In coal mining,rock strata are fractured under cyclic loading and unloading to form fracture channels.Fracture channels are the main flow narrows for gas.Therefore,expounding the flow conductivity of fracture channels...In coal mining,rock strata are fractured under cyclic loading and unloading to form fracture channels.Fracture channels are the main flow narrows for gas.Therefore,expounding the flow conductivity of fracture channels in rocks on fluids is significant for gas flow in rock strata.In this regard,graded incremental cyclic loading and unloading experiments were conducted on sandstones with different initial stress levels.Then,the three-dimensional models for fracture channels in sandstones were established.Finally,the fracture channel percentages were used to reflect the flow conductivity of fracture channels.The study revealed how the particle size distribution of fractured sandstone affects the formation and expansion of fracture channels.It was found that a smaller proportion of large blocks and a higher proportion of small blocks after sandstone fails contribute more to the formation of fracture channels.The proportion of fracture channels in fractured rock can indicate the flow conductivity of those channels.When the proportion of fracture channels varies gently,fluids flow evenly through those channels.However,if the proportion of fracture channels varies significantly,it can greatly affect the flow rate of fluids.The research results contribute to revealing the morphological evolution and flow conductivity of fracture channels in sandstone and then provide a theoretical basis for clarifying the gas flow pattern in the rock strata of coal mines.展开更多
Cardiovascular diseases(CVDs)impose a significant burden worldwide.Despite the elucidation of the etiology and underlying molecular mechanisms of cvDs by numerous studies and recent discovery of effective drugs,their ...Cardiovascular diseases(CVDs)impose a significant burden worldwide.Despite the elucidation of the etiology and underlying molecular mechanisms of cvDs by numerous studies and recent discovery of effective drugs,their morbidity,disability,and mortality are still high.Therefore,precise risk stratification and effective targeted therapies for CvDs are warranted.Recent improvements in single-cell RNA sequencing and spatial transcriptomics have improved our understanding of the mechanisms and cells involved in cardiovascular phylogeny and CvDs.Single-cell RNA sequencing can facilitate the study of the human heart at remarkably high res-olution and cellular and molecular heterogeneity.However,this technique does not provide spatial information,which is essential for understanding homeostasis and disease.Spatial tran-scriptomics can elucidate intracellular interactions,transcription factor distribution,cell spatial localization,and molecular profiles of mRNA and identify cell populations causing the disease and their underlying mechanisms,including cell crosstalk.Herein,we introduce the main methods of RNA-seq and spatial transcriptomics analysis and highlight the latest ad-vances in cardiovascular research.We conclude that single-cell RNA sequencing interprets dis-ease progression in multiple dimensions,levels,perspectives,and dynamics by combining spatial and temporal characterization of the clinical phenome with multidisciplinary tech-niques such as spatial transcriptomics.This aligns with the dynamic evolution of CVDs(e.g.,"angina-myocardial infarction-heart failure"in coronary artery disease).The study of pathways for disease onset and mechanisms(e.g.,age,sex,comorbidities)in different patient subgroups should improve disease diagnosis and risk stratification.This can facilitate precise individualized treatment of CVDs.展开更多
Tyrosinase(TYR) is an important polyphenolic oxidase enzyme and usually regards as a biomarker of melano ma cancer.Highly effective tracking TYR activity in vivo will help to study the mechanism of TYR in living organ...Tyrosinase(TYR) is an important polyphenolic oxidase enzyme and usually regards as a biomarker of melano ma cancer.Highly effective tracking TYR activity in vivo will help to study the mechanism of TYR in living organisms and forecasts related diseases.In this study,we present a novel TYR-activatable fluo rescent probe(CHMC-DOPA) for tracking TYR activity in vitro and in vivo.CHMC-DOPA is constructed by incorporating dopamine(DOPA) moiety into a fluorescent chloro-hydroxyl-merocyanine(CHMC)scaffold.Upon exposure to TYR,the dopamine unit in CHMC-DOPA is oxidized to a dopaquinone derivative,and an intramolecular photo-induced electron transfer(PET) process between CHMC fluorophore and o-dopaquinone will take place,the fluorescence of CHMC-DOPA is quenched rapidly.Therefore,the evaluation of TYR activity is established in terms of the relationship between fluorescence quenching efficiency and TYR activity.In our experiments,CHMC-DOPA shows various advantages,such as fast response(8 min),low concentration of TYR activation(0.5 U/mL),good water-solubility,as well as the lowest detection limit(0.003 U/mL) compared with previously reported works.Furthermore,CHMCDO PA also exhibits excellent cell membrane permeability and low cytotoxicity,which is successfully used to monitor endogenous TYR activity in living cancer cells and zebrafish models.CHMC-DOPA performs well,and we anticipate that this newly designed novel platform will provide an alternative for high effective monitoring TYR activity in biosystems.展开更多
In the presence of the inexpensive and non-toxic polymethylhydrosiloxane, the combination of copper(II) acetateand a chiral diphosphine displayed high catalytic efficiency in the asymmetric hydrosilylation of a series...In the presence of the inexpensive and non-toxic polymethylhydrosiloxane, the combination of copper(II) acetateand a chiral diphosphine displayed high catalytic efficiency in the asymmetric hydrosilylation of a series ofaromatic ketones in air atmosphere and at room temperature. (R)-1-Arylethanols were obtained with up to 99%yield and 93% enantiomeric excess. Meanwhile, the electron effect and steric hindrance of substituents on the aromaticring had an interesting influence on both the yields and enantioselectivities. Furthermore, a possible mechanismwas presented to explain the influence of some key factors on the reaction.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(No.52074041)the Chongqing Talent Program(No.cstc2022ycjh-bgzxm0077)the Postgraduate Research and Innovation Foundation of Chongqing,China(No.CYS23060).
文摘In coal mining,rock strata are fractured under cyclic loading and unloading to form fracture channels.Fracture channels are the main flow narrows for gas.Therefore,expounding the flow conductivity of fracture channels in rocks on fluids is significant for gas flow in rock strata.In this regard,graded incremental cyclic loading and unloading experiments were conducted on sandstones with different initial stress levels.Then,the three-dimensional models for fracture channels in sandstones were established.Finally,the fracture channel percentages were used to reflect the flow conductivity of fracture channels.The study revealed how the particle size distribution of fractured sandstone affects the formation and expansion of fracture channels.It was found that a smaller proportion of large blocks and a higher proportion of small blocks after sandstone fails contribute more to the formation of fracture channels.The proportion of fracture channels in fractured rock can indicate the flow conductivity of those channels.When the proportion of fracture channels varies gently,fluids flow evenly through those channels.However,if the proportion of fracture channels varies significantly,it can greatly affect the flow rate of fluids.The research results contribute to revealing the morphological evolution and flow conductivity of fracture channels in sandstone and then provide a theoretical basis for clarifying the gas flow pattern in the rock strata of coal mines.
基金supported by the National Natural Science Foundation of China (No.82204943)the China Postdoctoral Science Foundation (No.2022M710471)+1 种基金the New Teacher Program of Beijing University of Chinese Medicine (2023-JYB-XJSJJ034)the 2023 Graduate independent research Project of Beijing University of Chinese Medicine (90011461220378)awarded.
文摘Cardiovascular diseases(CVDs)impose a significant burden worldwide.Despite the elucidation of the etiology and underlying molecular mechanisms of cvDs by numerous studies and recent discovery of effective drugs,their morbidity,disability,and mortality are still high.Therefore,precise risk stratification and effective targeted therapies for CvDs are warranted.Recent improvements in single-cell RNA sequencing and spatial transcriptomics have improved our understanding of the mechanisms and cells involved in cardiovascular phylogeny and CvDs.Single-cell RNA sequencing can facilitate the study of the human heart at remarkably high res-olution and cellular and molecular heterogeneity.However,this technique does not provide spatial information,which is essential for understanding homeostasis and disease.Spatial tran-scriptomics can elucidate intracellular interactions,transcription factor distribution,cell spatial localization,and molecular profiles of mRNA and identify cell populations causing the disease and their underlying mechanisms,including cell crosstalk.Herein,we introduce the main methods of RNA-seq and spatial transcriptomics analysis and highlight the latest ad-vances in cardiovascular research.We conclude that single-cell RNA sequencing interprets dis-ease progression in multiple dimensions,levels,perspectives,and dynamics by combining spatial and temporal characterization of the clinical phenome with multidisciplinary tech-niques such as spatial transcriptomics.This aligns with the dynamic evolution of CVDs(e.g.,"angina-myocardial infarction-heart failure"in coronary artery disease).The study of pathways for disease onset and mechanisms(e.g.,age,sex,comorbidities)in different patient subgroups should improve disease diagnosis and risk stratification.This can facilitate precise individualized treatment of CVDs.
基金We gratefully acknowledge the financial support by the National Natural Science Foundation of China(Nos.21702053,21901066,21676075,51603064)Natural Science Foundation of Hubei Province(No.2020CFB601)+1 种基金Outstanding Young and Middle-aged Scientific Innovation Team of Colleges and Universities of Hubei Province(No.T201908)partially supported by Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering.
文摘Tyrosinase(TYR) is an important polyphenolic oxidase enzyme and usually regards as a biomarker of melano ma cancer.Highly effective tracking TYR activity in vivo will help to study the mechanism of TYR in living organisms and forecasts related diseases.In this study,we present a novel TYR-activatable fluo rescent probe(CHMC-DOPA) for tracking TYR activity in vitro and in vivo.CHMC-DOPA is constructed by incorporating dopamine(DOPA) moiety into a fluorescent chloro-hydroxyl-merocyanine(CHMC)scaffold.Upon exposure to TYR,the dopamine unit in CHMC-DOPA is oxidized to a dopaquinone derivative,and an intramolecular photo-induced electron transfer(PET) process between CHMC fluorophore and o-dopaquinone will take place,the fluorescence of CHMC-DOPA is quenched rapidly.Therefore,the evaluation of TYR activity is established in terms of the relationship between fluorescence quenching efficiency and TYR activity.In our experiments,CHMC-DOPA shows various advantages,such as fast response(8 min),low concentration of TYR activation(0.5 U/mL),good water-solubility,as well as the lowest detection limit(0.003 U/mL) compared with previously reported works.Furthermore,CHMCDO PA also exhibits excellent cell membrane permeability and low cytotoxicity,which is successfully used to monitor endogenous TYR activity in living cancer cells and zebrafish models.CHMC-DOPA performs well,and we anticipate that this newly designed novel platform will provide an alternative for high effective monitoring TYR activity in biosystems.
基金the National Natural Science Foundation of China(No.21402066)the Open Project of the Key Laboratory of the Chinese Ministry of Education in Resource Chemistry.
文摘In the presence of the inexpensive and non-toxic polymethylhydrosiloxane, the combination of copper(II) acetateand a chiral diphosphine displayed high catalytic efficiency in the asymmetric hydrosilylation of a series ofaromatic ketones in air atmosphere and at room temperature. (R)-1-Arylethanols were obtained with up to 99%yield and 93% enantiomeric excess. Meanwhile, the electron effect and steric hindrance of substituents on the aromaticring had an interesting influence on both the yields and enantioselectivities. Furthermore, a possible mechanismwas presented to explain the influence of some key factors on the reaction.