Targeted metabolomics study of fatty-acid metabolism in lean metabolic-associated fatty liver disease patients

BACKGROUND The annual incidence of metabolic-associated fatty liver disease(MAFLD)in China has been increasing and is often overlooked owing to its insidious charac-teristics.Approximately 50%of the patients have a normal weight or are not obese.They are said to have lean-type MAFLD,and few studies of such patients are available.Because MAFLD is associated with abnormal lipid metabolism,lipid-targeted metabolomics was used in this study to provide experimental evidence for early diagnosis and pathogenesis.MAFLD and analyze metabolic pathways.UPLC-Q-Orbitrap/MS content determination was used to determine serum palmitic acid(PA),oleic acid(OA),linoleic acid(LA),and arachidonic acid(AA)levels in lean-type MAFLD patients.RESULTS Urea nitrogen and uric acid levels were higher in lean-type MAFLD patients than in healthy individuals(P<0.05).Alanine transaminase and cholinesterase levels were higher in lean-type MAFLD patients than in healthy indi-viduals(P<0.01).The expression of high-density lipoprotein and apolipoprotein A-1 were lower in lean-type MAFLD patients than in healthy individuals(P<0.05)and the expression of triglycerides and fasting blood glucose were increased(P<0.01).A total of 65 biomarkers that affected the synthesis and metabolism of fatty acids were found with P<0.05 and variable importance in projection>1.The levels of PA,OA,LA,and AA were significantly increased compared with healthy individuals.CONCLUSION The metabolic profiles of lean-type MAFLD patients and healthy participants differed significantly,yielding 65 identified biomarkers.PA,OA,LA,and AA exhibited the most significant changes,offering valuable clinical guidance for prevention and treatment of lean-type MAFLD.

Psychological Status and Warfarin Therapy in Patients after Valve Replacement during the COVID-19 Pandemic:A Cross-sectional Study

Objective The standardization of warfarin anticoagulant therapy is the key to lifelong treatment for patients after heart valve replacement.The present study explored the possible risk factors for anxiety and depression during the coronavirus disease 2019(COVID-19)pandemic and analyzed the influence of psychological state on medication safety.Methods Eligible patients received a web-based questionnaire survey via the Wenjuanxing platform during outpatient visits.Depression was evaluated by the Self-Rating Depression Scale(SDS).Anxiety was evaluated by the Self-Rating Anxiety Scale(SAS).Medication adherence was evaluated by the Morisky scale.Results A total of 309 patients(aged 52.2±11.4 years)were included in the present study.The SDS score of all included patients was 36.9±9.4 points,of which 11(3.6%)patients were diagnosed as having depression.The SAS score of all included patients was 43.1±9.3 points,of which 71(23%)patients were diagnosed as having anxiety.Seven patients(2.3%)had both anxiety and depression.Logistic regression analysis revealed that only monthly income was an independent influencing factor for depression.Regarding anxiety,patients who underwent repeated operations had a 2.264-fold greater risk,and patients who received combination medication had a 2.140-fold greater risk.More bleeding events and coagulation disorders could be observed in patients with anxiety,depression or both.When anxiety occurred,patients showed worse medication adherence.However,depression had no significant effect on medication adherence.Conclusion During the COVID-19 pandemic,the detection rate of mental illnesses such as anxiety and depression was high,which seriously affected the medication safety of warfarin.Analysis of its influencing factors will provide a reference for further standardized regulation of warfarin anticoagulant therapy after valve replacement.

Regional volume changes of the brain in migraine chronification

The pathophysiology of migraine is complex.Neuroimaging studies reveal functional and structural changes in the brains of migraine patients.We sought to explore regional volume differences in intracranial structures in patients with episodic and chronic migraine.Sixteen episodic migraine patients,16 chronic migraine patients,and 24 normal controls were recruited and underwent 3.0 T MRI scanning.The volumes of 142 brain regions were calculated by an automatic volumetric algorithm and compared with clinical variables.Results demonstrated that the volumes of specific regions in the frontal and occipital lobes,and the right putamen,were increased and the volume of the fourth ventricle was decreased in the episodic migraine patients compared with controls.The volumes of the left basal forebrain,optic chiasm,and,the fourth ventricle were decreased in the chronic migraine patients,while the occipital cortex and the right putamen were larger.Compared to episodic migraine patiants,chronic migraine patients displayed larger left thalamus and smaller frontal regions.Correlation analysis showed that headache frequency was negatively correlated with the volume of the right frontal pole,right lateral orbital gyrus,and medial frontal lobes and positively correlated with the volume of the left thalamus.The sleep disturbance score was negatively correlated with the volume of the left basal forebrain.This suggests that migraine patients have structural changes in regions associated with pain processing and modulation,affective and cognitive processing,and visual perception.The remodeling of selective intracranial structures may be involved in migraine attacks.This study was approved by the Ethics Committee of Chinese PLA General Hospital(approval No.S2018-027-02)on May 31,2018.

The first data release(DR1) of the LAMOST regular survey

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

The local properties of supernova explosions and their host galaxies

We aim to understand the properties at the locations of supernova(SN) explosions in their host galaxies and compare with the global properties of these host galaxies. We use the integral field spectrograph(IFS) of Mapping Nearby Galaxies at Apache Point Observatory(MaNGA) to generate 2 D maps of the parameter properties for 11 SN host galaxies. The sample galaxies are analyzed one by one in detail in terms of their properties of velocity field, star formation rate, oxygen abundance, stellar mass, etc.This sample of SN host galaxies has redshifts around z^0.03, which is higher than those of previous related works. The higher redshift distribution allows us to obtain the properties of more distant SN host galaxies. Metallicity(gas-phase oxygen abundance) estimated from integrated spectra can represent the local metallicity at SN explosion sites with small bias. All the host galaxies in our sample are metal-rich galaxies(12+log(O/H)> 8.5) except for NGC 6387, which means SNe may be more inclined to explode in metallicity-rich galaxies. There is a positive relation between global gas-phase oxygen abundance and the stellar mass of host galaxies. We also try to compare the differences of the host galaxies between SNe Ia and SNe II. In our sample, both SNe Ia and SNe II can explode in normal galaxies, but SNe II can also explode in an interacting or a merging system, in which star formation is occurring in the galaxy.

Data release of the LAMOST pilot survey

This paper describes the data release of the LAMOST pilot survey, which includes data reduction, calibration, spectral analysis, data products and data access. The accuracy of the released data and the information about the FITS headers of spectra are also introduced. The released data set includes 319 000 spectra and a catalog of these objects.

A nuclear-localized cysteine desulfhydrase plays a role in fruit ripening in tomato

Hydrogen sulfide(H_(2)S)is a gaseous signaling molecule that plays multiple roles in plant development.However,whether endogenous H_(2)S plays a role in fruit ripening in tomato is still unknown.In this study,we show that the H_(2)S-producing enzyme L-cysteine desulfhydrase SILCD1 localizes to the nucleus.By constructing mutated forms of SILCD1,we show that the amino acid residue K24 of SILCD1 is the key amino acid that determines nuclear localization.Silencing of SILCD1 by TRV-SILCD1 accelerated fruit ripening and reduced H_(2)S production compared with the control.A SILCD1 gene-edited mutant obtained through CRISPR/Cas9 modification displayed a slightly dwarfed phenotype and accelerated fruit ripening.This mutant also showed increased cysteine content and produced less H_(2)S,suggesting a role of SILCD1 in H_(2)S generation.Chlorophyll degradation and carotenoid accumulation were enhanced in the SILCD1 mutant.Other ripening-related genes that play roles in chlorophyll degradation,carotenoid biosynthesis,cell wall degradation,ethylene biosynthesis,and the ethylene signaling pathway were enhanced at the transcriptional level in the lcd1 mutant.Total RNA was sequenced from unripe tomato fruit treated with exogenous H_(2)S,and transcriptome analysis showed that ripening-related gene expression was suppressed.Based on the results for a SILCD1 gene-edited mutant and exogenous H_(2)S application,we propose that the nuclear-localized cysteine desulfhydrase SILCD1 is required for endogenous H_(2)S generation and participates in the regulation of tomato fruit ripening.

Autosomal recessive 333 base pair interleukin 10 receptor alpha subunit deletion in very early-onset inflammatory bowel disease

BACKGROUND Interleukin 10 receptor alpha subunit(IL10RA)dysfunction is the main cause of very early-onset inflammatory bowel disease(VEO-IBD)in East Asians.AIM To identify disease-causing gene mutations in four patients with VEO-IBD and verify functional changes related to the disease-causing mutations.METHODS From May 2016 to September 2020,four young patients with clinically diagnosed VEO-IBD were recruited.Before hospitalization,using targeted gene panel sequencing and trio-whole-exome sequencing(WES),three patients were found to harbor a IL10RA mutation(c.301C>T,p.R101W in one patient;c.537G>A,p.T179T in two patients),but WES results of the fourth patient were not conclusive.We performed whole-genome sequencing(WGS)on patients A and B and reanalyzed the data from patients C and D.Peripheral blood mononuclear cells(PBMCs)from patient D were isolated and stimulated with lipopolysaccharide(LPS),interleukin 10(IL-10),and LPS+IL-10.Serum IL-10 levels in four patients and tumor necrosis factor-α(TNF-α)in the cell supernatant were determined by enzyme-linked immunosorbent assay.Phosphorylation of signal transducer and activator of transcription 3(STAT3)at Tyr705 and Ser727 in PBMCs was determined by western blot analysis.RESULTS The four children in our study consisted of two males and two females.The age at disease onset ranged from 18 d to 9 mo.After hospitalization,a novel 333-bp deletion encompassing exon 1 of IL10RA was found in patients A and B using WGS and was found in patients C and D after reanalysis of their WES data.Patient D was homozygous for the 333 bp deletion.All four patients had elevated serum IL-10 levels.In vitro,IL-10-stimulated PBMCs from patient D failed to induce STAT3 phosphorylation at Tyr705 and only minimally suppressed TNF-αproduction induced by LPS.Phosphorylation at Ser727 in PBMCs was not affected by LPS or LPS+IL-10 in both healthy subjects and in patient D.CONCLUSION WGS revealed a novel 333-bp deletion of IL10RA in four patients with VEO-IBD,whereas the WES results were inconclusive.

M Dwarf catalog of LAMOST general survey data release one

We present a spectroscopic catalog of 93 619 M dwarfs from the first data release of the Large Sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST）general survey. During sample selection, M giant contamination was eliminated using2 MASS photometry and Ca H/TiO molecular indices. For each spectrum, the spectral subtype and values are provided including radial velocity, Hα equivalent width, a series of prominent molecular band indices, and the metal–sensitive parameter ζ, as well as distances and the space motions for high S/N objects. In addition, Hα emission lines are measured to examine the magnetic activity properties of M dwarfs and 7179 active ones are found. In particular, a subsample with significant variation in magnetic activity is revealed through observations from different epochs. Finally, statistical analysis for this sample is performed, including the metallicity classification, the distribution of molecular band indices and their errors.

A sample of galaxy pairs identified from the LAMOST spectral survey and the Sloan Digital Sky Survey

A small fraction （〈 10%） of the SDSS main galaxy （MG） sample has not been targeted with spectroscopy due to the effect of fiber collisions. These galaxies have been compiled into the input catalog of the LAMOST ExtraGAlactic Surveys and named the complementary galaxy sample. In this paper, we introduce this project and status of the spectroscopies associated with the complementary galaxies in the first two years of the LAMOST spectral survey （till Sep. of 2014）. Moreover, we present a sample of 1102 galaxy pairs identified from the LAMOST complementary galaxies and SDSS MGs, which are defined as two members that have a projected distance smaller than 100 h^-01kpc and a recessional velocity difference smaller than 500 km s-1. Compared with galaxy pairs that are only selected from SDSS, the LAMOST- SDSS pairs have the advantages of not being biased toward large separations and therefore act as a useful supplement in statistical studies of galaxy interaction and galaxy merging.

Multiwavelength study of nearly face-on low surface brightness disk galaxies

We study the ages of a large sample （1802） of nearly face-on disk low surface brightness galaxies （LSBGs） using the evolutionary population synthesis （EPS） model PEGASE with an exponentially decreasing star formation rate to fit their mul- tiwavelength spectral energy distributions （SEDs） from far-ultraviolet （FUV） to nearinfrared （NIR）. The derived ages of LSBGs are 1-5 Gyr for most of the sample no matter if constant or varying dust extinction is adopted, which are similar to most of the previous studies on smaller samples. This means that these LSBGs formed the majority of their stars quite recently. However, a small part of the sample （~2%-3%） has larger ages of 5-8 Gyr, meaning their major star forming process may have occurred earlier. At the same time, a large sample （5886） of high surface brightness galaxies （HSBGs） are selected and studied using the same method for comparisons. The de- rived ages are 1-5 Gyr for most of the sample （97%） as well. These results probably mean that these LSBGs have not much different star formation histories from their HSBGs counterparts. However, we should notice that the HSBGs are generally about 0.2 Gyr younger, which could mean that the HSBGs may have undergone more recent star forming activities than the LSBGs.

Immune Responses to Trichloroethylene and Skin Gene Expression Profiles in Sprague Dawley Rats

Objective To characterize the immune reaction in SD rats exposed to trichloroethylene （TCE） and to identify the gene expression profiles involved in skin after TCE exposure. Methods Fifteen percent of TCE was injected intradermally into the rat back （100 μL/120 g） at intervals of 7 days. Whole blood was collected 24 h after the fifth or seventh intradermic administration of TCE. The percentages of CD4＋ and CD8＋ of T lymphocytes were measured by a flow cytometer. The concentrations of IFN-gamma and 1L-4 in the serum were semi-quantified by ELISA. Total RNAs of skin samples at 3 h or 24 h after the seventh dose of TCE in SD rats were extracted, and gene expression proftles of these tissues were analyszed by rat toxicology U34 array of Affymetrix. Results Obvious decline of CD4＋ in T lymphocytes was observed in the TCE-administer group. No significant concentration differences in IFN-gamma and IL-4 were found between TCE-treated and control rats. Gadd45a and Mel were significantly up regulated in skin tissue 24 h after TCE exposure. The expression regulation of immune response factors was as active as proteins associated with lipid metabolism and synthesis process in these skin samples of SD rats exposed to TCE. Conclusion T-helper type 1 cells mediate immune response can not be elicited in TCE-treated SD rats, but certain immune disorder can be induced.

A search for double-peaked narrow emission line galaxies and AGNs in the LAMOST DR1

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope （LAMOST） has released more than two million spectra, which provide the opportunity to search for double-peaked narrow emission line （NEL） galaxies and active galactic nuclei （AGNs）. The double-peaked narrow-line profiles can be well modeled by two velocity components, respectively blueshifted and redshifted with respect to the sys- temic recession velocity. This paper presents 20 double-peaked NEL galaxies and AGNs found from LAMOST DR1 using a search method based on a multi-Gaussian fit of the narrow emission lines. Among them, ten have already been published by other authors, either listed as genuine double-peaked NEL objects or as asymmetric NEL objects, and the remaining ten are original discoveries. We discuss some pos- sible origins for the double-peaked narrow-line features, such as interaction between jet and narrow line regions, interaction with companion galaxies, and black hole bina- ries. Spatially resolved optical imaging and/or follow-up observations in other spectral bands are needed to further discuss the physical mechanisms at work.

Gaseous versus Stellar Velocity Dispersion in Emission-Line Galaxies

We compare the ionized gas velocity dispersion σgas with the stellar velocity dispersion σ＊ in star-forming galaxies, composite galaxies, Low Ionization Nuclear Emissionline Regions （LINERs） and Seyfert 2s, compiled from a cross-identification of Sloan Digital Sky Survey Fourth Data Release （SDSS DR4） and Point Source Catalogue Redshift Survey （PSCz）. We measure σgas from the FWHMs of emission lines （Hα, [NⅡ]λλ6548, 6583 and [SⅡ]λλ6716, 6731）. A significant correlation between the gas and stellar velocity dispersion exists, despite substantial scatter. The mean value of the gas to stellar velocity dispersion ratio is close to unity. This suggests that gas velocity dispersion can substitute for the stellar velocity dispersion as a tracer of the gravitational potential well for all the four types of galaxies, but the involved uncertainties are different from type to type. We also studied -↑σgas, as a function of the redshift and the axial ratio to test the effects of aperture and galaxy inclination, and found that both effects are weak. Finally we checked the trend of -↑σgas/σ＊ with the infrared luminosity and found no significant correlation.

Nearby M subdwarfs from LAMOST data release 2

We identify 108 M subdwarfs（sd Ms） out of more than two hundred thousand M type spectra from the second data release（DR2） of the LAMOST regular survey. This sample, among which 58 members are identified for the first time, includes 33 extreme subdwarfs（esd Ms） and 11 ultra subdwarfs（usd Ms）.The selection is based on the usual ratio of absorption depth of Ca H2, Ca H3 and TiO 5 band systems.We also emphasize the use of the Ca H1 band. We provide estimates of spectral subtype（SPT）, L′epine metallicity index ζ, effective temperature and [Fe/H]. Both ζ–[Fe/H] and SPT–Teff figures show reasonable consistency; compared to PHOENIX model spectra, average rounded values of [Fe/H] for sd Ms, esd Ms and usd Ms are respectively –0.5, –1 and –1.5. The photometric distances are estimated, indicating that most sources are located within 500 pc of the Sun and 350 pc of the Galactic disk. Velocities and 3D Galactic motions are also briefly discussed. Among the 108 subdwarfs, seven stars appear to be active with a significant Hα emission line. The source LAMOST J104521.52＋482823.3 is a white dwarf- M subdwarf binary, while LAMOST J123045.52＋410943.8, also active, exhibits carbon features in red.

Candidate members of star clusters from LAMOST DR2

In this work, we provide 2189 photometrically- and kinematically-selected candidate members of 24 star clusters from the LAMOST DR2 catalog. We perform two-step membership identification： selection along the stellar track in the colormagnitude diagram, i.e., photometric identification, and selection from the distribution of radial velocities, i.e. the kinematic identification. We find that the radial velocities from the LAMOST data are very helpful in the membership identification. The mean probability of membership is 40% for the sample selected with radial velocity. With these 24 star clusters, we investigate the performance of the radial velocity and metallicity estimated with the LAMOST pipeline. We find that the systematic offsets in radial velocity and metallicity are 0.85 ± 1.26 km s-1and-0.08 ± 0.04 dex, with dispersions of 5.47＋1.16-0.71 km s-1and 0.13＋0.04-0.02 dex, respectively. Finally, we propose that the photometrically-selected candidate members of the clusters covered by the LAMOST footprint should be assigned higher priority so that more candidate stars can be observed.

A sample of E+A galaxy candidates in the Second Data Release of LAMOST Survey

A sample of 70 E＋A galaxies is selected from 37 206 galaxies in the second data release of the Large Sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST）. This sample is selected according to the criteria for E＋A galaxies defined by Goto, and each of these objects is further visually identified. In this sample, most objects are low redshift E＋A galaxies with z 〈 0.25, and are located in an area of the sky with high Galactic latitude and magnitude from 14 to 18 mag in the g, r and i bands. A stellar population analysis of the whole sample indicates that the E＋A galaxies are characterized by both young and old stellar populations（SPs）, and the metalrich SPs have relatively higher contributions than the metal-poor ones. Additionally, a morphological classification of these objects is performed based on images taken from the Sloan Digital Sky Survey.

Elevated nitrogen and co-evolution history with competitors shape the invasion process of Galinsoga quadriradiata

Invasive plants usually experience population differentiation as they expand from their initial invasive range to the edge.Moreover,invasive plants usually encounter competitors which shared different co-evolutionary histories with them.These factors may lead to varying responses of invasive plant populations to elevated nitrogen deposition during expansion.However,this issue has received limited attention in prior research.To address these challenges,we conducted a greenhouse experiment to investigate how population differentiation of Galinsoga quadriradiata interacts with the presence of various competitors in response to increased nitrogen deposition.Competitor types(new or old that shared short or long co-evolutionary history with the invader,respectively)were set to compete with the invasive central and edge populations under different nitrogen addition treatments.Individuals from the central population of G.quadriradiata,originating from the initial invasion range,showed greater total mass,reproduction and interspecific competitiveness compared with the edge population.Nitrogen addition improved growth and reproductive performance in both populations,and the central population had a stronger response compared with the edge population.The performance of G.quadriradiata was inhibited more effectively by old competitors than new competitors.Our results indicate that population differentiation occurs in terms of growth and competitiveness during the range expansion of G.quadriradiata,with the central population exhibiting superior performance.Co-evolutionary history with competitors is considered unfavorable for invasive plants in this study.Our results highlight the combined effects of population differentiation in invasive species and their co-evolution history with competitors in the context of global change factors.

入侵植物粗毛牛膝菊根际土壤和根系真菌群落会沿海拔扩散路径发生变化

与入侵植物相关的真菌群落可能会随着海拔发生变化,这可能会影响入侵植物的表现和入侵力。我们最近的研究表明,入侵植物粗毛牛膝菊(Galinsoga quadriradiata)的根系AMF侵染率随着海拔的升高而下降。然而,尚不清楚这是否与真菌群落沿海拔的变化相关。为此,我们采用高通量测序技术、功能分组和线性分析等方法,研究了秦巴山区的粗毛牛膝菊根际土壤和根系真菌群落组成与结构沿海拔的变化趋势。研究结果表明,粗毛牛膝菊根际土壤和根系真菌群落物种多样性和种类组成随海拔升高发生了显著变化。在根际土壤中真菌群落香农-威纳多样性指数随着海拔升高呈上升趋势,而在根系中则呈下降趋势。同时,在根际土壤中共生真菌的相对丰度随海拔高度没有发生显著变化,而在根系中呈下降趋势;在根际土壤中病原真菌的相对丰度随着海拔呈下降趋势,而在根系中呈上升趋势。这些研究结果暗示,该种在向研究区域高海拔地区入侵时,土壤共生真菌的供给并不会缺乏,但其与这些真菌之间建立并维持共生关系的能力降低了;在向高海拔扩张过程中,该种倾向于在根系中积累更多的病原真菌,而减少对共生真菌的依赖。这些研究结果说明,入侵植物与真菌群落之间的相互作用会沿着海拔梯度发生显著变化,植物地下部分与真菌的相互作用可能是我们理解外来种如何在高海拔环境中入侵成功的关键。

Study progress on the mechanism of acupuncture for primary dysmenorrhea

Through searching the literature on the mechanism of acupuncture for primary dysmenorrhea in the last 15 years,this paper summarizes systematically the current study progress on the analgesic mechanism of acupuncture for primary dysmenorrhea,from the perspectives of animal experiment and human trials.In terms of research methods,the behavioral observation,evaluation of central and peripheral biochemical indexes,and molecular biological techniques were mainly used in the animal experiments;whilst the clinical symptoms and evaluation of peripheral serum biochemical indexes were focused on in human trials.As for the research results,the animal experiments showed that acupuncture may play an analgesic role by regulating endocrine,promoting the release of central and peripheral neurotransmitters,regulating immune function,and relieving uterine smooth muscle spasm.However,the human trials have found that acupuncture can produce analgesic effects by regulating serum prostaglandin and ovarian hormone levels,promoting the release of peripheral β-endorphin,improving the uterine artery flow status,and relieving uterine smooth muscle spasm.Although different research methods were used in animal and human studies,the similar results were obtained in acupuncture regulating endocrine levels,promoting release of peripheral neurotransmitters,and alleviating uterine smooth muscle spasms.In addition,at present stage,animal experiments are more than human trials in numbers,and there are relatively few studies on the central mechanism of acupuncture analgesia in human trials.Therefore,in view of this phenomenon,this paper proposes to use functional magnetic resonance imaging(fMRI) technology to explore the central mechanism of acupuncture analgesia in human body,so as to provide a new idea for further exploring the mechanism of acupuncture analgesia in treating primary dysmenorrhea.