AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The H...AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX（1×10^-6mol/L） group,HA1077（3×10^-5mol/L）group,and DEX（1×10^-6mol/L） and HA1077（3×10^-5mol/L）group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS： In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks（CLANs）,which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION： Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.展开更多
This paper presents an adaptive terminal sliding mode control(ATSMC) method for automatic train operation. The criterion for the design is keeping high-precision tracking with relatively less adjustment of the control...This paper presents an adaptive terminal sliding mode control(ATSMC) method for automatic train operation. The criterion for the design is keeping high-precision tracking with relatively less adjustment of the control input. The ATSMC structure is designed by considering the nonlinear characteristics of the dynamic model and the parametric uncertainties of the train operation in real time. A nonsingular terminal sliding mode control is employed to make the system quickly reach a stable state within a finite time, which makes the control input less adjust to guarantee the riding comfort. An adaptive mechanism is used to estimate controller parameters to get rid of the need of the prior knowledge about the bounds of system uncertainty. Simulations are presented to demonstrate the effectiveness of the proposed controller, which has robust performance to deal with the external disturbance and system parametric uncertainties. Thereby, the system guarantees the train operation to be accurate and comfortable.展开更多
基金Supported by the Natural Science Foundation of China (No.81300768 No.81371048+4 种基金 No.81670853)Science and Technology Department of Sichuan Province of China (No.2015HH0031)Health and Family Planning Commission of Sichuan Province of China (No.100539 No. 090505 No.090514)
文摘AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX（1×10^-6mol/L） group,HA1077（3×10^-5mol/L）group,and DEX（1×10^-6mol/L） and HA1077（3×10^-5mol/L）group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS： In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks（CLANs）,which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION： Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.
基金supported by National Natural Science Foundation of China and High Speed Railway Union Foundation of China(No.U11344205)
文摘This paper presents an adaptive terminal sliding mode control(ATSMC) method for automatic train operation. The criterion for the design is keeping high-precision tracking with relatively less adjustment of the control input. The ATSMC structure is designed by considering the nonlinear characteristics of the dynamic model and the parametric uncertainties of the train operation in real time. A nonsingular terminal sliding mode control is employed to make the system quickly reach a stable state within a finite time, which makes the control input less adjust to guarantee the riding comfort. An adaptive mechanism is used to estimate controller parameters to get rid of the need of the prior knowledge about the bounds of system uncertainty. Simulations are presented to demonstrate the effectiveness of the proposed controller, which has robust performance to deal with the external disturbance and system parametric uncertainties. Thereby, the system guarantees the train operation to be accurate and comfortable.
