Midbrain dopaminergic neurons play an important role in the etiology of neurodevelopmental and neurodegenerative diseases.They also represent a potential source of transplanted cells for therapeutic applications.In vi...Midbrain dopaminergic neurons play an important role in the etiology of neurodevelopmental and neurodegenerative diseases.They also represent a potential source of transplanted cells for therapeutic applications.In vitro differentiation of functional midbrain dopaminergic neurons provides an accessible platform to study midbrain neuronal dysfunction and can be used to examine obstacles to dopaminergic neuronal development.Emerging evidence and impressive advances in human induced pluripotent stem cells,with tuned neural induction and differentiation protocols,makes the production of induced pluripotent stem cell-derived dopaminergic neurons feasible.Using SB431542 and dorsomorphin dual inhibitor in an induced pluripotent stem cell-derived neural induction protocol,we obtained multiple subtypes of neurons,including 20%tyrosine hydroxylase-positive dopaminergic neurons.To obtain more dopaminergic neurons,we next added sonic hedgehog(SHH)and fibroblast growth factor 8(FGF8)on day 8 of induction.This increased the proportion of dopaminergic neurons,up to 75%tyrosine hydroxylase-positive neurons,with 15%tyrosine hydroxylase and forkhead box protein A2(FOXA2)co-expressing neurons.We further optimized the induction protocol by applying the small molecule inhibitor,CHIR99021(CHIR).This helped facilitate the generation of midbrain dopaminergic neurons,and we obtained 31-74%midbrain dopaminergic neurons based on tyrosine hydroxylase and FOXA2 staining.Thus,we have established three induction protocols for dopaminergic neurons.Based on tyrosine hydroxylase and FOXA2 immunostaining analysis,the CHIR,SHH,and FGF8 combined protocol produces a much higher proportion of midbrain dopaminergic neurons,which could be an ideal resource for tackling midbrain-related diseases.展开更多
Objective:Epidermal growth factor receptor(EGFR)activation was reported to upregulate programmed death-ligand 1(PD-L1)expression in lung cancer cells and subsequently contribute to immune escape,indicating its critica...Objective:Epidermal growth factor receptor(EGFR)activation was reported to upregulate programmed death-ligand 1(PD-L1)expression in lung cancer cells and subsequently contribute to immune escape,indicating its critical role in EGFR-driven lung tumors.This study characterized PD-L1 expression in patients with surgically resected EGFR-mutant non-small cell lung cancer(NSCLC).The effect of PD-L1 expression on clinical outcomes was also investigated in advanced EGFR-mutant NSCLC treated with EGFR-tyrosine kinase inhibitors(TKIs).Methods:In total,73 patients with surgically resected NSCLC and EGFR mutations were identified.PD-L1 expression and CD8+tumor-infiltrating lymphocyte(TIL)density were assessed by immunohistochemistry.A literature review of publications that assessed the predictive and prognostic value of PD-L1 expression in advanced EGFR-mutant NSCLC patients treated with EGFR-TKIs was performed.Results:Nineteen(26.0%)patients were positive for PD-L1 expression,which was significantly associated with concomitant KRAS mutation(P=0.020)and marginally associated with higher CD8+TILs density(P=0.056).Positive PD-L1 expression was associated with markedly inferior overall survival(OS)in multivariate analysis(P=0.032).The combination of PD-L1 and CD8+TILs expression could be used to stratify the population into three groups with distinct prognoses.A meta-analysis of six publications showed that positive PD-L1 expression was not associated with OS[hazard ratio(HR)=0.90;95%confidence interval(CI),0.42–1.38]or progression-free survival(HR=1.03;95 CI,0.73–1.33)in advanced EGFR-mutant NSCLC patients receiving EGFR-TKIs.Conclusions:PD-L1 expression tended to correlate with CD8+TIL expression,concomitant KRAS mutation,and poor survival in surgically resected EGFR-mutant NSCLC.PD-L1 expression was neither the predictive nor the prognostic factor in advanced EGFR-mutant NSCLC patients treated with EGFR-TKIs.展开更多
Deformation of the flexspline is the basis of analyzing tooth trajectory and designing tooth profile.Considering the tooth influence on the position of equivalent neutral layer,a piecewise method for calculating the d...Deformation of the flexspline is the basis of analyzing tooth trajectory and designing tooth profile.Considering the tooth influence on the position of equivalent neutral layer,a piecewise method for calculating the deformation of flexspline assembled with a cam wave generator is presented in this paper.Firstly,a mechanic model of a ring of uniform thickness in contact with a rigid cam is established.The displacements of the ring inside and outside an unknown wrapping angle are determined by the geometric constraints of the cam profile and the equilibrium rela-tionship,respectively.Meanwhile,the wrapping angle is solved according to the boundary conditions.The assembly forces are derived to investigate the circumferential elongation and strain.Then,considering the tooth effects on the neutral layer of flexspline,the tooth is positioned on the equivalent neutral layer,which is the non-elongation layer within one gear pitch but offset from the geometric mid-layer.The equivalent neutral layer is positioned by the empirical formula of the offset ratio,which is summarized by the orthogonal simulation on finite element models of racks.Finally,finite element models of a ring-shaped and a cup-shaped flexspline assembled with elliptical cam are established to verify the effectiveness and accuracy of the piecewise method.The results show that,compared with the geometric method,the tooth positioning deviation calculated by the piecewise method can be reduced by about 70%with a more accurate deformation description from the geometric condition and mechanic condition inside and outside the wrapping angle.展开更多
With the ever-growing application of lithium-ion batteries(LIBs), their fast-charging technology has attracted great interests of scientists. However, growth of lithium dendrites during fast charge of the bat teries w...With the ever-growing application of lithium-ion batteries(LIBs), their fast-charging technology has attracted great interests of scientists. However, growth of lithium dendrites during fast charge of the bat teries with high energy density may pose great threats to the operation and cause serious safety issues Herein, we prepared a functional separator with an ultra-thin(20 nm) layer of Au nanoparticles deposited by evaporation coating method which could regulate growth direction and morphology of the lithium dendrites, owing to nearly zero overpotential of lithium meal nucleation on lithiated Au. Once the Li den drites are about to form on the graphite anode during fast charging(or lithiation), they plate predomi nantly on the Au deposited separator rather than on the graphite. Such selective deposition does no compromise the electrochemical performance of batteries under normal cycling. More importantly, i enables the better cycling stability of batteries at fast charge condition. The Li/Graphite cells with Au nanoparticles coated separator could cycle stably with a high areal capacity retention of 90.5% over 95 cycles at the current density of 0.72 m A cm^(-2). The functional separator provides an effective strategy to adjust lithium plating position at fast charge to ensure high safety of batteries without a compromise on the energy density of LIBs.展开更多
In this paper, we present a set of best practices for workflow design and implementation for numerical weather prediction models and meteorological data service, which have been in operation in China Meteorological Ad...In this paper, we present a set of best practices for workflow design and implementation for numerical weather prediction models and meteorological data service, which have been in operation in China Meteorological Administration (CMA) for years and have been proven effective in reliably managing the complexities of large-scale meteorological related workflows. Based on the previous work on the platforms, we argue that a minimum set of guidelines including workflow scheme, module design, implementation standards and maintenance consideration during the whole establishment of the platform are highly recommended, serving to reduce the need for future maintenance and adjustment. A significant gain in performance can be achieved through the workflow-based projects. We believe that a good workflow system plays an important role in the weather forecast service, providing a useful tool for monitoring the whole process, fixing the errors, repairing a workflow, or redesigning an equivalent workflow pattern with new components.展开更多
Hepatocellular carcinoma(HCC)is the most commonly diagnosed carcinoma and one of the leading causes of cancer-related deaths worldwide.Situs inversus totalis(SIT)is a congenital condition where in the internal organs ...Hepatocellular carcinoma(HCC)is the most commonly diagnosed carcinoma and one of the leading causes of cancer-related deaths worldwide.Situs inversus totalis(SIT)is a congenital condition where in the internal organs of the abdomen and thorax lie in mirror images of their normal position.Thus far,there are very few reports on cases of SIT coexisting with HCC.Our case series is probably the largest series in world literature.The cohort of this retrospective study included a total of nine patients diagnosed with SIT-HCC and treated in our hospital between January 2013 and May 2018.Clinical characteristics,prognostic factors,and outcomes were summarized.Treatment strategies included surgery,transarterial chemoembolization,and microwave ablation.The diagnosis and treatment of patients with SIT are challenging because of organ reversion.The current treatment strategies for different stages of liver cancer are safe and feasible for patients with SIT-HCC.展开更多
Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance(SPR)sensors.In this paper,we report a method circulating the dopamine aqueous s...Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance(SPR)sensors.In this paper,we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip.The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis.These opera-tions are all performed under flow condition in the fuidic system,and have the advantages of easy implementation,less time consuming,and low cost,because the reagents and devices used in the operations are routinely applied in most laboratories.In this study,the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.展开更多
Objective: The aim of our study was to compare the efficacy and toxicities of vinorelbine plus cisplatin(NP) regimen with that of vinorelbine plus capecitabine(NX) regimen in the treatment of anthracycline- and taxane...Objective: The aim of our study was to compare the efficacy and toxicities of vinorelbine plus cisplatin(NP) regimen with that of vinorelbine plus capecitabine(NX) regimen in the treatment of anthracycline- and taxane-refractory advanced breast cancer. Methods: Forty-six patients with anthracycline- and taxane-refractory advanced breast cancer were equally randomized into a NP group(n = 23) and a NX group(n = 23). Response rates and toxicities were evaluated after 2 cycles of chemotherapy. Results: The overall response rate were 48.0% in both groups. There were no significant differences in disease control rates(78.0% vs. 83%) or 1-year survival rates(54.6% vs. 55.9%). The main adverse events were bone marrow depression and gastrointestinal reaction, and no significant difference was found in toxicities between the groups. Conclusion: For anthracycline- and taxane-refractory advanced breast cancer, NP and NX regimens exerted similar curative effects with acceptable toxicity.展开更多
The relationships between vegetation and environmental factors have always been a core concern of ecologists.The dynamic characteristics of plant communities during the growing season can directly reflect these relati...The relationships between vegetation and environmental factors have always been a core concern of ecologists.The dynamic characteristics of plant communities during the growing season can directly reflect these relation-ships,so we examined this issue for three typical ecosystems on the Tibetan Plateau.During the growing season,the dominant species remained stable while non-dominant species changed significantly in the alpine meadow and alpine steppe and a mono-dominant community was found in the temperate desert shrub.Due to the seasonal variations of temperature and soil water content,plant species diversity varied significantly during the growing season.Patrick richness,Pielou evenness and Simpson diversity indices differed significantly in the alpine meadow and alpine steppe.The total biomass of these three ecosystems was the largest during the middle growing season.Biomass was greater in the alpine meadow than the alpine steeps or temperature desert.The root-to-shoot ratio was the lowest during the middle growing season for the alpine meadow and alpine steppe and largest during the early growing season for temperate desert shrub.RDA showed the belowground and total biomass were greatly affected by soil physicochemical factors.Multiple linear stepwise regression showed the above ground biomass was greatly affected by relative atmospheric humidity and belowground and total biomass were greatly affected by soil organic carbon,total nitrogen at 0-20 cm soil depth and pH at 10-20 cm soil depth.These findings pro-vide insights into understanding the relationships between vegetation and environmental factors and promote the sustainable utilization of local grasslands on the Tibetan Plateau.展开更多
China Meteorological Administration (CMA) has a long history of using High Performance Computing System (HPCS) for over three decades. CMA HPCS investment provides reliable HPC capabilities essential to run Numerical ...China Meteorological Administration (CMA) has a long history of using High Performance Computing System (HPCS) for over three decades. CMA HPCS investment provides reliable HPC capabilities essential to run Numerical Weather Prediction (NWP) models and climate models, generating millions of weather guidance products daily and providing support for Coupled Model Inter-comparison Project Phase 5 (CMIP5). Monitoring the HPCS and analyzing the resource usage can improve the performance and reliability for our users, which require a good understanding of failure characteristics. Large-scale studies of failures in real production systems are scarce. This paper collects, analyzes and studies all the failures occurring during the HPC operation period, especially focusing on studying the relationship between HPCS and NWP applications. Also, we present the challenges for a more effective monitoring system development and summarize the useful maintenance strategies. This step may have considerable effects on the performance of online failure prediction of HPC and better performance in future.展开更多
Background:The role of autophagy in the formation of hypertrophic scars(HS)remains unclear.This study aimed to explore the role and potential mechanism of autophagy during the development of HS.Methods:RNA and protein...Background:The role of autophagy in the formation of hypertrophic scars(HS)remains unclear.This study aimed to explore the role and potential mechanism of autophagy during the development of HS.Methods:RNA and protein expression levels of Beclin-1,p62,and LC3II in normal skin tissues and HS specimens from different patients were examined.Autophagy inducers and inhibitors were used to cure established HS in rabbit ears,and the expression of Beclin-1,p62,and LC3II at the RNA and protein level was determined.Lastly,the effects of autophagy inducers and inhibitors on HS development were analyzed.Results:Compared to normal skin tissues,the expression of LC3II and Beclin-1 was higher(P<0.05),while that of p62 was lower(P<0.05)in HS tissues.In addition,the LC3II/LC3I ratio was increased during HS formation,and the altered expression of the three proteins stabilized after one year.Administration of autophagy inducers enhanced the formation of HS as well as the expression levels of LC3II and Beclin-1 but decreased p62 expression.Meanwhile,administration of autophagy inhibitors increased the expression of LC3II,Beclin-1,and p62,along with reduced HS formation.Conclusion:Autophagic activity increased during HS initiation and subsequent stabilization.In addition,autophagy inhibitors were able to inhibit HS formation by suppressing autophagy,whereas autophagy inducers promoted scar hyperplasia by enhancing autophagy。展开更多
Electrosynthesis of hydrogen peroxide(H2O2)is an on-site method that enables independent distribution applications in many fields due to its small-scale and sustainable features.The crucial point remains developing hi...Electrosynthesis of hydrogen peroxide(H2O2)is an on-site method that enables independent distribution applications in many fields due to its small-scale and sustainable features.The crucial point remains developing highly active,selective and cost-effective electrocatalysts.The electrosynthesis of H2O2 in acidic media is more practical owing to its stability and no need for further purification.We herein report a phosphorus and selenium tuning Co-based non-precious catalyst(CoPSe)toward two-electron oxygen reduction reaction(2e–ORR)to produce H2O2 in acidic media.The starting point of using both P and Se is finding a balance between strong ORR activity of CoSe and weak activity of CoP.The results demonstrated that the CoPSe catalyst exhibited the optimized 2e–ORR activity compared with CoP and CoSe.It disclosed an onset potential of 0.68 V and the H2O2 selectivity 76%-85%in a wide potential range(0–0.5 V).Notably,the CoPSe catalyst overcomes a significant challenge of a narrow-range selectivity for transitionmetal based 2e–ORR catalysts.Finally,combining with electro-Fenton reaction,an on-site system was constructed for efficient degradation of organic pollutants.This work provides a promising non-precious Co-based electrocatalyst for the electrosynthesis of H2O2 in acidic media.展开更多
Background:Programmed death 1(PD-1)blockade plus chemotherapy has become the new first-line standard of care for patients with advanced non-small-cell lung cancer(NSCLC).Yet not all NSCLC patients benefit from this re...Background:Programmed death 1(PD-1)blockade plus chemotherapy has become the new first-line standard of care for patients with advanced non-small-cell lung cancer(NSCLC).Yet not all NSCLC patients benefit from this regimen.This study aimed to investigate the predictors of PD-1 blockade plus chemotherapy in untreated advanced NSCLC.Methods:We integrated clinical,genomic,and survival data from 287 patients with untreated advanced NSCLC who were enrolled in one of five registered phase 3 trials and received PD-1 blockade plus chemotherapy or chemotherapy alone.We randomly assigned these patients into a discovery cohort(n=125),a validation cohort(n=82),and a control cohort(n=80).The candidate genes that could predict the response to PD-1 blockade plus chemotherapy were identified using data from the discovery cohort and their predictive values were then evaluated in the three cohorts.Immune deconvolution was conducted using transcriptome data of 1014 NSCLC patients from The Cancer Genome Atlas dataset.Results:A genomic variation signature,in which one or more of the 15 candidate genes were altered,was correlated with significantly inferior response rates and survival outcomes in patients treated with first-line PD-1 blockade plus chemotherapy in both discovery and validation cohorts.Its predictive value held in multivariate analyses when adjusted for baseline parameters,programmed cell death ligand 1(PD-L1)expression level,and tumor mutation burden.Moreover,applying both the 15-gene panel and PD-L1 expression level produced better performance than either alone in predicting benefit from this treatment combination.Immune landscape analyses revealed that tumors with one or more variation in the 15-gene panel were associated with few immune infiltrates,indicating an immune-desert tumor microenvironment.Conclusion:These findings indicate that a 15-gene panel can serve as a negative prediction biomarker for first-line PD-1 blockade plus chemotherapy in patients with advanced NSCLC.展开更多
To the Editor:In recent years,impressive outcomes have been achieved in patients harboring human epidermal growth factor receptor 2(HER2)mutations,which accounts for 1%to 4%of cases in non-small cell lung cancer(NSCLC).
Mesoporous Mn-Sn bimetallic oxide (BO) nanocubes with sizes of 15-30 run show outstanding stable and reversible capacities in lithium ion batteries CLIBs), reaching 856.8 mAh.g-1 after 400 cycles at 500 mA·g^-...Mesoporous Mn-Sn bimetallic oxide (BO) nanocubes with sizes of 15-30 run show outstanding stable and reversible capacities in lithium ion batteries CLIBs), reaching 856.8 mAh.g-1 after 400 cycles at 500 mA·g^-1 and 506 mAh·g^-1 after 850 cycles at 1,000 mA·g^-1. The prelimLnary investigation of the reaction mechanism, based on X-ray diffraction measurements, indicates the occurrence of both conversion and alloying-dealloying reactions in the Mn-Sn bimetallic oxide electrode. Moreover, Mn-Sn BO//LiCoO2 Li-ion full cells were successfully assembled for the first time, and found to deliver a relatively high energy density of 176.25 Wh·kg^-1 at 16.35 W·kg^-1 (based on the total weight of anode and cathode materials). The superior long-term stability of these materials might be attributed to their nanoscale size and unique mesoporous nanocubic structure, which provide short Li^+ diffusion pathways and a high contact area between electrolyte and active material. In addition, the Mn-Sn BOs could be used as advanced sulfur hosts for lithium-sulfur batteries, owing to their adequate mesoporous structure and relatively strong chemisorption of lithium polysulfide. The present results thus highlight the promising potential of mesoporous Mn-Sn bimetallic oxides for application in Li-ion and Li-S batteries.展开更多
Broadband near-infrared phosphors are highly desirable for food testing.Targeted Ca_(2)LuHf_(2)Al_(3)O_(12):Cr^(3+)(CLHA:Cr^(3+)) and Ca_(2)LuHf_(2)Al_(3)O_(12):Ce^(3+),Cr^(3+)(CLHA:Ce^(3+),Cr^(^(3+))) phosphors were ...Broadband near-infrared phosphors are highly desirable for food testing.Targeted Ca_(2)LuHf_(2)Al_(3)O_(12):Cr^(3+)(CLHA:Cr^(3+)) and Ca_(2)LuHf_(2)Al_(3)O_(12):Ce^(3+),Cr^(3+)(CLHA:Ce^(3+),Cr^(^(3+))) phosphors were synthesized by the conventional high-temperature solid state reaction.The CLHA:Cr^(3+)phosphor,with a good thermal stability,shows a red shift owing to radiation reabsorption and non-radiative transition with increasing Cr^(3+)content.For co-doped sample,the emission intensity of Cr^(3+)can be enhanced by three times due to the energy transfer from Ce^(3+)to Cr^(3+),which can be evidenced by a significant overlap between the PLE of Cr^(3+)single-doped phosphor and the PL of Ce^(3+)single-doped phosphor.In addition,the mechanism of energy transfer is identified as a quadrupole-quadrupole interaction according to decay Lifetime and Dexter’s energy transfer formula.The broadband NIR emission peaked at 775 nm of CLHA:Cr^(3+),Ce^(3+)phosphor shows a bright prospect in nondestructive quality-control analysis systems for food.展开更多
Cobalt and nitrogen codoped carbon materials(Co-N-C) were fabricated by pyrolysis of the mixture of poly(4-vinylpyridine) and cobalt chloride using SiO_2 nanoparticles as hard template, which were the first transition...Cobalt and nitrogen codoped carbon materials(Co-N-C) were fabricated by pyrolysis of the mixture of poly(4-vinylpyridine) and cobalt chloride using SiO_2 nanoparticles as hard template, which were the first transition metal/nitrogen-codoped carbon bifunctional electrocatalyst derived from noncarbonizable polymer for ORR and HER. The as-made Co-N-C possessed hierarchical pore structure and high specific surface area, achieving excellent electrocatalytic performances for ORR and HER. Its ORR catalytic performances were comparable to those of Pt/C catalyst and its HER catalytic performances were superior to those of most doped carbon catalysts in KOH electrolyte. Moreover, its bifunctional electrocatalytic performances for ORR and HER were better than those of most bifunctional doped carbon catalysts in alkaline electrolyte.展开更多
BCL-2 gene as well as its products is recognized as a promising target for the molecular targeted therapy of tumors.However,due to certain defense measures of tumor cells,the therapeutic effect based on the gene silen...BCL-2 gene as well as its products is recognized as a promising target for the molecular targeted therapy of tumors.However,due to certain defense measures of tumor cells,the therapeutic effect based on the gene silencing of BCL-2 is greatly reduced.Here we fabricate a smart response nucleic acid therapeutic that could silence the gene effectively through a dual-targeted and cascade-enhanced strategy.In brief,nano-graphene oxide(GO),working as a nano-carrier,is loaded with a well-designed DNAzyme,which can target and silence the BCL-2 mRNA.Furthermore,upon binding with the BCL-2 mRNA,the enzymatic activity of the DNAzyme can be initiated,cutting a substrate oligonucleotide to produce an anti-nucleolin aptamer AS1411.Nucleolin,a nucleolar phosphoprotein,is known as a stabilizer of BCL-2 mRNA.Via binding and inactivating the nucleolin,AS1411 can destabilize BCL-2 mRNA.By this means of simultaneously targeting mRNA and its stabilizer in an integrated system,effective silencing of the BCL-2 gene of tumor cells is achieved at both the cellular and in vivo levels.After being dosed with this nucleic acid therapeutic and without any chemotherapeutics,apoptosis of tumor cells at the cellular level and apparent shrinkage of tumors in vivo are observed.By labeling a molecular beacon on the substrate of DNAzyme,visualization of the enzymatic activity as well as the tumor in vivo can be also achieved.Our work presents a pure bio-therapeutic strategy that has positive implications for enhancing tumor treatment and avoiding side effects of chemotherapeutics.展开更多
Lithium-sulfur (Li-S) battery as one of the most attractive candidates for energy storage systems has attracted extensive interests.Herein,for the first time,hierarchical flower-like cobalt phosphosulfide architecture...Lithium-sulfur (Li-S) battery as one of the most attractive candidates for energy storage systems has attracted extensive interests.Herein,for the first time,hierarchical flower-like cobalt phosphosulfide architectures (defined as "CoSP") derived from Prussian blue analogue (PBA) was fabricated through the conversion of Co-based PBA in PxSy atmosphere.The as-prepared polar CoSP could effectively trap polysulfides through the formation of strong chemical bonds.In addition,after the combination of CoSP with high conductive rGO,the obtained CoSP/rGO as sulfur host material exhibits ultralow capacity decay rate of 0.046% per cycle over 900 cycles at a current density of 1 C.The excellent performance could be attributed to the shortened lithium diffusion pathways,fastened electron transport ability during polysulfide conversion,and increased much more anchor active sites to polysulfides,which is expected to be a promising material for Li-S batteries.It is believed that the as-prepared CoSP/rGO architectures will shed light on the development of novel promising materials for Li-S batteries with high cycle stability.展开更多
基金supported by the National Natural Science Foundation of China,No.81771222(to LS)Guangzhou Key Research Program on Brain Science,Nos.202007030011,202206060001(to LS)the Program of Introducing Talents of Discipline to Universities of China,No.B14036(to KFS)。
文摘Midbrain dopaminergic neurons play an important role in the etiology of neurodevelopmental and neurodegenerative diseases.They also represent a potential source of transplanted cells for therapeutic applications.In vitro differentiation of functional midbrain dopaminergic neurons provides an accessible platform to study midbrain neuronal dysfunction and can be used to examine obstacles to dopaminergic neuronal development.Emerging evidence and impressive advances in human induced pluripotent stem cells,with tuned neural induction and differentiation protocols,makes the production of induced pluripotent stem cell-derived dopaminergic neurons feasible.Using SB431542 and dorsomorphin dual inhibitor in an induced pluripotent stem cell-derived neural induction protocol,we obtained multiple subtypes of neurons,including 20%tyrosine hydroxylase-positive dopaminergic neurons.To obtain more dopaminergic neurons,we next added sonic hedgehog(SHH)and fibroblast growth factor 8(FGF8)on day 8 of induction.This increased the proportion of dopaminergic neurons,up to 75%tyrosine hydroxylase-positive neurons,with 15%tyrosine hydroxylase and forkhead box protein A2(FOXA2)co-expressing neurons.We further optimized the induction protocol by applying the small molecule inhibitor,CHIR99021(CHIR).This helped facilitate the generation of midbrain dopaminergic neurons,and we obtained 31-74%midbrain dopaminergic neurons based on tyrosine hydroxylase and FOXA2 staining.Thus,we have established three induction protocols for dopaminergic neurons.Based on tyrosine hydroxylase and FOXA2 immunostaining analysis,the CHIR,SHH,and FGF8 combined protocol produces a much higher proportion of midbrain dopaminergic neurons,which could be an ideal resource for tackling midbrain-related diseases.
基金supported in part by grants from the National Natural Science Foundation of China(Grant No.81672286,81772467 and 81874036)“Shuguang Program”supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission(Grant No.16SG18)the Chronic Diseases Program of Shanghai Shen Kang Pharmaceutical Development Co.Ltd(Grant No.SHDC 12015314)
文摘Objective:Epidermal growth factor receptor(EGFR)activation was reported to upregulate programmed death-ligand 1(PD-L1)expression in lung cancer cells and subsequently contribute to immune escape,indicating its critical role in EGFR-driven lung tumors.This study characterized PD-L1 expression in patients with surgically resected EGFR-mutant non-small cell lung cancer(NSCLC).The effect of PD-L1 expression on clinical outcomes was also investigated in advanced EGFR-mutant NSCLC treated with EGFR-tyrosine kinase inhibitors(TKIs).Methods:In total,73 patients with surgically resected NSCLC and EGFR mutations were identified.PD-L1 expression and CD8+tumor-infiltrating lymphocyte(TIL)density were assessed by immunohistochemistry.A literature review of publications that assessed the predictive and prognostic value of PD-L1 expression in advanced EGFR-mutant NSCLC patients treated with EGFR-TKIs was performed.Results:Nineteen(26.0%)patients were positive for PD-L1 expression,which was significantly associated with concomitant KRAS mutation(P=0.020)and marginally associated with higher CD8+TILs density(P=0.056).Positive PD-L1 expression was associated with markedly inferior overall survival(OS)in multivariate analysis(P=0.032).The combination of PD-L1 and CD8+TILs expression could be used to stratify the population into three groups with distinct prognoses.A meta-analysis of six publications showed that positive PD-L1 expression was not associated with OS[hazard ratio(HR)=0.90;95%confidence interval(CI),0.42–1.38]or progression-free survival(HR=1.03;95 CI,0.73–1.33)in advanced EGFR-mutant NSCLC patients receiving EGFR-TKIs.Conclusions:PD-L1 expression tended to correlate with CD8+TIL expression,concomitant KRAS mutation,and poor survival in surgically resected EGFR-mutant NSCLC.PD-L1 expression was neither the predictive nor the prognostic factor in advanced EGFR-mutant NSCLC patients treated with EGFR-TKIs.
基金Supported by National Natural Science Foundation of China(Grant No.51575390)Tianjin Municipal Natural Science Foundation of China(Grant Nos.19JCZDJC38700,18JCZDJC39000).
文摘Deformation of the flexspline is the basis of analyzing tooth trajectory and designing tooth profile.Considering the tooth influence on the position of equivalent neutral layer,a piecewise method for calculating the deformation of flexspline assembled with a cam wave generator is presented in this paper.Firstly,a mechanic model of a ring of uniform thickness in contact with a rigid cam is established.The displacements of the ring inside and outside an unknown wrapping angle are determined by the geometric constraints of the cam profile and the equilibrium rela-tionship,respectively.Meanwhile,the wrapping angle is solved according to the boundary conditions.The assembly forces are derived to investigate the circumferential elongation and strain.Then,considering the tooth effects on the neutral layer of flexspline,the tooth is positioned on the equivalent neutral layer,which is the non-elongation layer within one gear pitch but offset from the geometric mid-layer.The equivalent neutral layer is positioned by the empirical formula of the offset ratio,which is summarized by the orthogonal simulation on finite element models of racks.Finally,finite element models of a ring-shaped and a cup-shaped flexspline assembled with elliptical cam are established to verify the effectiveness and accuracy of the piecewise method.The results show that,compared with the geometric method,the tooth positioning deviation calculated by the piecewise method can be reduced by about 70%with a more accurate deformation description from the geometric condition and mechanic condition inside and outside the wrapping angle.
基金supported by the National Key Research and Development Program(2019YFC0810703)the National Natural Science Foundation of China(22071133)the China Postdoctoral Science Foundation(2020M680581)。
文摘With the ever-growing application of lithium-ion batteries(LIBs), their fast-charging technology has attracted great interests of scientists. However, growth of lithium dendrites during fast charge of the bat teries with high energy density may pose great threats to the operation and cause serious safety issues Herein, we prepared a functional separator with an ultra-thin(20 nm) layer of Au nanoparticles deposited by evaporation coating method which could regulate growth direction and morphology of the lithium dendrites, owing to nearly zero overpotential of lithium meal nucleation on lithiated Au. Once the Li den drites are about to form on the graphite anode during fast charging(or lithiation), they plate predomi nantly on the Au deposited separator rather than on the graphite. Such selective deposition does no compromise the electrochemical performance of batteries under normal cycling. More importantly, i enables the better cycling stability of batteries at fast charge condition. The Li/Graphite cells with Au nanoparticles coated separator could cycle stably with a high areal capacity retention of 90.5% over 95 cycles at the current density of 0.72 m A cm^(-2). The functional separator provides an effective strategy to adjust lithium plating position at fast charge to ensure high safety of batteries without a compromise on the energy density of LIBs.
文摘In this paper, we present a set of best practices for workflow design and implementation for numerical weather prediction models and meteorological data service, which have been in operation in China Meteorological Administration (CMA) for years and have been proven effective in reliably managing the complexities of large-scale meteorological related workflows. Based on the previous work on the platforms, we argue that a minimum set of guidelines including workflow scheme, module design, implementation standards and maintenance consideration during the whole establishment of the platform are highly recommended, serving to reduce the need for future maintenance and adjustment. A significant gain in performance can be achieved through the workflow-based projects. We believe that a good workflow system plays an important role in the weather forecast service, providing a useful tool for monitoring the whole process, fixing the errors, repairing a workflow, or redesigning an equivalent workflow pattern with new components.
文摘Hepatocellular carcinoma(HCC)is the most commonly diagnosed carcinoma and one of the leading causes of cancer-related deaths worldwide.Situs inversus totalis(SIT)is a congenital condition where in the internal organs of the abdomen and thorax lie in mirror images of their normal position.Thus far,there are very few reports on cases of SIT coexisting with HCC.Our case series is probably the largest series in world literature.The cohort of this retrospective study included a total of nine patients diagnosed with SIT-HCC and treated in our hospital between January 2013 and May 2018.Clinical characteristics,prognostic factors,and outcomes were summarized.Treatment strategies included surgery,transarterial chemoembolization,and microwave ablation.The diagnosis and treatment of patients with SIT are challenging because of organ reversion.The current treatment strategies for different stages of liver cancer are safe and feasible for patients with SIT-HCC.
基金This research was made possible with the financial support from NSFC China(61275188,61378089,81470029,61361160416)the 863 project,China,the Technology Development Program of Shenzhen City,the Committee of Science and Technology Innovation of Shenzhen(JCYJ20140902110354241)Science and Technology Project of Guangdong Province(2015A010106002).
文摘Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance(SPR)sensors.In this paper,we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip.The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis.These opera-tions are all performed under flow condition in the fuidic system,and have the advantages of easy implementation,less time consuming,and low cost,because the reagents and devices used in the operations are routinely applied in most laboratories.In this study,the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.
基金Supported by a grant from the Key Project of National 12th Five-year Research Program of China(No.2012ZX0903016-002)
文摘Objective: The aim of our study was to compare the efficacy and toxicities of vinorelbine plus cisplatin(NP) regimen with that of vinorelbine plus capecitabine(NX) regimen in the treatment of anthracycline- and taxane-refractory advanced breast cancer. Methods: Forty-six patients with anthracycline- and taxane-refractory advanced breast cancer were equally randomized into a NP group(n = 23) and a NX group(n = 23). Response rates and toxicities were evaluated after 2 cycles of chemotherapy. Results: The overall response rate were 48.0% in both groups. There were no significant differences in disease control rates(78.0% vs. 83%) or 1-year survival rates(54.6% vs. 55.9%). The main adverse events were bone marrow depression and gastrointestinal reaction, and no significant difference was found in toxicities between the groups. Conclusion: For anthracycline- and taxane-refractory advanced breast cancer, NP and NX regimens exerted similar curative effects with acceptable toxicity.
基金This research was funded by the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0405)National Natural Science Foundation of China(Grant No.41771325,41171445).
文摘The relationships between vegetation and environmental factors have always been a core concern of ecologists.The dynamic characteristics of plant communities during the growing season can directly reflect these relation-ships,so we examined this issue for three typical ecosystems on the Tibetan Plateau.During the growing season,the dominant species remained stable while non-dominant species changed significantly in the alpine meadow and alpine steppe and a mono-dominant community was found in the temperate desert shrub.Due to the seasonal variations of temperature and soil water content,plant species diversity varied significantly during the growing season.Patrick richness,Pielou evenness and Simpson diversity indices differed significantly in the alpine meadow and alpine steppe.The total biomass of these three ecosystems was the largest during the middle growing season.Biomass was greater in the alpine meadow than the alpine steeps or temperature desert.The root-to-shoot ratio was the lowest during the middle growing season for the alpine meadow and alpine steppe and largest during the early growing season for temperate desert shrub.RDA showed the belowground and total biomass were greatly affected by soil physicochemical factors.Multiple linear stepwise regression showed the above ground biomass was greatly affected by relative atmospheric humidity and belowground and total biomass were greatly affected by soil organic carbon,total nitrogen at 0-20 cm soil depth and pH at 10-20 cm soil depth.These findings pro-vide insights into understanding the relationships between vegetation and environmental factors and promote the sustainable utilization of local grasslands on the Tibetan Plateau.
文摘China Meteorological Administration (CMA) has a long history of using High Performance Computing System (HPCS) for over three decades. CMA HPCS investment provides reliable HPC capabilities essential to run Numerical Weather Prediction (NWP) models and climate models, generating millions of weather guidance products daily and providing support for Coupled Model Inter-comparison Project Phase 5 (CMIP5). Monitoring the HPCS and analyzing the resource usage can improve the performance and reliability for our users, which require a good understanding of failure characteristics. Large-scale studies of failures in real production systems are scarce. This paper collects, analyzes and studies all the failures occurring during the HPC operation period, especially focusing on studying the relationship between HPCS and NWP applications. Also, we present the challenges for a more effective monitoring system development and summarize the useful maintenance strategies. This step may have considerable effects on the performance of online failure prediction of HPC and better performance in future.
基金the National Natural Science Foundation of China(grant no.81872219)Science and Technology Project of Hunan Provincial Health Commission(grant no.B2015-040)+2 种基金Major Scientific and Technological Projects in Hunan Province(grant no.2019SK1010)2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant(grant nos.2020LKSFG18B,2020LKSFG02E)Guangdong University Innovation Team Project(grant no.2021KCXTD047).
文摘Background:The role of autophagy in the formation of hypertrophic scars(HS)remains unclear.This study aimed to explore the role and potential mechanism of autophagy during the development of HS.Methods:RNA and protein expression levels of Beclin-1,p62,and LC3II in normal skin tissues and HS specimens from different patients were examined.Autophagy inducers and inhibitors were used to cure established HS in rabbit ears,and the expression of Beclin-1,p62,and LC3II at the RNA and protein level was determined.Lastly,the effects of autophagy inducers and inhibitors on HS development were analyzed.Results:Compared to normal skin tissues,the expression of LC3II and Beclin-1 was higher(P<0.05),while that of p62 was lower(P<0.05)in HS tissues.In addition,the LC3II/LC3I ratio was increased during HS formation,and the altered expression of the three proteins stabilized after one year.Administration of autophagy inducers enhanced the formation of HS as well as the expression levels of LC3II and Beclin-1 but decreased p62 expression.Meanwhile,administration of autophagy inhibitors increased the expression of LC3II,Beclin-1,and p62,along with reduced HS formation.Conclusion:Autophagic activity increased during HS initiation and subsequent stabilization.In addition,autophagy inhibitors were able to inhibit HS formation by suppressing autophagy,whereas autophagy inducers promoted scar hyperplasia by enhancing autophagy。
基金the National Natural Science Foundation of China(Nos.21805052,21974031,2278092)Science and Technology Research Project of Guangzhou(Nos.202102020787 and 202201000002)+2 种基金Department of Science&Technology of Guangdong Province(No.2022A156)Key Discipline of Materials Science and Engineering,Bureau of Education of Guangzhou(No.20225546)the Innovation&Entrepreneurship for the College Students of Guangzhou University(No.XJ202111078175).
文摘Electrosynthesis of hydrogen peroxide(H2O2)is an on-site method that enables independent distribution applications in many fields due to its small-scale and sustainable features.The crucial point remains developing highly active,selective and cost-effective electrocatalysts.The electrosynthesis of H2O2 in acidic media is more practical owing to its stability and no need for further purification.We herein report a phosphorus and selenium tuning Co-based non-precious catalyst(CoPSe)toward two-electron oxygen reduction reaction(2e–ORR)to produce H2O2 in acidic media.The starting point of using both P and Se is finding a balance between strong ORR activity of CoSe and weak activity of CoP.The results demonstrated that the CoPSe catalyst exhibited the optimized 2e–ORR activity compared with CoP and CoSe.It disclosed an onset potential of 0.68 V and the H2O2 selectivity 76%-85%in a wide potential range(0–0.5 V).Notably,the CoPSe catalyst overcomes a significant challenge of a narrow-range selectivity for transitionmetal based 2e–ORR catalysts.Finally,combining with electro-Fenton reaction,an on-site system was constructed for efficient degradation of organic pollutants.This work provides a promising non-precious Co-based electrocatalyst for the electrosynthesis of H2O2 in acidic media.
基金supported by grants from the National Natural Science Foundation of China(Nos.82102859,82172869,82141101,82272875,and 12126605)the Shanghai Rising-Star Program(Nos.23QA1408000 and 22QA1407800)+1 种基金the Shanghai"Rising Stars of Medical Talent"Youth Development Program Youth Medical Talents—Specialist Program,the Original Exploration Project of Shanghai Natural Science Foundation(No.23ZR1480600)the Health and Family Planning Commission of Shanghai Municipality(No.20224Y0067)
文摘Background:Programmed death 1(PD-1)blockade plus chemotherapy has become the new first-line standard of care for patients with advanced non-small-cell lung cancer(NSCLC).Yet not all NSCLC patients benefit from this regimen.This study aimed to investigate the predictors of PD-1 blockade plus chemotherapy in untreated advanced NSCLC.Methods:We integrated clinical,genomic,and survival data from 287 patients with untreated advanced NSCLC who were enrolled in one of five registered phase 3 trials and received PD-1 blockade plus chemotherapy or chemotherapy alone.We randomly assigned these patients into a discovery cohort(n=125),a validation cohort(n=82),and a control cohort(n=80).The candidate genes that could predict the response to PD-1 blockade plus chemotherapy were identified using data from the discovery cohort and their predictive values were then evaluated in the three cohorts.Immune deconvolution was conducted using transcriptome data of 1014 NSCLC patients from The Cancer Genome Atlas dataset.Results:A genomic variation signature,in which one or more of the 15 candidate genes were altered,was correlated with significantly inferior response rates and survival outcomes in patients treated with first-line PD-1 blockade plus chemotherapy in both discovery and validation cohorts.Its predictive value held in multivariate analyses when adjusted for baseline parameters,programmed cell death ligand 1(PD-L1)expression level,and tumor mutation burden.Moreover,applying both the 15-gene panel and PD-L1 expression level produced better performance than either alone in predicting benefit from this treatment combination.Immune landscape analyses revealed that tumors with one or more variation in the 15-gene panel were associated with few immune infiltrates,indicating an immune-desert tumor microenvironment.Conclusion:These findings indicate that a 15-gene panel can serve as a negative prediction biomarker for first-line PD-1 blockade plus chemotherapy in patients with advanced NSCLC.
基金National Natural Science Foundation of China(Nos.81972167,82172869 and 82002419)Shanghai Shenkang Hospital Development Center(No.SHDC12019133)+1 种基金Clinical Research Foundation of Shanghai Pulmonary Hospital(No.FKLY20008)Shanghai Innovative Collaboration Project(No.2020CXJQ02)
文摘To the Editor:In recent years,impressive outcomes have been achieved in patients harboring human epidermal growth factor receptor 2(HER2)mutations,which accounts for 1%to 4%of cases in non-small cell lung cancer(NSCLC).
基金Thanks for the financial support from the National Nature Science Foundation of China (No. 21471091), Academy of Sciences large apparatus United Fund (No. 11179043), the Fundamental Research Funds of Shandong University (No. 2015JC007), and the Taishan Scholar Project of Shandong Province (No. ts201511004).
文摘Mesoporous Mn-Sn bimetallic oxide (BO) nanocubes with sizes of 15-30 run show outstanding stable and reversible capacities in lithium ion batteries CLIBs), reaching 856.8 mAh.g-1 after 400 cycles at 500 mA·g^-1 and 506 mAh·g^-1 after 850 cycles at 1,000 mA·g^-1. The prelimLnary investigation of the reaction mechanism, based on X-ray diffraction measurements, indicates the occurrence of both conversion and alloying-dealloying reactions in the Mn-Sn bimetallic oxide electrode. Moreover, Mn-Sn BO//LiCoO2 Li-ion full cells were successfully assembled for the first time, and found to deliver a relatively high energy density of 176.25 Wh·kg^-1 at 16.35 W·kg^-1 (based on the total weight of anode and cathode materials). The superior long-term stability of these materials might be attributed to their nanoscale size and unique mesoporous nanocubic structure, which provide short Li^+ diffusion pathways and a high contact area between electrolyte and active material. In addition, the Mn-Sn BOs could be used as advanced sulfur hosts for lithium-sulfur batteries, owing to their adequate mesoporous structure and relatively strong chemisorption of lithium polysulfide. The present results thus highlight the promising potential of mesoporous Mn-Sn bimetallic oxides for application in Li-ion and Li-S batteries.
基金Project supported by the National Key Research and Development Program of China(2016YFB400605)。
文摘Broadband near-infrared phosphors are highly desirable for food testing.Targeted Ca_(2)LuHf_(2)Al_(3)O_(12):Cr^(3+)(CLHA:Cr^(3+)) and Ca_(2)LuHf_(2)Al_(3)O_(12):Ce^(3+),Cr^(3+)(CLHA:Ce^(3+),Cr^(^(3+))) phosphors were synthesized by the conventional high-temperature solid state reaction.The CLHA:Cr^(3+)phosphor,with a good thermal stability,shows a red shift owing to radiation reabsorption and non-radiative transition with increasing Cr^(3+)content.For co-doped sample,the emission intensity of Cr^(3+)can be enhanced by three times due to the energy transfer from Ce^(3+)to Cr^(3+),which can be evidenced by a significant overlap between the PLE of Cr^(3+)single-doped phosphor and the PL of Ce^(3+)single-doped phosphor.In addition,the mechanism of energy transfer is identified as a quadrupole-quadrupole interaction according to decay Lifetime and Dexter’s energy transfer formula.The broadband NIR emission peaked at 775 nm of CLHA:Cr^(3+),Ce^(3+)phosphor shows a bright prospect in nondestructive quality-control analysis systems for food.
基金supported by the National Natural Science Foundation of China(Nos.21774073,51690151,21404071,and 21320102006)the National Basic Research Program (No. 2016YFA0201500)
文摘Cobalt and nitrogen codoped carbon materials(Co-N-C) were fabricated by pyrolysis of the mixture of poly(4-vinylpyridine) and cobalt chloride using SiO_2 nanoparticles as hard template, which were the first transition metal/nitrogen-codoped carbon bifunctional electrocatalyst derived from noncarbonizable polymer for ORR and HER. The as-made Co-N-C possessed hierarchical pore structure and high specific surface area, achieving excellent electrocatalytic performances for ORR and HER. Its ORR catalytic performances were comparable to those of Pt/C catalyst and its HER catalytic performances were superior to those of most doped carbon catalysts in KOH electrolyte. Moreover, its bifunctional electrocatalytic performances for ORR and HER were better than those of most bifunctional doped carbon catalysts in alkaline electrolyte.
基金This work was supported by the National Natural Science Foundation of China(No.21575088)the Natural Science Foundation of Shanghai(No.19ZR1474200).
文摘BCL-2 gene as well as its products is recognized as a promising target for the molecular targeted therapy of tumors.However,due to certain defense measures of tumor cells,the therapeutic effect based on the gene silencing of BCL-2 is greatly reduced.Here we fabricate a smart response nucleic acid therapeutic that could silence the gene effectively through a dual-targeted and cascade-enhanced strategy.In brief,nano-graphene oxide(GO),working as a nano-carrier,is loaded with a well-designed DNAzyme,which can target and silence the BCL-2 mRNA.Furthermore,upon binding with the BCL-2 mRNA,the enzymatic activity of the DNAzyme can be initiated,cutting a substrate oligonucleotide to produce an anti-nucleolin aptamer AS1411.Nucleolin,a nucleolar phosphoprotein,is known as a stabilizer of BCL-2 mRNA.Via binding and inactivating the nucleolin,AS1411 can destabilize BCL-2 mRNA.By this means of simultaneously targeting mRNA and its stabilizer in an integrated system,effective silencing of the BCL-2 gene of tumor cells is achieved at both the cellular and in vivo levels.After being dosed with this nucleic acid therapeutic and without any chemotherapeutics,apoptosis of tumor cells at the cellular level and apparent shrinkage of tumors in vivo are observed.By labeling a molecular beacon on the substrate of DNAzyme,visualization of the enzymatic activity as well as the tumor in vivo can be also achieved.Our work presents a pure bio-therapeutic strategy that has positive implications for enhancing tumor treatment and avoiding side effects of chemotherapeutics.
基金the Academy of Sciences large apparatus United Fund of China (No.U182345)National Natural Science Foundation of China (No.21471091)+3 种基金Guangdong Province Science and Technology Plan Project for Public Welfare Fund and Ability Construction Project (No.2017A010104003)Shenzhen Science and Technology Research and Development Funds (No.JCYJ20170818104441521)the Fundamental Research Funds of Shandong University (No. 2018JC022)the Taishan Scholar Project of Shandong Province (No.ts201511004).
文摘Lithium-sulfur (Li-S) battery as one of the most attractive candidates for energy storage systems has attracted extensive interests.Herein,for the first time,hierarchical flower-like cobalt phosphosulfide architectures (defined as "CoSP") derived from Prussian blue analogue (PBA) was fabricated through the conversion of Co-based PBA in PxSy atmosphere.The as-prepared polar CoSP could effectively trap polysulfides through the formation of strong chemical bonds.In addition,after the combination of CoSP with high conductive rGO,the obtained CoSP/rGO as sulfur host material exhibits ultralow capacity decay rate of 0.046% per cycle over 900 cycles at a current density of 1 C.The excellent performance could be attributed to the shortened lithium diffusion pathways,fastened electron transport ability during polysulfide conversion,and increased much more anchor active sites to polysulfides,which is expected to be a promising material for Li-S batteries.It is believed that the as-prepared CoSP/rGO architectures will shed light on the development of novel promising materials for Li-S batteries with high cycle stability.