Carbyne delivers various excellent properties for the existence of the larger number of sp-hybridized carbon atoms.Here,a 3D well-defined porous carbon material germanium-carbdiyne(Ge-CDY)which is comprised of only sp...Carbyne delivers various excellent properties for the existence of the larger number of sp-hybridized carbon atoms.Here,a 3D well-defined porous carbon material germanium-carbdiyne(Ge-CDY)which is comprised of only sp-hybridized carbon atoms bridging by Ge atoms has been developed and investigated.The unique diamond-like structure constructed by linear butadiyne bonds and sp 3-hybridized Ge atoms ensures the stability of Ge-CDY.The large percentage of conjugated alkyne bonds composed of sp-C guarantees the good conductivity and the low band gap,which were further confirmed experimentally and theoretically,endowing Ge-CDY with the potential in electrochemical applications.The well-defined 3D carbon skeleton of Ge-CDY provides abundant uniform nanopores,which is suitable for metal ions storage and diffusion.Further half-cell evaluation also demonstrated Ge-CDY exhibited an excellent performance in lithium storage.All those indicating sp-hybridized carbon-based materials can exhibit great potential to possess excellent properties and be applied in the field of energy,electronic,and so on.展开更多
Global warming poses a serious threat to crops.Calcium-dependent protein kinases(CDPKs)/CPKs play vital roles in plant stress responses,but their exact roles in plant thermotolerance remains elusive.Here,we explored t...Global warming poses a serious threat to crops.Calcium-dependent protein kinases(CDPKs)/CPKs play vital roles in plant stress responses,but their exact roles in plant thermotolerance remains elusive.Here,we explored the roles of heat-induced ZmCDPK7 in thermotolerance in maize.ZmCDPK7-overexpressing maize plants displayed higher thermotolerance,photosynthetic rates,and antioxidant enzyme activity but lower H2 O2 and malondialdehyde(MDA)contents than wild-type plants under heat stress.ZmCDPK7-knockdown plants displayed the opposite patterns.ZmCDPK7 is attached to the plasma membrane but can translocate to the cytosol under heat stress.ZmCDPK7 interacts with the small heat shock protein sHSP17.4,phosphorylates sHSP17.4 at Ser-44 and the respiratory burst oxidase homolog RBOHB at Ser-99,and up regulates their expression.Site-directed mutagenesis of sHSP17.4 to generate a Ser-44-Ala substitution reduced ZmCDPK7’s enhancement of catalase activity but enhanced ZmCDPK7’s suppression of MDA accumulation in heat-stressed maize protoplasts.sHSP17.4,ZmCDPK7,and RBOHB were less strongly upregulated in response to heat stress in the abscisic acid-deficient mutant vp5 versus the wild type.Pretreatment with an RBOH inhibitor suppressed sHSP17.4 and ZmCDPK7 expression.Therefore,abscisic acid-induced ZmCDPK7 functions both upstream and downstream of RBOH and participates in thermotolerance in maize by mediating the phosphorylation of sHSP17.4,which might be essential for its chaperone function.展开更多
In spired by the clinically approved albumin based PTX formulatio n(Abraxa ne)and high-drug-loading dimeric prodrug tactics,herein we report a theranostic HAbraxane-likeH prodrug formulation,which is comprised of huma...In spired by the clinically approved albumin based PTX formulatio n(Abraxa ne)and high-drug-loading dimeric prodrug tactics,herein we report a theranostic HAbraxane-likeH prodrug formulation,which is comprised of human serum albumin(HSA),a paclitaxel(PTX)dimer bridged with thioether liner(PTX2-S),and photosensitizer IR780 iodide.Nanoparticles(NPs)with PTX2-S and IR780 as the core and HSA as the stealth shell are formed.Compared with HSA-based PTX clinical formulation(Abraxane),the dimeric molecules not only constitute the bulk structure of the particles,but also act as crossi ng age nt,thus realizing drug loadi ng content in creasi ng from 6.6 wt.%to 48.7 wt.%with high loadi ng efficie ncy(>90%)and excellent stability in biological conditions.Importa ntly,the thioether lin kage dually resp onds to the tumor redox heteroge neity and the NPs gradually releases the pare nt drug PTX for chemotherapy.Mea nwhile,PTX2-S facilitates the en capsulatio n of IR780 iodide due to their π-π stacki ng interaction and IR780 iodide gen erates spatio-temporal hyperthermia un der light irradiation to kill cancer cells for photothermal therapy.The described craft integrates the biomimetic trait of HSA,high drug loading,tumor redox heterogeneity-initiated on-dema nd drug release,and combi nation therapy into one formulati on and the developed nan oparticles are promising for cancer treatment.展开更多
Light utilization is one of the key factors for the improvement of photocatalytic perfo rmance.He rein,we design C-TiO_(2) hollow nanoshells with strong Mie resonance for enhanced photocatalytic hydrogen evolution in ...Light utilization is one of the key factors for the improvement of photocatalytic perfo rmance.He rein,we design C-TiO_(2) hollow nanoshells with strong Mie resonance for enhanced photocatalytic hydrogen evolution in a dye-sensitized system under visible light irradiation(λ≥420 nm).By tuning the inner diameters of hollow nanoshells,the Mie resonance in hollow nanoshells is adjusted for better excitation of dye molecules,which thus greatly enhances the light utilization in visible light region.This work shows the potential of Mie resonance in nanoshells can be an alternative strategy to increase the light utilization for photocatalysis.展开更多
1.Introduction Chemotherapy is the traditional treatment for clinical cancer patient[1,2].However,the therapeutic effect is often severely limited by the multidrug resistance(MDR)[3,4],which was commonly mediated by i...1.Introduction Chemotherapy is the traditional treatment for clinical cancer patient[1,2].However,the therapeutic effect is often severely limited by the multidrug resistance(MDR)[3,4],which was commonly mediated by inactivating drugs,increasing efflux,stimulating DNA repair mechanisms,and/or activating detoxification pathways[5-7].Recent studies are committing to develop reasonable strategies for intervention of the molecular pathways mediating MDR,of which P-glycoprotein(P-gp)has been widely investigated[8].So far,several kinds of P-gp inhibitors,including verapamil[9],vitamin E[10],cyclosporin A[11],and small interfering RNA(siRNA)[12].展开更多
基金This study was supported by the National Natural Science Foundation of China (21701182,51822208,21771187,21790050,and 21790051)the Frontier Science Research Project (QYZDB-SSW-JSC052)+1 种基金the Chinese Academy of Sciences,the Taishan Scholars Program of Shandong Province (tsqn201812111)Institute Research Project (QIBEBT ZZBS 201809).
文摘Carbyne delivers various excellent properties for the existence of the larger number of sp-hybridized carbon atoms.Here,a 3D well-defined porous carbon material germanium-carbdiyne(Ge-CDY)which is comprised of only sp-hybridized carbon atoms bridging by Ge atoms has been developed and investigated.The unique diamond-like structure constructed by linear butadiyne bonds and sp 3-hybridized Ge atoms ensures the stability of Ge-CDY.The large percentage of conjugated alkyne bonds composed of sp-C guarantees the good conductivity and the low band gap,which were further confirmed experimentally and theoretically,endowing Ge-CDY with the potential in electrochemical applications.The well-defined 3D carbon skeleton of Ge-CDY provides abundant uniform nanopores,which is suitable for metal ions storage and diffusion.Further half-cell evaluation also demonstrated Ge-CDY exhibited an excellent performance in lithium storage.All those indicating sp-hybridized carbon-based materials can exhibit great potential to possess excellent properties and be applied in the field of energy,electronic,and so on.
基金supported by the National Natural Science Foundation of Henan Joint Fund(U1604101 to XLH)the Key R&D and Promotion Projects of Henan Province(192102110009 to XLH,192102110004 to HY)。
文摘Global warming poses a serious threat to crops.Calcium-dependent protein kinases(CDPKs)/CPKs play vital roles in plant stress responses,but their exact roles in plant thermotolerance remains elusive.Here,we explored the roles of heat-induced ZmCDPK7 in thermotolerance in maize.ZmCDPK7-overexpressing maize plants displayed higher thermotolerance,photosynthetic rates,and antioxidant enzyme activity but lower H2 O2 and malondialdehyde(MDA)contents than wild-type plants under heat stress.ZmCDPK7-knockdown plants displayed the opposite patterns.ZmCDPK7 is attached to the plasma membrane but can translocate to the cytosol under heat stress.ZmCDPK7 interacts with the small heat shock protein sHSP17.4,phosphorylates sHSP17.4 at Ser-44 and the respiratory burst oxidase homolog RBOHB at Ser-99,and up regulates their expression.Site-directed mutagenesis of sHSP17.4 to generate a Ser-44-Ala substitution reduced ZmCDPK7’s enhancement of catalase activity but enhanced ZmCDPK7’s suppression of MDA accumulation in heat-stressed maize protoplasts.sHSP17.4,ZmCDPK7,and RBOHB were less strongly upregulated in response to heat stress in the abscisic acid-deficient mutant vp5 versus the wild type.Pretreatment with an RBOH inhibitor suppressed sHSP17.4 and ZmCDPK7 expression.Therefore,abscisic acid-induced ZmCDPK7 functions both upstream and downstream of RBOH and participates in thermotolerance in maize by mediating the phosphorylation of sHSP17.4,which might be essential for its chaperone function.
基金National Natural Science Foundation of China (Nos. 51773197 and 51522307).
文摘In spired by the clinically approved albumin based PTX formulatio n(Abraxa ne)and high-drug-loading dimeric prodrug tactics,herein we report a theranostic HAbraxane-likeH prodrug formulation,which is comprised of human serum albumin(HSA),a paclitaxel(PTX)dimer bridged with thioether liner(PTX2-S),and photosensitizer IR780 iodide.Nanoparticles(NPs)with PTX2-S and IR780 as the core and HSA as the stealth shell are formed.Compared with HSA-based PTX clinical formulation(Abraxane),the dimeric molecules not only constitute the bulk structure of the particles,but also act as crossi ng age nt,thus realizing drug loadi ng content in creasi ng from 6.6 wt.%to 48.7 wt.%with high loadi ng efficie ncy(>90%)and excellent stability in biological conditions.Importa ntly,the thioether lin kage dually resp onds to the tumor redox heteroge neity and the NPs gradually releases the pare nt drug PTX for chemotherapy.Mea nwhile,PTX2-S facilitates the en capsulatio n of IR780 iodide due to their π-π stacki ng interaction and IR780 iodide gen erates spatio-temporal hyperthermia un der light irradiation to kill cancer cells for photothermal therapy.The described craft integrates the biomimetic trait of HSA,high drug loading,tumor redox heterogeneity-initiated on-dema nd drug release,and combi nation therapy into one formulati on and the developed nan oparticles are promising for cancer treatment.
基金Financial support for this project was provided by the National Natural Science Foundation of China (Nos.51702023,51702022)Natural Science Research of Jiangsu Higher Education Institutions of China (No.17KJB430001)。
文摘Light utilization is one of the key factors for the improvement of photocatalytic perfo rmance.He rein,we design C-TiO_(2) hollow nanoshells with strong Mie resonance for enhanced photocatalytic hydrogen evolution in a dye-sensitized system under visible light irradiation(λ≥420 nm).By tuning the inner diameters of hollow nanoshells,the Mie resonance in hollow nanoshells is adjusted for better excitation of dye molecules,which thus greatly enhances the light utilization in visible light region.This work shows the potential of Mie resonance in nanoshells can be an alternative strategy to increase the light utilization for photocatalysis.
基金We acknowledge the financial support from the National Natural Science Foundation of China(No.U20A20260,51973213)Natural Science Foundation of Hebei Province(C20202006)。
文摘1.Introduction Chemotherapy is the traditional treatment for clinical cancer patient[1,2].However,the therapeutic effect is often severely limited by the multidrug resistance(MDR)[3,4],which was commonly mediated by inactivating drugs,increasing efflux,stimulating DNA repair mechanisms,and/or activating detoxification pathways[5-7].Recent studies are committing to develop reasonable strategies for intervention of the molecular pathways mediating MDR,of which P-glycoprotein(P-gp)has been widely investigated[8].So far,several kinds of P-gp inhibitors,including verapamil[9],vitamin E[10],cyclosporin A[11],and small interfering RNA(siRNA)[12].
