This study explores the implementation of computed tomography(CT)reconstruction and simulation techniques for patient-specific valves,aiming to dissect the mechanical attributes of calcified valves within transcathete...This study explores the implementation of computed tomography(CT)reconstruction and simulation techniques for patient-specific valves,aiming to dissect the mechanical attributes of calcified valves within transcatheter heart valve replacement(TAVR)procedures.In order to facilitate this exploration,it derives pertinent formulas for 3D multi-material isogeometric hyperelastic analysis based on Hounsfield unit(HU)values,thereby unlocking foundational capabilities for isogeometric analysis in calcified aortic valves.A series of uniaxial and biaxial tensile tests is executed to obtain an accurate constitutive model for calcified active valves.To mitigate discretization errors,methodologies for reconstructing volumetric parametric models,integrating both geometric and material attributes,are introduced.Applying these analytical formulas,constitutive models,and precise analytical models to isogeometric analyses of calcified valves,the research ascertains their close alignment with experimental results through the close fit in displacement-stress curves,compellingly validating the accuracy and reliability of the method.This study presents a step-by-step approach to analyzing themechanical characteristics of patient-specific valves obtained fromCT images,holding significant clinical implications and assisting in the selection of treatment strategies and surgical intervention approaches in TAVR procedures.展开更多
In this paper,a new isogeometric topology optimization(ITO)method is proposed by using T-splines based isogeometric analysis(IGA).The arbitrarily shaped design domains,directly obtained from CAD,are represented by a s...In this paper,a new isogeometric topology optimization(ITO)method is proposed by using T-splines based isogeometric analysis(IGA).The arbitrarily shaped design domains,directly obtained from CAD,are represented by a single T-spline surface which overcomes the topological limitations of Non-Uniform Rational B-Spline(NURBS).The coefficients correlated with control points are directly used as design variables.Therefore,the T-spline basis functions applied for geometry description and calculation of structural response are simultaneously introduced to represent the density distribution.Several numerical examples show that the proposed approach leads to a coherent workflow to handle design problems of complicated structures.The optimized results are free of checkerboard patterns without additional stabilization and filtering techniques due to the properties of T-splines,which also simplified the post-processing.In addition,through performing local refinement,we can easily achieve multiresolution optimization and infill optimization within the T-splines based framework.In general,the proposed method provides a possibility to design,analyze,and optimize engineering structures in a uniform model,which has the potential to improve design efficiency and reduce the cost of product development.展开更多
Nonlinear behaviors are commonplace in many complex engineering applications,e.g.,metal forming,vehicle crash test and so on.This paper focuses on the T-spline based isogeometric analysis of two-dimensional nonlinear ...Nonlinear behaviors are commonplace in many complex engineering applications,e.g.,metal forming,vehicle crash test and so on.This paper focuses on the T-spline based isogeometric analysis of two-dimensional nonlinear problems including general large deformation hyperelastic problems and small deformation elastoplastic problems,to reveal the advantages of local refinement property of T-splines in describing nonlinear behavior of materials.By applying the adaptive refinement capability of T-splines during the iteration process of analysis,the numerical simulation accuracy of the nonlinear model could be increased dramatically.The Bézier extraction of the T-splines provides an element structure for isogeometric analysis that can be easily incorporated into existing nonlinear finite element codes.In addition,T-splines show great superiority of modeling complex geometries especially when the model is irregular and with hole features.Several numerical examples have been tested to validate the accuracy and convergence of the proposed method.The obtained results are compared with those from NURBS-based isogeometric analysis and commercial software ABAQUS.展开更多
To overcome the topological constraints of non-uniform rational B-splines,T-splines have been proposed to define the freeform surfaces.The introduction of T-junctions and extraordinary points makes it possible to repr...To overcome the topological constraints of non-uniform rational B-splines,T-splines have been proposed to define the freeform surfaces.The introduction of T-junctions and extraordinary points makes it possible to represent arbitrarily shaped models by a single T-spline surface.Whereas,the complexity and flexibility of topology structure bring difficulty in programming,which have caused a great obstacle for the development and application of T-spline technologies.So far,research literatures concerning T-spline data structures compatible with extraordinary points are very scarce.In this paper,an efficient data structure for calculation of unstructured T-spline surfaces is developed,by which any complex T-spline surface models can be easily and efficiently computed.Several unstructured T-spline surface models are calculated and visualized in our prototype system to verify the validity of the proposed method.展开更多
Antibody-mediated rejection(AMR)is one of the major causes of graft loss after transplantation.Recently,the regulation of B cell differentiation and the prevention of donor-specific antibody(DSA)production have gained...Antibody-mediated rejection(AMR)is one of the major causes of graft loss after transplantation.Recently,the regulation of B cell differentiation and the prevention of donor-specific antibody(DSA)production have gained increased attention in transplant research.Herein,we established a secondary allogeneic in vivo skin transplant model to study the effects of romidepsin(FK228)on DSA.The survival of grafted skins was monitored daily.The serum levels of DSA and the number of relevant immunocytes in the recipient spleens were evaluated by flow cytometry.Then,we isolated and purified B cells from B6mouse spleens in vitro by magnetic bead sorting.The B cells were cultured with interleukin-4(IL-4)and anti-clusters of differentiation 40(CD40)antibody with or without FK228 treatment.The immunoglobulin G1(IgG1)and IgM levels in the supernatant were evaluated by enzyme-linked immunosorbent assay(ELISA).Quantitative reverse transcription-polymerase chain reaction(RT-q PCR)and western blotting were conducted to determine the corresponding levels of messenger RNA(mRNA)and protein expression in cultured cells and the recipient spleens.The results showed that FK228 significantly improved the survival of allogeneic skin grafts.Moreover,FK228 inhibited DSA production in the serum along with the suppression of histone deacetylase 1(HADC1)and HDAC2 and the upregulation of the acetylation of histones H2A and H3.It also inhibited the differentiation of B cells to plasma cells,decreased the transcription of positive regulatory domain-containing 1(Prdm1)and X-box-binding protein 1(Xbp1),and decreased the expression of phosphorylated inositol-requiring enzyme 1α(p-IRE1α),XBP1,and B lymphocyte-induced maturation protein-1(Blimp-1).In conclusion,FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway.Thus,FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR.展开更多
基金supported by the Natural Science Foundation of China(Project Nos.52075340 and 61972011)the Shanghai Special Research Project on Aging Population and Maternal and Child Health(Project No.2020YJZX0106).
文摘This study explores the implementation of computed tomography(CT)reconstruction and simulation techniques for patient-specific valves,aiming to dissect the mechanical attributes of calcified valves within transcatheter heart valve replacement(TAVR)procedures.In order to facilitate this exploration,it derives pertinent formulas for 3D multi-material isogeometric hyperelastic analysis based on Hounsfield unit(HU)values,thereby unlocking foundational capabilities for isogeometric analysis in calcified aortic valves.A series of uniaxial and biaxial tensile tests is executed to obtain an accurate constitutive model for calcified active valves.To mitigate discretization errors,methodologies for reconstructing volumetric parametric models,integrating both geometric and material attributes,are introduced.Applying these analytical formulas,constitutive models,and precise analytical models to isogeometric analyses of calcified valves,the research ascertains their close alignment with experimental results through the close fit in displacement-stress curves,compellingly validating the accuracy and reliability of the method.This study presents a step-by-step approach to analyzing themechanical characteristics of patient-specific valves obtained fromCT images,holding significant clinical implications and assisting in the selection of treatment strategies and surgical intervention approaches in TAVR procedures.
基金supported by the Natural Science Foundation of China(Project Nos.61972011 and 61572056).
文摘In this paper,a new isogeometric topology optimization(ITO)method is proposed by using T-splines based isogeometric analysis(IGA).The arbitrarily shaped design domains,directly obtained from CAD,are represented by a single T-spline surface which overcomes the topological limitations of Non-Uniform Rational B-Spline(NURBS).The coefficients correlated with control points are directly used as design variables.Therefore,the T-spline basis functions applied for geometry description and calculation of structural response are simultaneously introduced to represent the density distribution.Several numerical examples show that the proposed approach leads to a coherent workflow to handle design problems of complicated structures.The optimized results are free of checkerboard patterns without additional stabilization and filtering techniques due to the properties of T-splines,which also simplified the post-processing.In addition,through performing local refinement,we can easily achieve multiresolution optimization and infill optimization within the T-splines based framework.In general,the proposed method provides a possibility to design,analyze,and optimize engineering structures in a uniform model,which has the potential to improve design efficiency and reduce the cost of product development.
基金support by the Natural Science Foundation of China(Project Nos.61972011 and 61572056).
文摘Nonlinear behaviors are commonplace in many complex engineering applications,e.g.,metal forming,vehicle crash test and so on.This paper focuses on the T-spline based isogeometric analysis of two-dimensional nonlinear problems including general large deformation hyperelastic problems and small deformation elastoplastic problems,to reveal the advantages of local refinement property of T-splines in describing nonlinear behavior of materials.By applying the adaptive refinement capability of T-splines during the iteration process of analysis,the numerical simulation accuracy of the nonlinear model could be increased dramatically.The Bézier extraction of the T-splines provides an element structure for isogeometric analysis that can be easily incorporated into existing nonlinear finite element codes.In addition,T-splines show great superiority of modeling complex geometries especially when the model is irregular and with hole features.Several numerical examples have been tested to validate the accuracy and convergence of the proposed method.The obtained results are compared with those from NURBS-based isogeometric analysis and commercial software ABAQUS.
基金The authors would like to acknowledge the support by the National Natural Science Foundation of China(Nos.61572056 and 51305016).
文摘To overcome the topological constraints of non-uniform rational B-splines,T-splines have been proposed to define the freeform surfaces.The introduction of T-junctions and extraordinary points makes it possible to represent arbitrarily shaped models by a single T-spline surface.Whereas,the complexity and flexibility of topology structure bring difficulty in programming,which have caused a great obstacle for the development and application of T-spline technologies.So far,research literatures concerning T-spline data structures compatible with extraordinary points are very scarce.In this paper,an efficient data structure for calculation of unstructured T-spline surfaces is developed,by which any complex T-spline surface models can be easily and efficiently computed.Several unstructured T-spline surface models are calculated and visualized in our prototype system to verify the validity of the proposed method.
基金supported by the National Natural Science Foundation of China(Nos.81873511 and 81471587)。
文摘Antibody-mediated rejection(AMR)is one of the major causes of graft loss after transplantation.Recently,the regulation of B cell differentiation and the prevention of donor-specific antibody(DSA)production have gained increased attention in transplant research.Herein,we established a secondary allogeneic in vivo skin transplant model to study the effects of romidepsin(FK228)on DSA.The survival of grafted skins was monitored daily.The serum levels of DSA and the number of relevant immunocytes in the recipient spleens were evaluated by flow cytometry.Then,we isolated and purified B cells from B6mouse spleens in vitro by magnetic bead sorting.The B cells were cultured with interleukin-4(IL-4)and anti-clusters of differentiation 40(CD40)antibody with or without FK228 treatment.The immunoglobulin G1(IgG1)and IgM levels in the supernatant were evaluated by enzyme-linked immunosorbent assay(ELISA).Quantitative reverse transcription-polymerase chain reaction(RT-q PCR)and western blotting were conducted to determine the corresponding levels of messenger RNA(mRNA)and protein expression in cultured cells and the recipient spleens.The results showed that FK228 significantly improved the survival of allogeneic skin grafts.Moreover,FK228 inhibited DSA production in the serum along with the suppression of histone deacetylase 1(HADC1)and HDAC2 and the upregulation of the acetylation of histones H2A and H3.It also inhibited the differentiation of B cells to plasma cells,decreased the transcription of positive regulatory domain-containing 1(Prdm1)and X-box-binding protein 1(Xbp1),and decreased the expression of phosphorylated inositol-requiring enzyme 1α(p-IRE1α),XBP1,and B lymphocyte-induced maturation protein-1(Blimp-1).In conclusion,FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway.Thus,FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR.