Effect of sodium starch octenyl succinate-based Pickering emulsion on the physicochemical properties of hairtail myofibrillar protein gel subjected to multiple freeze-thaw cycles

A Pickering emulsion based on sodium starch octenyl succinate(SSOS)was prepared and its effects on the physicochemical properties of hairtail myofibrillar protein gels(MPGs)subjected to multiple freeze-thaw(F-T)cycles were investigated.The whiteness,water-holding capacity,storage modulus(G')and texture properties of the MPGs were significantly improved by adding 1%-2%Pickering emulsion(P<0.05).Meanwhile,Raman spectral analysis demonstrated that Pickering emulsion promoted the transformation of secondary structure,enhanced hydrogen bonds and hydrophobic interactions,and promoted the transition of disulfide bond conformation from g-g-g to g-g-t and t-g-t.At an emulsion concentration of 2%,theα-helix content decreased by 10.37%,while theβ-sheet content increased by 7.94%,compared to the control.After F-T cycles,the structure of the MPGs was destroyed,with an increase in hardness and a decrease in whiteness and water-holding capacity,however,the quality degradation of MPGs was reduced with 1%-2%Pickering emulsion.These findings demonstrated that SSOS-Pickering emulsions,as potential fat substitutes,can enhance the gel properties and the F-T stability of MPGs.

Artificial intelligence and evidence-based research will promote the development of traditional medicine

Traditional medicine(TM)has progressively achieved international recognition.The recent first-ever World Health Organization(WHO)Traditional Medicine Global Summit 2023 has undoubtedly highlighted the significance of TM^([1]).This conference was held in India during August 17–18,2023^([2]),aiming to mobilize political commitment and evidence-based action supporting TM and integrating it to advance the health and well-being of the people and planet.

The investigation of DARC etch back in DRAM capacitor oxide mask opening

Opening the silicon oxide mask of a capacitor in dynamic random access memory is a critical process on a capacitive coupled plasma(CCP)etch tool.Three steps,dielectric anti-reflective coating(DARC)etch back,silicon oxide etch and strip,are contained.To acquire good performance,such as low leakage current and high capacitance,for further fabricating capacitors,we should firstly optimize DARC etch back.We developed some experiments,focusing on etch time and chemistry,to evalu-ate the profile of a silicon oxide mask,DARC remain and critical dimension.The result shows that etch back time should be con-trolled in the range from 50 to 60 s,based on the current equipment and condition.It will make B/T ratio higher than 70%mean-while resolve the DARC remain issue.We also found that CH_(2)F_(2) flow should be~15 sccm to avoid reversed CD trend and keep in-line CD.

Research and Implementation of a License Plate Recognition Algorithm Based on Hierarchical Classification

This paper proposed an improved method for license plate recognition based on hierarchical classification. First, the method of feature extraction and dimension reduction is presented by finding the optimal wavelet packet basis in the process of wavelet packet decomposition and K-L transform. Then the recognition algorithm is introduced based on feature extraction and hierarchical classification. Finally, the principles and procedures of using support vector machines, Harris corner detection algorithm and digital character classification are explained in detail. Simulation results indicate that the presented recognition algorithm performs well with higher speed and efficiency in recognition.

数字化下颌运动分析系统辅助前牙区多单位功能性精准修复1例

目的:报道1例借助数字化下颌运动分析系统进行下颌运动分析,实现前牙区多单位功能性精准修复的病例。诊治经过:借助数字化下颌运动分析系统对患者修复前的下颌运动进行数据分析,评估旧修复体存在的问题并设计制作诊断蜡型。临时修复后再次测量下颌运动数据以评估其与设计方案的一致性。3个月后,换用最终修复体,并再次使用数字化下颌运动分析系统进行下颌运动数据分析。结果:最终修复后,冠边缘适合性、邻面接触点、咬合接触及下颌功能运动良好。结论:借助数字化下颌运动分析系统获取下颌运动过程中的相关精确数据,根据数据实现术前设计、术中调整、术后验证,可以在前牙区多单位修复中恢复良好的前牙引导功能。

Effective peroxydisulfate activation by CQDs-MnFe_(2)O_(4)@ZIF-8 catalyst for complementary degradation of bisphenol A by free radicals and non-radical pathways

The present study reported fabrication of novel carbon quantum dots-MnFe_(2)O_(4)@ZIF-8(CQDs-MFO@ZIF-8)by using co-precipitation hydrothermal method for activation of peroxydisulfate(PDS)to degrade bisphenol A(BPA),one of important emerging organic pollutants in water environment.CQDs-MFO@ZIF-8 served as a highly efficient thermal activated PDS catalyst with high catalytic degradation efficiency,reusability and stability.The catalyst achieved almost completely removal of 20.0 mg/L BPA within 5.0 min,and the degradation efficiency remained higher than 83%after 5 consecutive cycles.Free radicals(^(·)OH,SO_(4)^(·-)and^(·)O_(2)^(-))and non-free radicals((1)O_(2))were generated in the thermal PDS-activation system,in which singlet oxygen((1)O_(2))played a dominant role in the degradation of BPA.The potential toxicity of BPA degradation intermediates was analyzed upon the culture of E.coli and Chlorella sorokiniana by using Ecological Structure-Activity Relationship Model(ECOSAR)program.The catalytic performances of BPA degradation by CQDs-MFO@ZIF-8 were evaluated for treatment of different practical water samples to further verify the feasibility of practical applications.This study provides proof-in-concept demonstration of new nanomaterials for enhanced catalytic water decontamination.

Kidney injury molecule-1 is a potential receptor for SARS-CoV-2

COVID-19 patients present high incidence of kidney abnormalities,which are associated with poor prognosis and mortality.The identification of SARS-CoV-2 in the kidney of COVID-19 patients suggests renal tropism of SARS-CoV-2.However,whether there is a specific target of SARS-CoV-2 in the kidney remains unclear.Herein,by using in silico simulation,coimmunoprecipitation,fluorescence resonance energy transfer,fluorescein isothiocyanate labeling,and rational design of antagonist peptides,we demonstrate that kidney injury molecule-1(KIM1),a molecule dramatically upregulated upon kidney injury,binds with the receptor-binding domain(RBD)of SARS-CoV-2 and facilitates its attachment to cell membrane,with the immunoglobulin variable Ig-like(Ig V)domain of KIM1 playing a key role in this recognition.The interaction between SARS-CoV-2 RBD and KIM1 is potently blockaded by a rationally designed KIM1-derived polypeptide AP2.In addition,our results also suggest interactions between KIM1 Ig V domain and the RBDs of SARS-CoV and MERS-CoV,pathogens of two severe infectious respiratory diseases.Together,these findings suggest KIM1 as a novel receptor for SARS-CoV-2 and other coronaviruses.We propose that KIM1 may thus mediate and exacerbate the renal infection of SARS-CoV-2 in a‘vicious cycle’,and KIM1 could be further explored as a therapeutic target.

Implantable Nanofiber Membranes with Synergistic Photothermal and Autophagy Inhibition Effects for Enhanced Tumor Therapy Efficacy

Photothermal therapy(PTT)has been proposed as an advanced patient-centered strategy for tumor treatment.Nevertheless,the uncertain safety of conventional photothermal conversion agents and the presence of intracellular self-protective autophagy mechanisms pose obstacles to the clinical application and efficacy of PTT.As we are deeply aware of the seriousness of these problems,we herein proposed an efficacy-enhancing strategy based on an implantable membrane platform(PPG@PB-HCQ)constructed from poly(lactic acid)(PLA),poly(ɛ-caprolactone)(PCL)and gelatin(Gel)electrospun nanofibers(PPG)and loaded with the biodegradable high-efficiency photothermal conversion agent Prussian blue(PB)and the autophagy inhibitor hydroxychloroquine sulfate(HCQ).Cellular experiments confirmed that the PPG@PB-HCQ nanofiber membrane exhibited a significantly stronger tumor cell-killing effect compared with the PTT alone.This enhancement features by of blocking the fusion of autophagosomes with lysosomes.The intracellular overexpression of the proteins microtubule-associated protein 1 light chain 3(LC3)-II and p62 and the low expression of the proteins LC3-I and Rab7(members of the RAS oncogene family)further demonstrated autophagic flux blockade.Importantly,the potent antitumor effect of the PPG@PB-HCQ therapeutic platform in B16 tumor-bearing model mice verified the efficacy-enhancing strategy of synergistic PTT and protective autophagy blockade.The present study provides a promising strategy for solving the difficulties of tumor treatment,as well as a new perspective for designing novel treatment platforms.