Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A(Sema3A), expressed by sensory nerves, in mechanical loads-induced bo...Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A(Sema3A), expressed by sensory nerves, in mechanical loads-induced bone formation and nerve withdrawal using orthodontic tooth movement(OTM) model. Firstly, bone formation was activated after the 3rd day of OTM,coinciding with a decrease in sensory nerves and an increase in pain threshold. Sema3A, rather than nerve growth factor(NGF),highly expressed in both trigeminal ganglion and the axons of periodontal ligament following the 3rd day of OTM. Moreover, in vitro mechanical loads upregulated Sema3A in neurons instead of in human periodontal ligament cells(hPDLCs) within 24 hours.Furthermore, exogenous Sema3A restored the suppressed alveolar bone formation and the osteogenic differentiation of hPDLCs induced by mechanical overload. Mechanistically, Sema3A prevented overstretching of F-actin induced by mechanical overload through ROCK2 pathway, maintaining mitochondrial dynamics as mitochondrial fusion. Therefore, Sema3A exhibits dual therapeutic effects in mechanical loads-induced bone formation, both as a pain-sensitive analgesic and a positive regulator for bone formation.展开更多
A novel structure of axial flux stator and rotor dual PMs Vernier machine is presented as an effective option for high torque-density direct-driven applications.The key is to locate PMs on both sides of rotor and stat...A novel structure of axial flux stator and rotor dual PMs Vernier machine is presented as an effective option for high torque-density direct-driven applications.The key is to locate PMs on both sides of rotor and stator.With dual PMs,this proposed machine with a novel structure generates an improved torque per armature current at low speed.It is very suitable for direct drive applications with limited space such as the in-wheel electric vehicle(EV)motors.The theory of the structure with PMs on both sides is analyzed theoretically.The structure and operation principle of machine are introduced and the steady and steady performance of machine is analyzed and verified with time-stepping finite element method(TS-FEM).展开更多
By means of electroless copper deposition method a complex image has been fabricated in a mixture of cupric sulphate and formaldehyde, the surface and subsurface properties of which has been studied in detail by X-ray...By means of electroless copper deposition method a complex image has been fabricated in a mixture of cupric sulphate and formaldehyde, the surface and subsurface properties of which has been studied in detail by X-ray photoelectron spectroscopy (XPS) combined with sputter depth profiling technique analyzing distribution and chemical state of copper and silver. Depth profiling by XPS in conjunction with Ar+ sputtering shows that the catalytic activity of silver persists, catalyzing reduction of copper. The integral areas of spectra Ag3d after electroless copper deposition for 5 min at different sputtering times demonstrate that the amount of silver at the surface is greater than that in the interior. And then, the quite likely reasonable explanations are provided for the result. Additionally, the chemical shift of Ag3d XPS and deconvolution of Ag3d XPS spectrum have been also analyzed respectively at length.展开更多
Previous studies suggest that the reduction of SMAD3(mothers against decapentaplegic homolog 3)has a great impact on tumor development,but its exact pathological function remains unclear.In this study,we found that th...Previous studies suggest that the reduction of SMAD3(mothers against decapentaplegic homolog 3)has a great impact on tumor development,but its exact pathological function remains unclear.In this study,we found that the protein level of SMAD3 was greatly reduced in human-grade IV glioblastoma tissues,in which LAMP2A(lysosome-associated membrane protein type 2A)was significantly up-regulated.LAMP2A is a key ratelimiting protein of chaperone-mediated autophagy(CMA),a lysosome pathway of protein degradation that is activated in glioma.We carefully analyzed the amino-acid sequence of SMAD3 and found that it contained a pentapeptide motif biochemically related to KFERQ,which has been proposed to be a targeting sequence for CMA.In vitro,we confirmed that SMAD3 was degraded in either serum-free or KFERQ motif deleted condition,which was regulated by LAMP2A and interacted with HSC70(heat shock cognate 71 kDa protein).Using isolated lysosomes,amino-acid residues 75 and 128 of SMAD3 were found to be of importance for this process,which affected the CMA pathway in which SMAD3 was involved.Similarly,down-regulating SMAD3 or up-regulating LAMP2A in cultured glioma cells enhanced their proliferation and invasion.Taken together,these results suggest that excessive activation of CMA regulates glioma cell growth by promoting the degradation of SMAD3.Therefore,targeting the SMAD3-LAMP2Amediated CMA-lysosome pathway may be a promising approach in anti-cancer therapy.展开更多
A combined study of magnetic fabrics, zircon U-Pb geochronology and structural deformation was carried out for Late Paleozoic sedimentary and Mesozoic magmatic rocks in the southwestern Fujian rift basin, South China,...A combined study of magnetic fabrics, zircon U-Pb geochronology and structural deformation was carried out for Late Paleozoic sedimentary and Mesozoic magmatic rocks in the southwestern Fujian rift basin, South China, aiming at deciphering the tectonic evolution during Late Mesozoic. Field observations showed that the Late Mesozoic structure deformations in southwestern Fujian were categorized into four phases： NW-SE compression, ENE-WSW extension, NNE-SSW compression and NNW-SSE extension, se- quentially. Zircons picked out from Juzhou granite and WNW-trending diabase dykes showed complete crys- tal shapes and clear oscillatory zonings on their edges, and the U-Pb dating yielded ages of 132 and 141 Ma, respectively. The susceptibility ellipsoid magnitude parameters of the Juzhou granite are characterized by flaser type strain ellipsoid, with pole density center of K3 falling into the first and the third quadrants, these fea- tures revealed that the Juzhou granite formed in ENE-WSW compressional stress field, indicating the early stage of Early Cretaceous extrusion in southwestern Fujian. The late stage of Early Cretaceous NNE-SSW ex- tension was limited by the widespread WNW-trending diabase dykes, which were usually regarded as impor- tant indications for a regional extensional setting. On the basic of the previous researches, structural deforma- tion studies, and the deductions above, it can be concluded that southwestern Fujian experienced five main tectonic stages during Late Mesozoic： Early Jurassic extension, Middie-Late Jurassic thrusting, early stage of Early Cretaceous extension, late stage of Early Cretaceous compression and Late Cretaceous extension.展开更多
A novel structure of Veriner permanent magnet(VPM)machine is presented as an effective option for high torque density direct-driven applications.The key is to locate PMs on both sides of rotor and stator,and design th...A novel structure of Veriner permanent magnet(VPM)machine is presented as an effective option for high torque density direct-driven applications.The key is to locate PMs on both sides of rotor and stator,and design the stator PMs as a cylinder shape,the rotation of which can be controlled by an external driven system.With this design,magnetic field in the airgap can be directly regulated by the rotation of stator PMs.The structure and operation principle are introduced and the performance is analyzed with time-stepping finite element method(FEM).Analysis results show that the novel structure has really high torque density and good field weakening performance.展开更多
基金supported in part by National Natural Science Foundation of China(32271364 & 31971240)Interdisciplinary innovation project from West China Hospital of Stomatology, Sichuan University(RD-03-202305)。
文摘Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A(Sema3A), expressed by sensory nerves, in mechanical loads-induced bone formation and nerve withdrawal using orthodontic tooth movement(OTM) model. Firstly, bone formation was activated after the 3rd day of OTM,coinciding with a decrease in sensory nerves and an increase in pain threshold. Sema3A, rather than nerve growth factor(NGF),highly expressed in both trigeminal ganglion and the axons of periodontal ligament following the 3rd day of OTM. Moreover, in vitro mechanical loads upregulated Sema3A in neurons instead of in human periodontal ligament cells(hPDLCs) within 24 hours.Furthermore, exogenous Sema3A restored the suppressed alveolar bone formation and the osteogenic differentiation of hPDLCs induced by mechanical overload. Mechanistically, Sema3A prevented overstretching of F-actin induced by mechanical overload through ROCK2 pathway, maintaining mitochondrial dynamics as mitochondrial fusion. Therefore, Sema3A exhibits dual therapeutic effects in mechanical loads-induced bone formation, both as a pain-sensitive analgesic and a positive regulator for bone formation.
基金This work was supported in part by the Research Grant Council,the Hong Kong Special Administrative Region,China,under projects PolyU 152130/14E.
文摘A novel structure of axial flux stator and rotor dual PMs Vernier machine is presented as an effective option for high torque-density direct-driven applications.The key is to locate PMs on both sides of rotor and stator.With dual PMs,this proposed machine with a novel structure generates an improved torque per armature current at low speed.It is very suitable for direct drive applications with limited space such as the in-wheel electric vehicle(EV)motors.The theory of the structure with PMs on both sides is analyzed theoretically.The structure and operation principle of machine are introduced and the steady and steady performance of machine is analyzed and verified with time-stepping finite element method(TS-FEM).
文摘By means of electroless copper deposition method a complex image has been fabricated in a mixture of cupric sulphate and formaldehyde, the surface and subsurface properties of which has been studied in detail by X-ray photoelectron spectroscopy (XPS) combined with sputter depth profiling technique analyzing distribution and chemical state of copper and silver. Depth profiling by XPS in conjunction with Ar+ sputtering shows that the catalytic activity of silver persists, catalyzing reduction of copper. The integral areas of spectra Ag3d after electroless copper deposition for 5 min at different sputtering times demonstrate that the amount of silver at the surface is greater than that in the interior. And then, the quite likely reasonable explanations are provided for the result. Additionally, the chemical shift of Ag3d XPS and deconvolution of Ag3d XPS spectrum have been also analyzed respectively at length.
基金supported by the National Key R&D Program of China(2016YFC1306601 and 2017YFC1306002)the National Natural Science Foundation of China(82071416,81870992,81870856,U1603281,81903958,81901282,and 82004459)+3 种基金the Natural Science Foundation of Guangdong Province(2020A1515010986,2019A1515011189,and 2018A030310521)a Science and Technol-ogy Planning Project of Guangdong Province(A2018315)a Tech-nology Project of Guangzhou(2018-1202-SF-0019 and 2019ZD09)and a China Postdoctoral Science Foundation Grant(2019M662873)。
文摘Previous studies suggest that the reduction of SMAD3(mothers against decapentaplegic homolog 3)has a great impact on tumor development,but its exact pathological function remains unclear.In this study,we found that the protein level of SMAD3 was greatly reduced in human-grade IV glioblastoma tissues,in which LAMP2A(lysosome-associated membrane protein type 2A)was significantly up-regulated.LAMP2A is a key ratelimiting protein of chaperone-mediated autophagy(CMA),a lysosome pathway of protein degradation that is activated in glioma.We carefully analyzed the amino-acid sequence of SMAD3 and found that it contained a pentapeptide motif biochemically related to KFERQ,which has been proposed to be a targeting sequence for CMA.In vitro,we confirmed that SMAD3 was degraded in either serum-free or KFERQ motif deleted condition,which was regulated by LAMP2A and interacted with HSC70(heat shock cognate 71 kDa protein).Using isolated lysosomes,amino-acid residues 75 and 128 of SMAD3 were found to be of importance for this process,which affected the CMA pathway in which SMAD3 was involved.Similarly,down-regulating SMAD3 or up-regulating LAMP2A in cultured glioma cells enhanced their proliferation and invasion.Taken together,these results suggest that excessive activation of CMA regulates glioma cell growth by promoting the degradation of SMAD3.Therefore,targeting the SMAD3-LAMP2Amediated CMA-lysosome pathway may be a promising approach in anti-cancer therapy.
基金supported by the projects the China Geological Survey(Nos.12120113089600,12120114028701 and 1212011085472)the Key Project of Natural Science Foundation of China(No.41530321)the Fundamental Research Funds for the Central University(No.2652017259)
文摘A combined study of magnetic fabrics, zircon U-Pb geochronology and structural deformation was carried out for Late Paleozoic sedimentary and Mesozoic magmatic rocks in the southwestern Fujian rift basin, South China, aiming at deciphering the tectonic evolution during Late Mesozoic. Field observations showed that the Late Mesozoic structure deformations in southwestern Fujian were categorized into four phases： NW-SE compression, ENE-WSW extension, NNE-SSW compression and NNW-SSE extension, se- quentially. Zircons picked out from Juzhou granite and WNW-trending diabase dykes showed complete crys- tal shapes and clear oscillatory zonings on their edges, and the U-Pb dating yielded ages of 132 and 141 Ma, respectively. The susceptibility ellipsoid magnitude parameters of the Juzhou granite are characterized by flaser type strain ellipsoid, with pole density center of K3 falling into the first and the third quadrants, these fea- tures revealed that the Juzhou granite formed in ENE-WSW compressional stress field, indicating the early stage of Early Cretaceous extrusion in southwestern Fujian. The late stage of Early Cretaceous NNE-SSW ex- tension was limited by the widespread WNW-trending diabase dykes, which were usually regarded as impor- tant indications for a regional extensional setting. On the basic of the previous researches, structural deforma- tion studies, and the deductions above, it can be concluded that southwestern Fujian experienced five main tectonic stages during Late Mesozoic： Early Jurassic extension, Middie-Late Jurassic thrusting, early stage of Early Cretaceous extension, late stage of Early Cretaceous compression and Late Cretaceous extension.
文摘A novel structure of Veriner permanent magnet(VPM)machine is presented as an effective option for high torque density direct-driven applications.The key is to locate PMs on both sides of rotor and stator,and design the stator PMs as a cylinder shape,the rotation of which can be controlled by an external driven system.With this design,magnetic field in the airgap can be directly regulated by the rotation of stator PMs.The structure and operation principle are introduced and the performance is analyzed with time-stepping finite element method(FEM).Analysis results show that the novel structure has really high torque density and good field weakening performance.
