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Genetically modified pigs with CD163 point mutation are resistant to HP-PRRSV infection
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作者 Ying liu lin Yang +9 位作者 Hong-Yong Xiang Ming Niu Jia-Cheng Deng xue-yuan li Wen-Jie Hao Hong-Sheng Ou-Yang Tong-Yu liu Xiao-Chun Tang Da-Xin Pang Hong-Ming Yuan 《Zoological Research》 SCIE CSCD 2024年第4期833-844,共12页
Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine industry.Modifyin... Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine industry.Modifying the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in pigs.However,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth performance.Reducing the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant pigs.In the current study,we identified a specific functional amino acid in CD163 that influences PRRSV proliferation.Viral infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and entry.Furthermore,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to HP-PRRSV.Examination of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G pigs.These findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks. 展开更多
关键词 PRRSV CD163 Point mutation E529G PIGS
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Evaluation of the etiology and risk factors for maternal sepsis: A single center study in Guangzhou, China 被引量:2
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作者 lin lin Lu-Wen Ren +4 位作者 xue-yuan li Wen Sun Yan-Hong Chen Jing-Si Chen Dun-Jin Chen 《World Journal of Clinical Cases》 SCIE 2021年第26期7704-7716,共13页
BACKGROUND Maternal sepsis is a major cause of gestational morbidity and neonatal mortality worldwide and particularly in China.AIM To evaluate the etiology of maternal sepsis and further identify its risk factors.MET... BACKGROUND Maternal sepsis is a major cause of gestational morbidity and neonatal mortality worldwide and particularly in China.AIM To evaluate the etiology of maternal sepsis and further identify its risk factors.METHODS In this retrospective study,we evaluated 70698 obstetric patients who were admitted to the Third Affiliated Hospital of Guangzhou Medical University between January 1,2009 and June 30,2018.Subjects were divided into sepsis group and non-sepsis group based on the incidence of sepsis.Data about medical history(surgical and obstetric history)and demographic information were collected.The Mann-Whitney U test was used to compare patient age,gestational age and duration of hospitalization between the two groups.Univariate and multivariate logistic regression models were used to analyze the etiology and the risk factors for maternal sepsis.Unadjusted and adjusted odds ratios(OR)are reported.RESULTS A total of 561 of 70698 obstetric patients were diagnosed with infection;of the infected patients,492 had non-sepsis associated infection(87.7%),while 69 had sepsis(12.3%).The morbidity rate of maternal sepsis was 9.76/10000;the fatality rate in the sepsis group was 11.6%(8/69).Emergency admission(OR=2.183)or transfer(OR=2.870),irregular prenatal care(OR=2.953),labor induction(OR=4.665),cervical cerclage(OR=14.214),first trimester(OR=6.806)and second trimester(OR=2.09)were significant risk factors for maternal sepsis.CONCLUSION Mode of admission,poor prenatal care,labor induction,cervical cerclage,first trimester and second trimester pregnancy were risk factors for maternal sepsis.Escherichia coli was the most common causative organism for maternal sepsis,and the uterus was the most common site of infection. 展开更多
关键词 Maternal sepsis Sepsis-3 Infection Risk factors
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One-stage human acellular nerve allograft reconstruction for digital nerve defects
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作者 xue-yuan li Hao-liang Hu +4 位作者 Jian-rong Fei Xin Wang Tian-bing Wang Pei-xun Zhang Hong Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期95-98,共4页
Human acellular nerve allografts have a wide range of donor origin and can effectively avoid nerve injury in the donor area. Very little is known about one-stage reconstruction of digital nerve defects. The present st... Human acellular nerve allografts have a wide range of donor origin and can effectively avoid nerve injury in the donor area. Very little is known about one-stage reconstruction of digital nerve defects. The present study observed the feasibility and effectiveness of human acellular nerve allograft in the reconstruction of 〈 5-cm digital nerve defects within 6 hours after injury. A total of 15 cases of nerve injury, combined with nerve defects in 18 digits from the Department of Emergency were enrolled in this study. After dehridement, digital nerves were reconstructed using human acellular nerve allografts. The patients were followed up for 6-24 months after reconstruction. Mackinnon-Dellon static two-point discrimination results showed excellent and good rates of 89%. Semmes-Weinstein monofilament test demonstrated that light touch was normal, with an obvious improvement rate of 78%. These findings confirmed that human acellular nerve allograft for one-stage reconstruction of digital nerve defect after hand injury is feasible, which provides a novel trend for peripheral nerve reconstruction. 展开更多
关键词 nerve regeneration peripheral nerve ALLOGRAFT digital nerve nerve conduit nerve recon-struction nerve defect sensory nerve neural regeneration
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