通过水包油乳化作用将卵磷脂来源的脂质体分散在水相,高浓度抗坏血酸四异棕榈酸酯(VCIP,Vitamin C tetra-isopalmitate)分散在油相制备成一定剂型的制剂,避免了脂质体包裹导致的稳定性问题和低载药量问题。实验结果表明,10%VCIP与不同...通过水包油乳化作用将卵磷脂来源的脂质体分散在水相,高浓度抗坏血酸四异棕榈酸酯(VCIP,Vitamin C tetra-isopalmitate)分散在油相制备成一定剂型的制剂,避免了脂质体包裹导致的稳定性问题和低载药量问题。实验结果表明,10%VCIP与不同含量的脂质体进行乳化作用后,透皮吸收作用加强。随着脂质体含量增加,VCIP的渗透性先增强后趋于平稳。在该制剂抗H_(2)O_(2)氧化损伤、促进成纤维细胞增殖、胶原蛋白相关基因表达、抗黑色素试验以及抑制基质金属蛋白酶基因表达实验表明,含脂质体的乳化制剂比不含脂质体的制剂功效要强,当脂质体含量大于等于0.5%时,呈极显著性差异。该研究在化妆品透皮吸收制剂中有一定的指导意义。展开更多
objective:The effects of different chitosan on preventing traumatic peritoneal adhesion in rats was studied in this paper. METHODS: 96 SD rats with injured vermiform process were randomly divided into 4 groups as foll...objective:The effects of different chitosan on preventing traumatic peritoneal adhesion in rats was studied in this paper. METHODS: 96 SD rats with injured vermiform process were randomly divided into 4 groups as follows: group A without any treatment as control, group B treated with chitosan gel, group C treated with pure chitosan film and group D treated with chiston film containing 50% gelatin. 2 and 4 weeks after surgery, 12 rats in each group were respectively belly opened to observe chitosan degradation and evaluate peritoneal adhesion, and the adhesive vermiform processes tissues were histopathologically observed. RESULTS: 1.Degradation in the group D was faster than that in the group C but slower than that in the group B. 2. 2 weeks after surgery the adhesion in the group B was milder than that in the control group(goup A) (P<0.05), but that in the group C and D (both P<0.05) were more severe than that in the control group . 3. 4 weeks after surgery , the adhesion in the group B was milder than that in the control group (P<0.05), but that in the group C and D (both P<0.05) were more severe than that in the control group , whereas, there was no significant difference between adhesion in the group C and group D (P>0.05). 4.Histopathological examinaiton indicated that: 2 weeks after surgery ,inflammatory cell infiltration and fibroplastic proliferation dominated in local lesion and the response was most severe on the serous coat, furthermore, the response in the control group was more severe than that in the group B, but milder than that in the group C and D; 4 weeks after surgery, fibroplastic proliferation dominated in local lesion in each group , moreover, the response in the control group was more severe than that in the group B but milder than that in the group C and D. What’s more, integrated fibrous membrane formed around implanted materials in the group C and D, and the fibrous membranes were thinner in the group C than that in the group D. CONCLUSION: 1.Chitosan gel has perfect effect in protecting traumatic peritoneal adhesion in rats. 2.Pure chitosan film could exacerbate peritoneal adhesion and chitosan containing gelatin could exacerbate peritoneal adhesion further.展开更多
Objective： To observe the clinical effect of acupuncture for persistent allergic rhinitis （PAR）. Methods： A total of 154 PAR patients were randomized into a treatment group and a control group, 78 cases in the tre...Objective： To observe the clinical effect of acupuncture for persistent allergic rhinitis （PAR）. Methods： A total of 154 PAR patients were randomized into a treatment group and a control group, 78 cases in the treatment group received acupuncture at meridian points, while 76 cases in the control group received acupuncture at non-meridian points. The treatment was done every other day, 3 times a week for a total 4 weeks. The total nasal symptom score （TNSS）, the total non-nasal symptom score （TNNSS） and the rhinoconjunctivitis quality of life questionnaire （RO, LQ） were measured before the treatment and after 2 weeks and 4 weeks of treatment, as well as 1 month and 3 months after the treatment to compare the clinical effect between the two groups. Results： After 4 weeks of treatment, the total effective rate in the treatment group was 92.3%, versus 76.3% in the control group, showing a statistically significant difference （P〈0.05）. Intra-group comparisons of TNSS, TNNSS and R0.L0. scores after 4 weeks of treatment showed statistically significant differences （all P〈0.05）. Between-group comparisons of TNSS, TNNSS and RQLQ score after 4 weeks of treatment and in follow-up visits showed statistically significant differences （all P〈0.05）. Conclusion： Acupuncture is effective for PAR, and acupuncture at meridian points can produce a better effect than acupuncture at non-meridian points.展开更多
文摘通过水包油乳化作用将卵磷脂来源的脂质体分散在水相,高浓度抗坏血酸四异棕榈酸酯(VCIP,Vitamin C tetra-isopalmitate)分散在油相制备成一定剂型的制剂,避免了脂质体包裹导致的稳定性问题和低载药量问题。实验结果表明,10%VCIP与不同含量的脂质体进行乳化作用后,透皮吸收作用加强。随着脂质体含量增加,VCIP的渗透性先增强后趋于平稳。在该制剂抗H_(2)O_(2)氧化损伤、促进成纤维细胞增殖、胶原蛋白相关基因表达、抗黑色素试验以及抑制基质金属蛋白酶基因表达实验表明,含脂质体的乳化制剂比不含脂质体的制剂功效要强,当脂质体含量大于等于0.5%时,呈极显著性差异。该研究在化妆品透皮吸收制剂中有一定的指导意义。
文摘objective:The effects of different chitosan on preventing traumatic peritoneal adhesion in rats was studied in this paper. METHODS: 96 SD rats with injured vermiform process were randomly divided into 4 groups as follows: group A without any treatment as control, group B treated with chitosan gel, group C treated with pure chitosan film and group D treated with chiston film containing 50% gelatin. 2 and 4 weeks after surgery, 12 rats in each group were respectively belly opened to observe chitosan degradation and evaluate peritoneal adhesion, and the adhesive vermiform processes tissues were histopathologically observed. RESULTS: 1.Degradation in the group D was faster than that in the group C but slower than that in the group B. 2. 2 weeks after surgery the adhesion in the group B was milder than that in the control group(goup A) (P<0.05), but that in the group C and D (both P<0.05) were more severe than that in the control group . 3. 4 weeks after surgery , the adhesion in the group B was milder than that in the control group (P<0.05), but that in the group C and D (both P<0.05) were more severe than that in the control group , whereas, there was no significant difference between adhesion in the group C and group D (P>0.05). 4.Histopathological examinaiton indicated that: 2 weeks after surgery ,inflammatory cell infiltration and fibroplastic proliferation dominated in local lesion and the response was most severe on the serous coat, furthermore, the response in the control group was more severe than that in the group B, but milder than that in the group C and D; 4 weeks after surgery, fibroplastic proliferation dominated in local lesion in each group , moreover, the response in the control group was more severe than that in the group B but milder than that in the group C and D. What’s more, integrated fibrous membrane formed around implanted materials in the group C and D, and the fibrous membranes were thinner in the group C than that in the group D. CONCLUSION: 1.Chitosan gel has perfect effect in protecting traumatic peritoneal adhesion in rats. 2.Pure chitosan film could exacerbate peritoneal adhesion and chitosan containing gelatin could exacerbate peritoneal adhesion further.
文摘Objective： To observe the clinical effect of acupuncture for persistent allergic rhinitis （PAR）. Methods： A total of 154 PAR patients were randomized into a treatment group and a control group, 78 cases in the treatment group received acupuncture at meridian points, while 76 cases in the control group received acupuncture at non-meridian points. The treatment was done every other day, 3 times a week for a total 4 weeks. The total nasal symptom score （TNSS）, the total non-nasal symptom score （TNNSS） and the rhinoconjunctivitis quality of life questionnaire （RO, LQ） were measured before the treatment and after 2 weeks and 4 weeks of treatment, as well as 1 month and 3 months after the treatment to compare the clinical effect between the two groups. Results： After 4 weeks of treatment, the total effective rate in the treatment group was 92.3%, versus 76.3% in the control group, showing a statistically significant difference （P〈0.05）. Intra-group comparisons of TNSS, TNNSS and R0.L0. scores after 4 weeks of treatment showed statistically significant differences （all P〈0.05）. Between-group comparisons of TNSS, TNNSS and RQLQ score after 4 weeks of treatment and in follow-up visits showed statistically significant differences （all P〈0.05）. Conclusion： Acupuncture is effective for PAR, and acupuncture at meridian points can produce a better effect than acupuncture at non-meridian points.
