The real-time dynamic liquid level calculation method of the sucker rod well based on multi-view features fusion

In the production of the sucker rod well, the dynamic liquid level is important for the production efficiency and safety in the lifting process. It is influenced by multi-source data which need to be combined for the dynamic liquid level real-time calculation. In this paper, the multi-source data are regarded as the different views including the load of the sucker rod and liquid in the wellbore, the image of the dynamometer card and production dynamics parameters. These views can be fused by the multi-branch neural network with special fusion layer. With this method, the features of different views can be extracted by considering the difference of the modality and physical meaning between them. Then, the extraction results which are selected by multinomial sampling can be the input of the fusion layer.During the fusion process, the availability under different views determines whether the views are fused in the fusion layer or not. In this way, not only the correlation between the views can be considered, but also the missing data can be processed automatically. The results have shown that the load and production features fusion(the method proposed in this paper) performs best with the lowest mean absolute error(MAE) 39.63 m, followed by the features concatenation with MAE 42.47 m. They both performed better than only a single view and the lower MAE of the features fusion indicates that its generalization ability is stronger. In contrast, the image feature as a single view contributes little to the accuracy improvement after fused with other views with the highest MAE. When there is data missing in some view, compared with the features concatenation, the multi-view features fusion will not result in the unavailability of a large number of samples. When the missing rate is 10%, 30%, 50% and 80%, the method proposed in this paper can reduce MAE by 5.8, 7, 9.3 and 20.3 m respectively. In general, the multi-view features fusion method proposed in this paper can improve the accuracy obviously and process the missing data effectively, which helps provide technical support for real-time monitoring of the dynamic liquid level in oil fields.

Targeting colorectal cancer with Herba Patriniae and Coix seed:Network pharmacology,molecular docking,and in vitro validation

BACKGROUND Herba Patriniae and Coix seed(HC)constitute a widely utilized drug combination in the clinical management of colorectal cancer(CRC)that is known for its diuretic,anti-inflammatory,and swelling-reducing properties.Although its efficacy has been demonstrated in a clinical setting,the active compounds and their mechanisms of action in CRC treatment remain to be fully elucidated.AIM To identify the active,CRC-targeting components of HC and to elucidate the mechanisms of action involved.METHODS Active HC components were identified and screened using databases.Targets for each component were predicted.CRC-related targets were obtained from human gene databases.Interaction targets between HC and CRC were identified.A“drug-ingredient-target”network was created to identify the core components and targets involved.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were conducted to elucidate the key pathways involved.Molecular docking between core targets and key components was executed.In vitro experiments validated core monomers.RESULTS Nineteen active components of HC were identified,with acacetin as the primary active compound.The predictive analysis identified 454 targets of the active compounds in HC.Intersection mapping with 2685 CRC-related targets yielded 171 intervention targets,including 30 core targets.GO and KEGG analyses indicated that HC may influence the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway.Molecular docking showed that acacetin exhibited an optimal interaction with AKT1,identifying PI3K,AKT,and P53 as key genes likely targeted by HC during CRC treatment.Acacetin inhibited HT-29 cell proliferation and migration,as well as promoted apoptosis,in vitro.Western blotting analysis revealed increased p53 and cleaved caspase-3 expression and decreased levels of p-PI3K,p-Akt,and survivin,which likely contributed to CRC apoptosis.CONCLUSION Acacetin,the principal active compound in the HC pair,inhibited the proliferation and migration of HT-29 cells and promoted apoptosis through the PI3K/Akt/p53 signaling pathway.

Minimalistic approach to left atrial appendage occlusion guided by cardiac computed tomography angiography

OBJECTIVE To assess the feasibility and safety of the minimalistic approach to left atrial appendage occlusion(LAAO) guided by cardiac computed tomography angiography(CCTA).METHODS Ninety consecutive patients who underwent LAAO, with or without CCTA-guided, were matched(1:2). Each step of the LAAO procedure in the computed tomography(CT) guidance group(CT group) was directed by preprocedural CT planning. In the control group, LAAO was performed using the standard method. All patients were followed up for 12 months, and device surveillance was conducted using CCTA.RESULTS A total of 90 patients were included in the analysis, with 30 patients in the CT group and 60 matched patients in the control group. All patients were successfully implanted with Watchman devices. The mean ages for the CT group and the control group were 70.0 ± 9.4 years and 68.4 ± 11.9 years(P = 0.52), respectively. The procedure duration(45.6 ± 10.7 min vs. 58.8 ± 13.0 min,P < 0.001) and hospital stay(7.5 ± 2.4 day vs. 9.6 ± 2.8 day, P = 0.001) in the CT group was significantly shorter compared to the control group. However, the total radiation dose was higher in the CT group compared to the control group(904.9 ± 348.0 m Gy vs.711.9 ± 211.2 m Gy, P = 0.002). There were no significant differences in periprocedural pericardial effusion(3.3% vs. 6.3%, P = 0.8) between the two groups. The rate of postprocedural adverse events(13.3% vs. 18.3%, P = 0.55) were comparable between both groups at 12 months follow-up.CONCLUSIONS CCTA is capable of detailed LAAO procedure planning. Minimalistic LAAO with preprocedural CCTA planning was feasible and safe, with shortened procedure time and acceptable increased radiation and contras consumption. For patients with contraindications to general anesthesia and/or transesophageal echocardiography, this promising method may be an alternative to conventional LAAO.

Hysteroscopic cervical biopsy for women with persistent human papillomavirus infection after loop electrosurgical excision procedure: A case report

BACKGROUNDAlmost all cases of cervical cancer can be attributed to human papillomavirus(HPV) infection. The loop electrosurgical excision procedure (LEEP) is widelyused to treat HPV-mediated disease;thus, cervical cancer is highly preventable.However, LEEP does not necessarily clear HPV rapidly and may affect theaccuracy of the results of ThinPrep cytology test (TCT) and cervical biopsy due tothe formation of cervical scars.CASE SUMMARYA 40-year-old woman underwent LEEP for cervical intraepithelial neoplasia grade1 approximately 10 years ago. Subsequent standard cervical cancer screeningsuggested persistent HPV-52 infection, but TCT results were negative. Cervicalbiopsy under colposcopy was performed thrice over a 10-year period, yieldingnegative pathology results. She developed abnormal vaginal bleeding after sexualactivity, persisting for approximately 1 year, and underwent hysteroscopy in ourhospital. Histopathologic evaluation confirmed adenocarcinoma in situ of theuterine cervix.CONCLUSIONPatients with long-term persistent, high-risk HPV infection and negative pathologyresults of cervical biopsy after LEEP are at risk of cervical cancer. Hysteroscopicresection of cervical canal tissue is recommended as a supplement tocervical biopsy because it helps define the lesion site and may yield a pathologicdiagnosis.

自噬参与肝纤维化的机制

自噬是细胞依赖溶酶体的一种代谢过程,通过分解细胞内蛋白质、脂质等成分,循环再利用分解产物,使细胞适应不利的生活环境,是机体维持内环境稳态的重要机制.肝纤维化是指肝组织内细胞外基质成分过度增生与异常沉积,导致肝脏结构和功能异常的病理变化,肝纤维化见于多数不同病因的慢性肝脏疾病中.本文总结了自噬参与肝纤维化的作用以及与肝纤维化相关的自噬信号通路,进一步阐述细胞自噬参与肝纤维化的机制.

Beneficial effects of moderate to vigorous physical activity on cardiovascular disease among Chinese adults

Background In China,lack of evidence remains a significant challenge for the national initiative to promote physical activity(PA).We aimed to quantify the beneficial effects of meeting or maintaining the recommended PA level[150 minutes per week(min/wk)of moderate PA or 75 min/wk of vigorous PA or an equivalent combination]on incident cardiovascular disease(CVD)among Chinese population.Methods We included 100,560 participants without history of CVD from three cohorts in the Prediction for Atherosclerotic Cardiovascular Disease Risk in China(China-PAR)project.Cox proportional hazards models were used to estimate hazard ratios(HRs)and 95%confidence intervals(CIs)for CVD events and its subtypes,including stroke,coronary heart disease,heart failure,and CVD death.Results During a median follow-up of 7.3 years(range:6–15 years),777,163 person-years and 4693 incident CVD events were observed.Compared with participants who were inactive at baseline,the multivariable adjusted HR(95%CI)of developing CVD was 0.74(0.69–0.79)for those who met recommended moderate to vigorous physical activity(MVPA)level at baseline.Furthermore,the risk of CVD incidence was reduced with increment of MVPA(Ptrend<0.001),and the HR(95%CI)of highly-active versus inactive category was 0.62(0.56–0.68).Compared with individuals who were inactive both at the baseline and follow-up,those keeping active over the period of follow-up had a substantial lower risk of incident CVD with the HR(95%CI)of 0.57(0.43–0.77).Conclusions The findings demonstrated that meeting and maintaining the recommended MVPA level could reduce the cardiovascular risk.Wider adoption of the PA recommendations would have considerable health impacts to the Chinese population.

Primary analysis and screening of microRNAs in gastric cancer side population cells

AIM: To explore the micro RNA(mi RNA) profiles and to determine the key mi RNAs within the side population(SP) cells of the gastric cancer cell line MKN-45. METHODS: We used fluorescence-activated cell sorting and Hoechst 33342 labeling to obtain SP cells from the human gastric carcinoma cell line MKN-45. The mi RNA expression profiles of the SP and major population(MP) cells were examined using a mi RNA gene chip, and key mi RNAs were obtained according to aberrant expression and the mi RNAs' possible targets as predicted by bioinformatics. RESULTS: Using a significance criterion of a 1.5-fold or greater difference in expression level, we observed an increase in the expression of 34 mi RNAs and a decrease in the expression of 34 mi RNAs when comparing SP to MP cells. Using quantitative real-time reverse transcription-polymerase chain reaction to test for differentially expressed mi RNAs combined with bioinformatics results, we found that the downregulated mi RNAs, such as hsa-mi R-3175 and hsa-mi R-203, and the upregulated mi RNAs, including hsa-mi R-130 a, hsa-mi R-324-5p, hsa-mi R-34 a, and hsa-mi R-25-star, may be important in maintaining and regulating the characteristics of SP cells. CONCLUSION: There are key mi RNAs expressed within the SP cells of the gastric cancer cell line MKN-45, andinclude hsa-mi R-3175, hsa-mi R-203, hsa-mi R-130 a, hsami R-324-5p, hsa-mi R-34 a, and hsa-mi R-25-star.

Associations of soybean products intake with blood pressure changes and hypertension incidence: the China-PAR project

Background The relationships between dietary intake of soybean products and incident hypertension were still uncertain.This study aimed to illustrate the associations between intake of soybean products with risks of incident hypertension and longitudinal changes of blood pressure in a prospective cohort study.Methods We included 67,499 general Chinese adults from the Project of Prediction for Atherosclerosis Cardiovascular Disease Risk in China(China-PAR).Information about soybean products consumption was collected by standardized questionnaires,and study participants were categorized into the ideal(≥125 g/day)or non-ideal(<125 g/day)group.Hazard ratios(HRs)and corresponding 95%confidence intervals(95%CIs)for incident hypertension were calculated using Cox proportional hazard models.Among participants with repeated measures of blood pressure,generalized linear models were used to examine the relationships between soybean products consumption and blood pressure changes.Results During a median follow-up of 7.4 years,compared with participants who consumed<125 g of soybean products per day,multivariable adjusted HR for those in the ideal group was 0.73(0.67-0.80).This inverse association remained robust across most subgroups while significant interactions were tested between soybean products intake and age,sex,urbanization and geographic region(P values for interaction<0.05).The mean systolic and diastolic blood pressure levels were 1.05(0.71-1.39)mmHg and 0.44(0.22-0.66)mmHg lower among participants in the ideal group than those in the non-ideal group.Conclusions Our study showed that intake of soybean products might reduce the long-term blood pressure levels and hypertension incidence among Chinese population,which has important public health implications for primary prevention of hypertension.

Non-surgical contraindication for acute appendicitis with secondary thrombocytopenia: A case report

A 26-year-old man presented with migrated right lower abdominal pain and without any history of hematological systemic diseases.Blood routine test showed a leukocyte count of 22.74 × 109/L, with91.4% neutrophils, and a platelet count of 4 × 109/L before admission.The case question was whether the team should proceed with surgery.Obviously, a differential diagnosis is essential before making such a decision.Acute appendicitis was easily diagnosed based on clinical findings, including migrating abdominal pain, a leukocyte count of 22.74 × 109/L and the result of abdominal computed tomography scan.However, it was not clear whether the severe thrombocytopenia was primary or secondary.So smear of peripheral blood and aspiration of bone marrow were ordered to exclude hematological diseases.Neither of the tests indicated obvious pathological hematological changes.There was no hepatosplenomegaly found by ultrasound examination of the liver and spleen.Therefore, operative intervention may be a unique clinical scenario in acute severe appendicitis patients with secondary thrombocytopenia.

IDENTIFICATION OF DIFFERENTIAL GENES IN OVARIAN CANCER USING REPRESENTATIONAL DIFFERENCE ANALYSIS OF cDNA

Objoctive To identify differential genes between normal ovarian epithelium tissue and ovarian epithelial cancer using representational difference analysis of cDNA （cDNA-RDA）. Methods cDNA-RDA was performed to identify the differentially expressed sequences between cDNAs from cancer tissue and cDNAs from normal ovarian tissue in the same patient who was in the early stage of ovarian serous cystadenocarcinoma. These differentially expressed fragments were cloned and analyzed, then sequenced and compared with known genes. Results Three differentially cxpressed cDNA fragments were isolated using cDNA from normal ovarian tissue as tester and cDNA from cancer tissue as driver amplicon by cDNA-RDA. DP Ⅲ- 1 and DP Ⅲ-2 cDNA clone showed significant homology to the cDNA of alpha actin gene; DPⅢ-3 cDNA clone showed significant homology to the cDNA oftransgelin gene. Conclusion cDNA-RDA can bc used to sensitively identify the differentially expressed genes in ovarian serous cystadenocarcinoma. Ovarian serous cystadenocarcinoma involves alteration of multiple genes.

Intraorbital wooden foreign bodies: case series and literature review

Intraorbital wooden foreign bodies(IOWFBs) constitute a relatively rare ocular trauma, which occupy a special type of intraorbital foreign bodies(IOFBs). Data regarding IOWFBs must be obtained from case reports or small case series due to their rarity. Here, we reported 5 cases of IOWFBs and reviewed the related literatures, which could provide comprehensive information regarding the clinical manifestations, diagnosis, and surgical treatment of IOWFBs. Combined with the published literature, a total of 51 independent cases were counted after we added 5 cases. Among them, the number of male and female patients was 35 and 16 respectively;the mean age was 27.3±18.2(range 1-66)y. Obviously, the disorder seemed to occur mainly in young and middle-aged people. Because of the diversity in the clinical manifestations and imaging characteristics of IOWFBs, misdiagnosis and missed diagnosis often occur during the initial visit. Delayed diagnosis may lead to a high risk of orbital infection caused by IOWFBs. Surgery is the treatment of choice for most patients;however, the missed diagnosis and residue of foreign bodies after previous surgery cannot be ignored. Therefore, an accurate diagnosis is governed by the detailed trauma history, careful ocular examination, close observation of clinical manifestations, correct imaging diagnosis [e.g., magnetic resonance imaging(MRI) or computerized tomography(CT)], and timely and completely elimination of IOWFBs.

Study on the mechanism of"mulberry twig-cassia twig"in the treatment of rheumatoid arthritis based on network pharmacology

Objective:To explore the main active components,main target genes and key pathways of"mulberry twig-cassia twig"in the treatment of rheumatoid arthritis based on network pharmacology Methods:The active components and targets of drug pairs were screened by TCMSP database and Swisstargetprediction database,and the target genes of rheumatoid arthritis were obtained by GeneCards and OMIM database,and the overlapping genes were obtained,that is,the predictive target genes of"mulberry twig-cassia twig"medicine for the treatment of rheumatoid arthritis.The"drug-active ingredient-target"network and"drug-active ingredient-target-disease"network were constructed by Cytoscape 3.7.2 software.The PPI network was constructed by String platform and the key biological processes were analyzed and screened by Cytoscape 3.7.2 software.The GO analysis and KEGG pathway analysis of predictive target genes were carried out on the Metascape website,and the bubble diagram was drawn by Omicshare platform.At the same time,those genes can used to construct the"drug-component-target-pathway"network by Cytoscape 3.7.2 software.Results:A total of 12 active components,85 predictive targets,126 KEGG pathways and 1537 GO functions were obtained.Conclusion:The main active components of"mulberry twig-cassia twig"in the treatment of rheumatoid arthritis are kaempferol,beta-sitosterol and cinnamic acid,and the main target genes may be HSP90AB1、RELA,AKT1,TNF,TLR4,PTGS2 and so on.The main KEGG pathways are TNF signal pathway,estrogen pathway,HIF-1 signal pathway,NF-κB signal pathway,PI3K-Akt signal pathway and so on.The main GO function analysis is the response to lipopolysaccharide、response to oxidative stress、cytokine-mediated signaling pathway.This study shows that the"mulberry twig-cassia twig"drug pair is through the regulation of multip-compound,multi-target,multi-pathway to treat rheumatoid arthritis,providing a theoretical basis for the follow-up study of traditional Chinese medicine in the treatment of rheumatoid arthritis.

Improved Performance in Differentiating Benign from Malignant Sinonasal Tumors Using Diffusion-weighted Combined with Dynamic Contrast-enhanced Magnetic Resonance Imaging

Background：Differentiating benign from malignant sinonsal lesions is essential for treatment planning as well as determining the patient＇s prognosis,but the differentiation is often difficult in clinical practice.The study aimed to determine whether the combination of diffusion-weighted （DW） and dynamic contrast-enhanced magnetic resonance imaging （DCE-MRI） can improve the performance in differentiating benign from malignant sinonasal tumors.Methods：This retrospective study included 197 consecutive patients with sinonasal tumors （116 malignant tumors and 81 benign tumors）.All patients underwent both DW and DCE-MRI in a 3-T magnetic resonance scanner.Two different settings ofb values （0,700 and 0,1000 s/mm^2） and two different strategies of region of interest （ROI） including whole slice （WS） and partial slice （PS） were used to calculate apparent diffusion coefficients （ADCs）.A DW parameter with WS ADCsb0.1000 and two DCE-MRI parameters （time intensity curve [TIC] and time to peak enhancement [Tpeak]） were finally combined to use in differentiating the benign from the malignant tumors in this study.Results：The mean ADCs of malignant sinonasal tumors （WS ADCsb0,1000=1.084 × 10^-3 mm^2/s） were significantly lower than those of benign tumors （WS ADCsb0,1000=1.617 × 10^-3 mm^2/s,P 〈 0.001）.The accuracy using WS ADCsb0,1000 alone was 83.7% in differentiating the benign from the malignant tumors （85.3% sensitivity,81.2% specificity,86.4% positive predictive value [PPV],and 79.5% negative predictive value [NPV]）.The accuracy using DCE with Tpeak and TIC alone was 72.1% （69.1% sensitivity,74.1% specificity,77.5% PPV,and 65.1% NPV）.Using DW-MRI parameter was superior than using DCE parameters in differentiation between benign and malignant sinonasal tumors （P 〈 0.001）.The accuracy was 87.3% （90.5% sensitivity,82.7% specificity,88.2% PPV,and 85.9% NPV） using DW-MRI combined with DCE-MRI,which was superior than that using DCE-MRI alone or using DW-MRI alone （both P 〈 0.001） in differentiating the benign from the malignant tumors.Conclusions：Diffusion-weighted combined with DCE-MRI can improve imaging performance in differentiating benign from malignant sinonasal tumors,which has the potential to improve diagnostic accuracy and to provide added value in the management for these tumors.

Association of cardiovascular diseases with milk intake among general Chinese adults

Background:The association of milk intake with cardiovascular disease(CVD)and cause-specific mortality remained controversial and evidence among the Chinese population was limited.We aimed to study the relationship between milk intake and CVDs among general Chinese adults.Methods::A total of 104,957 participants received questionnaire survey.Results of physical examination such as anthropometric measurements and biochemical tests during 2007 to 2008,demographic data and their information on milk intake were collected through standardized questionnaires.Cox proportional hazard regression models were used to calculate hazard ratios(HRs)and their corresponding 95%confidence intervals(CIs)of CVD incidence,cause-specific mortality and all-cause mortality related to milk intake.Restricted cubic splines(RCSs)were applied to examine dose-response associations.Results::Among the 91,757 participants with a median follow-up period of 5.8 years,we documented 3877 CVD cases and 4091 all-cause deaths.Compared with participants who never consumed milk,the multivariate-adjusted HRs(95%CIs)of CVD incidence for 1 to 150 g/day,151 to 299 g/day,and≥300 g/day were 0.94(0.86-1.03)(P>0.05),0.77(0.66-0.89)(P<0.05),and 0.59(0.40-0.89)(P<0.05),respectively;each 100 g increase of daily milk intake was associated with 11%lower risk of CVD incidence(HR,0.89;95%CI:0.85-0.94;P<0.001),and 11%lower risk of CVD mortality(HR,0.89;95%CI:0.82-0.97;P=0.008)after adjustment for age,sex,residential area,geographic region,education level,family history of CVD,smoking,alcohol drinking,physical activity level,body mass index,and healthy diet status(ideal or not).RCS analyses also showed a linear dose-response relationship with CVD(P for overall significance of the curve<0.001;P for non-linearity=0.979;P for linearity<0.001)and stroke(P for overall significance of the curve=0.010;P for non-linearity=0.998;P for linearity=0.002)incidence,and CVD mortality(P for overall significance of the curve=0.045;P for non-linearity=0.768;P for linearity=0.014)within the current range of daily milk intake.Conclusions::Daily milk intake was associated with lower risk of CVD incidence and mortality in a linear inverse relationship.The findings provide new evidence for dietary recommendations in CVD prevention among Chinese adults and people with similar dietary pattern in other countries.

pH-Responsive Magnetic I-Motif Container Coupled with DNA Walker for Construction of Dual-Signal Electrochemical Biosensor

Herein,a dual-signal electrochemical biosensor has been developed by self-assembly of pH-activatable i-motif probes on magnetic microparticles(MMPs)coupled with DNA walker for signal amplification.In this study,the cytosine(C)-rich single-stranded DNAs are hybridized with DNA initiators to obtain the long-nicked duplexes with repeated units,which are further captured on MMPs to form the magnetic i-motif containers.The resulting duplexes contain abundant G-C base pairs,thus providing extensive binding sites for doxorubicin(DOX).At acidic pH,the C-rich sequences are folded into i-motif structure,resulting in the release of DOX and walker initiators.In this case,the liberated DOX is adsorbed on gra-phene quantum dots-modified glassy carbon electrode viaπ-πinteraction,while the walker initiators as a moving part can catalyze the hybridization between MB-modified fueler DNA and tracker DNA on electrode,contributing to the generation of dual electrochemical signals induced by MB and DOX.Importantly,the magnetic separation can effectively reduce the background,achieving sensitive biosensing of pH ranging from 4.0 to 7.4 with excellent stability.Moreover,the proposed dual-signal electrochemical biosensor has been successfully applied for accurate monitoring of pH in human serum,which holds great potential in pH-dependent bioassays,especially in ultra-micro analysis for clinical applications.

Validation and Refinement of Two Interpretable Models for Coronavirus Disease 2019 Prognosis Prediction

Objective:To validate two proposed coronavirus disease 2019(COVID-19)prognosis models,analyze the characteristics of different models,consider the performance of models in predicting different outcomes,and provide new insights into the development and use of artificial intelligence(AI)predictive models in clinical decision-making for COVID-19 and other diseases.Materials and Methods:We compared two proposed prediction models for COVID-19 prognosis that use a decision tree and logistic regression modeling.We evaluated the effectiveness of different model-building strategies using laboratory tests and/or clinical record data,their sensitivity and robustness to the timings of records used and the presence of missing data,and their predictive performance and capabilities in single-site and multicenter settings.Results:The predictive accuracies of the two models after retraining were improved to 93.2% and 93.9%,compared with that of the models directly used,with accuracies of 84.3% and 87.9%,indicating that the prediction models could not be used directly and require retraining based on actual data.In addition,based on the prediction model,new features obtained by model comparison and literature evidence were transferred to integrate the new models with better performance.Conclusions:Comparing the characteristics and differences of datasets used in model training,effective model verification,and a fusion of models is necessary in improving the performance of AI models.