Test and Analysis of the Sound Insulation Performance of Four Types of Timber Structure Floors under Jumping Excitation

To improve the impact sound insulation performance of building floors and meet the objective requirements for living comfort of residents,in this article,three kinds of elastic cushion materials,Portuguese cork board,BGL insulation sound insulation foam board,and EPP polypropylene plastic foam board,are applied to the sound insulation of a light frame wood floor structure of the same bedroom and compared to the ordinary floor.This study uses the transfer function method and transient excitation method to measure the sound insulation,damping ratio,and elastic modulus of materials,as well as the sound insulation of the floor under the jumping excitation method of daily behavior.Through comparative analysis,the results and factors of improving the sound insulation performance of the floor are obtained,according to which three types of elastic cushion materials and the floor covering composed of them have higher vibration and noise reduction performance.Among them,the overall sound insulation performance of BGL board floor is the highest,followed by EPP board and cork board floor,and ordinary OSB floor is the lowest.Under the jumping excitation method,three floating floors can improve the impact sound insulation performance of the middle and low-frequency bands.

Clinical antitumor application and pharmacological mechanisms of Dahuang Zhechong Pill

Cancer still has elevated morbidity and mortality,which undoubtedly impacts the life quality of affected individuals.Remarkable advances have been made in cancer therapy,although the toxicities of traditional therapies remain an obvious challenge.Dahuang Zhechong Pill(DHZCP),developed by Zhongjing Zhang in the Synopsis of the Golden Chamber,represents an effective anticancer traditional Chinese medicine(TCM).In this review,it was found that DHZCP is therapeutically utilized in liver,lung,gastric,pancreatic and other cancers in clinic.Pharmacological evidence showed that its anti-tumor mechanisms mainly involve induced cell cycle arrest,apoptosis and autophagy,as well as suppressed tumor cell proliferation,obstructed angiogenesis and metastasis,enhanced immunity,and reversal of multidrug resistance.The present review provides a solid basis for the clinical application of DHZCP and may promote the wide use of TCM in clinical antitumor application.

Continuous polypyrrole nanotubes encapsulated Co_(3)O_(4)nanoparticles with oxygen vacancies and electron transport channels boosting peroxymonosulfate activation

Co_(3)O_(4)particles are promising heterogeneous catalysts for peroxymonosulfate(PMS)activation;whereas they still surfer from the extensive agglomeration,serious Co leaching,poor electronic conductivity,and difficult recovery.Herein,a novel hybrid nanoarchitectonic constructed by encapsulating Co_(3)O_(4)nanoparticles into continuous polypyrrole(PPy)nanotubes(Co_(3)O_(4)@PPy hybrids)was developed using electrospun fibers as the templates,which boosted the catalytic degradation toward tetracycline(TC).The continuous polypyrrole nanotubes could provide the confined spaces,offer effective electron transfer pathway,suppress cobalt ion loss,facilitate the oxygen vacancy(Ovac)formation,and accelerate the Co^(2+)/Co^(3+)cycles.Co_(3)O_(4)@PPy hybrids thereby exhibited a remarkably enhanced catalytic activity with the TC degradation efficiency of 97.2%(kobs=0.244 min^(−1))within 20 min and total organic carbon(TOC)removal rate of 66.8%.Furthermore,the recycle test,real natural water treatment,and fluidized-column catalytic experiments indicated the potential of Co_(3)O_(4)@PPy hybrids in the practical large-scale applications.

Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

Primary ciliary dyskinesia(PCD)is a highly heterogeneous recessive inherited disorder.FAP54,the homolog of CFAP54 in Chlamydomonas reinhardtii,was previously demonstrated as the C1d projection of the central microtubule apparatus of flagella.A Cfap54 knockout mouse model was then reported to have PCD-relevant phenotypes.Through whole-exome sequencing,compound heterozygous variants c.2649_2657delinC(p.E883Dfs*47)and c.7312_7313insCGCAGGCTGAATTCTTGG(p.T2438delinsTQAEFLA)in a new suspected PCD-relevant gene,CFAP54,were identified in an individual with PCD.Two missense variants,c.4112A>C(p.E1371A)and c.6559C>T(p.P2187S),in CFAP54 were detected in another unrelated patient.In this study,a minigene assay was conducted on the frameshift mutation showing a reduction in mRNA expression.In addition,a CFAP54 in-frame variant knock-in mouse model was established,which recapitulated the typical symptoms of PCD,including hydrocephalus,infertility,and mucus accumulation in nasal sinuses.Correspondingly,two missense variants were deleterious,with a dramatic reduction in mRNA abundance from bronchial tissue and sperm.The identification of PCD-causing variants of CFAP54 in two unrelated patients with PCD for the first time provides strong supportive evidence that CFAP54 is a new PCD-causing gene.This study further helps expand the disease-associated gene spectrum and improve genetic testing for PCD diagnosis in the future.

Angong Niuhuang Pill ameliorates cerebral ischemic injury in mice by selectively activating mitophagy

Background:Angong Niuhuang Pill(ANP)is a famous Chinese materia medica preparation prescribed for treating ischemic stroke.However,the protective mechanisms of ANP in acute ischemic stroke remain unclear and need to be explored.Objective:Our study aims to investigate the therapeutic effects of ANP on focal cerebral ischemia and explore the molecular mechanisms underlying its heat-clearing and detoxifying effects.Methods:The permanent middle cerebral artery occlusion(pMCAO)model was established in C57BL/6 male mice,which were then treated with ANP(25 mg/kg,100 mg/kg,or 400 mg/kg)or 80 mg/kg butylphthalide.To further verify the involvement of mitophagy in ANP function,mice were also treated with the autophagy inhibitor wortmannin or the mitophagy inhibitor mdivi-1 at ischemic onset.Results:Acute administration with 100 mg/kg,or 400 mg/kg ANP significantly improved neurological behavior and decreased cerebral infarct volume,suggesting that ANP conferred neuroprotective effects dose-dependently.Further,western blot analysis showed that ANP treatment significantly decreased the protein levels of cytoplas-mic cytochrome C and Bax,but elevated levels of mitochondrial cytochrome C and Bcl-2.TUNEL staining also indicated that ANP ameliorated cell apoptosis.ANP did not promote ischemia-induced lipidation of LC3,but significantly reduced the protein level of SQSTM1.Moreover,ANP further reduced mitochondrial contents and enhanced translocation of Parkin and SQSTM1 to the mitochondria.Importantly,the neuroprotective effects of ANP could be abolished by wortmannin and mdivi-1,respectively.Conclusion:Our findings showed that ANP exerted protective effects in response to ischemic injury and enhanced selective mitophagy without increasing autophagic flux.

鼻窦炎-中耳炎-双肺弥漫支气管扩张

原发性纤毛运动障碍(PCD)是一种罕见的常染色体隐性或X连锁相关的双等位基因变异遗传病,以纤毛运动障碍为特征。典型表现包括支气管扩张、分泌性中耳炎、鼻窦炎、内脏反位和不孕不育等,常因临床表现相似需与囊性纤维化(CF)鉴别。本文介绍1例幼年起反复咳嗽、咳痰伴发热的青少年女性,有分泌性中耳炎、鼻窦炎病史,曾考虑诊断CF。后经基因测序发现PCD相关致病基因复合杂合变异,结合临床表现、影像学特点,最终诊断为PCD。

In situ preparation of g-C3N4/Bi4O5I2 complex and its elevated photoactivity in Methyl Orange degradation under visible light

A graphite carbon nitride(g-C3N4)modified Bi4O5I2 composite was successfully prepared insitu via the thermal treatment of a g-C3N4/Bi OI precursor at 400°C for 3 hr.The as-prepared g-C3N4/Bi4O5I2 showed high photocatalytic performance in Methyl Orange(MO)degradation under visible light.The best sample presented a degradation rate of 0.164 min^-1,which is 3.2 and 82 times as high as that of Bi4O5I2 and g-C3N4,respectively.The g-C3N4/Bi4O5I2 was characterized by X-ray powder diffractometer(XRD),scanning electron microscopy(SEM),transmission electron microscopy(TEM),Raman,X-ray photoelectron spectroscopy(XPS),ultraviolet-visible diffuse reflectance spectra(DRS),electrochemical impedance spectroscopy(EIS)and transient photocurrent response in order to explain the enhanced photoactivity.Results indicated that the decoration with a small amount of g-C3N4 influenced the specific surface area only slightly.Nevertheless,the capability for absorbing visible light was improved measurably,which was beneficial to the MO degradation.On top of that,a strong interaction between g-C3N4 and Bi4O5I2 was detected.This interplay promoted the formation of a favorable heterojunction structure and thereby enhanced the charge separation.Thus,the g-C3N4/Bi4O5I2 composite presented greater charge separation efficiency and much better photocatalytic performance than Bi4O5I2.Additionally,g-C3N4/Bi4O5I2 also presented high stability.·O2^- and holes were verified to be the main reactive species.

Nanobody-based chimeric antigen receptor T cells designed by CRISPR/Cas9 technology for solid tumor immunotherapy

Chimeric antigen receptor-based T-cell immunotherapy is a promising strategy for treatment of hematological malignant tumors;however,its efficacy towards solid cancer remains challenging.We therefore focused on developing nanobody-based CAR-T cells that treat the solid tumor.CD105 expression is upregulated on neoangiogenic endothelial and cancer cells.CD105 has been developed as a drug target.Here we show the generation of a CD105-specific nanobody,an anti-human CD105 CAR-T cells,by inserting the sequences for anti-CD105 nanobody-linked standard cassette genes into AAVS1 site using CRISPR/Cas9 technology.Co-culture with CD105+target cells led to the activation of anti-CD105 CAR-T cells that displayed the typically activated cytotoxic T-cell characters,ability to proliferate,the production of pro-inflammatory cytokines,and the specific killing efficacy against CD105+target cells in vitro.The in vivo treatment with anti-CD105 CAR-T cells significantly inhibited the growth of implanted CD105+tumors,reduced tumor weight,and prolonged the survival time of tumor-bearing NOD/SCID mice.Nanobody-based CAR-T cells can therefore function as an antitumor agent in human tumor xenograft models.Our findings determined that the strategy of nanobody-based CAR-T cells engineered by CRISPR/Cas9 system has a certain potential to treat solid tumor through targeting CD105 antigen.

Amorphous carbon-based materials as platform for advanced highperformance anodes in lithium secondary batteries

The growing concern for the exhaustion of fossil energy and the rapid revolution of electronics have created a rising demand for electrical energy storage devices with high energy density,for example,lithium secondary batteries(LSBs).With high surface area,low cost,excellent mechanical strength,and electrochemical stability,amorphous carbon-based materials(ACMs)have been widely investigated as promising platform for anode materials in the LSBs.In this review,we firstly summarize recent advances in the synthesis of the ACMs with various morphologies,ranging from zero-to three-dimensional structures.Then,the use of ACMs in Li-ion batteries and Li metal batteries is discussed respectively with the focus on the relationship between the structural features of the as-prepared ACMs and their roles in promoting electrochemical performances.Finally,the remaining challenges and the possible prospects for the use of ACMs in the LSBs are proposed to provide some useful clews for the future developments of this attractive area.

Case report of neurofibromatosis type 1 combined with primary ciliary dyskinesia

Neurofibromatosis(NF)is a genetic disease in which the lungs are rarely involved.However,in NF cases with lung involvement,chest computed tomography may show bilateral basal reticulations,apical bullae,and cysts without bronchiectasis.Herein,we report a patient diagnosed with NF on the basis of the results of genetic testing who presented with early-onset wet cough and bronchiectasis.Considering the differential diagnosis of bronchiectasis combined with his early-onset wet cough,sinusitis,and sperm quality decline,we considered the possibility of primary ciliary dyskinesia(PCD).Further electron microscopy analysis of cilia and identification of homozygous mutations in the RSPH4A gene confirmed the diagnosis of PCD.Therefore,for patients with NF,when an image change exists in the lungs that does not correspond to NF,the possibility of other diagnoses,including PCD,must be considered.

A Novel Aminoalkylsilane Compound with Oligo(ethylene oxide) Units as Effective Additive for Improving Cyclability of Lithium-ion Batteries

A new aminoalkylsilane compound, （（2-（2-（N,N-dimethylamino）ethoxy）ethoxy） methyl）trimethylsilane （TMSC1N2） based on the oligo（ethylene oxide） chain end-capped with organosilicon functional group and alkylamine group on each end, was introduced as an electrolyte additive for lithium-ion batteries. Electrochemical performances of different volume ratios of TMSC1N2 in the baseline electrolyte were conducted through cyclic voltammetry, electrochemical impedance spectroscopy and galvanostatic charge/ discharge tests of lithium-ion batteries. With adding 5 vol.% TMSC1N2 to the baseline electrolyte （1 mol/L LiPF6 in ethylene carbonate and diethyl carbonate （EC：DEC = 1：1, in volume））, the capacity retention of LiFePO4/Li cells could be significantly improved from 74.7% to 90.8% after 130 cycles. Furthermore, TMSC1N2 showed good compatibility with graphite electrode and would not deteriorate the electrochemical performance of graphite/Li anode cells. These data suggested that TMSC1N2 could be utilized as an effective additive for lithium-ion batteries.

Deflection test and modal analysis of lightweight timber floors

In order to meet the objective requirements of the safety and comfort of the modern lightweight timber floors,and strengthen the research on the coupling performance of the lightweight timber floors vibration characteristics and the building comfort,this article discusses the floor of a two-story prefabricated lightweight timber building demonstration house.In this paper,the floor structure of a two-story light-weight wooden house has been carried out on structural calculation modal and experimental modal,static uniform load and concentrated load deflection value testing.The evaluation of the deflection value of the floor structure,the mode shape,the coupling of the fundamental frequency mode parameters,and the vibration comfort were also studied.The results show that the fundamental frequency simulation value,one-way modal test value and two-way modal test value of the floor structure all meet the requirements of BS-6472(BS6472-1:2008).That is,the floor structure is not lower than 8 Hz design requirements,and meets the frequency of BS-6472(BS6472-1:2008).The weighted root mean square acceleration is lower than the requirement of 0.45 m/s^(2);the first three natural frequencies of the floor structure calculated by the finite element simulation are 16.413,31.847 and 48.921 Hz,and the fundamental frequency mode is the bending vibration in the length and width directions.The second order is the bending mode in the length direction,and the third order is the bending mode in the width direction.The fundamental frequency of the two-way modal test of the floor structure is the first-order bending mode in the X direction;and the second-order natural frequency is the second-order bending vibration shape in the X direction.when the uniform load is mainly the weight of floor own,the simulated maximum deflection value is 1.0658 mm;the simulation is performed according to the standard value of 0.566 kN/m for the uniform load of the floor design,and the simulation is the largest.The maximum deflection value of the simulated floor is 1.47383 mm at its midpoint,which meets the requirements of National Building Code of Canada-2015(NBCC).The maximum deflection limit of the light wood structure floor system is lower than 3 m and the maximum deflection limit is 2 mm;the six deflection value test lines simulated under a concentrated load of 1 kN all present a parabolic distribution and are symmetrical.The above results has engineering application value for promoting the research on the vibration characteristics of the fabricated lightweight timber floors structure and its optimization design.

Entrainment of Astrocytic and Neuronal Ca^(2+) Population Dynamics During Information Processing of Working Memory in Mice

Astrocytes are increasingly recognized to play an active role in learning and memory,but whether neural inputs can trigger event-specific astrocytic Ca^(2+)dynamics in real time to participate in working memory remains unclear due to the difficulties in directly monitoring astrocytic Ca^(2+)dynamics in animals performing tasks.Here,using fiber photometry,we showed that population astrocytic Ca^(2+)dynamics in the hippocampus were gated by sensory inputs(centered at the turning point of the T-maze)and modified by the reward delivery during the encoding and retrieval phases.Notably,there was a strong inter-locked and antagonistic relationship between the astrocytic and neuronal Ca^(2+)dynamics with a 3-s phase difference.Furthermore,there was a robust synchronization of astrocytic Ca^(2+)at the population level among the hippocampus,medial prefrontal cortex,and striatum.The inter-locked,bidirectional communication between astrocytes and neurons at the population level may contribute to the modulation of information processing in working memory.

Pseudomonas sp.ZXY-1,a newly isolated and highly efficient atrazine-degrading bacterium,and optimization of biodegradation using response surface methodology

Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model（R^2= 0.9821） being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions（30.71°C, pH 7.14, 4.23%（V/V） inoculum size and 157.1 mg/L initial atrazine concentration）.Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.

Nanostructured NiMoO_4 as active electrocatalyst for oxygen evolution

It is desirable to exploit cost-effective and earth-abundant catalysts for the oxygen evolution reaction (OER) in developing electrochemical energy conversion and storage technologies. Here we report a facile hydrothermal synthesis of NiMo04 nanorods as active and stable OER catalyst in alkaline condition. The prepared NiMoO_4 nanorods exhibit a considerably low overpotential of 340 mV at the current density of10 mA/cm^2 and a low Tafel slope of 45.6 mV/dec, which is comparable to the benchmark Ru02.Furthermore, the performance of NiMoO_4 significantly surpasses binary NiO and MoO_3 oxides due to enhanced charge transfer and promoted formation of catalytically active Ni^(3+) species. The results highlight the importance of designing ternary oxides in oxygen electrocatalysis.

Novel mutation c.1210-3C>G in cis with a poly-T tract of 5T affects CFTR mRNA splicing in a Chinese patient with cystic fibrosis

Cystic fibrosis(CF)is a rare autosomal recessive disease with only one pathogenic gene cystic fibrosis transmembrane conductance regulator(CFTR).To identify the potential pathogenic mutations in a Chinese patient with CF,we conducted Sanger sequencing on the genomic DNA of the patient and his parents and detected all 27 coding exons of CFTR and their flanking intronic regions.The patient is a compound heterozygote of c.2909G>A,p.Gly970Asp in exon 18 and c.1210-3C>G in cis with a poly-T of 5T(T5)sequence,3 bp upstream in intron 9.The splicing effect of c.1210-3C>G was verified via minigene assay in vitro,indicating that wild-type plasmid containing c.1210-3C together with T7 sequence produced a normal transcript and partial exon 10-skipping-transcript,whereas mutant plasmid containing c.1210-3G in cis with T5 sequence caused almost all mRNA to skip exon 10.Overall,c.1210-3C>G,the newly identified pathogenic mutation in our patient,in combination with T5 sequence in cis,affects the CFTR gene splicing and produces nearly no normal transcript in vitro.Moreover,this patient carries a p.Gly970Asp mutation,thus confirming the high-frequency of this mutation in Chinese patients with CF.