Displacement field reconstruction in landslide physical modeling by using a terrain laser scanner e Part 2:Application and large strain/displacement and water effect analysis

Deformation analysis is fundamental in geotechnical modeling.Nevertheless,there is still a lack of an effective method to obtain the deformation field under various experimental conditions.In this study,we introduce a processebased physical modeling of a pileereinforced reservoir landslide and present an improved deformation analysis involving large strains and water effects.We collect multieperiod point clouds using a terrain laser scanner and reconstruct its deformation field through a point cloud processing workflow.The results show that this method can accurately describe the landslide surface deformation at any time and area by both scalar and vector fields.The deformation fields in different profiles of the physical model and different stages of the evolutionary process provide adequate and detailed landslide information.We analyze the large strain upstream of the pile caused by the pile installation and the consequent violent deformation during the evolutionary process.Furthermore,our method effectively overcomes the challenges of identifying targets commonly encountered in geotechnical modeling where water effects are considered and targets are polluted,which facilitates the deformation analysis at the wading area in a reservoir landslide.Eventually,combining subsurface deformation as well as numerical modeling,we comprehensively analyze the kinematics and failure mechanisms of this complicated object involving landslides and pile foundations as well as water effects.This method is of great significance for any geotechnical modeling concerning large-strain analysis and water effects.

Displacement field reconstruction in landslide physical modeling by using a terrain laser scanner e Part 1:Methodology,error analysis and validation

Laser scanning technology has been widely used in landslide aspects.However,the existing deformation analysis based on terrain laser scanners can only provide limited information,which is insufficient for understanding landslide kinematics and failure mechanisms.To overcome this limitation,this paper proposes an automated method for processing point clouds collected in landslide physical modeling.This method allows the acquisition of quantitative three-dimensional(3D)deformation field information.The results show the organized and spatially related point cloud segmentation in terms of spherical targets.The segmented point clouds can be fitted to determine the locations of all preset targets and their corresponding location changes.The proposed method has been validated based on theoretical analysis and numerical and physical tests,which indicates that it can batch-process massive data sets with high computational efficiency and good noise resistance.Compared to existing methods,this method shows a significant potential for understanding landslide kinematics and failure mechanisms and advancing the application of 3D laser scanning in geotechnical modeling.

Research on Reservoir Landslide Thrust Based on Improved Morgenstern-Price Method

The curve of landslide thrust plays a key role in landslide design.The commonly used transfer coefficient method(TCM)and Morgenstern-Price method(MPM)are analyzed.TCM does not take into account the moment balance between slices.Although MPM considers the moment balance,the calculation is complex,and it does not consider that the force between slices may be less than zero at the back edge of the landslide.The rationality and feasibility of the improved MPM are verified by calculating the landslide stability coefficient and landslide thrust at different reservoir water levels.This paper studies the law of landslide thrust when the reservoir water level changes,and discusses the determination of design thrust,to provide a certain theoretical basis for the design of reservoir landslides.

Study on Shear Strength Characteristics of Basalt-Concrete Bonding Interface Based on in-situ Direct Shear Test

In rock engineering,the shear strength of the basalt-concrete bonding interface is a key factor affecting the shear performance of hydroelectric dam foundations,embedded rock piles and rock bolts.In this study,30 sets of in-situ direct shear tests were conducted on the basalt-concrete bond interface in the Baihetan dam area to investigate the shear strength characteristics of the basalt-concrete bonding interface.The bonding interface contains two states,i.e.,the bonding interface is not sheared,termed as se(symbolic meaning see Table 1);the bonding interface is sheared with rupture surface,termed as si.The effects of lithology,Joints structure,rock type grade and concrete compressive strength on the shear strength of the concrete-basalt contact surface were investigated.The test results show that the shear strength of the bonding interface(s_(e)&s_(i))of columnar jointed basalt with concrete is greater than that of the bonding interface(s_(e)&s_(i))of non-columnar jointed one with the same rock type grade.When the rock type grade isⅢ_(2),fcol is 1.22 times higher than fncol and ccol is 1.13 times greater than cncol.The shear strength parameters of the basalt-concrete bonding interface differ significantly for different lithologies.The cohesion of the bonding interface(s_(i))of cryptocrystalline basalt with concrete is 2.05 times higher than that of the bonding interface(s_(i))of breccia lava with concrete under the same rock type grade condition.Rock type grade has a large influence on the shear strength of the non-columnar jointed basalt-concrete bonding interface(s_(e)&s_(i)).cnol increases by 33%when the grade of rock type rises fromⅢ_(1)toⅡ_(1).the rock type grade has a greater effect on bonding interface(s_(i))cohesion than the coefficient of friction.When the rock type grade is reduced fromⅢ_(2)toⅢ_(1),f_(ncol)′increases by 2%and c_(ncol)′improves by 44%.The shear strength of the non-columnar jointed basalt-concrete bonding interface(s_(e)&s_(i))increases with the increase of the compressive strength of concrete.When concrete compressive strength rises from 22.2 to 27.6 MPa,the cohesion increases by 94%.

Slope Stability Analysis Based on Group Decision Theory and Fuzzy Comprehensive Evaluation

A slope stability evaluation method is proposed combining group decision theory,the analytic hierarchy process and fuzzy comprehensive evaluation.The index weight assignment of each evaluation element is determined by group decision theory and the analytic hierarchy process,and the membership degree of each indicator is determined based on fuzzy set theory.According to the weights and memberships,the membership degrees of the criterion layer are obtained by fuzzy operations to evaluate the slope stability.The results show that(1)the evaluation method comprehensively combines the effects of multiple factors on the slope stability,and the evaluation results are accurate;(2)the evaluation method can fully leverage the experience of the expert group and effectively avoid evaluation errors caused by the subjective bias of a single expert;(3)based on a group decision theory entropy model,this evaluation method can quantitatively evaluate the reliability of expert decisions and effectively improve the efficiency of expert group discussion;and(4)the evaluation method can transform the originally fuzzy and subjective slope stability evaluation into a quantitative evaluation.

A Constitutive Model of Granite Shear Creep under Moisture

Previous constitutive models of granite shear creep have two limitations：（1） although moisture greatly affects granite shear creep behavior, currently there are no constitutive models that include this factor;（2） there are also no models that include an acceleration stage. This paper presents an improved Burgers constitutive model with the addition of a damage parameter to characterize the moisture effect and uses a nonlinear relation equation between stress and strain for inclusion as the acceleration stage. The damage parameter is determined from granite creep experiment under four different moisture contents（0%, 0.22%, 0.49%, and 0.79%）. The nonlinear relation equation is obtained by fitting a dataset of stain versus time under five different loading stages. To verify the presented model, a creep experiment was conducted on other granite samples and the results show that the model agrees well with the experimental observation data.

Study on Shear Strength Characteristics of Columnar Jointed Basalt Based on in-situ Direct Shear Test at Baihetan Hydropower Station

Columnar jointed basalt(CJB) widely distributes in the dam site of the Baihetan Hydropower Station.The columnar joint structure and fracture development of CJB have significant influence on the mechanical properties of rock mass,and the mechanical properties of CJB are of great significance to the Baihetan Hydropower Project.Therefore,in-situ direct shear tests were carried out on ten test adit at different locations in the dam site area to study the shear behavior of CJB.In this study,21 sets of in-situ direct shear tests were conducted for rock types of type Ⅱ_(2),type Ⅲ_(1)and type Ⅲ_(2),with horizontal and vertical shear planes and two different specimen sizes of CJB.Shear strength parameters of CJB were obtained by linear fitting of in-situ direct shear test results based on the Mohr-Coulomb strength criterion.The results indicate that the shear strength parameters of CJB with horizontal shear plane increase as the increase of rock type grade.The shear strength parameters of CJB show obvious anisotropy and the friction coefficient of the horizontal shear plane is greater than the vertical shear plane.The friction coefficient in the horizontal direction of the shear plane is 1.27 times that in the vertical direction of the shear plane.With the increase of rock type grade,the difference of friction coefficient becomes larger.However,the cohesion changes little whether the shear plane is horizontal or vertical.In addition,the size effect of CJB in this area is significant.The shear strength parameters of large size(100 cm × 100 cm) specimens are lower than those of regular size(50 cm × 50 cm) specimens.The reduction of cohesion is greater than that of the friction coefficient.For rock type Ⅲ_(2),the cohesion of large-size specimens is 0.637 of the regular-size specimens.The reduction percentage of the friction coefficient for type Ⅲ_(2)is 1.66 times that of type Ⅲ_(1).The reduction percentage of the cohesion for type Ⅲ_(2)is 1.27 times that of type Ⅲ_(1).The size effect decreases with the increase of rock type grade.The research results of this study can provide an important basis for the selection of rock mechanics parameters in the dam site area of Baihetan Hydropower Station and the stability analysis of the dam foundation and rocky slopes.

E3 ligase MG53 suppresses tumor growth by degrading cyclin D1

Due to the essential role of cyclin D1 in regulating transition from G1 to S phase in cell cycle,aberrant cyclin D1 expression is a major oncogenic event in many types of cancers.In particular,the dysregulation of ubiquitination-dependent degradation of cyclin D1 contributes to not only the pathogenesis of malignancies but also the refractory to cancer treatment regiments with CDK4/6 inhibitors.Here we show that in colorectal and gastric cancer patients,MG53 is downregulated in more than 80%of tumors compared to the normal gastrointestinal tissues from the same patient,and the reduced MG53 expression is correlated with increased cyclin D1 abundance and inferior survival.Mechanistically,MG53 catalyzes the K48-linked ubiquitination and subsequent degradation of cyclin D1.Thus,increased expression of MG53 leads to cell cycle arrest at G1,and thereby markedly suppresses cancer cell proliferation in vitro as well as tumor growth in mice with xenograft tumors or AOM/DSS induced-colorectal cancer.Consistently,MG53 deficiency results in accumulation of cyclin D1 protein and accelerates cancer cell growth both in culture and in animal models.These findings define MG53 as a tumor suppressor via facilitating cyclin D1 degradation,highlighting the therapeutic potential of targeting MG53 in treating cancers with dysregulated cyclin D1 turnover.

Bi-FoRe:an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators

Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function.However,contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been reported.Here we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NHEJ-mediated unidirectional targeted insertion in zebrafish facilitated by the CRISPR/Cas system.We demonstrated the feasibility of this strategy at sox10 and isl1 loci,and successfully achieved Cre-induced conditional knockout of target gene function and simultaneous switch of the fluorescent reporter,allowing generation of genetic mosaics for lineage tracing.We then improved the donor design enabling efficient one-step bidirectional knockin to generate paired positive and negative conditional alleles,both tagged with two different fluorescent reporters.By introducing Cre recombinase,these alleles could be used to achieve both conditional knockout and conditional gene restoration in parallel;furthermore,differential fluorescent labeling of the positive and negative alleles enables simple,early and efficient realtime discrimination of individual live embryos bearing different genotypes prior to the emergence of morphologically visible phenotypes.We named our improved donor as Bi-FoRe and demonstrated its feasibility at the sox10 locus.Furthermore,we eliminated the undesirable bacterial backbone in the donor using minicircle DNA technology.Our system could easily be expanded for other applications or to other organisms,and coupling fluorescent labeling of gene expression and conditional manipulation of gene function will provide unique opportunities to fully reveal the power of emerging single-cell sequencing technologies.

sRAGE alleviates SARS-CoV-2-induced pneumonia in hamster

Dear Editor Since last year,the most demanding task of the global pharmaceutical community has been focused on the development of strategies to treat coronavirus disease 2019(COVID-19).To date,several vaccines have been developed and already demonstrated their efficacy in reducing the incidence of COVID-19.1 However,the development of drugs treating COVID-19 is lagging far behind,and all the current treatment regimens have their limitations.