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Effects of anaerobic composting on tetracycline degradation in swine manure 被引量:6
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作者 Yixuan Chu Chengran Fang +3 位作者 Hua Wang xinkai wu Yijie Gu Ji Shu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2017年第10期1505-1511,共7页
Oxytetracycline(OTC) and tetracycline(TC) are the most common TC antibiotics used in human and veterinary medicine.Residual amounts of these antibiotics in manure pose a potential threat to public and ecological healt... Oxytetracycline(OTC) and tetracycline(TC) are the most common TC antibiotics used in human and veterinary medicine.Residual amounts of these antibiotics in manure pose a potential threat to public and ecological health as a result of the potential for them to be released to the environment following land application of manure from animals treated with antibiotics.We investigated the degradation of OTC and TC during anaerobic composting.We tested the effects of temperature and antibiotic concentration on degradation rates in a control and in manure spiked with TCs.We examined changes in p H,biological degradation material(BDM),and moisture corresponding with antibiotic degradation of TCs in the swine manure.Results showed that the OTC and TC concentrations decreased by between 68.54% and 95.50% in all nine treatments following 14 days of anaerobic composting,and the highest removal ef ficiencies were observed at an incubation temperature of 55°C and initial concentrations of 10.36 μg·g^(-1),and 5.96 μg·g^(-1) of OTC and TC,respectively,which were degraded by 95.50%,and 90.06%.During composting at 55 °C and at added concentrations of 5 μg·g^(-1),OTC decreased rapidly,and the time required for50% and 90% degradation was 4.1 and 9.8 days,respectively;for TC,these values were 4.4 and 14.0 days,respectively.Removal ef ficiencies for all TCs correlated well with moisture content of the manure.These results show that composting may be a practical and useful means to reduce concentrations of OTC and TC in swine manure prior to its land application. 展开更多
关键词 Antibiotics Tetracyclines Anaerobic composting Swine manure
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Robust Damage Detection and Localization Under Complex Environmental Conditions Using Singular Value Decomposition-based Feature Extraction and One-dimensional Convolutional Neural Network
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作者 Shengkang Zong Sheng Wang +3 位作者 Zhitao Luo xinkai wu Hui Zhang Zhonghua Ni 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2023年第3期252-261,共10页
Ultrasonic guided wave is an attractive monitoring technique for large-scale structures but is vulnerable to changes in environmental and operational conditions(EOC),which are inevitable in the normal inspection of ci... Ultrasonic guided wave is an attractive monitoring technique for large-scale structures but is vulnerable to changes in environmental and operational conditions(EOC),which are inevitable in the normal inspection of civil and mechanical structures.This paper thus presents a robust guided wave-based method for damage detection and localization under complex environmental conditions by singular value decomposition-based feature extraction and one-dimensional convolutional neural network(1D-CNN).After singular value decomposition-based feature extraction processing,a temporal robust damage index(TRDI)is extracted,and the effect of EOCs is well removed.Hence,even for the signals with a very large temperature-varying range and low signal-to-noise ratios(SNRs),the final damage detection and localization accuracy retain perfect 100%.Verifications are conducted on two different experimental datasets.The first dataset consists of guided wave signals collected from a thin aluminum plate with artificial noises,and the second is a publicly available experimental dataset of guided wave signals acquired on a composite plate with a temperature ranging from 20℃to 60℃.It is demonstrated that the proposed method can detect and localize the damage accurately and rapidly,showing great potential for application in complex and unknown EOC. 展开更多
关键词 Ultrasonic guided waves Singular value decomposition Damage detection and localization Environmental and operational conditions One-dimensional convolutional neural network
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新冠病毒变异株RBD二聚体mRNA疫苗广谱免疫原性研究 被引量:1
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作者 赵旭 吴鑫凯 +16 位作者 杜沛 陈茜 马雪慧 胡世雄 吴春丽 杨惠婷 马任义 李爽 孔天翔 李睿琦 冯英浩 王晓云 荣笑雨 郑安琪 陆剑 高福 王奇慧 《科学通报》 EI CAS CSCD 北大核心 2024年第33期4905-4916,共12页
严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)是导致新冠病毒病(coronavirus disease 2019,COVID-19)的病原体.2019年末至今,SARS-CoV-2在全球范围流行,氨基酸突变累积产生多种具有较高... 严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)是导致新冠病毒病(coronavirus disease 2019,COVID-19)的病原体.2019年末至今,SARS-CoV-2在全球范围流行,氨基酸突变累积产生多种具有较高传播力或较强免疫逃逸能力的变异株.目前广泛使用的COVID-19疫苗大多基于SARS-CoV-2原型株(prototype,PT)进行免疫原设计.这些疫苗针对早期变异株具有较好的保护效果,然而面对后续出现的Omicron系列变异株,以原型株为免疫原的疫苗保护效力显著下降,特别是在面对免疫逃逸极强的BF.7、BQ.1.1、CH.1.1、XBB及XBB.1.5等变异株时,难以激发较高水平特异性中和抗体.因此,亟须研发针对多种SARS-CoV-2变异株产生高效中和抗体的新一代广谱疫苗.本研究基于SARS-CoV-2受体结合域(receptor-binding domain,RBD)重复串联二聚体的构型,设计了6种包含Delta RBD以及BA.1、BA.2和BA.5等Omicron亚型RBD的串联二聚体mRNA疫苗,并对其表达情况及免疫原性进行了系统性评价.同时,还验证了在两针灭活疫苗基础上以mRNA疫苗作为加强针的序贯免疫效果.假病毒中和数据显示,Delta-BA.2(DO2)与Delta-BA.5(DO5)RBD二聚体疫苗表现出较优的免疫原性,且两者诱导的中和抗体分别针对BA.2之前和BA.5之后出现的变异株有较好的中和活性,表现出一定的互补趋势.因此,我们设计了DO2和DO5混合免疫的策略,实现了对二者优势的互补,进一步扩宽了疫苗中和抗体谱.在三剂免疫的结果中,DO5及DO2+DO5免疫可产生针对CH.1.1、XBB及XBB.1.5的高水平中和抗体.这些结果有助于我们理解不同分支变异株中和抗体谱的变化规律,并为广谱COVID-19疫苗设计提供了参考. 展开更多
关键词 SARS-CoV-2 Omicron变异株 mRNA疫苗 广谱疫苗
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基于RBD抗原性的五种SARS-CoV-2血清型分类 被引量:3
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作者 胡世雄 吴春丽 +9 位作者 吴鑫凯 马雪慧 舒畅 陈茜 郑安琪 杨惠婷 陆剑 杜沛 高福 王奇慧 《Science Bulletin》 SCIE EI CAS CSCD 2023年第23期3003-3012,M0005,共11页
新冠病毒(SARS-CoV-2)的不断进化带来了大量的变异株,特别是Omicron变异株及其众多的亚型.这些变异株表现出越来越强的免疫逃逸能力,使现有疫苗和治疗抗体的效力不断下降.目前,众多变异株已表现出血清交叉中和作用减弱的现象,表明新冠... 新冠病毒(SARS-CoV-2)的不断进化带来了大量的变异株,特别是Omicron变异株及其众多的亚型.这些变异株表现出越来越强的免疫逃逸能力,使现有疫苗和治疗抗体的效力不断下降.目前,众多变异株已表现出血清交叉中和作用减弱的现象,表明新冠病毒可能已进化出多种血清型.因此,我们选取新冠病毒的主要抗原,即刺突(S)蛋白的受体结合域(RBD)对其进行血清分型.我们首先选择了23个具有代表性的新冠病毒毒株,涵盖了前Omicron变异株和Omicron变异株的多种亚型.通过对RBD抗原性的系统评估,我们将23种变异株分为5种血清型,每种血清型都包含了数种基因型不同的变异株.具体而言,Ⅰ型涵盖了所有前Omicron变异株(含两种亚型),而其余四种血清型均包含处于不同进化阶段的Omicron亚型.本文中的血清分型可以为新型变异株的快速评估奠定基础,并指导未来针对新冠病毒的广谱疫苗和中和抗体的开发. 展开更多
关键词 SARS-CoV-2 Serotype classification mRNA vaccine Spike(S) Receptor-binding domain(RBD)
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