Association between specific proteins and dimorphic growth of penicillium marneffei

Objective： To investigate the difference of proteome between yeast form and mould form of Penicillium mameffei, and to investigate the association of specific proteins expressed with biochemical properties, susceptibility of antifungal agent with dimorphic growth. Methods： Biochemistry identity plates were used to test the assimilation of carbohydrates and E-test strips were used to detect the minimum inhibitory concentration （MIC） of mould form and yeast form 16 P. mameffei. Surface enhanced laser desorption/ionization （SELDI） mass spectrometry with ProteinChip WCX2 was performed to compare the expressed proteins in yeast form and mould form. Protein profiles were read by PBSⅡ proteinchip reader and the proteome database was analyzed by proteincbip software 3.2.0. Results： Mould form assimilated lactose, melibiose significantly stronger （ P 〈0.01）, while yeast form assimilated sorbinose significantly stronger （ P 〈 0.05）. The mean MIC of fluconazole against mould form increased significantly （ P 〈 0.01 ） compared with yeast form. Seventy-five distinct proteins were found in yeast form and mould form of P. mameffei, in which proteins of 2900Da and 3151Da were specifically expressed in yeast form and other two proteins of 13151Da and 13285Da were specifically expressed in mould form （ P 〈 0.01 ）. Conclusion： The assimilation of carbohydrates and drug susceptibility of P. mameffei may change partly due to the morphogenetic conversion and different texture. Specific proteins may he involved in the regulation, the change of biochemical reaction and drug susceptibility during dimorpbic growth.

胆甾相液晶微球光子禁带的动态调控及其应用

胆甾相液晶微球是空间结构高度对称的三维光子晶体,能够全方位地选择性反射特定波长的圆偏振光,具有无角度依赖性的光子禁带,作为一种新兴的光学材料在全向激光器、反射式显示和微传感器等领域展现出广阔的应用前景。近年来,毛细管微流控技术的蓬勃发展为连续、可控、高通量地制备结构复杂且分子规则取向的单分散胆甾相液晶微球提供了强有力的支持。本综述重点关注利用毛细管微流控技术制备胆甾相液晶微球的相关研究工作,首先分析了毛细管微流控装置在设计微球结构中的决定性作用,阐明了溶液体系的选择与液晶分子取向间的关系以及边界效应对微球尺寸的影响;随后,从胆甾相液晶微球的光学特性切入,介绍了利用温度、溶剂和光等外界刺激调控螺旋结构自组装的原理和策略以及微球之间独有的“光子交叉通讯”现象;最后,总结了现阶段胆甾相液晶微球的潜在应用方向并讨论了该材料体系未来面临的挑战。

基于聚二甲基硅氧烷弹性体的摩擦电纳米发电机的研究进展

随着全球气候变化与环境污染问题日益严峻,可再生能源技术越来越受到关注。摩擦电纳米发电机(TENGs)可以将机械能转为电能,是重要的能量收集技术之一。TENG由于成本低、效率高且输出功率大,被广泛用于微纳电源、自供电传感器、大规模海洋能量收集和高压直接电源等领域。TENG的输出性能主要取决于摩擦电材料,聚二甲基硅氧烷(PDMS)由于具有良好的柔韧性、生物相容性及负极性是优异的负摩擦电材料。文中综述了PDMS作为负摩擦电材料的优势,探讨了PDMS的物理改性或化学改性对TENG性能的影响,并深入分析了PDMS基TENG的应用及存在的问题。

Effects of dense planting patterns on photosynthetic traits of different vertical layers and yield of wheat under different nitrogen rates

A two-year field experiment was conducted to measure the effects of densification methods on photosynthesis and yield of densely planted wheat.Inter-plant and inter-row distances were used to define ratefixed pattern(RR)and row-fixed pattern(RS)density treatments.Meanwhile,four nitrogen(N)rates(0,144,192,and 240 kg N ha-1,termed N0,N144,N192,and N240)were applied with three densities(225,292.5,and 360×10^(4)plants ha^(-1),termed D225,D292.5,and D360).The wheat canopy was clipped into three equal vertical layers(top,middle,and bottom layers),and their chlorophyll density(Ch D)and photosynthetically active radiation interception(FIPAR)were measured.Results showed that the response of Ch D and FIPAR to N rate,density,and pattern varied with different layers.N rate,density,and pattern had significant interaction effects on Ch D.The maximum values of whole-canopy Ch D in the two seasons appeared in N240 combined with D292.5 and D360 under RR,respectively.Across two growing seasons,FIPAR values of RR were higher than those of RS by 29.37%for the top layer and 5.68%for the middle layer,while lower than those of RS by 20.62%for the bottom layer on average.With a low N supply(N0),grain yield was not significantly affected by density for both patterns.At N240,increasing density significantly increased yield under RR,but D360 of RS significantly decreased yield by 3.72%and 9.00%versus D225 in two seasons,respectively.With an appropriate and sufficient N application,RR increased the yield of densely planted wheat more than RS.Additionally,the maximum yield in two seasons appeared in the combination of D360 with N144 or N192 rather than of D225 with N240 under both patterns,suggesting that dense planting combined with an appropriate N-reduction application is feasible to increase photosynthesis capacity and yield.

Exploration of Teaching Reform of TCM Dispensing Technology in Higher Vocational Colleges Based on Traditional Chinese Medicine Skills Competition

This paper aims to thoroughly explore the teaching reform of Traditional Chinese Medicine(TCM)dispensing technology in higher vocational colleges,with a focus on the Traditional Chinese Medicine Skills Competition.By analyzing the current teaching status and main challenges of TCM dispensing technology,and aligning with the requirements of the skills competition,a series of detailed and practical teaching reform measures are proposed.These measures are designed to comprehensively improve the teaching quality of TCM dispensing technology in higher vocational colleges,enhance students’practical skills and professional qualities,and ultimately meet the demand for high-quality,skilled talents in the field of TCM.

免疫检查点抑制剂治疗恶性肿瘤引起的超进展现象分析

免疫检查点抑制剂为部分癌症患者带来显著生存获益,但是部分患者在治疗早期阶段却出现了肿瘤加速生长现象,称为超进展。超进展是一种全新的肿瘤进展方式,预后极差,常伴有患者生存质量的明显下降。超进展的评估大部分以肿瘤生长速率为基础,并使用自然对数进行校正获得。超进展分子机制尚未明确,与肿瘤细胞基因异常表达、肿瘤微环境及淋巴细胞亚型的改变相关。超进展的预测因素尚未清楚,部分临床病理特征、分子标志物和超进展体现出一定的相关性。本文总结了免疫检查点抑制剂引起超进展的定义、评估、发病情况及预测因子,并对发病机制进行初步探讨。

P4S10 modified lithium anode for enhanced performance of lithium–sulfur batteries

To address the corrosion and dendrite issues of lithium metal anodes, a protective layer was ex-situ constructed by P4S10 modification. It was determined by X-ray photoelectron spectroscopy and Raman spectra that the main constituents of the protective layer were P4S10, Li3PS4 and other LixPySztype derivatives. The protective layer was proved to be effective to stabilize the interphase of lithium metal. With the modified Li anodes, symmetric cells could deliver stable Li plating/stripping for 16000 h;Li–S batteries exhibited a specific capacity of 520 m A h g-1 after 200 cycles at 1000 m A g-1 with average Coulombic efficiency of 97.9%. Therefore, introducing LixPySzbased layer to protect Li anode provides a new strategy for the improvement of Li metal batteries.

Non-invasive and low-artifact in vivo brain imaging by using a scanning acoustic-photoacoustic dual mode microscopy

Photoacoustic imaging is a potential candidate for in vivo brain imaging,whereas,its imaging performance could be degraded by inhomogeneous multi-layered media,consisted of scalp and skull.In this work,we propose a low-artifact photoacoustic microscopy(LAPAM)scheme,which combines conventional acoustic-resolution photoacoustic microscopy with scanning acoustic microscopy to suppress the reflection artifacts induced by multi-layers.Based on similar propagation characteristics of photoacoustic signals and ultrasonic echoes,the ultrasonic echoes can be employed as the filters to suppress the reflection artifacts to obtain low-artifact photoacoustic images.Phantom experiment is used to validate the effectiveness of this method.Furthermore,LAPAM is applied for in-vivo imaging mouse brain without removing the scalp and the skull.Experimental results show that the proposed method successfully achieves the low-artifact brain image,which demonstrates the practical applicability of LAPAM.This work might improve the photoacoustic imaging quality in many biomedical applications which involve tissues with complex acoustic properties,such as brain imaging through scalp and skull.

Knowledge of COVID-19 and its prevention among rural residents in Fuqing,China

Objectives:Rural areas are the weakest place for epidemic prevention and control,yet few studies have specifically conducted surveys in rural areas.The purpose of this study is to assess rural residents’knowledge of the COVID-19 and its prevention in China.Methods:A cross-sectional survey study containing 1,426 samples was conducted in Fuqing,China to assess rural residents’knowledge of the COVID-19 and its prevention.Logistic regression was used to identify potential differences in participants’knowledge of COVID-19 and its prevention and control in different population subgroups.Results:The mean and median of residents’knowledge of COVID-19 was 5.53 and 6 points,respectively.The mean and median of residents’knowledge related to self-protection against COVID-19 was 10.34 and 11 points,respectively.Older adults(AOR45e59=2.26,95%CI 1.20 to 4.27;AOR60-69=3.13,95%CI 1.63 to 5.98;AOR70=4.68,95%CI 2.35 to 9.33)were more deficient in knowledge of COVID-19.Those who were better educated and with a higher average annual household income were less likely to be deficient in the knowledge of COVID-19 and knowledge of self-protection against COVID-19.Moreover,those who were single,divorced,or widowed(AOR=1.67,95%CI 1.13 to 2.46)were more deficient in the knowledge of self-protection against COVID-19.Conclusions:Our study suggests that when facing a pandemic like COVID-19,the key is to inform the public to understand simple and effective self-protection measures.Therefore,we call on the govern-ments to give priority to publicity and education on self-prevention measures for the targeted pop-ulations and regions.This is most evident among the vulnerable groups like those who were unmarried,elderly,and those with low education or low income.Similar suggestions may be of use in other countries as well.

Survey of lightweighting methods of huge 3D models for online Web3D visualization

Background With the rapid development of Web3D technologies, the online Web3D visualization, particularly for complex models or scenes, has been in a great demand. Owing to the major conflict between the Web3D system load and resource consumption in the processing of these huge models, the huge 3D model lightweighting methods for online Web3D visualization are reviewed in this paper. Methods By observing the geometry redundancy introduced by man-made operations in the modeling procedure, several categories of light-weighting related work that aim at reducing the amount of data and resource consumption are elaborated for Web3D visualization. Results By comparing perspectives, the characteristics of each method are summarized, and among the reviewed methods, the geometric redundancy removal that achieves the lightweight goal by detecting and removing the repeated components is an appropriate method for current online Web3D visualization. Meanwhile, the learning algorithm, still in improvement period at present, is our expected future research topic. Conclusions Various aspects should be considered in an efficient lightweight method for online Web3D visualization, such as characteristics of original data, combination or extension of existing methods, scheduling strategy, cache man-agement, and rendering mechanism. Meanwhile, innovation methods, particularly the learning algorithm, are worth exploring.

Controlled Synthesis and Characterization of Nobel Metal Nanoparticles

In this work, monodispersed, well-shaped platinum (3.2 - 6.4 nm), rhodium (2.4 - 5.1 nm), palladium (3.2 - 5.3 nm) nanoparticles capped with poly(vinylpyrrolidone) were synthesized by a polyol reduction method in an ethylene glycol solution at temperature of 190℃. The influences of synthetic parameters on the size and morphology of the noble metal nanoparticles have been systematically investigated. The noble metal nanoparticles were characterized by means of UV-vis, laser scattering particle size distribution analysis (LSPSDA) and transmission electron microscopy (TEM). The experimental results showed that the particle size of metals nanoparticles, the morphology of which was spherical, increased with the raise of metal precursor concentration as well as the amount of PVP. The optimal molar ratio of PVP/metal and metal precursor concentration for the fabrication of Pt, Rh, and Pd nanoparticles with uniform distribution were 10 and 0.1 mM, respectively. The morphologies of the Rh nanoparticles with the size of 5.1 nm were polygons, including hexagons, pentagons, and triangles.

Theoretical study of the radiative decay processes in H^+(D^+,T^+)–Be collisions

The potential energy curves of X~1Σ~+, A~1Σ~+, C~1Σ~+, and B~1Π are calculated with high-level MRDCI method, and the calculated spectroscopic constants of those states are in good agreement with most recent experimental data. On the basis of high precision PECs, the radiative processes of H~++ Be collisions are studied by using the fully quantum, optical potential and semiclassical methods in the energy ranges of 10-8eV/u–0.1 eV/u, and the radiative decay, the radiative charge transfer,and the radiative association cross-sections are computed. It is found that the radiative association process is dominant in the energy region of 10-8eV/u–0.02 eV/u, while radiative charge transfer becomes important at higher energies. Rich resonance structures are present in the radiative association and charge transfer cross-sections in the whole energy region considered, which result from the interaction between the quasi-bound rovibrational(J, v) states in the entrance channel with the final continuum state. Significant isotope effects have been found in the radiative decay processes of H~++ Be collisions.

Rapid Detection of β-lactam Antibiotic Residue in Milk Using Biolayer Interferometry

[ Objective] This study aimed to establish a high-sensitivity method for rapid detection of^-lactam antibiotic residue in milk. [Method] Based on bio- layer interferometry technology, ampicillin-BSA conjugate was fixed on the bottom of APS fiber optic biosensor probe through hydrophobic interaction and bound to 40 mn colloidal gold-labeled/3-1actam antibiotic receptor, to detect β-lactam antibiotics in milk. [ Result] The sensitivity of colloidal gold-labeled BLI method was twice as high as that of immunechromatographic test strip in detection of β-1actam antibiotic residue in milk. Colloidal gold-labeled BLI method exhibited good speci- ficity and had no cross-reaction with 1 000 ng/ml aflatoxin M1, gentamicin, kanamycin, streptomycin, tylosin, chloromycetin and melamine. [Condusion] The colloidal gold-labeled BLI method is not suitable for quantitative detection in actual production due to its small quantitative range in detection of β-lactam antibiot- ics, but it is a simple and rapid qualitative detection method that can be used in rapid detection of β-1actam antibiotic residue in milk.

The Evolutive Law concerning Relationship between Economic Growth and Farmers' Income Increase in Xinjiang since the Reform and Opening-up:An Empirical Analysis of the VEC Model

We select the per capita gross domestic product and rural residents' per capita net income in Xinjiang as the indicator variables to measure economic growth and farmers' income increase in Xinjiang, respectively. We establish the cointegration equation, the vector error correction (VEC) model, and use the impulse response function to conduct empirical analysis of the evolutive law concerning relationship between economic growth and farmers' income increase in Xinjiang during the period 1978-2010. The results show that there is a cointegration relationship between economic growth in Xinjiang and farmers' income increase; the former plays an important role in promoting the latter, following the evolutive law "first intensify, then abate, intensify again, and finally become stable". We put the following policy recommendations: providing preferential policies for farmers, scientifically and rationally guiding the transfer of rural labor; improving the function of agriculture, increasing the proportion of non-farm income; gradually bridging the widening income gap between urban and rural areas, coordinating urban and rural development.

Non-viral targeted delivery system mediates transfection of thymidine kinase gene of herpes simplex virus into ovarian cancer cells:a comparison between one time and continuous mediation

Objective： To compare the transferring efficiency and killing effects of one time and continuous mediation with GE7, a non-viral targeted delivery system, in transfection of thymidine kinase gene of herpes simplex virus （HSV-tk） into ovarian cancer cells. Methods： GE7 was used to prepare recombinants with β-galactosidase （β-gal） and HSVI-tk; the recombinants were then used to transfect human ovarian cancer line CaOV3 once and continuously. β-gal staining was used to compare the efficiencies of one time and continuous mediation with GE7 system. Ganciclovior （GCV） was introduced into HSVI-tk transfected ovarian cells. Through drawing the cell growth curve and flow cytometry, the killing effects of GCV on once and continuously GE7/HSVI-tk transfected cells were observed. Results： We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%, respectively. When 1 μg/mL GCV was used to treat ovarian cell transfected with HSVI-tk gene, growth inhibiting rates of ovarian cells of one time and continuous transferring were 82% and 90%, respectively; their apoptosis indices were 15 and 30, respectively. Under same GCV concentration, continuous mediation of GE7/pCMV-tk transfection into ovarian cancer cells had more significant inhibitory effect than one time mediation （P 〈 0.05）. Conclusion： Compared with one time mediation, continuous mediation of transfection with GE7 gene delivery system has higher efficiency. Continuous mediation of GE7/HSVI-tk/GCV therapeutic gene system has more powerful killing effect.

Value Distribution of the <i>k</i>th Derivatives of Meromorphic Functions

In the paper, we take up a new method to prove a result of value distribution of meromorphic functions: let f be a meromorphic function in , and let , where P is a polynomial. Suppose that all zeros of f have multiplicity at least , except possibly finite many, and as . Then has infinitely many zeros.

微乳液法调控纳米Cu-MOF形貌的实验教学案例

通过微乳液法调控Cu-MOF的形貌,对其制备条件进行优化,得到各种不同形貌的Cu-MOF材料。为了开发材料化学实验与时俱进的教学案例,探究环己烷的用量、反应时间、反应温度等条件对Cu-MOF形貌的影响,从而优化实验细节以满足实验教学案例的要求,使得到的Cu-MOF材料具有重复性好、成本低廉、可行性高的优点。通过本实验教学案例,能够使学生掌握调控纳米MOFs形貌的技能。

Cryogenic System for PKU-FEL

PKU-FEL based on superconducting (SC) accelerator facility is under construction at PekingUniversity. It will run in IR (5 - 10 μm) and THz (100 - 3000 μm) region as an ideal experimental FEL platform for universities. The SC accelerator facility is composed of a DC-SC injector and a 1.3-GHz 2 × 9-cell SC accelerator. In order to better the performance, the injector and the accelerator are bath-cooled by 2 K super fluid helium in cryostats. A 2 K cryogenic system has been designed, constructed and assembled.

Clinical applications of paraumbilical perforator flaps in multiple angiosomes for the reconstruction of the upper limbs

Background:Repair of extensive deep wounds in the forelimb remains challenging for surgeons.The objective of this study was to evaluate the surgical technique and clinical significance of multiple-territory paraumbilical perforator(PUP)flaps in patients with massive soft tissue defects in the upper limbs.Methods:Between January 2017 and September 2021,16 patients(6 women and 10 men)aged 24–54 years(average,41.4 years)who were hospitalized at the First Affiliated Hospital of Soochow University and the North District of the Suzhou Municipal Hospital were investigated.Their injuries included damage to the fingers,dorsal skin of the hands,wrist,or forearm.Their tendons or bones were exposed after debridement.In some patients,multiple-territory PUP flaps that encompassed adjacent angiosomes were transplanted to cover the soft tissue defects.Results:All flaps survived and healed well.After a follow-up of 2–54 months,all patients recovered satisfactorily in terms of characteristic and functional review.Conclusions:The application of PUP flaps,especially those encompassing multiple angiosomes(multiple-territory PUP flaps),can be an optimal reconstruction method for repairing massive soft tissue defects in the forelimb.

makeTwin:A reference architecture for digital twin software platform

Digital twin is currently undergoing a significant transformation from the conceptual and theoretical research phase to the implementation and application phase.However,a universally adaptable research and development platform for digital twin is critically needed to meet the development requirements.Specifically,a publicly accessible simulation,testing,and validation platform which can support digital twin model building,data processing,algorithm design,configuration,etc.,is urgently required for researchers.Furthermore,for developers from the industry,a lowcode development platform that can offer a customizable suite of functions such as model creation,data management,protocol configuration,and visualization is urgently needed.Meanwhile,for enterprise users,there is a lack of an application management platform that can be configured and migrated for various application scenarios,functions,and modes.Therefore,based on the system research of digital twin theories and key technologies by the authors(such as the five-dimension digital twin model,digital twin modeling and digital twin data theory,digital twin standards,and so on),a digital twin software platform reference architecture,namely make Twin,is proposed and designed,as well as its ten core functions.The workflow of the make Twin and the interaction mechanisms among its core functions are described.Finally,a digital twin application system for a chemical fiber textile shop floor(CFTS)which was developed according to make Twin,is introduced,which validates the proposed reference architecture.