curative effect with rapid analgesia and short therapeutic course.
基金supported by the China Natural Science Foundation grants (Nos. 30670736 and 30972655)the National Basic Research Program of China (973 Program) (No. 2007CB512002).
文摘Osteogenesis imperfecta（OI,also known as brittle bone disease）is caused mostly by mutations in two type I collagen genes,COL1A1 and COLIA2 encoding the pro-α1（I）and pro-α2（I）chains of type I collagen,respectively.Two Chinese families with autosomal dominant OI were identified and characterized.Linkage analysis revealed linkage of both families to COL1A2 on chromosome 7q21.3-q22.1.Mutational analysis was carried out using direct DNA sequence analysis.Two novel missense mutations,c.3350AG and c.3305GC,were identified in exon 49 of COL1A2 in the two families,respectively.The c.3305GC mutation resulted in substitution of a glycine residue（G）by an alanine residue（A）at codon 1102（p.G1102A）,which was found to be mutated into serine（S）,argine（R）,aspartic acid（D）,or valine（V）in other families.The c.3350AG variant may be a de novo mutation resulting in p.Y1117C.Both mutations co-segregated with OI in respective families,and were not found in 100 normal controls.The G1102 and Y1117 residues were evolutionarily highly conserved from zebrafish to humans.Mutational analysis did not identify any mutation in the COX-2 gene（a modifier gene of OI）.This study identifies two novel mutations p.G1102A and p.Y1117C that cause OI,significantly expands the spectrum of COL1A2 mutations causing OI,and has a significant implication in prenatal diagnosis of OI.
