Teaching Reform and Practice of Functional Experiment Based on the Cultivation of Excellent Medical Talents

In order to cultivate excellent clinical medical talents with a solid foundation, strong literacy, refined skills, excellent communication abilities, innovative thinking, and a strong focus on practicality, functional experiments have undergone a series of reforms in areas such as constructing new curriculum systems, improving teaching content, updating teaching equipment, introducing new teaching models, and enhancing teaching evaluation systems.

Research and Exploration of Ideological and Political Education in the Course of Pathophysiology

Course based ideological and political education (CIPE) is an important way to improve the quality of ideological and political work and talent cultivation. This study explores for the first time the implementation of ideological and political education in the teaching of pathophysiology courses, and also analyzes the evaluation of student psychological status and the importance of mental health education in the implementation of IPE courses. A survey was conducted on 211 students at Yangtze University to understand their motivation and behavior towards learning ideological, political, and pathophysiological courses. In addition, a questionnaire survey was used to explore the relationship between pathophysiology and ideological and political courses, as well as the importance of their satisfaction with the implementation of ideological and political courses in pathophysiology and mental health education. The research results indicate that factors such as educational background and gender differences affect the learning of CIPE. Graduate students are more interested in ideological and political courses, while female students find it difficult to study pathophysiology;In addition, the results of one-way ANOVA indicate that the implementation effect of IPE in pathophysiology courses depends on the level of interest in IPE and pathophysiology courses, the level of consideration for the importance of professional courses, the professional gains after studying pathophysiology, and the level of understanding of the relationship between IPE and CIPE. 81.04% of students believe that in the CIPE process, telling stories by teachers themselves is the most popular way of communication and education. This reflects the importance of mental health education from the perspective of CIPE. In addition, this study also indicates that PBL and flipped classroom teaching models are popular teaching models in CIPE. This study is beneficial for promoting the improvement and implementation of CIPE and mental health education in higher education curricula, thus providing valuable insights for educational decision-makers.

Bioinformatics Analysis Revealed Potential Tumor Suppressors (KLF4/CGN), Oncogenes (SHH/LIF) and Biomarkers of Asian Stomach Adenocarcinoma

Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.

Novel Functional Motifs of the Cell Entry Glycoprotein D for Oncolytic Herpes Simplex Viruses

Background: Oncolytic herpes simplex virus (oHSV) have been proved effective and safe to treat tumors. Glycoprotein D (gD) has been engineered for targeting cancer cells and de-targeting normal cells successfully, however, the effectiveness and safety of oHSVs still need to be improved. Method: Here we sequenced the DNA encoding gD of our recently isolated new strain HSV-1-LXMW and compared the gD amino acid sequence with the gDs of other 7 HSV-1 and 3 HSV-2 strains. Results: Phylogenetic analysis revealed that HSV-1-LXMW is evolutionarily close to HSV-1-Patton and -KOS strains. The gD amino acid sequence alignment identified 19 conserved and 8 variable regions. We further predicted 10 new motifs in HSV gD for the first time and identified motif differences in HSV-1 and HSV-2. We summarized the gD-engineered oHSVs and found that some of the newly identified gD motifs are actually functional. Conclusion: Our results shed light on HSV gD biology and provided new directions for future gD functional studies and engineering in order to make better oHSVs.

CBL Significance in Pathophysiology Teaching

Objective: To explore the importance of CBL in pathophysiology teaching. Methods: 218 undergraduate clinical medicine students in 2015 grade were divided into CBL group and control group, and each group had 109 students. The CBL group was taught by CBL, while the control group was taught by routine teaching mode. The teaching effects was evaluated by questionnaire survey and stage test. Results: The CBL group was superior to the control group in learning interest, logical thinking, knowledge system construction, comprehensive use of knowledge to solve problems. Conclusion: CBL teaching enhanced students’ understanding of knowledge significantly, and also enhanced their clinical thinking and problem analysis ability.

A Study on Lung Cancer in Jianghan Plain

This study was to investigate the epidemiology of lung cancer in Jianghan Plain, providing scientific basis for prevention and treatment of lung cancer. Pathology and clinical data of 1290 patients diagnosed lung cancer from the largest hospital in Jianghan Plain were collected and analyzed. Results found that the lung cancer incidence increased gradually from the age of 40 and at the peak during 61 to 70 years old;the male to female ratio was 2.45:1;Smoking patients were 841 cases, accounting for 65.2% of all cases. Smoke index > 400 was 771 cases accounting for 91.7 percent of smokers and smoking index < 400 was 70 cases accounting for 8.3% of smoking patients. 93.6% of the patients were to start smoking at a young age. The rate of patient pathological cytology diagnosed by fiberoptic bronchoscopy was 31.8%, which showed that it was an important diagnostic method for lung cancer. The main reason for smoking is to break up the monotony and although many patients know nicotine, carbon monoxide is the main smoking harmful substances and smoking may cause lung cancer;not many people can take the initiative to quit smoking. It also showed that squamous cell carcinoma was the main type in smoking patients, while adeno-carcinoma in non-smoking patients. The comprehensive epidemiological analysis suggested that smoking is an important factor in causing lung cancer, smoking cessation education and regular medical examinations to strengthen the prevention of lung cancer have a positive meaning to their family members.

The Role, Mechanism and Transcriptional Regulation of LAT in Herpes Simplex Virus Latency and Reactivation

Herpes simplex virus (HSV) infection in the human body can be latent in neurons for long time and be reactivated leading to recurrence at high rate. Currently there is no effective clinical strategy for the prevention and treatment of the disease relapse. HSV LAT gene is expressed in large quantities and lytic genes are turned off leading to HSV latency. Disruption of the gene expression is thought to cause HSV reactivation and disease relapse. To reveal the essence of HSV latency and reactivation, we summarized and innovatively classified the role, mechanism and transcriptional regulation of LAT in HSV latency and reactivation. This review may have important implications for future studies on HSV latency and reactivation, HSV disease prevention and treatment, and safer and more effective oncolytic HSVs (oHSVs).

The Construction and Implementation of a Cancer Precision Nursing Model

This article reviews the precision nursing of cancer patients from three aspects: concept, system construction and clinical application. It is suggested that nurses should correctly understand the connotation of precision nursing, expand the research content, enrich the research methods, actively carry out the targeted cancer precision nursing practice, improve the quality of life of the patients, and then form the shape of the patient. It is a localized nursing care system that can provide the best medical care for every cancer patient.