This paper has analyzed allelopathic effects ofNerium indicum on Oncomelania hupensis through triterpene sapogenins, a potential molluscicide. The snails were treated under six various concentrations (0, 20, 40, 60, ...This paper has analyzed allelopathic effects ofNerium indicum on Oncomelania hupensis through triterpene sapogenins, a potential molluscicide. The snails were treated under six various concentrations (0, 20, 40, 60, 80, 100 mg/L) of triterpene sapogenins and five periods (1, 2, 3, 4, 5 days). The mortality of snails was positively correlated with the concentration of triterpene sapogenins and exposure time. The results ofprobit analysis showed that the LD50 (Lethal Dose, 50%) oftriterpenoid saponins from N. indicum by immersion for 2, 3, 4, 5 days were 78.31, 30.26, 20.50, 14.19 mg/L, respectively. And the corresponding 95% confidence intervals were 63.60-108.19, 9.49-44.42, 2.86-30.90, 0.23-22.79 mg/L, respectively. The observations of both scanning electron microscope and transmission electron microscope proved that 40 mg/L triterpene sapogenins could cause apparent damage to the structure of soft tissue, liver and intestine of O. hupensis. The esterase (EST) isozyme electrophoresis in liver of O. hupensis treated by 40 mg/L of the concentrations lixivium of the triterpene sapogenins from N. indicum was analyzed for 24, 48, 72, 96, 120 h, respectively. The activity of enzyme was higher than control water group after been treated up to 24-48 h, and then lowered and disappeared after 72 h. It was implicated that the extracted triterpene sapogenin from N. indicum were promising for controlling the snail, which were also providing the foundation for constructing plant community of oleander to control O. hupensis.展开更多
The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin produ...The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin production,but the underlying regulatory mechanism remains elusive.In this study,we demonstrate that ELONGATED HYPOCOTYL5(HY5),a central transcription factor in the light signaling pathway,cannot promote artemisinin biosynthesis on its own,as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their transcription.Transcriptome analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with AaHY5.AaBBX21 showed a trichome-specific expression pattern.Additionally,the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1,AaMYB108,and AaORA,encoding positive regulators of artemisinin biosynthesis.Moreover,AaHY5 and AaBBX21 physically interacted with the A.annua E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1).In the dark,AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex,explaining the enhanced production of artemisinin upon light exposure.Our study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.展开更多
This research investigates retrofitting strategies for multifunctional spaces within educational buildings,employing agent-based and performance-based modeling to support decision-making.An experimental matrix was dev...This research investigates retrofitting strategies for multifunctional spaces within educational buildings,employing agent-based and performance-based modeling to support decision-making.An experimental matrix was developed,reflecting three usage scenarios(reading,exhibition,lecture)across four retrofitting schemes.An agent-based model was developed to delineate intricate human behaviors in space and examined the self-organizing behaviors of 30 agents for each scheme in every scenario,evaluating six metrics on spatial efficiency and visual experience.Calibrated models,derived from real data and processed through DesignBuilder software,evaluated three metrics:energy use,thermal comfort,and visual comfort.The research then incorporated metrics from the agent-based model and performance simulation to develop a method for discussing the decision-making process in retrofit strategies.The findings indicate that the optimal retrofitting solution for multifunctional spaces is heavily influenced by the distribution of usage scenarios.Given the substantial influence of space metrics on selecting the optimal retrofit scheme,the proposed framework effectively facilitates decision-making for building retrofits by providing a holistic evaluation of both spatial and energy criteria.展开更多
Organic pollutants are widespread environmental pollutants with high toxicity,persistence,and bioaccumulation.Our aim was to investigate the distribution of aromatic amines,phenols,chlorobenzenes,and naphthalenes in t...Organic pollutants are widespread environmental pollutants with high toxicity,persistence,and bioaccumulation.Our aim was to investigate the distribution of aromatic amines,phenols,chlorobenzenes,and naphthalenes in the surface sediment of the Dianchi Lake,China.Nineteen surface sediment samples were collected from the Dianchi Lake,and 40 types of organic pollutants were analyzed via gas chromatography-mass spectrometry.The total organic pollutant concentrations in the surface sediment of the Dianchi Lake varied from 27.4 to 1.62×10^3 ng/g.The concentrations of phenols were much higher than those in other water bodies but still within a controllable range,whereas the concentrations of the other organic pollutant classes were similar or even lower.The detection ratio of 3-or 4-methylphenol was the highest(100.00%)among the pollutants.The average total organic pollutant concentrations decreased in the following order:Caohai(540 ng/g)>the middle of Waihai(488 ng/g)>the edge of Waihai(351 ng/g)>Haigeng Dam(90.4 ng/g).Pearson analysis showed a strong correlation among 1-methylnaphthalene,2-methylnaphthalene,1,3-dinitronaphthalene,and 1,4-dinitronaphthalene(P<0.01).Caohai,the north lakeshore of Waihai and the south of Waihai showed higher risk because of high concentration;meanwhile,1,4-dichlorobenzene,3-or 4-methylphenol and 1,2,4-trichlorobenzene were mor e likely to cause risks.展开更多
Direct methanol fuel cells have the advantages of simple system,convenient operation,high conversion rate and low carbon emission,which are considered as the environmental and friendly energy conversion devices.How-ev...Direct methanol fuel cells have the advantages of simple system,convenient operation,high conversion rate and low carbon emission,which are considered as the environmental and friendly energy conversion devices.How-ever,the low activity,CO-tolerance and high cost of anode catalysts restrict the large-scale commercial appli-cations.Therefore,it is of great practical significance to design and construct the anodic catalysts with high activity,stability and low cost for methanol oxidation reaction.In this work,the PtM/Nb_(2)O_(5)-C(M=Co,Sn,Ni)catalysts are synthesized by the ethylene glycol solvothermal method using transition metal oxide Nb_(2)O_(5)as the support.The catalytic performance of different catalysts is further evaluated for alkaline MOR.The results show that the introduction of Ni(existing in Ni^(2+)and Ni^(3+))has the most obvious improvement for alkaline MOR performance.By adjusting the doped ratio of Pt:Ni,it is shown that PtNi/Nb_(2)O_(5)-C has the highest mass activity(3877.9 mA-mg_(pt)^(-1)),12 times that of the commercial Pt/C catalyst.CV,LSV,Tafel and EIS analyses show that PtNi/Nb_(2)O_(5)-C has the lowest onset potential and charge transfer resistance,and the fastest electrocatalytic oxidation rate of methanol.CA tests show that the electrochemical stability is also significantly improved with the introduction of Nb_(2)O_(5)and Ni.Combined with the structural characterization and electrochemical tests,it is found that the evident electronic effect among Pt and Ni,Nb_(2)O_(5)and the hydroxyl brought from Ni species are mainly ascribed for enhancing the activity,CO resistance and stability of PtNi/Nb_(2)O_(5)-C.展开更多
基金Acknowledgments The authors would like to thank the National Natural Science Foundation of China (grant No. 30471506, 30570322, 39670654, 30671818) and the Science and Technology Foundation of Forest (No. 2006BAD03AI 5) for providing funds to the research.
文摘This paper has analyzed allelopathic effects ofNerium indicum on Oncomelania hupensis through triterpene sapogenins, a potential molluscicide. The snails were treated under six various concentrations (0, 20, 40, 60, 80, 100 mg/L) of triterpene sapogenins and five periods (1, 2, 3, 4, 5 days). The mortality of snails was positively correlated with the concentration of triterpene sapogenins and exposure time. The results ofprobit analysis showed that the LD50 (Lethal Dose, 50%) oftriterpenoid saponins from N. indicum by immersion for 2, 3, 4, 5 days were 78.31, 30.26, 20.50, 14.19 mg/L, respectively. And the corresponding 95% confidence intervals were 63.60-108.19, 9.49-44.42, 2.86-30.90, 0.23-22.79 mg/L, respectively. The observations of both scanning electron microscope and transmission electron microscope proved that 40 mg/L triterpene sapogenins could cause apparent damage to the structure of soft tissue, liver and intestine of O. hupensis. The esterase (EST) isozyme electrophoresis in liver of O. hupensis treated by 40 mg/L of the concentrations lixivium of the triterpene sapogenins from N. indicum was analyzed for 24, 48, 72, 96, 120 h, respectively. The activity of enzyme was higher than control water group after been treated up to 24-48 h, and then lowered and disappeared after 72 h. It was implicated that the extracted triterpene sapogenin from N. indicum were promising for controlling the snail, which were also providing the foundation for constructing plant community of oleander to control O. hupensis.
基金supported by the National Natural Science Foundation of China (32070329, 31770327)Shanghai Natural Science Foundation (16ZR1418000)the National Key R&D Program of China (2018Y FA0900600)。
文摘The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin production,but the underlying regulatory mechanism remains elusive.In this study,we demonstrate that ELONGATED HYPOCOTYL5(HY5),a central transcription factor in the light signaling pathway,cannot promote artemisinin biosynthesis on its own,as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their transcription.Transcriptome analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with AaHY5.AaBBX21 showed a trichome-specific expression pattern.Additionally,the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1,AaMYB108,and AaORA,encoding positive regulators of artemisinin biosynthesis.Moreover,AaHY5 and AaBBX21 physically interacted with the A.annua E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1).In the dark,AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex,explaining the enhanced production of artemisinin upon light exposure.Our study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.
基金sponsored by the National Science and Foundation of China(No.52208011)the Natural Science and Foundation of China(NSFC No.52208010)the China Postdoctoral Science Foundation(No.2022M720716).
文摘This research investigates retrofitting strategies for multifunctional spaces within educational buildings,employing agent-based and performance-based modeling to support decision-making.An experimental matrix was developed,reflecting three usage scenarios(reading,exhibition,lecture)across four retrofitting schemes.An agent-based model was developed to delineate intricate human behaviors in space and examined the self-organizing behaviors of 30 agents for each scheme in every scenario,evaluating six metrics on spatial efficiency and visual experience.Calibrated models,derived from real data and processed through DesignBuilder software,evaluated three metrics:energy use,thermal comfort,and visual comfort.The research then incorporated metrics from the agent-based model and performance simulation to develop a method for discussing the decision-making process in retrofit strategies.The findings indicate that the optimal retrofitting solution for multifunctional spaces is heavily influenced by the distribution of usage scenarios.Given the substantial influence of space metrics on selecting the optimal retrofit scheme,the proposed framework effectively facilitates decision-making for building retrofits by providing a holistic evaluation of both spatial and energy criteria.
基金Th e authors appreciate the financial support from the National Key R&D Program of China(2018YFC1903000)the Fundamental Research Funds for Central Universities(2019QNYL23)the Key Laboratory of Eco-geochemistry,Ministry of Natural Resources,China(CSJ2017012).
文摘Organic pollutants are widespread environmental pollutants with high toxicity,persistence,and bioaccumulation.Our aim was to investigate the distribution of aromatic amines,phenols,chlorobenzenes,and naphthalenes in the surface sediment of the Dianchi Lake,China.Nineteen surface sediment samples were collected from the Dianchi Lake,and 40 types of organic pollutants were analyzed via gas chromatography-mass spectrometry.The total organic pollutant concentrations in the surface sediment of the Dianchi Lake varied from 27.4 to 1.62×10^3 ng/g.The concentrations of phenols were much higher than those in other water bodies but still within a controllable range,whereas the concentrations of the other organic pollutant classes were similar or even lower.The detection ratio of 3-or 4-methylphenol was the highest(100.00%)among the pollutants.The average total organic pollutant concentrations decreased in the following order:Caohai(540 ng/g)>the middle of Waihai(488 ng/g)>the edge of Waihai(351 ng/g)>Haigeng Dam(90.4 ng/g).Pearson analysis showed a strong correlation among 1-methylnaphthalene,2-methylnaphthalene,1,3-dinitronaphthalene,and 1,4-dinitronaphthalene(P<0.01).Caohai,the north lakeshore of Waihai and the south of Waihai showed higher risk because of high concentration;meanwhile,1,4-dichlorobenzene,3-or 4-methylphenol and 1,2,4-trichlorobenzene were mor e likely to cause risks.
基金National Natural Science Foundation of China(22075225,21706203,22038011 and 22005236)Natural Science Basic Research Plan in Shaanxi Province of China(2022JZ-07)+1 种基金State Key Laboratory of Clean and Efficient Coal Utilization,Taiyuan University of Technology(MJNYSKL202308)Key Research and Development Program in Shaanxi Province(2022QCY-LL-16).
文摘Direct methanol fuel cells have the advantages of simple system,convenient operation,high conversion rate and low carbon emission,which are considered as the environmental and friendly energy conversion devices.How-ever,the low activity,CO-tolerance and high cost of anode catalysts restrict the large-scale commercial appli-cations.Therefore,it is of great practical significance to design and construct the anodic catalysts with high activity,stability and low cost for methanol oxidation reaction.In this work,the PtM/Nb_(2)O_(5)-C(M=Co,Sn,Ni)catalysts are synthesized by the ethylene glycol solvothermal method using transition metal oxide Nb_(2)O_(5)as the support.The catalytic performance of different catalysts is further evaluated for alkaline MOR.The results show that the introduction of Ni(existing in Ni^(2+)and Ni^(3+))has the most obvious improvement for alkaline MOR performance.By adjusting the doped ratio of Pt:Ni,it is shown that PtNi/Nb_(2)O_(5)-C has the highest mass activity(3877.9 mA-mg_(pt)^(-1)),12 times that of the commercial Pt/C catalyst.CV,LSV,Tafel and EIS analyses show that PtNi/Nb_(2)O_(5)-C has the lowest onset potential and charge transfer resistance,and the fastest electrocatalytic oxidation rate of methanol.CA tests show that the electrochemical stability is also significantly improved with the introduction of Nb_(2)O_(5)and Ni.Combined with the structural characterization and electrochemical tests,it is found that the evident electronic effect among Pt and Ni,Nb_(2)O_(5)and the hydroxyl brought from Ni species are mainly ascribed for enhancing the activity,CO resistance and stability of PtNi/Nb_(2)O_(5)-C.