BACKGROUND Genetic factors play an important role in the pathogenesis of panic disorder(PD).However,the effect of genetic variants on PD remains controversial.AIM To evaluate the associations between glutamate decarbo...BACKGROUND Genetic factors play an important role in the pathogenesis of panic disorder(PD).However,the effect of genetic variants on PD remains controversial.AIM To evaluate the associations between glutamate decarboxylase 1(GAD1)gene polymorphisms and PD risk and assess the effect of GAD1 gene polymorphisms on the severity of clinical symptoms in PD.METHODS We recruited 230 PD patients and 224 healthy controls in this study.All participants were assessed for anxiety and panic symptom severity using the Hamilton Anxiety Rating Scale(HAM-A)and Panic Disorder Severity Scale(PDSS).GAD1 gene polymorphisms(rs1978340 and rs3749034)were genotyped and assessed for allele frequencies.RESULTS There were no significant differences between cases and controls in the genotype distributions or allele frequencies of GAD1(rs1978340 and rs3749034).In addition,the effect of GAD1(rs1978340 and rs3749034)on PD severity was not significant.However,regarding respiratory symptoms,patients with the GAD1 rs1978340 A/A genotype had significantly higher scores than those with the A/G or G/G genotype.CONCLUSION Here,we showed that the A/A genotype of GAD1 rs1978340 was associated with increased severity of respiratory symptoms in patients with PD.展开更多
To prepare the PrP specific monoclonal antibodies （mAbs） that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinan...To prepare the PrP specific monoclonal antibodies （mAbs） that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein （HaPrP）. Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrP^Sc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPc from several other mammalian species, including humans and cattles, lmmunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPso. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.展开更多
Hepatic fibrosis(HF)is the common pathological basis of all chronic liver diseases,and is a necessary stage for the development of chronic liver disease into cirrhosis.The pathogenesis of liver fibrosis is very compli...Hepatic fibrosis(HF)is the common pathological basis of all chronic liver diseases,and is a necessary stage for the development of chronic liver disease into cirrhosis.The pathogenesis of liver fibrosis is very complicated.Different cells and cytokines play a major role in the pathogenesis of liver fibrosis.This article focuses on the development mechanism of liver fibrosis,different cells and cytokines(macrophages,natural killer cells,natural killer T cells,tumor necrosis factor,IL-22,transforming growth factor-β,connective tissue growth factor,vascular endothelial growth factor)Its impact on the development of liver fibrosis is reviewed.展开更多
Resource sharing, multi-tenant interference and bursty workloads in cloud computing lead to high tail-latency that severely affects user quality of experience （QoE）, where response latency is a critical factor. A lo...Resource sharing, multi-tenant interference and bursty workloads in cloud computing lead to high tail-latency that severely affects user quality of experience （QoE）, where response latency is a critical factor. A lot of research efforts are dedicated to reducing high tail-latency and improving user QoE, such as software-defined cloud computing （SDC）. However, the traditional availability analysis of cloud computing captures the pure failure-repair behavior with user QoE ignored. In this paper, we propose a conceptual framework, experience availability, to properly assess the effectiveness of SDC while taking into account both availability and response latency simultaneously. We review the related work on availability models and methods of cloud systems, and discuss open problems for evaluating experience availability in SDC. We also show some of our preliminary results to demonstrate the feasibility of our ideas.展开更多
In this study, we present a constructive algorithm for training cooperative support vector machine ensembles （CSVMEs）. CSVME combines ensemble architecture design with cooperative training for individual SVMs in ens...In this study, we present a constructive algorithm for training cooperative support vector machine ensembles （CSVMEs）. CSVME combines ensemble architecture design with cooperative training for individual SVMs in ensembles. Unlike most previous studies on training ensembles, CSVME puts emphasis on both accuracy and collaboration among individual SVMs in an ensemble. A group of SVMs selected on the basis of recursive classifier elimination is used in CSVME, and the number of the individual SVMs selected to construct CSVME is determined by 10-fold cross-validation. This kind of SVME has been tested on two ovarian cancer datasets previously obtained by proteomic mass spectrometry. By combining several individual SVMs, the proposed method achieves better performance than the SVME of all base SVMs.展开更多
With the multi-tier pricing scheme provided by most of the cloud service providers （CSPs）, the cloud userstypically select a high enough transmission service level to ensure the quality of service （QoS）, due to th...With the multi-tier pricing scheme provided by most of the cloud service providers （CSPs）, the cloud userstypically select a high enough transmission service level to ensure the quality of service （QoS）, due to the severe penalty ofmissing the transmission deadline. This leads to the so-called over-provisioning problem, which increases the transmissioncost of the cloud user. Given the fact that cloud users may not be aware of their traffic demand before accessing the network,the over-provisioning problem becomes more serious. In this paper, we investigate how to reduce the transmission cost fromthe perspective of cloud users, especially when they are not aware of their traffic demand before the transmission deadline.The key idea is to split a long-term transmission request into several short ones. By selecting the most suitable transmissionservice level for each short-term request, a cost-efiqcient inter-datacenter transmission service level selection framework isobtained. We further formulate the transmission service level selection problem as a linear programming problem andresolve it in an on-line style with Lyapunov optimization. We evaluate the proposed approach with real traffic data. Theexperimental results show that our method can reduce the transmission cost by up to 65.04%.展开更多
The aligned molecular packing structure is vital to the anisotropic charge transport in conjugated polymer and small molecule thin films.However,how this molecular packing motif influences the photoelectric conversion...The aligned molecular packing structure is vital to the anisotropic charge transport in conjugated polymer and small molecule thin films.However,how this molecular packing motif influences the photoelectric conversion process at the donor/acceptor heterojunction is still mysterious.Herein,we employed a PM6/Y6 bilayer model to investigate the long-range alignment of molecular packing induced photoelectric conversion process.Both PM6 and Y6 layers were properly controlled to exhibit the uniaxially oriented molecular packing compared to their as-cast counterparts,as revealed by the polarized absorption spectra and transmission electron microscopy.After analyzing the photovoltaic performance of bilayer devices,the smaller energy loss,lower energetic disorder,and longer charge carrier lifetime can be observed in the bilayer devices with aligned Y6 molecules,which contribute to a higher power conversion efficiency(PCE)than the as-cast devices.While the molecular packing structure of PM6 layer exhibited negligible influence on the device performance,probably resulting from the intrinsic semicrystalline nature of PM6 molecules.Our results indicate that the alignment of small molecular acceptor at the donor/acceptor interfaces should be a powerful strategy to facilitate the photoelectric conversion process,which will definitely pave the way to highly efficient bulk heterojunction photovoltaic device.展开更多
Proper development of the human placenta is of vital importance for a successful pregnancy,and a series of pregnancy complications are considered originating from dysfunctional placentas.Like other organ system develo...Proper development of the human placenta is of vital importance for a successful pregnancy,and a series of pregnancy complications are considered originating from dysfunctional placentas.Like other organ system development,placentation requires large numbers of co-regulators,while the underlying molecular mechanisms orchestrating the placental formation and function are poorly understood.Although we have made many signs of progress in understanding the placental architectures and developments using mouse models,the species-specific differences impede our progress due to the lack of appropriate model systems.In the past few years,major progress has been made by the establishment of novel in-vitro self-renewing stem cell models,as well as identifying the full picture of the cellular organization of the maternal and fetal interface.Providing the tools for the investigation of placentation and reproductive-related regulation mechanism.In this review,we focus on the detailed progress of the human trophoblast stem cells culturing system,and the cellular and molecular terrain at the maternal-fetal interface,respectively,thus providing new insights into placental development.展开更多
文摘BACKGROUND Genetic factors play an important role in the pathogenesis of panic disorder(PD).However,the effect of genetic variants on PD remains controversial.AIM To evaluate the associations between glutamate decarboxylase 1(GAD1)gene polymorphisms and PD risk and assess the effect of GAD1 gene polymorphisms on the severity of clinical symptoms in PD.METHODS We recruited 230 PD patients and 224 healthy controls in this study.All participants were assessed for anxiety and panic symptom severity using the Hamilton Anxiety Rating Scale(HAM-A)and Panic Disorder Severity Scale(PDSS).GAD1 gene polymorphisms(rs1978340 and rs3749034)were genotyped and assessed for allele frequencies.RESULTS There were no significant differences between cases and controls in the genotype distributions or allele frequencies of GAD1(rs1978340 and rs3749034).In addition,the effect of GAD1(rs1978340 and rs3749034)on PD severity was not significant.However,regarding respiratory symptoms,patients with the GAD1 rs1978340 A/A genotype had significantly higher scores than those with the A/G or G/G genotype.CONCLUSION Here,we showed that the A/A genotype of GAD1 rs1978340 was associated with increased severity of respiratory symptoms in patients with PD.
文摘To prepare the PrP specific monoclonal antibodies （mAbs） that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein （HaPrP）. Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrP^Sc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPc from several other mammalian species, including humans and cattles, lmmunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPso. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.
基金General program of national natural science foundation of China(No.81774236)Innovation research team project of science and technology department,Guangxi Zhuang autonomous region(No.2018GXNSFGA281002)Graduate education innovation project of Guangxi Zhuang autonomous region(No.YCSY201900117)。
文摘Hepatic fibrosis(HF)is the common pathological basis of all chronic liver diseases,and is a necessary stage for the development of chronic liver disease into cirrhosis.The pathogenesis of liver fibrosis is very complicated.Different cells and cytokines play a major role in the pathogenesis of liver fibrosis.This article focuses on the development mechanism of liver fibrosis,different cells and cytokines(macrophages,natural killer cells,natural killer T cells,tumor necrosis factor,IL-22,transforming growth factor-β,connective tissue growth factor,vascular endothelial growth factor)Its impact on the development of liver fibrosis is reviewed.
基金This work is supported by the National Key Research and Development Program of China under Grant No. 2016YFB1000205, the National Natural Science Foundation of China under Grant No. 61402325, and the Tianjin City Application Foundation and Cutting- Edge Technology Research Program under Grant No. 14JCQNJC00500.
文摘Resource sharing, multi-tenant interference and bursty workloads in cloud computing lead to high tail-latency that severely affects user quality of experience （QoE）, where response latency is a critical factor. A lot of research efforts are dedicated to reducing high tail-latency and improving user QoE, such as software-defined cloud computing （SDC）. However, the traditional availability analysis of cloud computing captures the pure failure-repair behavior with user QoE ignored. In this paper, we propose a conceptual framework, experience availability, to properly assess the effectiveness of SDC while taking into account both availability and response latency simultaneously. We review the related work on availability models and methods of cloud systems, and discuss open problems for evaluating experience availability in SDC. We also show some of our preliminary results to demonstrate the feasibility of our ideas.
基金partly supported by the National Natural Science Foundation of China (No.60574019 and 60474045)the National Basic Research Program(973 Program)of China(No.2002CB312200)+2 种基金the Key Technologies R&D Program of Zhejiang Province (No.2005C21087)the Academician Foundation of Zhejiang Province(No.2005A1001-13)the Center for Bioinformatics Program Grant of Harvard Center of Neurodegeneration and Repair,Harvard Medical School,Boston,USA.
文摘In this study, we present a constructive algorithm for training cooperative support vector machine ensembles （CSVMEs）. CSVME combines ensemble architecture design with cooperative training for individual SVMs in ensembles. Unlike most previous studies on training ensembles, CSVME puts emphasis on both accuracy and collaboration among individual SVMs in an ensemble. A group of SVMs selected on the basis of recursive classifier elimination is used in CSVME, and the number of the individual SVMs selected to construct CSVME is determined by 10-fold cross-validation. This kind of SVME has been tested on two ovarian cancer datasets previously obtained by proteomic mass spectrometry. By combining several individual SVMs, the proposed method achieves better performance than the SVME of all base SVMs.
基金This work is partially supported by the National Key Research and Development Program of China under Grant No. 2016YFB1000205, the State Key Program of National Natural Science Foundation of China under Grant No. 61432002, the National Natural Science Foundation of China-Guangdong Joint Fund under Grant No. U1701263, the National Natural Science Foundation of China under Grant Nos. 61702365, 61672379, and 61772112, the Natural Science Foundation of Tianjin under Grant Nos. 17JCQNJC00700 and 17JCYBJC15500, and the Special Program of Artificial Intelligence of Tianjin Municipal Science and Technology Commission under Grant No. 17ZXRGGX00150.
文摘With the multi-tier pricing scheme provided by most of the cloud service providers （CSPs）, the cloud userstypically select a high enough transmission service level to ensure the quality of service （QoS）, due to the severe penalty ofmissing the transmission deadline. This leads to the so-called over-provisioning problem, which increases the transmissioncost of the cloud user. Given the fact that cloud users may not be aware of their traffic demand before accessing the network,the over-provisioning problem becomes more serious. In this paper, we investigate how to reduce the transmission cost fromthe perspective of cloud users, especially when they are not aware of their traffic demand before the transmission deadline.The key idea is to split a long-term transmission request into several short ones. By selecting the most suitable transmissionservice level for each short-term request, a cost-efiqcient inter-datacenter transmission service level selection framework isobtained. We further formulate the transmission service level selection problem as a linear programming problem andresolve it in an on-line style with Lyapunov optimization. We evaluate the proposed approach with real traffic data. Theexperimental results show that our method can reduce the transmission cost by up to 65.04%.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.52173023,21704082 and 21875182)Key Scientific and Technological Innovation Team Project of Shaanxi Province(No.2020TD-002)Natural Science Foundation of Shaanxi Province(No.2020JQ-015),and 111 project 2.0(No.BP2018008).The X-ray data of this research was undertaken on the SAXS/WAXS beamline at the Australian Synchrotron,part of ANSTO.
文摘The aligned molecular packing structure is vital to the anisotropic charge transport in conjugated polymer and small molecule thin films.However,how this molecular packing motif influences the photoelectric conversion process at the donor/acceptor heterojunction is still mysterious.Herein,we employed a PM6/Y6 bilayer model to investigate the long-range alignment of molecular packing induced photoelectric conversion process.Both PM6 and Y6 layers were properly controlled to exhibit the uniaxially oriented molecular packing compared to their as-cast counterparts,as revealed by the polarized absorption spectra and transmission electron microscopy.After analyzing the photovoltaic performance of bilayer devices,the smaller energy loss,lower energetic disorder,and longer charge carrier lifetime can be observed in the bilayer devices with aligned Y6 molecules,which contribute to a higher power conversion efficiency(PCE)than the as-cast devices.While the molecular packing structure of PM6 layer exhibited negligible influence on the device performance,probably resulting from the intrinsic semicrystalline nature of PM6 molecules.Our results indicate that the alignment of small molecular acceptor at the donor/acceptor interfaces should be a powerful strategy to facilitate the photoelectric conversion process,which will definitely pave the way to highly efficient bulk heterojunction photovoltaic device.
基金This work was supported in parts by the National Key R&D Program of China(2017YFC1001402 to H.W.,2018YFC1004102 to J.L.)National Natural Science Foundation of China(81490744 to H.W.,31600945 to J.L.and 31701016 to J.W.)+2 种基金Fujian Natural Science Foundation(2017J01071 to J.L.)the Fundamental Research Funds for the Central Universities(20720180041 J.L.)Foundation from Key Laboratory of Reproduction Regulation of NPFPC(2017KF01 to J.L.).The funders had no role in study design,data collection,and analysis,decision to publish,or preparation of the manuscript
文摘Proper development of the human placenta is of vital importance for a successful pregnancy,and a series of pregnancy complications are considered originating from dysfunctional placentas.Like other organ system development,placentation requires large numbers of co-regulators,while the underlying molecular mechanisms orchestrating the placental formation and function are poorly understood.Although we have made many signs of progress in understanding the placental architectures and developments using mouse models,the species-specific differences impede our progress due to the lack of appropriate model systems.In the past few years,major progress has been made by the establishment of novel in-vitro self-renewing stem cell models,as well as identifying the full picture of the cellular organization of the maternal and fetal interface.Providing the tools for the investigation of placentation and reproductive-related regulation mechanism.In this review,we focus on the detailed progress of the human trophoblast stem cells culturing system,and the cellular and molecular terrain at the maternal-fetal interface,respectively,thus providing new insights into placental development.
